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The map label for this gene is thiS [H]

Identifier: 157368530

GI number: 157368530

Start: 324107

End: 324307

Strand: Reverse

Name: thiS [H]

Synonym: Spro_0281

Alternate gene names: 157368530

Gene position: 324307-324107 (Counterclockwise)

Preceding gene: 157368531

Following gene: 157368529

Centisome position: 5.95

GC content: 59.7

Gene sequence:

>201_bases
ATGAAAATTCGGCTTAATGACCAGCCGCTGGAGCTGGCACAACCGCTGAGCATCAGCGCCCTGCTCGAGCAACTGGAACG
CCACCAGCCGGGCACGGCGCTGGCTATCAACCAAATCATCATCCCGCGCACCGACTGGGCTAACCACCTGGTGCAAGACG
GCGATGACATTCTGCTGTTTCAAGCGATCGCCGGAGGCTGA

Upstream 100 bases:

>100_bases
CCATCAGCGGCAAACTGCGGCTGTTCGACGGTAAACAACAAAGCTGGAGCACCTTGCAACTCAGTCAGGCCAGCACCTGT
CCGGCGTGCGGAGGGACAGC

Downstream 100 bases:

>100_bases
CATGCTACAAATCGCCGATACCACTTTTACCTCACGCCTGTTTACCGGCACCGGTAAATTCGCCACCCCAACGCTGATGC
TGGAGGCATTACAGGCCTCC

Product: sulfur carrier protein ThiS

Products: NA

Alternate protein names: Thiamine biosynthesis protein ThiS [H]

Number of amino acids: Translated: 66; Mature: 66

Protein sequence:

>66_residues
MKIRLNDQPLELAQPLSISALLEQLERHQPGTALAINQIIIPRTDWANHLVQDGDDILLFQAIAGG

Sequences:

>Translated_66_residues
MKIRLNDQPLELAQPLSISALLEQLERHQPGTALAINQIIIPRTDWANHLVQDGDDILLFQAIAGG
>Mature_66_residues
MKIRLNDQPLELAQPLSISALLEQLERHQPGTALAINQIIIPRTDWANHLVQDGDDILLFQAIAGG

Specific function: Is the sulfur donor in the synthesis of the thiazole phosphate moiety of thiamine phosphate [H]

COG id: COG2104

COG function: function code H; Sulfur transfer protein involved in thiamine biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sulfur carrier protein ThiS family [H]

Homologues:

Organism=Escherichia coli, GI48994994, Length=66, Percent_Identity=59.0909090909091, Blast_Score=82, Evalue=8e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012675
- InterPro:   IPR016155
- InterPro:   IPR010035
- InterPro:   IPR003749 [H]

Pfam domain/function: PF02597 ThiS [H]

EC number: NA

Molecular weight: Translated: 7296; Mature: 7296

Theoretical pI: Translated: 4.49; Mature: 4.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIRLNDQPLELAQPLSISALLEQLERHQPGTALAINQIIIPRTDWANHLVQDGDDILLF
CEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEECEEEECCCCHHHHHHCCCCCEEEE
QAIAGG
EEECCC
>Mature Secondary Structure
MKIRLNDQPLELAQPLSISALLEQLERHQPGTALAINQIIIPRTDWANHLVQDGDDILLF
CEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEECEEEECCCCHHHHHHCCCCCEEEE
QAIAGG
EEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8432721; 8265357; 9278503; 9632726; 10082377 [H]