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The map label for this gene is phnJ [H]

Identifier: 157158826

GI number: 157158826

Start: 4650647

End: 4651492

Strand: Reverse

Name: phnJ [H]

Synonym: EcE24377A_4652

Alternate gene names: 157158826

Gene position: 4651492-4650647 (Counterclockwise)

Preceding gene: 157155144

Following gene: 157155315

Centisome position: 93.41

GC content: 57.68

Gene sequence:

>846_bases
ATGGCTAATCTGAGCGGCTACAACTTTGCCTACCTCGACGAGCAGACCAAACGCATGATCCGCCGCGCTATCTTAAAAGC
GGTGGCGATCCCCGGTTATCAGGTGCCGTTTGGCGGGCGCGAGATGCCGATGCCATACGGCTGGGGAACCGGCGGCATAC
AGCTCACCGCCAGCGTGATTGGCGAAAGCGACGTGCTGAAGGTGATTGACCAGGGTGCGGATGACACCACCAACGCCGTG
TCGATTCGCAACTTCTTTAAGCGCGTGACCGGGGTAAACACCACTGAACGTACGGACGATGCGACGCTTATCCAGACGCG
TCACCGCATCCCCGAAACGCCGCTGACCGAAGATCAGATCATTATCTTCCAGGTGCCAATCCCGGAACCGCTGCGCTTTA
TCGAGCCGCGCGAAACGGAAACCCGCACCATGCACGCGCTGGAAGAGTACGGCGTGATGCAGGTGAAACTGTATGAAGAT
ATCGCCCGCTTCGGTCATATCGCCACTACCTACGCCTATCCGGTGAAGGTGAACGGGCGCTACGTAATGGACCCGTCGCC
GATCCCGAAATTCGATAACCCAAAAATGGACATGATGCCCGCCCTGCAACTGTTCGGCGCGGGGCGCGAGAAGCGCATCT
ATGCGGTGCCGCCGTTTACCCGCGTGGAAAGTCTCGATTTCGACGATCACCCGTTCACCGTTCAGCAGTGGGATGAGCCA
TGCGCCATCTGCGGATCGACCCACAGCTATCTTGATGAAGTGGTGCTGGATGACGCTGGAAACCGCATGTTTGTCTGCTC
CGATACCGATTATTGCCGCCAACAGAGCGAGGCAAAAAACCAATGA

Upstream 100 bases:

>100_bases
GTCGAAGCCGCAGGCTTTGTCTCGCACCTCAAACTCCCCCACTACGTCGATTTCCAGGCCGAACTGGAGCTACTCAAACG
TCTGCAACAGGAGAAGAACC

Downstream 100 bases:

>100_bases
ATCAACCGTTACTTTCGGTCAATAACCTGACCCACCTTTACGCGCCGGGCAAAGGCTTTAGCGATGTCTCTTTTGATTTA
TGGCCGGGGGAAGTGCTGGG

Product: phosphonate metabolism protein PhnJ

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 281; Mature: 280

Protein sequence:

>281_residues
MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPMPYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAV
SIRNFFKRVTGVNTTERTDDATLIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPPFTRVESLDFDDHPFTVQQWDEP
CAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQSEAKNQ

Sequences:

>Translated_281_residues
MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPMPYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAV
SIRNFFKRVTGVNTTERTDDATLIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPPFTRVESLDFDDHPFTVQQWDEP
CAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQSEAKNQ
>Mature_280_residues
ANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPMPYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVS
IRNFFKRVTGVNTTERTDDATLIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYEDI
ARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPPFTRVESLDFDDHPFTVQQWDEPC
AICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQSEAKNQ

Specific function: Belongs to an operon involved in alkylphosphonate uptake and C-P lyase. Exact function not known [H]

COG id: COG3627

COG function: function code P; Uncharacterized enzyme of phosphonate metabolism

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phnJ family [H]

Homologues:

Organism=Escherichia coli, GI1790536, Length=281, Percent_Identity=99.644128113879, Blast_Score=586, Evalue=1e-169,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010306 [H]

Pfam domain/function: PF06007 PhnJ [H]

EC number: NA

Molecular weight: Translated: 31860; Mature: 31728

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPMPYGWGTGGIQLTASVI
CCCCCCCCEEEECHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCCCCEEEEEEHH
GESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDATLIQTRHRIPETPLTEDQI
CCHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCCCCCE
IIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYEDIARFGHIATTYAYPVKVNGR
EEEECCCCCCHHHCCCCCHHHHHHHHHHHCCCEEEEHHHHHHHHCCCHHEEEEEEEECCE
YVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPPFTRVESLDFDDHPFTVQQWDEP
EEECCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEECCCCHHHCCCCCCCCCCCHHCCCCC
CAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQSEAKNQ
HHHCCCHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHCCC
>Mature Secondary Structure 
ANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPMPYGWGTGGIQLTASVI
CCCCCCCEEEECHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCCCCEEEEEEHH
GESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDATLIQTRHRIPETPLTEDQI
CCHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCCCCCE
IIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYEDIARFGHIATTYAYPVKVNGR
EEEECCCCCCHHHCCCCCHHHHHHHHHHHCCCEEEEHHHHHHHHCCCHHEEEEEEEECCE
YVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPPFTRVESLDFDDHPFTVQQWDEP
EEECCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEECCCCHHHCCCCCCCCCCCHHCCCCC
CAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQSEAKNQ
HHHCCCHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2155230; 1840580; 7610040; 9278503 [H]