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The map label for this gene is gloB

Identifier: 157369154

GI number: 157369154

Start: 1008855

End: 1009610

Strand: Reverse

Name: gloB

Synonym: Spro_0909

Alternate gene names: 157369154

Gene position: 1009610-1008855 (Counterclockwise)

Preceding gene: 157369156

Following gene: 157369153

Centisome position: 18.53

GC content: 47.49

Gene sequence:

>756_bases
ATGAATCTTATCAGCATTCCCGCCTTCCAGGACAATTACATTTGGTTATTGGATAACCAGCAGGGGCATTGCATTATTGT
CGATCCCGGTGAAGCACAGCCGGTATTGGAAACGCTGGATCGCCTGAACCTGACGCCTGACGCCATTGTGCTTACCCATC
ATCATCACGATCACGTTGGCGGCGTAGCGCAAATTGTGGCGCGTTATTCCGGGCTAAAAGTCTATGGGCCGCAGGAAACT
GCCGACAAGGGAGCCAACCACATCGTTCATGATGGCGAAACCATCGAAATTAACGGCCAGAAATTCGCCACAATCGCGGT
TCCCGGACATACCTTGGGCCATGTAGCATTCTATAGCGCACCTTATCTGTTCTGCGGAGACACTATTTTCTCTGCTGGCT
GCGGAAGGCTTTTTGAAGGCACCGCAGAGCAAATGTTCAACTCATTTCAACAGCTCGCGCAACTTCCCGATAACACCTTA
ATTTGTTGTGCGCATGAATATACGCTTTCAAATCTTAAGTTTGCGCGAGCTATTTTGCCGAAAGATCGACACATTGAAAC
ATATCAGCAACAAGTTGAGGCGTTACGGGCAAAAGGCCAATCCAGCGTACCCACGACGCTACAATTAGAGCGTAAAATTA
ACCTATTTTTACGTTGCCATGACACTGATTTACAAAGAGAATTAGGCTTTCATACGCCACCAGAGCATCTTCATTCGGTT
TTTTCCGAATTACGCCTGCGTAAAGACAACTTCTGA

Upstream 100 bases:

>100_bases
AACTCTGCCCAGGACTGTGGGCCGGTGAGCTTTTGTAGAGTATGGGCTGGTTTCATGATTTATTATCTTCTTTCAAGCCG
TTGCCAACGAGAGGTCCAAC

Downstream 100 bases:

>100_bases
GCTTTTAGTTGTGTTGTTTGGCGAAGCAAAGTATGATTGCTCGTCTTTTAAGCAACTACATTGACACACACATGAAGGCT
AAAGCGATATTTCTCGCCTC

Product: hydroxyacylglutathione hydrolase

Products: NA

Alternate protein names: Glyoxalase II; Glx II

Number of amino acids: Translated: 251; Mature: 251

Protein sequence:

>251_residues
MNLISIPAFQDNYIWLLDNQQGHCIIVDPGEAQPVLETLDRLNLTPDAIVLTHHHHDHVGGVAQIVARYSGLKVYGPQET
ADKGANHIVHDGETIEINGQKFATIAVPGHTLGHVAFYSAPYLFCGDTIFSAGCGRLFEGTAEQMFNSFQQLAQLPDNTL
ICCAHEYTLSNLKFARAILPKDRHIETYQQQVEALRAKGQSSVPTTLQLERKINLFLRCHDTDLQRELGFHTPPEHLHSV
FSELRLRKDNF

Sequences:

>Translated_251_residues
MNLISIPAFQDNYIWLLDNQQGHCIIVDPGEAQPVLETLDRLNLTPDAIVLTHHHHDHVGGVAQIVARYSGLKVYGPQET
ADKGANHIVHDGETIEINGQKFATIAVPGHTLGHVAFYSAPYLFCGDTIFSAGCGRLFEGTAEQMFNSFQQLAQLPDNTL
ICCAHEYTLSNLKFARAILPKDRHIETYQQQVEALRAKGQSSVPTTLQLERKINLFLRCHDTDLQRELGFHTPPEHLHSV
FSELRLRKDNF
>Mature_251_residues
MNLISIPAFQDNYIWLLDNQQGHCIIVDPGEAQPVLETLDRLNLTPDAIVLTHHHHDHVGGVAQIVARYSGLKVYGPQET
ADKGANHIVHDGETIEINGQKFATIAVPGHTLGHVAFYSAPYLFCGDTIFSAGCGRLFEGTAEQMFNSFQQLAQLPDNTL
ICCAHEYTLSNLKFARAILPKDRHIETYQQQVEALRAKGQSSVPTTLQLERKINLFLRCHDTDLQRELGFHTPPEHLHSV
FSELRLRKDNF

Specific function: Thiolesterase that catalyzes the hydrolysis of S-D- lactoyl-glutathione to form glutathione and D-lactic acid

COG id: COG0491

COG function: function code R; Zn-dependent hydrolases, including glyoxylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family

Homologues:

Organism=Homo sapiens, GI94538320, Length=260, Percent_Identity=33.8461538461538, Blast_Score=155, Evalue=3e-38,
Organism=Homo sapiens, GI94538322, Length=260, Percent_Identity=33.8461538461538, Blast_Score=155, Evalue=3e-38,
Organism=Homo sapiens, GI116642887, Length=237, Percent_Identity=34.5991561181435, Blast_Score=142, Evalue=4e-34,
Organism=Homo sapiens, GI21703352, Length=237, Percent_Identity=34.5991561181435, Blast_Score=142, Evalue=4e-34,
Organism=Homo sapiens, GI14150041, Length=251, Percent_Identity=31.4741035856574, Blast_Score=137, Evalue=6e-33,
Organism=Homo sapiens, GI46361987, Length=202, Percent_Identity=32.6732673267327, Blast_Score=121, Evalue=6e-28,
Organism=Escherichia coli, GI1786406, Length=251, Percent_Identity=56.5737051792829, Blast_Score=294, Evalue=4e-81,
Organism=Escherichia coli, GI1787158, Length=200, Percent_Identity=30.5, Blast_Score=76, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17536925, Length=236, Percent_Identity=31.3559322033898, Blast_Score=117, Evalue=4e-27,
Organism=Saccharomyces cerevisiae, GI6320478, Length=260, Percent_Identity=28.8461538461538, Blast_Score=94, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6324614, Length=264, Percent_Identity=28.030303030303, Blast_Score=80, Evalue=4e-16,
Organism=Drosophila melanogaster, GI21356335, Length=261, Percent_Identity=34.4827586206897, Blast_Score=159, Evalue=2e-39,
Organism=Drosophila melanogaster, GI24667711, Length=262, Percent_Identity=35.1145038167939, Blast_Score=159, Evalue=2e-39,
Organism=Drosophila melanogaster, GI24667703, Length=261, Percent_Identity=34.4827586206897, Blast_Score=159, Evalue=2e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLO2_SERP5 (A8GA75)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001477143.1
- ProteinModelPortal:   A8GA75
- SMR:   A8GA75
- STRING:   A8GA75
- GeneID:   5603796
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_0909
- eggNOG:   COG0491
- HOGENOM:   HBG753931
- OMA:   WCAHEYT
- ProtClustDB:   CLSK888727
- BioCyc:   SPRO399741:SPRO_0909-MONOMER
- HAMAP:   MF_01374
- InterPro:   IPR001279
- InterPro:   IPR017782
- SMART:   SM00849
- TIGRFAMs:   TIGR03413

Pfam domain/function: PF00753 Lactamase_B

EC number: =3.1.2.6

Molecular weight: Translated: 28173; Mature: 28173

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLISIPAFQDNYIWLLDNQQGHCIIVDPGEAQPVLETLDRLNLTPDAIVLTHHHHDHVG
CCEEECCCCCCCEEEEEECCCCCEEEECCCCCCHHHHHHHHCCCCCCEEEEEECCCCHHH
GVAQIVARYSGLKVYGPQETADKGANHIVHDGETIEINGQKFATIAVPGHTLGHVAFYSA
HHHHHHHHHCCEEEECCCHHHHCCCCCEEECCCEEEECCCEEEEEEECCCCHHHHHHHCC
PYLFCGDTIFSAGCGRLFEGTAEQMFNSFQQLAQLPDNTLICCAHEYTLSNLKFARAILP
CEEEECCHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHCC
KDRHIETYQQQVEALRAKGQSSVPTTLQLERKINLFLRCHDTDLQRELGFHTPPEHLHSV
CCCCHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEEEEEECCCCHHHHCCCCCCHHHHHHH
FSELRLRKDNF
HHHHHHHCCCC
>Mature Secondary Structure
MNLISIPAFQDNYIWLLDNQQGHCIIVDPGEAQPVLETLDRLNLTPDAIVLTHHHHDHVG
CCEEECCCCCCCEEEEEECCCCCEEEECCCCCCHHHHHHHHCCCCCCEEEEEECCCCHHH
GVAQIVARYSGLKVYGPQETADKGANHIVHDGETIEINGQKFATIAVPGHTLGHVAFYSA
HHHHHHHHHCCEEEECCCHHHHCCCCCEEECCCEEEECCCEEEEEEECCCCHHHHHHHCC
PYLFCGDTIFSAGCGRLFEGTAEQMFNSFQQLAQLPDNTLICCAHEYTLSNLKFARAILP
CEEEECCHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHCC
KDRHIETYQQQVEALRAKGQSSVPTTLQLERKINLFLRCHDTDLQRELGFHTPPEHLHSV
CCCCHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEEEEEECCCCHHHHCCCCCCHHHHHHH
FSELRLRKDNF
HHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA