| Definition | Serratia proteamaculans 568 chromosome, complete genome. |
|---|---|
| Accession | NC_009832 |
| Length | 5,448,853 |
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The map label for this gene is dkgB [H]
Identifier: 157369149
GI number: 157369149
Start: 1003277
End: 1004080
Strand: Direct
Name: dkgB [H]
Synonym: Spro_0904
Alternate gene names: 157369149
Gene position: 1003277-1004080 (Clockwise)
Preceding gene: 157369148
Following gene: 157369151
Centisome position: 18.41
GC content: 55.85
Gene sequence:
>804_bases ATGAGCATCCCTGCATTTGGTTTAGGTACCTTCCGTCTTCAGGATCAGGTCGTCATCGACTCGGTTAGCACGGCGCTGGA GCTGGGGTATCGCGCCATTGATACTGCACAAATCTACGAAAATGAAGCTGCGGTGGGCCAGGCGATTGCTGCCAGCGGTG TGCCACGTGACGAGCTGTTTATCACCACCAAAATCTGGATCGCCAATCTGGCGAAGGGCAAGCTGATCCCAAGCCTGCGG GAGAGCCTGACTAAACTGCAAACCAGCTATGTTGACCTGACTCTGATCCACTGGCCTTCGCCGAATGACGAAGTCTCGGT GGCCGAGTTTATGACTGAGCTGGCGGAAGCCAAAAAACTGGGCCTGACCCGCCAGATTGGGGTCTCCAACTTCACTTTGG ATCTGATGCAACAGGCGATTGATGCCGTGGGTAGCGATCAGATCGCCACTAACCAAATCGAGCTGTCGCCATATCTGCAG AACCGCAAAGTGGTGGAATTTGCCCAGCAGCATGGCATCGCCATCACTTCCTATATGACGCTGGCCTATGGGCAGGCACT GCAGGATGAGACCATCAAGGCCATCGCGGCACGCCGCCATGCTACTCCGGCGCAGGTTATCCTGGCCTGGGCGCTGCAAC TGGGTTACGCGGTGATCCCATCATCAACCAAGCGTGAAAACCTGGCGAGCAACCTGTTGGCGCAACAGCTAACGTTGACC GCCGAGGAAATGGCGGAAATCGCCAAATTGGAAAACAATGGCCGTTTGGTCAGCCCGGAAGGTCTGGCACCTAACTGGGA TTGA
Upstream 100 bases:
>100_bases TTAATCAAAAGTCTTATGCACAATAGGGCGTTTTTGATCAACAAAACAGTTGCCATAATCCTCCCATATCGCTTCAACAT CCTAATGAGAGAATATAATT
Downstream 100 bases:
>100_bases TTTTCGACGGCATACGCATTGAGGCGCCTAAACGGGCGCCTTTTTTATTGCGCCAATCGCTCACTGATAAAATCGATAAA GCTGCGCAGGCGATTGCTGA
Product: 2,5-diketo-D-gluconate reductase B
Products: NA
Alternate protein names: 2,5-DKG reductase B; 2,5-DKGR B; 25DKGR-B; AKR5D [H]
Number of amino acids: Translated: 267; Mature: 266
Protein sequence:
>267_residues MSIPAFGLGTFRLQDQVVIDSVSTALELGYRAIDTAQIYENEAAVGQAIAASGVPRDELFITTKIWIANLAKGKLIPSLR ESLTKLQTSYVDLTLIHWPSPNDEVSVAEFMTELAEAKKLGLTRQIGVSNFTLDLMQQAIDAVGSDQIATNQIELSPYLQ NRKVVEFAQQHGIAITSYMTLAYGQALQDETIKAIAARRHATPAQVILAWALQLGYAVIPSSTKRENLASNLLAQQLTLT AEEMAEIAKLENNGRLVSPEGLAPNWD
Sequences:
>Translated_267_residues MSIPAFGLGTFRLQDQVVIDSVSTALELGYRAIDTAQIYENEAAVGQAIAASGVPRDELFITTKIWIANLAKGKLIPSLR ESLTKLQTSYVDLTLIHWPSPNDEVSVAEFMTELAEAKKLGLTRQIGVSNFTLDLMQQAIDAVGSDQIATNQIELSPYLQ NRKVVEFAQQHGIAITSYMTLAYGQALQDETIKAIAARRHATPAQVILAWALQLGYAVIPSSTKRENLASNLLAQQLTLT AEEMAEIAKLENNGRLVSPEGLAPNWD >Mature_266_residues SIPAFGLGTFRLQDQVVIDSVSTALELGYRAIDTAQIYENEAAVGQAIAASGVPRDELFITTKIWIANLAKGKLIPSLRE SLTKLQTSYVDLTLIHWPSPNDEVSVAEFMTELAEAKKLGLTRQIGVSNFTLDLMQQAIDAVGSDQIATNQIELSPYLQN RKVVEFAQQHGIAITSYMTLAYGQALQDETIKAIAARRHATPAQVILAWALQLGYAVIPSSTKRENLASNLLAQQLTLTA EEMAEIAKLENNGRLVSPEGLAPNWD
Specific function: Catalyzes the reduction of 2,5-diketo-D-gluconic acid (25DKG) to 2-keto-L-gulonic acid (2KLG) [H]
COG id: COG0656
COG function: function code R; Aldo/keto reductases, related to diketogulonate reductase
Gene ontology:
Cell location: Cytoplasm [H]
Metaboloic importance: Unknown [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the aldo/keto reductase family [H]
Homologues:
Organism=Homo sapiens, GI5174695, Length=293, Percent_Identity=32.0819112627986, Blast_Score=144, Evalue=9e-35, Organism=Homo sapiens, GI310109922, Length=286, Percent_Identity=32.1678321678322, Blast_Score=142, Evalue=2e-34, Organism=Homo sapiens, GI223468663, Length=289, Percent_Identity=31.4878892733564, Blast_Score=141, Evalue=5e-34, Organism=Homo sapiens, GI310109920, Length=303, Percent_Identity=30.6930693069307, Blast_Score=140, Evalue=1e-33, Organism=Homo sapiens, GI45446745, Length=303, Percent_Identity=30.6930693069307, Blast_Score=140, Evalue=1e-33, Organism=Homo sapiens, GI4503285, Length=303, Percent_Identity=30.6930693069307, Blast_Score=140, Evalue=1e-33, Organism=Homo sapiens, GI24497577, Length=290, Percent_Identity=30.3448275862069, Blast_Score=139, Evalue=3e-33, Organism=Homo sapiens, GI5174391, Length=290, Percent_Identity=30.3448275862069, Blast_Score=139, Evalue=3e-33, Organism=Homo sapiens, GI5453543, Length=303, Percent_Identity=30.3630363036304, Blast_Score=137, Evalue=9e-33, Organism=Homo sapiens, GI4502049, Length=289, Percent_Identity=31.8339100346021, Blast_Score=134, Evalue=7e-32, Organism=Homo sapiens, GI93277124, Length=299, Percent_Identity=30.4347826086957, Blast_Score=133, Evalue=1e-31, Organism=Homo sapiens, GI24497583, Length=303, Percent_Identity=29.7029702970297, Blast_Score=132, Evalue=3e-31, Organism=Homo sapiens, GI24497585, Length=291, Percent_Identity=30.2405498281787, Blast_Score=132, Evalue=3e-31, Organism=Homo sapiens, GI291291012, Length=273, Percent_Identity=30.7692307692308, Blast_Score=129, Evalue=2e-30, Organism=Homo sapiens, GI300116273, Length=268, Percent_Identity=31.3432835820896, Blast_Score=128, Evalue=4e-30, Organism=Homo sapiens, GI300116271, Length=278, Percent_Identity=31.294964028777, Blast_Score=124, Evalue=1e-28, Organism=Homo sapiens, GI310109924, Length=167, Percent_Identity=28.7425149700599, Blast_Score=79, Evalue=3e-15, Organism=Homo sapiens, GI207028673, Length=115, Percent_Identity=39.1304347826087, Blast_Score=76, Evalue=4e-14, Organism=Homo sapiens, GI310109926, Length=115, Percent_Identity=39.1304347826087, Blast_Score=75, Evalue=4e-14, Organism=Escherichia coli, GI1786400, Length=267, Percent_Identity=76.7790262172285, Blast_Score=416, Evalue=1e-117, Organism=Escherichia coli, GI87082198, Length=256, Percent_Identity=38.671875, Blast_Score=175, Evalue=3e-45, Organism=Escherichia coli, GI1788081, Length=268, Percent_Identity=29.8507462686567, Blast_Score=95, Evalue=5e-21, Organism=Escherichia coli, GI48994888, Length=281, Percent_Identity=28.8256227758007, Blast_Score=80, Evalue=2e-16, Organism=Escherichia coli, GI1787674, Length=252, Percent_Identity=28.1746031746032, Blast_Score=71, Evalue=1e-13, Organism=Escherichia coli, GI1788070, Length=216, Percent_Identity=27.7777777777778, Blast_Score=62, Evalue=3e-11, Organism=Caenorhabditis elegans, GI17550248, Length=286, Percent_Identity=38.4615384615385, Blast_Score=179, Evalue=1e-45, Organism=Caenorhabditis elegans, GI17537077, Length=288, Percent_Identity=35.7638888888889, Blast_Score=152, Evalue=2e-37, Organism=Caenorhabditis elegans, GI17537075, Length=290, Percent_Identity=35.1724137931034, Blast_Score=147, Evalue=6e-36, Organism=Caenorhabditis elegans, GI71998625, Length=266, Percent_Identity=30.0751879699248, Blast_Score=132, Evalue=2e-31, Organism=Caenorhabditis elegans, GI17561300, Length=274, Percent_Identity=30.2919708029197, Blast_Score=129, Evalue=2e-30, Organism=Caenorhabditis elegans, GI17552492, Length=265, Percent_Identity=29.811320754717, Blast_Score=127, Evalue=5e-30, Organism=Caenorhabditis elegans, GI17566692, Length=262, Percent_Identity=32.4427480916031, Blast_Score=124, Evalue=4e-29, Organism=Caenorhabditis elegans, GI17537079, Length=302, Percent_Identity=28.1456953642384, Blast_Score=123, Evalue=1e-28, Organism=Caenorhabditis elegans, GI17564128, Length=291, Percent_Identity=28.5223367697594, Blast_Score=120, Evalue=7e-28, Organism=Caenorhabditis elegans, GI17561298, Length=275, Percent_Identity=26.5454545454545, Blast_Score=116, Evalue=1e-26, Organism=Caenorhabditis elegans, GI17562292, Length=268, Percent_Identity=29.8507462686567, Blast_Score=113, Evalue=9e-26, Organism=Caenorhabditis elegans, GI17538386, Length=264, Percent_Identity=28.4090909090909, Blast_Score=110, Evalue=7e-25, Organism=Saccharomyces cerevisiae, GI6324694, Length=298, Percent_Identity=33.2214765100671, Blast_Score=158, Evalue=9e-40, Organism=Saccharomyces cerevisiae, GI6320576, Length=280, Percent_Identity=35, Blast_Score=156, Evalue=2e-39, Organism=Saccharomyces cerevisiae, GI6321896, Length=306, Percent_Identity=29.7385620915033, Blast_Score=131, Evalue=1e-31, Organism=Saccharomyces cerevisiae, GI6322556, Length=252, Percent_Identity=27.3809523809524, Blast_Score=120, Evalue=2e-28, Organism=Saccharomyces cerevisiae, GI6319625, Length=284, Percent_Identity=29.2253521126761, Blast_Score=107, Evalue=2e-24, Organism=Saccharomyces cerevisiae, GI6320079, Length=249, Percent_Identity=31.7269076305221, Blast_Score=104, Evalue=2e-23, Organism=Drosophila melanogaster, GI24644950, Length=296, Percent_Identity=33.7837837837838, Blast_Score=145, Evalue=3e-35, Organism=Drosophila melanogaster, GI221378297, Length=319, Percent_Identity=31.974921630094, Blast_Score=138, Evalue=5e-33, Organism=Drosophila melanogaster, GI24662789, Length=294, Percent_Identity=31.9727891156463, Blast_Score=137, Evalue=6e-33, Organism=Drosophila melanogaster, GI20129731, Length=286, Percent_Identity=32.8671328671329, Blast_Score=137, Evalue=1e-32, Organism=Drosophila melanogaster, GI24657054, Length=288, Percent_Identity=30.5555555555556, Blast_Score=129, Evalue=2e-30, Organism=Drosophila melanogaster, GI24662785, Length=290, Percent_Identity=35.1724137931034, Blast_Score=126, Evalue=1e-29, Organism=Drosophila melanogaster, GI45553081, Length=286, Percent_Identity=29.7202797202797, Blast_Score=125, Evalue=4e-29, Organism=Drosophila melanogaster, GI24663317, Length=285, Percent_Identity=29.4736842105263, Blast_Score=123, Evalue=2e-28, Organism=Drosophila melanogaster, GI24662781, Length=290, Percent_Identity=34.4827586206897, Blast_Score=122, Evalue=2e-28, Organism=Drosophila melanogaster, GI21356425, Length=285, Percent_Identity=28.7719298245614, Blast_Score=118, Evalue=4e-27, Organism=Drosophila melanogaster, GI281366140, Length=261, Percent_Identity=28.735632183908, Blast_Score=106, Evalue=2e-23,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR001395 - InterPro: IPR018170 - InterPro: IPR020471 - InterPro: IPR023210 [H]
Pfam domain/function: PF00248 Aldo_ket_red [H]
EC number: =1.1.1.274 [H]
Molecular weight: Translated: 29123; Mature: 28991
Theoretical pI: Translated: 4.62; Mature: 4.62
Prosite motif: PS00798 ALDOKETO_REDUCTASE_1 ; PS00062 ALDOKETO_REDUCTASE_2
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.0 %Cys (Translated Protein) 1.9 %Met (Translated Protein) 1.9 %Cys+Met (Translated Protein) 0.0 %Cys (Mature Protein) 1.5 %Met (Mature Protein) 1.5 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MSIPAFGLGTFRLQDQVVIDSVSTALELGYRAIDTAQIYENEAAVGQAIAASGVPRDELF CCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCHHEE ITTKIWIANLAKGKLIPSLRESLTKLQTSYVDLTLIHWPSPNDEVSVAEFMTELAEAKKL EEHHHHHHHHHCCCCCHHHHHHHHHHHHHHEEEEEEECCCCCCCCHHHHHHHHHHHHHHC GLTRQIGVSNFTLDLMQQAIDAVGSDQIATNQIELSPYLQNRKVVEFAQQHGIAITSYMT CCHHCCCCCHHHHHHHHHHHHHCCCCCEECCEEEECHHHHCCHHHHHHHHCCEEHHHHHH LAYGQALQDETIKAIAARRHATPAQVILAWALQLGYAVIPSSTKRENLASNLLAQQLTLT HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCEECCCCCHHHHHHHHHHHHHHHHH AEEMAEIAKLENNGRLVSPEGLAPNWD HHHHHHHHHHCCCCCEECCCCCCCCCC >Mature Secondary Structure SIPAFGLGTFRLQDQVVIDSVSTALELGYRAIDTAQIYENEAAVGQAIAASGVPRDELF CCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCHHEE ITTKIWIANLAKGKLIPSLRESLTKLQTSYVDLTLIHWPSPNDEVSVAEFMTELAEAKKL EEHHHHHHHHHCCCCCHHHHHHHHHHHHHHEEEEEEECCCCCCCCHHHHHHHHHHHHHHC GLTRQIGVSNFTLDLMQQAIDAVGSDQIATNQIELSPYLQNRKVVEFAQQHGIAITSYMT CCHHCCCCCHHHHHHHHHHHHHCCCCCEECCEEEECHHHHCCHHHHHHHHCCEEHHHHHH LAYGQALQDETIKAIAARRHATPAQVILAWALQLGYAVIPSSTKRENLASNLLAQQLTLT HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCEECCCCCHHHHHHHHHHHHHHHHH AEEMAEIAKLENNGRLVSPEGLAPNWD HHHHHHHHHHCCCCCEECCCCCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 10.0
TargetDB status: NA
Availability: NA
References: 11586360; 12142430 [H]