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The map label for this gene is pucI [H]

Identifier: 157369158

GI number: 157369158

Start: 1011921

End: 1013381

Strand: Reverse

Name: pucI [H]

Synonym: Spro_0913

Alternate gene names: 157369158

Gene position: 1013381-1011921 (Counterclockwise)

Preceding gene: 157369162

Following gene: 157369156

Centisome position: 18.6

GC content: 56.06

Gene sequence:

>1461_bases
ATGCCAAACCCTAATGAAAACTACAGTCCCAAGCTGTGCAACGACGATCTGGCACCCACGCGTGACCAGAACTGGAGCTG
GTACAACATATTTTCTTTCTGGATGTCCGATGTACACAGCATGGGTGGCTATGTGGTGGCCGCCAGCTTTTTCGCCCTTG
GCCTGTCGAGTTGGCAGGTTTTGATTTGTCTGCTGGTGGGGATTTGCATCGTGCAGTTTTGCGCAAACCTGGTGGCTAAG
CCCAGCCAACTCGGCGGCGTGCCCTACGCCGTATTAAGCCGTCAGGCGTTTGGCGTGTTCGGCGCCAATATTCCGGCGGT
GATCCGCGGCATGATCGCCTTCGCCTGGTACGGCATCCAAACTTATCTGGCCTCCAACGCGTTGATGCTGACGCTGCTGA
AATTCTTCCCGTCGCTGGAACCGATGACCCAACCGCATTTCCTCGGGCTGTCGCAGCTTGGCTGGGGCTGTTACATCACC
ATGTGGCTGTTGCAGGCGATGGTGTTTTGGCACGGTATGCAGGCAATCAAGCGCTTTATCGATATCGCCGGACCGGCGGT
TTACGTGGTGATGTTTGCGCTGGCGGGCTGGATTGTTTATCGCGTCGGTTTCTCCAACCTTTCCTTCACGCTGTCGAGCA
AAAACCTGAGCACCGGCGAACAGGGCTGGCTGATGCTGACCGCGACCGCGCTGGTGGTGTCCTACTTCTCCGGCCCGCTG
CTTAACTTCGGCGACTTTACCCGCTACGGCAAAAGCTTTGCCGAAGTGCAGCGCGGTAACCGCTGGGGTTTGCCGTTCAA
CTATCTGCTGTTCTCATTGGTGACCGTGGTGATCGTTTCCGGCACCCTGCCGCTGTTTGGGCAACTGATCACCGACCCGA
TCGAAACCGTCACCCGCCTCGACAGCGATATTGCCGTCGCCCTGGGTTTGCTGACCATGATTGTCGCCACTATCGGCATC
AATATTGTGGCCAACTTTGTCTCACCGGCGTTTGATTTTTCCAACTGCTCGCCACAGAAGATCAGCTTCCGCACCGGCGG
GATGATCGCCGCCGTCGGCTCATTCCTGCTGACACCGTGGAACCTGTTCCAGTCACCCGAACTGATTCATTACACGCTGG
ACGTATTGGCTGCCTTTATCGGCCCGCTGTTCGGTATTCTGTTGGTCGACTTTTACATCATCAAGCGCGGTGAATTGCAC
GTCGACGATTTGTATAACGCCACGCCAAACGGCCGTTACTGGTACAAGAATGGGTTCAATCCGAATGCGATTTGGGCGCT
GGTGCCGTCAGTGGTGATCGGGTTGGCGATCAGTTTTACGCCGAGTCTGCATCAGGTGGCGAACTTCAGTTGGTTTATCG
GGGCGGCACTGGGTGGGGGATGTTATCGGATCATTGCCCGTCGCGAATGGGCAGCCAATCAGGCGGTAACCTTTGCCAAG
GCGGAATTAAGCAAAGATTAA

Upstream 100 bases:

>100_bases
AAGAGCAGTCTGGCCTGGATCCTGCAAATGCCCTCTCAGCAATCTTTGCCAAAAAACAGATAACAACATCCGGGTGACGT
TCTTGAGAGGAGCACGATTT

Downstream 100 bases:

>100_bases
AAACAACGAAGCCCTGAGTTATTACTAACTCAGGGCTTCTTAATGTTGGCGGAACGGACGGGGCTCGAACCCGCGACCCC
CTGCGTGACAGGCAGGTATT

Product: cytosine/purines uracil thiamine allantoin permease

Products: NA

Alternate protein names: Allantoin transport protein [H]

Number of amino acids: Translated: 486; Mature: 485

Protein sequence:

>486_residues
MPNPNENYSPKLCNDDLAPTRDQNWSWYNIFSFWMSDVHSMGGYVVAASFFALGLSSWQVLICLLVGICIVQFCANLVAK
PSQLGGVPYAVLSRQAFGVFGANIPAVIRGMIAFAWYGIQTYLASNALMLTLLKFFPSLEPMTQPHFLGLSQLGWGCYIT
MWLLQAMVFWHGMQAIKRFIDIAGPAVYVVMFALAGWIVYRVGFSNLSFTLSSKNLSTGEQGWLMLTATALVVSYFSGPL
LNFGDFTRYGKSFAEVQRGNRWGLPFNYLLFSLVTVVIVSGTLPLFGQLITDPIETVTRLDSDIAVALGLLTMIVATIGI
NIVANFVSPAFDFSNCSPQKISFRTGGMIAAVGSFLLTPWNLFQSPELIHYTLDVLAAFIGPLFGILLVDFYIIKRGELH
VDDLYNATPNGRYWYKNGFNPNAIWALVPSVVIGLAISFTPSLHQVANFSWFIGAALGGGCYRIIARREWAANQAVTFAK
AELSKD

Sequences:

>Translated_486_residues
MPNPNENYSPKLCNDDLAPTRDQNWSWYNIFSFWMSDVHSMGGYVVAASFFALGLSSWQVLICLLVGICIVQFCANLVAK
PSQLGGVPYAVLSRQAFGVFGANIPAVIRGMIAFAWYGIQTYLASNALMLTLLKFFPSLEPMTQPHFLGLSQLGWGCYIT
MWLLQAMVFWHGMQAIKRFIDIAGPAVYVVMFALAGWIVYRVGFSNLSFTLSSKNLSTGEQGWLMLTATALVVSYFSGPL
LNFGDFTRYGKSFAEVQRGNRWGLPFNYLLFSLVTVVIVSGTLPLFGQLITDPIETVTRLDSDIAVALGLLTMIVATIGI
NIVANFVSPAFDFSNCSPQKISFRTGGMIAAVGSFLLTPWNLFQSPELIHYTLDVLAAFIGPLFGILLVDFYIIKRGELH
VDDLYNATPNGRYWYKNGFNPNAIWALVPSVVIGLAISFTPSLHQVANFSWFIGAALGGGCYRIIARREWAANQAVTFAK
AELSKD
>Mature_485_residues
PNPNENYSPKLCNDDLAPTRDQNWSWYNIFSFWMSDVHSMGGYVVAASFFALGLSSWQVLICLLVGICIVQFCANLVAKP
SQLGGVPYAVLSRQAFGVFGANIPAVIRGMIAFAWYGIQTYLASNALMLTLLKFFPSLEPMTQPHFLGLSQLGWGCYITM
WLLQAMVFWHGMQAIKRFIDIAGPAVYVVMFALAGWIVYRVGFSNLSFTLSSKNLSTGEQGWLMLTATALVVSYFSGPLL
NFGDFTRYGKSFAEVQRGNRWGLPFNYLLFSLVTVVIVSGTLPLFGQLITDPIETVTRLDSDIAVALGLLTMIVATIGIN
IVANFVSPAFDFSNCSPQKISFRTGGMIAAVGSFLLTPWNLFQSPELIHYTLDVLAAFIGPLFGILLVDFYIIKRGELHV
DDLYNATPNGRYWYKNGFNPNAIWALVPSVVIGLAISFTPSLHQVANFSWFIGAALGGGCYRIIARREWAANQAVTFAKA
ELSKD

Specific function: Transport of allantoin [H]

COG id: COG1953

COG function: function code FH; Cytosine/uracil/thiamine/allantoin permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine-cytosine permease family [H]

Homologues:

Organism=Escherichia coli, GI87081749, Length=477, Percent_Identity=27.8825995807128, Blast_Score=219, Evalue=4e-58,
Organism=Saccharomyces cerevisiae, GI6319429, Length=457, Percent_Identity=24.72647702407, Blast_Score=134, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6319495, Length=435, Percent_Identity=22.7586206896552, Blast_Score=132, Evalue=9e-32,
Organism=Saccharomyces cerevisiae, GI6322219, Length=437, Percent_Identity=21.7391304347826, Blast_Score=128, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6324766, Length=459, Percent_Identity=20.479302832244, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6324645, Length=459, Percent_Identity=19.6078431372549, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6323266, Length=452, Percent_Identity=20.7964601769912, Blast_Score=71, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001248
- InterPro:   IPR012681 [H]

Pfam domain/function: PF02133 Transp_cyt_pur [H]

EC number: NA

Molecular weight: Translated: 53609; Mature: 53478

Theoretical pI: Translated: 8.03; Mature: 8.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHCCCC
>Mature Secondary Structure 
PNPNENYSPKLCNDDLAPTRDQNWSWYNIFSFWMSDVHSMGGYVVAASFFALGLSSWQV
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH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HHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377; 11344136 [H]