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The map label for this gene is ygiT

Identifier: 157162497

GI number: 157162497

Start: 3212682

End: 3213077

Strand: Reverse

Name: ygiT

Synonym: EcHS_A3199

Alternate gene names: 157162497

Gene position: 3213077-3212682 (Counterclockwise)

Preceding gene: 157162498

Following gene: 157162496

Centisome position: 69.19

GC content: 42.17

Gene sequence:

>396_bases
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Upstream 100 bases:

>100_bases
TGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTTTATCTTAAAATTACGGTAATTCATGACGTACTGATCGT
CTCGTTTAAGGAGAAGTAAT

Downstream 100 bases:

>100_bases
TTAATGGAAAAGCGGGGGCAACTCGCTTTTTTCTTTACCGGAGTGTGAGCCAAATCACTCGCGATAAGTTTGAGCGAACA
ATGCCACAGGCGTATTGTTG

Product: Cro/CI family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 131; Mature: 131

Protein sequence:

>131_residues
MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHGLYCVHCEESIMNKEESDAFMAQVKAFRASVNAETVAPEFIVKVRKK
LSLTQKEASEIFGGGVNAFSRYEKGNAQPHPSTIKLLRVLDKHPELLNEIR

Sequences:

>Translated_131_residues
MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHGLYCVHCEESIMNKEESDAFMAQVKAFRASVNAETVAPEFIVKVRKK
LSLTQKEASEIFGGGVNAFSRYEKGNAQPHPSTIKLLRVLDKHPELLNEIR
>Mature_131_residues
MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHGLYCVHCEESIMNKEESDAFMAQVKAFRASVNAETVAPEFIVKVRKK
LSLTQKEASEIFGGGVNAFSRYEKGNAQPHPSTIKLLRVLDKHPELLNEIR

Specific function: Unknown

COG id: COG1396

COG function: function code K; Predicted transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH cro/C1-type DNA-binding domain

Homologues:

Organism=Escherichia coli, GI1789398, Length=131, Percent_Identity=100, Blast_Score=273, Evalue=3e-75,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): YGIT_ECOLI (Q46864)

Other databases:

- EMBL:   U28377
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   C65089
- RefSeq:   AP_003571.1
- RefSeq:   NP_417493.1
- PDB:   3FMY
- PDB:   3GA8
- PDB:   3GN5
- PDB:   3HI2
- PDB:   3O9X
- PDBsum:   3FMY
- PDBsum:   3GA8
- PDBsum:   3GN5
- PDBsum:   3HI2
- PDBsum:   3O9X
- ProteinModelPortal:   Q46864
- SMR:   Q46864
- DIP:   DIP-12226N
- STRING:   Q46864
- EnsemblBacteria:   EBESCT00000003738
- EnsemblBacteria:   EBESCT00000017475
- GeneID:   945814
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW2989
- KEGG:   eco:b3021
- EchoBASE:   EB2840
- EcoGene:   EG13022
- eggNOG:   COG1396
- GeneTree:   EBGT00050000012049
- HOGENOM:   HBG491602
- OMA:   AAEIFGG
- ProtClustDB:   CLSK881376
- BioCyc:   EcoCyc:G7571-MONOMER
- BioCyc:   MetaCyc:G7571-MONOMER
- Genevestigator:   Q46864
- GO:   GO:0006350
- InterPro:   IPR001387
- InterPro:   IPR010982
- InterPro:   IPR022452
- InterPro:   IPR022453
- SMART:   SM00530
- TIGRFAMs:   TIGR03830
- TIGRFAMs:   TIGR03831

Pfam domain/function: SSF47413 Lambda_like_DNA

EC number: NA

Molecular weight: Translated: 14703; Mature: 14703

Theoretical pI: Translated: 9.27; Mature: 9.27

Prosite motif: PS50943 HTH_CROC1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHGLYCVHCEESIMNKEESDAFMAQVKAF
CCCCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHEEEECCHHHCCCCCHHHHHHHHHHH
RASVNAETVAPEFIVKVRKKLSLTQKEASEIFGGGVNAFSRYEKGNAQPHPSTIKLLRVL
HHHCCCHHCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHHHHHH
DKHPELLNEIR
HCCHHHHHHCC
>Mature Secondary Structure
MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHGLYCVHCEESIMNKEESDAFMAQVKAF
CCCCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHEEEECCHHHCCCCCHHHHHHHHHHH
RASVNAETVAPEFIVKVRKKLSLTQKEASEIFGGGVNAFSRYEKGNAQPHPSTIKLLRVL
HHHCCCHHCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHHHHHH
DKHPELLNEIR
HCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503