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The map label for this gene is thiE

Identifier: 157368532

GI number: 157368532

Start: 325058

End: 325696

Strand: Reverse

Name: thiE

Synonym: Spro_0283

Alternate gene names: 157368532

Gene position: 325696-325058 (Counterclockwise)

Preceding gene: 157368533

Following gene: 157368531

Centisome position: 5.98

GC content: 58.84

Gene sequence:

>639_bases
ATGACTGATATCACCACGCCCTTCCCCGCCACGCCTCACAAGTTGGGGTTATACCCGGTGGTCGACAGCGTTGAATGGAT
TGCCCGTTTGCTGGACGCAGGCGTCACTACCCTTCAACTGCGGATTAAGGATCTGCCGGATGAACAGGTCGAGGACGACA
TTGCCGCCGCTATTGCCCTCGGCAAGCGCTACGACGCCCGATTGTTTATCAACGACTACTGGCAACTGGCGATTAAGCAC
GGTGCCTATGGCGTACATCTCGGCCAGGAGGATTTGGATACTACCGATCTGGCCGCCATTCACCGCGCCGGGCTACGTTT
GGGGGTTTCCACCCATGACGATAGTGAACTGGCCCGCGCCATTGCAGTAAAACCTTCCTATATCGCACTGGGGCATATTT
TTCCGACGCAAACCAAAGACATGCCTTCTGCCCCACAGGGTCTGGTGGAGCTGAAACGCCATATCGCCGGGCTGAGTGAC
TACCCAACGGTGGCGATCGGCGGGATCAGTATCGATCGCGTGGCGGCAGTGCTGGATTGTGGCGTCGGTAGCGTCGCCGT
GGTCAGCGCCATTACCCAGGCCCCGGACTGGCGCGCAGCGACTGCACAGCTGCTGCAACTGATCGAAGGCAAGGAGTGA

Upstream 100 bases:

>100_bases
GCAGTGCAGTTAACCGGCATGGAAAAGATGTCGGCCGAGTTCCGCTCACGCGGCAGCGAGCTGTACCACAGCGCCGGTAA
CCTGCAAGAGGAATTAAACA

Downstream 100 bases:

>100_bases
TGCCATGCTGAACGATCAAGAATTCCTGCGCTACAGCCGCCAGTTGCTGCTGGAAGACATCGGCCCCGCAGGCCAGGAGA
AACTAAAACAGTCGACGGCA

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase

Number of amino acids: Translated: 212; Mature: 211

Protein sequence:

>212_residues
MTDITTPFPATPHKLGLYPVVDSVEWIARLLDAGVTTLQLRIKDLPDEQVEDDIAAAIALGKRYDARLFINDYWQLAIKH
GAYGVHLGQEDLDTTDLAAIHRAGLRLGVSTHDDSELARAIAVKPSYIALGHIFPTQTKDMPSAPQGLVELKRHIAGLSD
YPTVAIGGISIDRVAAVLDCGVGSVAVVSAITQAPDWRAATAQLLQLIEGKE

Sequences:

>Translated_212_residues
MTDITTPFPATPHKLGLYPVVDSVEWIARLLDAGVTTLQLRIKDLPDEQVEDDIAAAIALGKRYDARLFINDYWQLAIKH
GAYGVHLGQEDLDTTDLAAIHRAGLRLGVSTHDDSELARAIAVKPSYIALGHIFPTQTKDMPSAPQGLVELKRHIAGLSD
YPTVAIGGISIDRVAAVLDCGVGSVAVVSAITQAPDWRAATAQLLQLIEGKE
>Mature_211_residues
TDITTPFPATPHKLGLYPVVDSVEWIARLLDAGVTTLQLRIKDLPDEQVEDDIAAAIALGKRYDARLFINDYWQLAIKHG
AYGVHLGQEDLDTTDLAAIHRAGLRLGVSTHDDSELARAIAVKPSYIALGHIFPTQTKDMPSAPQGLVELKRHIAGLSDY
PTVAIGGISIDRVAAVLDCGVGSVAVVSAITQAPDWRAATAQLLQLIEGKE

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP)

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family

Homologues:

Organism=Escherichia coli, GI1790426, Length=200, Percent_Identity=74.5, Blast_Score=303, Evalue=7e-84,
Organism=Saccharomyces cerevisiae, GI6325042, Length=199, Percent_Identity=31.1557788944724, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIE_SERP5 (A8G8F3)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001476521.1
- ProteinModelPortal:   A8G8F3
- STRING:   A8G8F3
- GeneID:   5607050
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_0283
- eggNOG:   COG0352
- HOGENOM:   HBG754477
- OMA:   HAVQPSY
- ProtClustDB:   PRK03512
- BioCyc:   SPRO399741:SPRO_0283-MONOMER
- HAMAP:   MF_00097
- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00693

Pfam domain/function: PF02581 TMP-TENI; SSF51391 TMP_synthase

EC number: =2.5.1.3

Molecular weight: Translated: 22793; Mature: 22662

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDITTPFPATPHKLGLYPVVDSVEWIARLLDAGVTTLQLRIKDLPDEQVEDDIAAAIAL
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHH
GKRYDARLFINDYWQLAIKHGAYGVHLGQEDLDTTDLAAIHRAGLRLGVSTHDDSELARA
CCCCCCEEEECHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHEECCCCCCHHHHHHH
IAVKPSYIALGHIFPTQTKDMPSAPQGLVELKRHIAGLSDYPTVAIGGISIDRVAAVLDC
HHCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHC
GVGSVAVVSAITQAPDWRAATAQLLQLIEGKE
CCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TDITTPFPATPHKLGLYPVVDSVEWIARLLDAGVTTLQLRIKDLPDEQVEDDIAAAIAL
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHH
GKRYDARLFINDYWQLAIKHGAYGVHLGQEDLDTTDLAAIHRAGLRLGVSTHDDSELARA
CCCCCCEEEECHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHEECCCCCCHHHHHHH
IAVKPSYIALGHIFPTQTKDMPSAPQGLVELKRHIAGLSDYPTVAIGGISIDRVAAVLDC
HHCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHC
GVGSVAVVSAITQAPDWRAATAQLLQLIEGKE
CCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA