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The map label for this gene is rob [H]

Identifier: 157368842

GI number: 157368842

Start: 661544

End: 662422

Strand: Direct

Name: rob [H]

Synonym: Spro_0597

Alternate gene names: 157368842

Gene position: 661544-662422 (Clockwise)

Preceding gene: 157368840

Following gene: 157368843

Centisome position: 12.14

GC content: 58.02

Gene sequence:

>879_bases
ATGTATCAACAACAGGTCATTACGGCACTGCTGGCCTGGATCGAGCAGAACCTCGACCAGCCGCTGACGCTCGACGATAT
TGCGGCCAAATCAGGCTATTCCAAATGGCACCTGCAGCGGTTATTCAAAGAGTTAACCGGCGAAATCCTGGGAACTTACG
CCCGGCGCAGAAGGCTGACGGCCGCCGCACGCGAACTGCGGCTCACCAACCACGGCGTGGCCTTTATCGCCGACAAGTAT
CAGTTTGATTCCCAGCAGACTTTCACCCGCTGTTTCAAAAAGCAGTTTGGTATGCCGCCGGCCGGTTATCGCCGCAGTAC
CGACTGGTCAGGGCTGGGGATGCAGCCTCCGCTGCGGCTGATGGATGCGCCATTACCACAGGCGGAGATCATTACGCTGC
CGGCAATGCAATTGATTGGCCACACCCAGCACCGCAGTTGTACGTTGGGGCAATTGCTGCATTCGAAAAACGAGCTGCGT
CAGCATGCCTGGCGGCAGATGATGCCGAAGGAAGCCGAAACCTTGGCTGTGGTCTATGGCCTCACCAGTCTGGAGGCCGA
CAGCAGGCTACGCGGTAGGCAACGGATGGCTTATACCCTGGCGTTGGCGGACGAGCGGGCAGAAGGCGAACGGGTACTGA
TCGAGCAGGGCGACTACGCCAGCTTTACCTATCAGGGGCAGGTTGAAGAGTTACAAAACTTTATCGCCCGCCTGTATGAC
ACCGCGATACCCATGATGAAAGCAATACGGCGGCCGGGTAAGGATATCGAGCGTTTTTATCCCGCGTTGGGGGGCGTTTG
CTGCGCAGGCGGTACGGCGATCCGCTGCGAATATCTGATCCCTATCCGCCTGATGTCAGCGCCAATCGCCGCACTTTAG

Upstream 100 bases:

>100_bases
TAATTGAAAAACATTATGTCCTGATTGTGCAATGTGGTCTAGCACCGCGACCGATACAGTGAGCGCTGGTTTTGGCACTC
TCAGGAGATAAGGTGGCGCT

Downstream 100 bases:

>100_bases
CGGCGGTTTTCACAGTCACTCCTCTTCGTGCCGCACCAGGTTACAAAAAAGTTCGATATTGCGCCGGTAATGTTTCACAC
TTAGGTCGATGTGCACATAT

Product: AraC family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 292; Mature: 292

Protein sequence:

>292_residues
MYQQQVITALLAWIEQNLDQPLTLDDIAAKSGYSKWHLQRLFKELTGEILGTYARRRRLTAAARELRLTNHGVAFIADKY
QFDSQQTFTRCFKKQFGMPPAGYRRSTDWSGLGMQPPLRLMDAPLPQAEIITLPAMQLIGHTQHRSCTLGQLLHSKNELR
QHAWRQMMPKEAETLAVVYGLTSLEADSRLRGRQRMAYTLALADERAEGERVLIEQGDYASFTYQGQVEELQNFIARLYD
TAIPMMKAIRRPGKDIERFYPALGGVCCAGGTAIRCEYLIPIRLMSAPIAAL

Sequences:

>Translated_292_residues
MYQQQVITALLAWIEQNLDQPLTLDDIAAKSGYSKWHLQRLFKELTGEILGTYARRRRLTAAARELRLTNHGVAFIADKY
QFDSQQTFTRCFKKQFGMPPAGYRRSTDWSGLGMQPPLRLMDAPLPQAEIITLPAMQLIGHTQHRSCTLGQLLHSKNELR
QHAWRQMMPKEAETLAVVYGLTSLEADSRLRGRQRMAYTLALADERAEGERVLIEQGDYASFTYQGQVEELQNFIARLYD
TAIPMMKAIRRPGKDIERFYPALGGVCCAGGTAIRCEYLIPIRLMSAPIAAL
>Mature_292_residues
MYQQQVITALLAWIEQNLDQPLTLDDIAAKSGYSKWHLQRLFKELTGEILGTYARRRRLTAAARELRLTNHGVAFIADKY
QFDSQQTFTRCFKKQFGMPPAGYRRSTDWSGLGMQPPLRLMDAPLPQAEIITLPAMQLIGHTQHRSCTLGQLLHSKNELR
QHAWRQMMPKEAETLAVVYGLTSLEADSRLRGRQRMAYTLALADERAEGERVLIEQGDYASFTYQGQVEELQNFIARLYD
TAIPMMKAIRRPGKDIERFYPALGGVCCAGGTAIRCEYLIPIRLMSAPIAAL

Specific function: Binds to the right arm of the replication origin oriC of the chromosome. Rob binding may influence the formation of the nucleoprotein structure, required for oriC function in the initiation of replication [H]

COG id: COG2207

COG function: function code K; AraC-type DNA-binding domain-containing proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790857, Length=288, Percent_Identity=45.4861111111111, Blast_Score=254, Evalue=5e-69,
Organism=Escherichia coli, GI87081928, Length=99, Percent_Identity=49.4949494949495, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1790497, Length=104, Percent_Identity=50, Blast_Score=112, Evalue=4e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010499
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR020449
- InterPro:   IPR018060
- InterPro:   IPR011256 [H]

Pfam domain/function: PF06445 AraC_E_bind; PF00165 HTH_AraC [H]

EC number: NA

Molecular weight: Translated: 33153; Mature: 33153

Theoretical pI: Translated: 9.28; Mature: 9.28

Prosite motif: PS00041 HTH_ARAC_FAMILY_1 ; PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYQQQVITALLAWIEQNLDQPLTLDDIAAKSGYSKWHLQRLFKELTGEILGTYARRRRLT
CCHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAARELRLTNHGVAFIADKYQFDSQQTFTRCFKKQFGMPPAGYRRSTDWSGLGMQPPLRL
HHHHHHHHCCCCEEEEECCHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHH
MDAPLPQAEIITLPAMQLIGHTQHRSCTLGQLLHSKNELRQHAWRQMMPKEAETLAVVYG
HCCCCCCHHEEEHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHEEHHHH
LTSLEADSRLRGRQRMAYTLALADERAEGERVLIEQGDYASFTYQGQVEELQNFIARLYD
HHHCCHHHHHHHHHHHHHHEEECCCCCCCCEEEEECCCEEEEEECCCHHHHHHHHHHHHH
TAIPMMKAIRRPGKDIERFYPALGGVCCAGGTAIRCEYLIPIRLMSAPIAAL
HHHHHHHHHHCCCHHHHHHHHHHCCEECCCCCEEEEEEEEHHHHHCCCHHCC
>Mature Secondary Structure
MYQQQVITALLAWIEQNLDQPLTLDDIAAKSGYSKWHLQRLFKELTGEILGTYARRRRLT
CCHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAARELRLTNHGVAFIADKYQFDSQQTFTRCFKKQFGMPPAGYRRSTDWSGLGMQPPLRL
HHHHHHHHCCCCEEEEECCHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHH
MDAPLPQAEIITLPAMQLIGHTQHRSCTLGQLLHSKNELRQHAWRQMMPKEAETLAVVYG
HCCCCCCHHEEEHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHEEHHHH
LTSLEADSRLRGRQRMAYTLALADERAEGERVLIEQGDYASFTYQGQVEELQNFIARLYD
HHHCCHHHHHHHHHHHHHHEEECCCCCCCCEEEEECCCEEEEEECCCHHHHHHHHHHHHH
TAIPMMKAIRRPGKDIERFYPALGGVCCAGGTAIRCEYLIPIRLMSAPIAAL
HHHHHHHHHHCCCHHHHHHHHHHCCEECCCCCEEEEEEEEHHHHHCCCHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]