Click here to switch to the map view.

The map label for this gene is thiF [H]

Identifier: 157368531

GI number: 157368531

Start: 324304

End: 325053

Strand: Reverse

Name: thiF [H]

Synonym: Spro_0282

Alternate gene names: 157368531

Gene position: 325053-324304 (Counterclockwise)

Preceding gene: 157368532

Following gene: 157368530

Centisome position: 5.97

GC content: 60.4

Gene sequence:

>750_bases
ATGCTGAACGATCAAGAATTCCTGCGCTACAGCCGCCAGTTGCTGCTGGAAGACATCGGCCCCGCAGGCCAGGAGAAACT
AAAACAGTCGACGGCACTGATCGTCGGGTTGGGGGGGTTAGGCTCCCCCGCCTCGCTTTATCTGGCCGCCGCTGGCGTCG
GCACCCTGCTGCTGGCCGATGACGATAGGCTGCACGTCACCAATTTGCAGCGTCAGATCCTCTATCGCAGCAGCGACGTT
TCCCAAAGCAAAGCCGCCCTGGCAAAGCACCAGCTACAGGCGCTGAACCCGATGGTCGAATCCATTGCGCTGGAACAGCG
TCTGCAGGGGGAAGTGTTACAGCGCGCCGTACAACGCGCCGATTTAATTTTGGACTGTAGCGATAACATGGAAACCCGCC
ATGCGGTGAACGCCGCCTGCATCCAGGCCGGTAAACCGCTGATCAGCGGCAGTGCGGTGGGTTTCAGCGGTCAGCTGCTG
GTGATAGAACCTCCCTATGCCCACGGCTGCTACGCCTGCCTGTATCCGGAACAAACTGAACCCCAACGCAACTGCCGTAC
CGCTGGCGTACTCGGCCCGGTGGTCGGCGTCATTGGCACGCTGCAAGCGCTGGAAGCAATAAAAATGCTGGCGGGCCTGC
CTTCCGCCATCAGCGGCAAACTGCGGCTGTTCGACGGTAAACAACAAAGCTGGAGCACCTTGCAACTCAGTCAGGCCAGC
ACCTGTCCGGCGTGCGGAGGGACAGCATGA

Upstream 100 bases:

>100_bases
GTCGGTAGCGTCGCCGTGGTCAGCGCCATTACCCAGGCCCCGGACTGGCGCGCAGCGACTGCACAGCTGCTGCAACTGAT
CGAAGGCAAGGAGTGATGCC

Downstream 100 bases:

>100_bases
AAATTCGGCTTAATGACCAGCCGCTGGAGCTGGCACAACCGCTGAGCATCAGCGCCCTGCTCGAGCAACTGGAACGCCAC
CAGCCGGGCACGGCGCTGGC

Product: UBA/THIF-type NAD/FAD binding protein

Products: ThiS-COSH; AMP; ThiS-COAMP; pyrophosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 249; Mature: 249

Protein sequence:

>249_residues
MLNDQEFLRYSRQLLLEDIGPAGQEKLKQSTALIVGLGGLGSPASLYLAAAGVGTLLLADDDRLHVTNLQRQILYRSSDV
SQSKAALAKHQLQALNPMVESIALEQRLQGEVLQRAVQRADLILDCSDNMETRHAVNAACIQAGKPLISGSAVGFSGQLL
VIEPPYAHGCYACLYPEQTEPQRNCRTAGVLGPVVGVIGTLQALEAIKMLAGLPSAISGKLRLFDGKQQSWSTLQLSQAS
TCPACGGTA

Sequences:

>Translated_249_residues
MLNDQEFLRYSRQLLLEDIGPAGQEKLKQSTALIVGLGGLGSPASLYLAAAGVGTLLLADDDRLHVTNLQRQILYRSSDV
SQSKAALAKHQLQALNPMVESIALEQRLQGEVLQRAVQRADLILDCSDNMETRHAVNAACIQAGKPLISGSAVGFSGQLL
VIEPPYAHGCYACLYPEQTEPQRNCRTAGVLGPVVGVIGTLQALEAIKMLAGLPSAISGKLRLFDGKQQSWSTLQLSQAS
TCPACGGTA
>Mature_249_residues
MLNDQEFLRYSRQLLLEDIGPAGQEKLKQSTALIVGLGGLGSPASLYLAAAGVGTLLLADDDRLHVTNLQRQILYRSSDV
SQSKAALAKHQLQALNPMVESIALEQRLQGEVLQRAVQRADLILDCSDNMETRHAVNAACIQAGKPLISGSAVGFSGQLL
VIEPPYAHGCYACLYPEQTEPQRNCRTAGVLGPVVGVIGTLQALEAIKMLAGLPSAISGKLRLFDGKQQSWSTLQLSQAS
TCPACGGTA

Specific function: Catalyzes the adenylation by ATP of the carboxyl group of the C-terminal glycine of sulfur carrier protein ThiS [H]

COG id: COG0476

COG function: function code H; Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hesA/moeB/thiF family [H]

Homologues:

Organism=Homo sapiens, GI7657339, Length=249, Percent_Identity=45.3815261044177, Blast_Score=166, Evalue=2e-41,
Organism=Homo sapiens, GI4885649, Length=153, Percent_Identity=30.0653594771242, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI38045948, Length=165, Percent_Identity=31.5151515151515, Blast_Score=67, Evalue=1e-11,
Organism=Escherichia coli, GI87082356, Length=247, Percent_Identity=59.5141700404858, Blast_Score=298, Evalue=2e-82,
Organism=Escherichia coli, GI1787048, Length=245, Percent_Identity=43.265306122449, Blast_Score=196, Evalue=1e-51,
Organism=Caenorhabditis elegans, GI17540406, Length=245, Percent_Identity=41.6326530612245, Blast_Score=187, Evalue=5e-48,
Organism=Caenorhabditis elegans, GI193203301, Length=152, Percent_Identity=32.8947368421053, Blast_Score=85, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI71984476, Length=176, Percent_Identity=31.25, Blast_Score=71, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17508819, Length=225, Percent_Identity=26.2222222222222, Blast_Score=64, Evalue=7e-11,
Organism=Saccharomyces cerevisiae, GI6321903, Length=249, Percent_Identity=36.144578313253, Blast_Score=157, Evalue=1e-39,
Organism=Saccharomyces cerevisiae, GI6320598, Length=168, Percent_Identity=29.1666666666667, Blast_Score=69, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6322639, Length=170, Percent_Identity=27.0588235294118, Blast_Score=67, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24582879, Length=248, Percent_Identity=43.5483870967742, Blast_Score=192, Evalue=2e-49,
Organism=Drosophila melanogaster, GI24660640, Length=154, Percent_Identity=31.1688311688312, Blast_Score=81, Evalue=8e-16,
Organism=Drosophila melanogaster, GI28573937, Length=183, Percent_Identity=29.5081967213115, Blast_Score=69, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012731
- InterPro:   IPR007901
- InterPro:   IPR009036
- InterPro:   IPR016040
- InterPro:   IPR000594 [H]

Pfam domain/function: PF05237 MoeZ_MoeB; PF00899 ThiF [H]

EC number: 2.7.7.- [C]

Molecular weight: Translated: 26435; Mature: 26435

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNDQEFLRYSRQLLLEDIGPAGQEKLKQSTALIVGLGGLGSPASLYLAAAGVGTLLLAD
CCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHEEEEEECCCCCCHHHHHHHCCCCEEEEEC
DDRLHVTNLQRQILYRSSDVSQSKAALAKHQLQALNPMVESIALEQRLQGEVLQRAVQRA
CCCEEHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DLILDCSDNMETRHAVNAACIQAGKPLISGSAVGFSGQLLVIEPPYAHGCYACLYPEQTE
CEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCEEEEECCCCCC
PQRNCRTAGVLGPVVGVIGTLQALEAIKMLAGLPSAISGKLRLFDGKQQSWSTLQLSQAS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCEEEECCCCCCCCEEEECCCC
TCPACGGTA
CCCCCCCCC
>Mature Secondary Structure
MLNDQEFLRYSRQLLLEDIGPAGQEKLKQSTALIVGLGGLGSPASLYLAAAGVGTLLLAD
CCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHEEEEEECCCCCCHHHHHHHCCCCEEEEEC
DDRLHVTNLQRQILYRSSDVSQSKAALAKHQLQALNPMVESIALEQRLQGEVLQRAVQRA
CCCEEHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DLILDCSDNMETRHAVNAACIQAGKPLISGSAVGFSGQLLVIEPPYAHGCYACLYPEQTE
CEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCEEEEECCCCCC
PQRNCRTAGVLGPVVGVIGTLQALEAIKMLAGLPSAISGKLRLFDGKQQSWSTLQLSQAS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCEEEECCCCCCCCEEEECCCC
TCPACGGTA
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ThiS-COAMP; L-cysteine; ThiS protein; ATP [C]

Specific reaction: ThiS-COAMP + L-cysteine = ThiS-COSH + AMP ThiS protein + ATP = ThiS-COAMP + pyrophosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8432721; 8265357; 9278503; 9632726; 10082377 [H]