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The map label for this gene is cmr [H]

Identifier: 157368341

GI number: 157368341

Start: 97654

End: 98901

Strand: Reverse

Name: cmr [H]

Synonym: Spro_0092

Alternate gene names: 157368341

Gene position: 98901-97654 (Counterclockwise)

Preceding gene: 157368343

Following gene: 157368331

Centisome position: 1.82

GC content: 60.26

Gene sequence:

>1248_bases
ATGAAAACAACATTACCACCTGCGGCTCGCTTGGGTCGACAGGCGCTTTTATTCCCTCTTTGTCTGGTGCTGTTCGAGTT
CGCCACCTATATCGCCAACGATATGATCCAGCCTGGCATGCTGGCGGTGGTGGCAGATTTCAACGCCGGAGAAGAGTGGG
TGCCAACCTCAATGACCGCCTATCTGGCCGGCGGTATTTTCCTGCAGTGGCTGCTGGGGCCATTGTCGGATCGCCGTGGT
CGTCGCCCGGTGATGCTGGCGGGTGTGGCGTTCTTTATCGTCGCCTGCCTGGCGATTTTGCTGGTGACCAGCATCGAGCA
ATTTATCTTTATGCGTTTCCTGCAGGGGATTGGCCTGTGCTTTATCGGCGCGGTCGGCTACGCCACGATACAGGAATCTT
TTGAGGAATCTGTGTGCATAAAAATCACCGCACTGATGGCCAACGTGGCGCTGATTGCCCCGTTGCTCGGGCCATTGGCC
GGTGCTGCGCTGATCCACGTCGCGCCGTGGCAGAGCATGTTCGTGCTGTTCGCGGCGCTGGCGGCCTTTGCCTTCTATGG
CCTGTGGAAAGCGATGCCGGAAACCGCGTCGCTGCAGGGTGAGAAACTCTCGGCCGCCAACCTGTGGCGTGACTATCGCC
AGGTGCTGTCCAACCGTCGTTTTGTCTGCGGGGCGCTGGCCATTGGCTTCGCCAGCCTGCCGCTGCTGGCCTGGATTGCC
CAATCACCGGTGATCCTGATCAGCGGCGAGTCGCTGTCGACGCTCGATTACGGCCTGCTGCAAATTCCGGTGTTCGGTGC
GCTGATCCTCGGCAACCTGACGCTGGCGCGTTTGACCGGTAAAAACAGCGTACAACGCCTGATCAAACTGGGTGCAGGGC
CAATGATGCTGGGGCTGCTGATCGCGGCGCTGGCTACCCTGTTCTCGACCCACGCCTACCTGTGGATGACCGCAGGCCTG
AGCCTGTACGCCTACGGTATCGGGCTGGCCAACGCCGGGCTGTACCGCCTGACGCTGTTCTCAAGCTCCGTCAGCAAAGG
CACGGTTTCCGCCACCATGGGCATGTTGAGCATGATGGTATTCACCGTCGGCATAGAACTGGCGAAAGTGGCCTACGTGT
GGGGCGGCAGCGGGTTGTTCAATCTGTTCAACCTGATTAGCGGCCTGTGCTGGCTGGCGCTGGTAGCGCTGTTCCTCAGC
AAACGCCGCAGCGGCGACGCAACGCCGACGCCTAACGCCGCATTGTAA

Upstream 100 bases:

>100_bases
AGGTGAACAATTGGCGGCAAAATAGCCGCAACAACTCGCTCAGATAGCGTTCCCCGCCCTGACAGTTCCTTATTTATTCT
TTACTTTCAGTACGATAACT

Downstream 100 bases:

>100_bases
TGATCAGCACGTGAGGAGAGGTTATCTCCTCACGTGCTGTAAAGTTTGCATTGGATTCAACACCCTCAACTCCGTTTGTC
AGCATCCAAATTTATCGCCC

Product: major facilitator transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Chloramphenicol resistance pump cmr [H]

Number of amino acids: Translated: 415; Mature: 415

Protein sequence:

>415_residues
MKTTLPPAARLGRQALLFPLCLVLFEFATYIANDMIQPGMLAVVADFNAGEEWVPTSMTAYLAGGIFLQWLLGPLSDRRG
RRPVMLAGVAFFIVACLAILLVTSIEQFIFMRFLQGIGLCFIGAVGYATIQESFEESVCIKITALMANVALIAPLLGPLA
GAALIHVAPWQSMFVLFAALAAFAFYGLWKAMPETASLQGEKLSAANLWRDYRQVLSNRRFVCGALAIGFASLPLLAWIA
QSPVILISGESLSTLDYGLLQIPVFGALILGNLTLARLTGKNSVQRLIKLGAGPMMLGLLIAALATLFSTHAYLWMTAGL
SLYAYGIGLANAGLYRLTLFSSSVSKGTVSATMGMLSMMVFTVGIELAKVAYVWGGSGLFNLFNLISGLCWLALVALFLS
KRRSGDATPTPNAAL

Sequences:

>Translated_415_residues
MKTTLPPAARLGRQALLFPLCLVLFEFATYIANDMIQPGMLAVVADFNAGEEWVPTSMTAYLAGGIFLQWLLGPLSDRRG
RRPVMLAGVAFFIVACLAILLVTSIEQFIFMRFLQGIGLCFIGAVGYATIQESFEESVCIKITALMANVALIAPLLGPLA
GAALIHVAPWQSMFVLFAALAAFAFYGLWKAMPETASLQGEKLSAANLWRDYRQVLSNRRFVCGALAIGFASLPLLAWIA
QSPVILISGESLSTLDYGLLQIPVFGALILGNLTLARLTGKNSVQRLIKLGAGPMMLGLLIAALATLFSTHAYLWMTAGL
SLYAYGIGLANAGLYRLTLFSSSVSKGTVSATMGMLSMMVFTVGIELAKVAYVWGGSGLFNLFNLISGLCWLALVALFLS
KRRSGDATPTPNAAL
>Mature_415_residues
MKTTLPPAARLGRQALLFPLCLVLFEFATYIANDMIQPGMLAVVADFNAGEEWVPTSMTAYLAGGIFLQWLLGPLSDRRG
RRPVMLAGVAFFIVACLAILLVTSIEQFIFMRFLQGIGLCFIGAVGYATIQESFEESVCIKITALMANVALIAPLLGPLA
GAALIHVAPWQSMFVLFAALAAFAFYGLWKAMPETASLQGEKLSAANLWRDYRQVLSNRRFVCGALAIGFASLPLLAWIA
QSPVILISGESLSTLDYGLLQIPVFGALILGNLTLARLTGKNSVQRLIKLGAGPMMLGLLIAALATLFSTHAYLWMTAGL
SLYAYGIGLANAGLYRLTLFSSSVSKGTVSATMGMLSMMVFTVGIELAKVAYVWGGSGLFNLFNLISGLCWLALVALFLS
KRRSGDATPTPNAAL

Specific function: Efflux pump driven by the proton motive force. Confers resistance to basic, aromatic and lipophilic drugs as well as neutral, non-aromatic, zwitterionic or non-aromatic hydrophilic antibiotics [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily [H]

Homologues:

Organism=Escherichia coli, GI1787065, Length=407, Percent_Identity=72.2358722358722, Blast_Score=596, Evalue=1e-171,
Organism=Escherichia coli, GI1790794, Length=392, Percent_Identity=42.6020408163265, Blast_Score=330, Evalue=7e-92,
Organism=Escherichia coli, GI48994889, Length=242, Percent_Identity=27.2727272727273, Blast_Score=88, Evalue=9e-19,
Organism=Escherichia coli, GI1790146, Length=217, Percent_Identity=28.5714285714286, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI87082312, Length=193, Percent_Identity=27.979274611399, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1788509, Length=251, Percent_Identity=23.9043824701195, Blast_Score=66, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005829 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 44404; Mature: 44404

Theoretical pI: Translated: 9.34; Mature: 9.34

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTTLPPAARLGRQALLFPLCLVLFEFATYIANDMIQPGMLAVVADFNAGEEWVPTSMTA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCHHHHH
YLAGGIFLQWLLGPLSDRRGRRPVMLAGVAFFIVACLAILLVTSIEQFIFMRFLQGIGLC
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FIGAVGYATIQESFEESVCIKITALMANVALIAPLLGPLAGAALIHVAPWQSMFVLFAAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCHHHHHHHHHHH
AAFAFYGLWKAMPETASLQGEKLSAANLWRDYRQVLSNRRFVCGALAIGFASLPLLAWIA
HHHHHHHHHHHCCCHHCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
QSPVILISGESLSTLDYGLLQIPVFGALILGNLTLARLTGKNSVQRLIKLGAGPMMLGLL
CCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCHHHHHHCCHHHHHHHHHHCCCHHHHHHH
IAALATLFSTHAYLWMTAGLSLYAYGIGLANAGLYRLTLFSSSVSKGTVSATMGMLSMMV
HHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHH
FTVGIELAKVAYVWGGSGLFNLFNLISGLCWLALVALFLSKRRSGDATPTPNAAL
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MKTTLPPAARLGRQALLFPLCLVLFEFATYIANDMIQPGMLAVVADFNAGEEWVPTSMTA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCHHHHH
YLAGGIFLQWLLGPLSDRRGRRPVMLAGVAFFIVACLAILLVTSIEQFIFMRFLQGIGLC
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FIGAVGYATIQESFEESVCIKITALMANVALIAPLLGPLAGAALIHVAPWQSMFVLFAAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCHHHHHHHHHHH
AAFAFYGLWKAMPETASLQGEKLSAANLWRDYRQVLSNRRFVCGALAIGFASLPLLAWIA
HHHHHHHHHHHCCCHHCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
QSPVILISGESLSTLDYGLLQIPVFGALILGNLTLARLTGKNSVQRLIKLGAGPMMLGLL
CCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCHHHHHHCCHHHHHHHHHHCCCHHHHHHH
IAALATLFSTHAYLWMTAGLSLYAYGIGLANAGLYRLTLFSSSVSKGTVSATMGMLSMMV
HHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHH
FTVGIELAKVAYVWGGSGLFNLFNLISGLCWLALVALFLSKRRSGDATPTPNAAL
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]