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The map label for this gene is rffG [H]

Identifier: 157368414

GI number: 157368414

Start: 186792

End: 187859

Strand: Direct

Name: rffG [H]

Synonym: Spro_0165

Alternate gene names: 157368414

Gene position: 186792-187859 (Clockwise)

Preceding gene: 157368413

Following gene: 157368415

Centisome position: 3.43

GC content: 56.09

Gene sequence:

>1068_bases
TTGAAACGTATTTTAGTCACTGGAGGCGCCGGCTTTATTGGCTCCGCAGTCGTGAGGCATATTATCGAAGCCACGCAGGA
CAGTGTCCTGGTGCTGGATAAGCTCACCTACGCGGGCAATCTGGAGTCGCTTTCGGTGGTTGCCGATAATCCTCGTTATT
CGTTTGAACAGGTGGATATCTGCGATCGCGCGGCGTTGGATCGGGTGTTTGCCGAGTACCAGCCGGACGTGATCATGCAT
CTGGCGGCAGAAAGCCACGTTGACCGTTCTATCGACGGCCCGGCGGCATTTATTGAAACCAACGTGGTGGGCACCTACAC
CATGCTGGAGGCGGCCCGCCATTATTGGCAGCCGCTGGACGCGGCAAAGAAGCAAAGCTTCCGTTTCCACCACATTTCCA
CCGACGAAGTGTATGGTGATCTGCACGGTACGGACGATCTGTTTACCGAAACCACGCCTTACTCGCCAAGCAGCCCGTAT
TCGGCCTCGAAGGCCTCCAGCGATCACCTGGTCCGCGCCTGGCTGCGTACTTATGGCCTGCCGACTCTGGTCACCAACTG
TTCCAATAACTACGGTCCCTATCACTTCCCGGAGAAACTGATCCCGTTGGTGATCCTCAATGCCGTTGCCGGTAAACCCT
TGCCGGTGTATGGCGATGGGGCGCAGGTGCGTGATTGGCTGTATGTCGAAGACCACGCTCGCGCGCTGTACCAGGTGGTT
ACCGAAGGCGTGGTAGGGGAAACCTACAATATTGGCGGCCACAATGAGCGCAAGAATATTGAAGTGGTGCAGACCATCTG
CGACCTGCTTGAAGAGTTGGCGCCGAACAAACCACAGGGCGTGGAGAAATACCGCGACCTGATTACCTACGTCAAGGATC
GTCCGGGCCATGATATGCGCTACGCGATCGATGCCGGAAAGATTGACAGGGAGCTGGGCTGGCGGCCGCAGGAAACCTTT
GAAAGCGGCATCCGCAAAACGGTTTCCTGGTATCTGAACAATGAAATCTGGTGGCGTCGCGTTCAGGACGGTTCCTATGC
GGGTGAACGATTAGGTTTATCCGACTAA

Upstream 100 bases:

>100_bases
CGGATGTGATCGTGATGCTGGTCGATCATAAGCAATTCAAGGCGATCAAGCCGGAAGAAATTACGCAGTCCTGGATCGTG
GATACCAAAGGGGTTTGGCG

Downstream 100 bases:

>100_bases
ATTTAGCGAGGCCACCGCTTAAGCGGTAGCCCTGTGGATTCTGGAGTGAGTATGAAAGGTATTATTCTGGCGGGCGGTTC
CGGCACACGCTTGCATCCGA

Product: dTDP-glucose 4,6-dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 355; Mature: 355

Protein sequence:

>355_residues
MKRILVTGGAGFIGSAVVRHIIEATQDSVLVLDKLTYAGNLESLSVVADNPRYSFEQVDICDRAALDRVFAEYQPDVIMH
LAAESHVDRSIDGPAAFIETNVVGTYTMLEAARHYWQPLDAAKKQSFRFHHISTDEVYGDLHGTDDLFTETTPYSPSSPY
SASKASSDHLVRAWLRTYGLPTLVTNCSNNYGPYHFPEKLIPLVILNAVAGKPLPVYGDGAQVRDWLYVEDHARALYQVV
TEGVVGETYNIGGHNERKNIEVVQTICDLLEELAPNKPQGVEKYRDLITYVKDRPGHDMRYAIDAGKIDRELGWRPQETF
ESGIRKTVSWYLNNEIWWRRVQDGSYAGERLGLSD

Sequences:

>Translated_355_residues
MKRILVTGGAGFIGSAVVRHIIEATQDSVLVLDKLTYAGNLESLSVVADNPRYSFEQVDICDRAALDRVFAEYQPDVIMH
LAAESHVDRSIDGPAAFIETNVVGTYTMLEAARHYWQPLDAAKKQSFRFHHISTDEVYGDLHGTDDLFTETTPYSPSSPY
SASKASSDHLVRAWLRTYGLPTLVTNCSNNYGPYHFPEKLIPLVILNAVAGKPLPVYGDGAQVRDWLYVEDHARALYQVV
TEGVVGETYNIGGHNERKNIEVVQTICDLLEELAPNKPQGVEKYRDLITYVKDRPGHDMRYAIDAGKIDRELGWRPQETF
ESGIRKTVSWYLNNEIWWRRVQDGSYAGERLGLSD
>Mature_355_residues
MKRILVTGGAGFIGSAVVRHIIEATQDSVLVLDKLTYAGNLESLSVVADNPRYSFEQVDICDRAALDRVFAEYQPDVIMH
LAAESHVDRSIDGPAAFIETNVVGTYTMLEAARHYWQPLDAAKKQSFRFHHISTDEVYGDLHGTDDLFTETTPYSPSSPY
SASKASSDHLVRAWLRTYGLPTLVTNCSNNYGPYHFPEKLIPLVILNAVAGKPLPVYGDGAQVRDWLYVEDHARALYQVV
TEGVVGETYNIGGHNERKNIEVVQTICDLLEELAPNKPQGVEKYRDLITYVKDRPGHDMRYAIDAGKIDRELGWRPQETF
ESGIRKTVSWYLNNEIWWRRVQDGSYAGERLGLSD

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction [H]

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=344, Percent_Identity=35.7558139534884, Blast_Score=227, Evalue=1e-59,
Organism=Homo sapiens, GI42516563, Length=336, Percent_Identity=26.4880952380952, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI56237023, Length=295, Percent_Identity=29.8305084745763, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI56118217, Length=295, Percent_Identity=29.8305084745763, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI189083684, Length=295, Percent_Identity=29.8305084745763, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI4504031, Length=346, Percent_Identity=24.2774566473988, Blast_Score=69, Evalue=5e-12,
Organism=Escherichia coli, GI48994969, Length=353, Percent_Identity=80.1699716713881, Blast_Score=609, Evalue=1e-176,
Organism=Escherichia coli, GI1788353, Length=352, Percent_Identity=73.8636363636364, Blast_Score=537, Evalue=1e-154,
Organism=Escherichia coli, GI1786974, Length=353, Percent_Identity=26.628895184136, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1788366, Length=372, Percent_Identity=25.8064516129032, Blast_Score=81, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17568069, Length=348, Percent_Identity=35.3448275862069, Blast_Score=207, Evalue=5e-54,
Organism=Caenorhabditis elegans, GI115532424, Length=335, Percent_Identity=32.8358208955224, Blast_Score=172, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI71982035, Length=365, Percent_Identity=26.8493150684932, Blast_Score=96, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI71982038, Length=367, Percent_Identity=26.7029972752044, Blast_Score=94, Evalue=9e-20,
Organism=Caenorhabditis elegans, GI17539532, Length=339, Percent_Identity=25.9587020648968, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI133901786, Length=296, Percent_Identity=24.6621621621622, Blast_Score=72, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI133901788, Length=296, Percent_Identity=24.6621621621622, Blast_Score=72, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17539422, Length=296, Percent_Identity=24.6621621621622, Blast_Score=71, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI133901790, Length=296, Percent_Identity=24.6621621621622, Blast_Score=71, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17539424, Length=296, Percent_Identity=24.6621621621622, Blast_Score=71, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI17507723, Length=295, Percent_Identity=24.0677966101695, Blast_Score=71, Evalue=8e-13,
Organism=Saccharomyces cerevisiae, GI6319493, Length=369, Percent_Identity=25.4742547425474, Blast_Score=96, Evalue=1e-20,
Organism=Drosophila melanogaster, GI19923002, Length=365, Percent_Identity=27.1232876712329, Blast_Score=92, Evalue=8e-19,
Organism=Drosophila melanogaster, GI21356223, Length=349, Percent_Identity=25.7879656160458, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24158427, Length=277, Percent_Identity=25.9927797833935, Blast_Score=73, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 39940; Mature: 39940

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRILVTGGAGFIGSAVVRHIIEATQDSVLVLDKLTYAGNLESLSVVADNPRYSFEQVDI
CCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEHHHHCCCCCEEEEEECCCCCCCHHHHH
CDRAALDRVFAEYQPDVIMHLAAESHVDRSIDGPAAFIETNVVGTYTMLEAARHYWQPLD
HHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCHHEEECCCCHHHHHHHHHHHHHCCHH
AAKKQSFRFHHISTDEVYGDLHGTDDLFTETTPYSPSSPYSASKASSDHLVRAWLRTYGL
HHHHCCEEEEECCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCC
PTLVTNCSNNYGPYHFPEKLIPLVILNAVAGKPLPVYGDGAQVRDWLYVEDHARALYQVV
HHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEECCCCCHHHEEEEHHHHHHHHHHH
TEGVVGETYNIGGHNERKNIEVVQTICDLLEELAPNKPQGVEKYRDLITYVKDRPGHDMR
HHCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCEE
YAIDAGKIDRELGWRPQETFESGIRKTVSWYLNNEIWWRRVQDGSYAGERLGLSD
EEECCCCHHHHHCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCC
>Mature Secondary Structure
MKRILVTGGAGFIGSAVVRHIIEATQDSVLVLDKLTYAGNLESLSVVADNPRYSFEQVDI
CCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEHHHHCCCCCEEEEEECCCCCCCHHHHH
CDRAALDRVFAEYQPDVIMHLAAESHVDRSIDGPAAFIETNVVGTYTMLEAARHYWQPLD
HHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCHHEEECCCCHHHHHHHHHHHHHCCHH
AAKKQSFRFHHISTDEVYGDLHGTDDLFTETTPYSPSSPYSASKASSDHLVRAWLRTYGL
HHHHCCEEEEECCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCC
PTLVTNCSNNYGPYHFPEKLIPLVILNAVAGKPLPVYGDGAQVRDWLYVEDHARALYQVV
HHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEECCCCCHHHEEEEHHHHHHHHHHH
TEGVVGETYNIGGHNERKNIEVVQTICDLLEELAPNKPQGVEKYRDLITYVKDRPGHDMR
HHCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCEE
YAIDAGKIDRELGWRPQETFESGIRKTVSWYLNNEIWWRRVQDGSYAGERLGLSD
EEECCCCHHHHHCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1379743; 9278503; 7559340 [H]