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The map label for this gene is phnI [H]

Identifier: 157155144

GI number: 157155144

Start: 4651485

End: 4652549

Strand: Reverse

Name: phnI [H]

Synonym: EcE24377A_4653

Alternate gene names: 157155144

Gene position: 4652549-4651485 (Counterclockwise)

Preceding gene: 157155684

Following gene: 157158826

Centisome position: 93.43

GC content: 61.22

Gene sequence:

>1065_bases
ATGTACGTTGCCGTGAAAGGGGGCGAGAAGGCGATCGACGCCGCTCACGCCCTGCAAGAGAGCCGACGCCGGGGCGATAC
CGATTTGCCTGAACTGAGCGTCGCCCAGATTGAACAGCAGCTTAATCTTGCGGTGGATCGCGTGATGACCGAAGGCGGCA
TTGCCGACCGCGAACTGGCGGCGCTGGCGCTGAAACAGGCCAGCGGCGATAACGTCGAAGCAATTTTCCTGCTGCGCGCC
TACCGCACCACGCTGGCGAAGCTCGCGGTAAGCGAGCCGCTCGACACCACAGAGATGCGTCTCGAACGGCGTATTTCGGC
GGTTTATAAAGACATTCCCGGCGGCCAGCTGCTTGGCCCAACCTACGACTACACCCATCGCCTGCTCGATTTCACCCTGC
TGGCAAACGGTGAAGCGCCGACGCTGACCACCGCCGACAGCGAACAACAGCCGTCGCCGCACGTTTTCAGCCTGCTGGCG
CGTCAGGGGCTGGTGAAGTTTGAAGAAGATAGCGGCGCACAGCCGGATGACATCACCCGCACGCCGCCGGTTTACCCCTG
CTCACGCTCCTCCCGCCTGCAACAATTAATGCGCGGGGACGAAGGCTATTTGCTGGCGCTGGCCTACTCCACCCAGCGTG
GTTACGGGCGCAATCACCCGTTCGCGGGCGAGATCCGCAGTGGTTACATCGACGTGTCGATTGTGCCGGAAGAGCTGGGA
TTTGCGGTAAACGTCGGCGAACTACTGATGACCGAGTGTGAAATGGTCAACGGTTTTATCGACCCGCCGGATGAGCCGCC
GCACTTCACGCGCGGCTACGGGCTGGTATTCGGCATGAGCGAGCGCAAAGCGATGGCAATGGCGCTGGTCGATCGTGCGT
TGCAGGCTCCGGAATACGGCGAGCACGCGACAGGCCCGGCGCAGGATGAAGAGTTTGTGCTGGCACATGCCGACAACGTC
GAAGCCGCAGGCTTTGTCTCGCACCTCAAACTCCCCCACTACGTCGATTTCCAGGCCGAACTGGAGCTACTCAAACGTCT
GCAACAGGAGAAGAACCATGGCTAA

Upstream 100 bases:

>100_bases
CCGAGCGCCCGCACCCGTTCCCGCTCGGCATCGACCTGATCCTGACCTGCGGCGAGCGCCTGCTGGCTATTCCGCGAACC
ACTCATGTGGAGGTGTGCTG

Downstream 100 bases:

>100_bases
TCTGAGCGGCTACAACTTTGCCTACCTCGACGAGCAGACCAAACGCATGATCCGCCGCGCTATCTTAAAAGCGGTGGCGA
TCCCCGGTTATCAGGTGCCG

Product: phosphonate metabolism protein PhnI

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 354; Mature: 354

Protein sequence:

>354_residues
MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLAVDRVMTEGGIADRELAALALKQASGDNVEAIFLLRA
YRTTLAKLAVSEPLDTTEMRLERRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
RQGLVKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRNHPFAGEIRSGYIDVSIVPEELG
FAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLVFGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNV
EAAGFVSHLKLPHYVDFQAELELLKRLQQEKNHG

Sequences:

>Translated_354_residues
MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLAVDRVMTEGGIADRELAALALKQASGDNVEAIFLLRA
YRTTLAKLAVSEPLDTTEMRLERRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
RQGLVKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRNHPFAGEIRSGYIDVSIVPEELG
FAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLVFGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNV
EAAGFVSHLKLPHYVDFQAELELLKRLQQEKNHG
>Mature_354_residues
MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLAVDRVMTEGGIADRELAALALKQASGDNVEAIFLLRA
YRTTLAKLAVSEPLDTTEMRLERRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
RQGLVKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRNHPFAGEIRSGYIDVSIVPEELG
FAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLVFGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNV
EAAGFVSHLKLPHYVDFQAELELLKRLQQEKNHG

Specific function: Belongs to an operon involved in alkylphosphonate uptake and C-P lyase. Exact function not known [H]

COG id: COG3626

COG function: function code P; Uncharacterized enzyme of phosphonate metabolism

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phnI family [H]

Homologues:

Organism=Escherichia coli, GI1790537, Length=354, Percent_Identity=98.8700564971751, Blast_Score=713, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008773 [H]

Pfam domain/function: PF05861 PhnI [H]

EC number: NA

Molecular weight: Translated: 39011; Mature: 39011

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLAVDRVMTEGGIADRELA
CEEEECCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
ALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTEMRLERRISAVYKDIPGGQLLGP
HHHHHHCCCCCCEEHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCC
TYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLARQGLVKFEEDSGAQPDDITR
CHHHHHHHHHEEEEECCCCCEEECCCCCCCCCHHHHHHHHHHCCEEECCCCCCCCCCCCC
TPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRNHPFAGEIRSGYIDVSIVPEELG
CCCCCCCCCHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCEEEEEEEHHHHC
FAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLVFGMSERKAMAMALVDRALQAPEYG
CEECHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHCCCCCC
EHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHYVDFQAELELLKRLQQEKNHG
CCCCCCCCCCCEEEEECCCCCHHHHHHHCCCCCEECHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLAVDRVMTEGGIADRELA
CEEEECCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
ALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTEMRLERRISAVYKDIPGGQLLGP
HHHHHHCCCCCCEEHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCC
TYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLARQGLVKFEEDSGAQPDDITR
CHHHHHHHHHEEEEECCCCCEEECCCCCCCCCHHHHHHHHHHCCEEECCCCCCCCCCCCC
TPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRNHPFAGEIRSGYIDVSIVPEELG
CCCCCCCCCHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCEEEEEEEHHHHC
FAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLVFGMSERKAMAMALVDRALQAPEYG
CEECHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHCCCCCC
EHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHYVDFQAELELLKRLQQEKNHG
CCCCCCCCCCCEEEEECCCCCHHHHHHHCCCCCEECHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2155230; 1840580; 7610040; 9278503 [H]