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The map label for this gene is ygiS

Identifier: 157162496

GI number: 157162496

Start: 3210942

End: 3212549

Strand: Reverse

Name: ygiS

Synonym: EcHS_A3198

Alternate gene names: 157162496

Gene position: 3212549-3210942 (Counterclockwise)

Preceding gene: 157162497

Following gene: 157162495

Centisome position: 69.18

GC content: 52.3

Gene sequence:

>1608_bases
ATGTATACGCGAAATTTATTATGGCTGGTCAGCCTGGTAAGTGCGGCTCCTCTCTACGCTGCTGACGTTCCCGCCAACAC
ACCGCTCGCCCCGCAACAAGTCTTTCGTTACAACAATCATAGCGACCCAGGTACGCTCGACCCGCAAAAGGTGGAGGAGA
ATACTGCCGCGCAGATTGTGCTGGATCTGTTTGAAGGTCTGGTATGGATGGACGGTGAAGGCCAGGTGCAGCCCGCTCAG
GCTGAACGCTGGGAGATACTGGACGGCGGCAAGCGCTATATTTTCCATCTGCGTAGCGGTTTGCAGTGGTCCGACGGTCA
GCCTCTGACGGCAGAGGATTTTGTCCTCGGCTGGCAGCGCGCGGTTGACCCGAAAACGGCAAGCCCTTTTGCTGGCTATC
TGGCACAGGCGCACATTAACAATGCCGCAGCTATTGTTGCGGGTAAAGCAGATGTTACATCGCTGGGTGTCAAAGCGACG
GATGATCGTACTCTTGAAGTTACGCTTGAGCAGCCGGTTCCTTGGTTCACGACGATGCTCGCCTGGCCGACGCTGTTCCC
GGTTCCTCATCATGTCATCGCTAAACATGGCGATAGCTGGAGTAAGCCAGAGAACATGGTTTACAACGGTGCCTTTGTGC
TTGATCAGTGGGTAGTTAACGAAAAGATTACTGCACGCAAAAATCCAAAGTACCGCGATGCGCAACATACAGTATTGCAA
CAGGTTGAGTATCTGGCGCTAGATAATTCGGTCACCGGCTATAACCGCTATCGCGCGGGAGAGGTCGATCTCACCTGGGT
TCCGGCGCAGCAAATTCCCGCCATTGAAAAATCACTGCCTGGCGAGCTACGAATTATTCCGCGTCTGAACAGCGAATATT
ACAACTTCAACCTTGAGAAACCGCCATTTAACGATGTGCGAGTGCGTCGGGCGCTATATCTTACGGTTGATCGACAGCTT
ATTGCGCAAAAGGTACTGGGGTTGAGAACGCCCGCAACCACGCTGACGCCGCCAGAGGTAAAAGGCTTTAGCGCGACGAC
GTTCGATGAACTGCAAAAGCCAATGAGTGAGCGCGTCGCGATGGCAAAAGCCTTGCTGAAACAGGCGGGATACGACGCCT
CTCATCCGCTACGCTTTGAGCTGTTCTACAACAAGTACGATCTGCATGAAAAGACCGCGATAGCGTTGTCTTCCGAATGG
AAAAAATGGCTGGGTGCACAGGTGACGCTGCGCACAATGGAGTGGAAAACCTATCTTGATGCCCGACGAGCCGGTGATTT
CATGCTGTCTCGGCAGTCGTGGGATGCGACGTACAATGATGCTTCCAGCTTCCTGAACACGCTCAAAAGCGATAGTGAAG
AAAACGTCGGTCACTGGAAAAATGCGCAGTATGACGCCTTACTAAACCAGGCCACGCAGATCACTGATGCGACAAAGCGT
AATGCGTTGTATCAGCAGGCAGAAGTGATCATCAACCAACAGGCACCGCTGATTCCTATCTACTATCAGCCGTTAATCAA
ACTGCTTAAACCCTACGTTGGCGGTTTTCCGCTGCATAATCCCCAGGATTATGTCTACAGCAAAGAGTTGTATATCAAGG
CACATTGA

Upstream 100 bases:

>100_bases
CTTTACCGGAGTGTGAGCCAAATCACTCGCGATAAGTTTGAGCGAACAATGCCACAGGCGTATTGTTGTTGGGCTTATAA
TTTCTCCGGTAAGGAATACC

Downstream 100 bases:

>100_bases
TGCCTTTATCGCCGTCGGAGCACGTCCGGCGGCGCTATTCACGCTGATCTCCTGTGACTCGACGCGGCAGATAATGTAGT
ATCTCCGGCAATATTGCCCC

Product: putative binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 535; Mature: 535

Protein sequence:

>535_residues
MYTRNLLWLVSLVSAAPLYAADVPANTPLAPQQVFRYNNHSDPGTLDPQKVEENTAAQIVLDLFEGLVWMDGEGQVQPAQ
AERWEILDGGKRYIFHLRSGLQWSDGQPLTAEDFVLGWQRAVDPKTASPFAGYLAQAHINNAAAIVAGKADVTSLGVKAT
DDRTLEVTLEQPVPWFTTMLAWPTLFPVPHHVIAKHGDSWSKPENMVYNGAFVLDQWVVNEKITARKNPKYRDAQHTVLQ
QVEYLALDNSVTGYNRYRAGEVDLTWVPAQQIPAIEKSLPGELRIIPRLNSEYYNFNLEKPPFNDVRVRRALYLTVDRQL
IAQKVLGLRTPATTLTPPEVKGFSATTFDELQKPMSERVAMAKALLKQAGYDASHPLRFELFYNKYDLHEKTAIALSSEW
KKWLGAQVTLRTMEWKTYLDARRAGDFMLSRQSWDATYNDASSFLNTLKSDSEENVGHWKNAQYDALLNQATQITDATKR
NALYQQAEVIINQQAPLIPIYYQPLIKLLKPYVGGFPLHNPQDYVYSKELYIKAH

Sequences:

>Translated_535_residues
MYTRNLLWLVSLVSAAPLYAADVPANTPLAPQQVFRYNNHSDPGTLDPQKVEENTAAQIVLDLFEGLVWMDGEGQVQPAQ
AERWEILDGGKRYIFHLRSGLQWSDGQPLTAEDFVLGWQRAVDPKTASPFAGYLAQAHINNAAAIVAGKADVTSLGVKAT
DDRTLEVTLEQPVPWFTTMLAWPTLFPVPHHVIAKHGDSWSKPENMVYNGAFVLDQWVVNEKITARKNPKYRDAQHTVLQ
QVEYLALDNSVTGYNRYRAGEVDLTWVPAQQIPAIEKSLPGELRIIPRLNSEYYNFNLEKPPFNDVRVRRALYLTVDRQL
IAQKVLGLRTPATTLTPPEVKGFSATTFDELQKPMSERVAMAKALLKQAGYDASHPLRFELFYNKYDLHEKTAIALSSEW
KKWLGAQVTLRTMEWKTYLDARRAGDFMLSRQSWDATYNDASSFLNTLKSDSEENVGHWKNAQYDALLNQATQITDATKR
NALYQQAEVIINQQAPLIPIYYQPLIKLLKPYVGGFPLHNPQDYVYSKELYIKAH
>Mature_535_residues
MYTRNLLWLVSLVSAAPLYAADVPANTPLAPQQVFRYNNHSDPGTLDPQKVEENTAAQIVLDLFEGLVWMDGEGQVQPAQ
AERWEILDGGKRYIFHLRSGLQWSDGQPLTAEDFVLGWQRAVDPKTASPFAGYLAQAHINNAAAIVAGKADVTSLGVKAT
DDRTLEVTLEQPVPWFTTMLAWPTLFPVPHHVIAKHGDSWSKPENMVYNGAFVLDQWVVNEKITARKNPKYRDAQHTVLQ
QVEYLALDNSVTGYNRYRAGEVDLTWVPAQQIPAIEKSLPGELRIIPRLNSEYYNFNLEKPPFNDVRVRRALYLTVDRQL
IAQKVLGLRTPATTLTPPEVKGFSATTFDELQKPMSERVAMAKALLKQAGYDASHPLRFELFYNKYDLHEKTAIALSSEW
KKWLGAQVTLRTMEWKTYLDARRAGDFMLSRQSWDATYNDASSFLNTLKSDSEENVGHWKNAQYDALLNQATQITDATKR
NALYQQAEVIINQQAPLIPIYYQPLIKLLKPYVGGFPLHNPQDYVYSKELYIKAH

Specific function: Probably part of a binding-protein-dependent transport system

COG id: COG4166

COG function: function code E; ABC-type oligopeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family

Homologues:

Organism=Escherichia coli, GI1789397, Length=535, Percent_Identity=100, Blast_Score=1102, Evalue=0.0,
Organism=Escherichia coli, GI1787495, Length=530, Percent_Identity=44.3396226415094, Blast_Score=451, Evalue=1e-128,
Organism=Escherichia coli, GI87081878, Length=529, Percent_Identity=42.34404536862, Blast_Score=404, Evalue=1e-114,
Organism=Escherichia coli, GI1789966, Length=512, Percent_Identity=26.3671875, Blast_Score=131, Evalue=1e-31,
Organism=Escherichia coli, GI1787052, Length=491, Percent_Identity=24.6435845213849, Blast_Score=118, Evalue=1e-27,
Organism=Escherichia coli, GI1787762, Length=529, Percent_Identity=24.1965973534972, Blast_Score=108, Evalue=9e-25,
Organism=Escherichia coli, GI1789887, Length=480, Percent_Identity=23.3333333333333, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1787551, Length=528, Percent_Identity=23.1060606060606, Blast_Score=84, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YGIS_ECOLI (Q46863)

Other databases:

- EMBL:   U28377
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   B65089
- RefSeq:   AP_003570.1
- RefSeq:   NP_417492.1
- ProteinModelPortal:   Q46863
- SMR:   Q46863
- STRING:   Q46863
- EnsemblBacteria:   EBESCT00000003373
- EnsemblBacteria:   EBESCT00000014923
- GeneID:   947140
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW2988
- KEGG:   eco:b3020
- EchoBASE:   EB2839
- EcoGene:   EG13021
- eggNOG:   COG4166
- GeneTree:   EBGT00070000031725
- HOGENOM:   HBG688042
- OMA:   KWVVNEK
- ProtClustDB:   PRK09755
- BioCyc:   EcoCyc:YGIS-MONOMER
- Genevestigator:   Q46863
- InterPro:   IPR000914

Pfam domain/function: PF00496 SBP_bac_5

EC number: NA

Molecular weight: Translated: 60694; Mature: 60694

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: PS01040 SBP_BACTERIAL_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYTRNLLWLVSLVSAAPLYAADVPANTPLAPQQVFRYNNHSDPGTLDPQKVEENTAAQIV
CCCCHHHHHHHHHHCCCCEEECCCCCCCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHH
LDLFEGLVWMDGEGQVQPAQAERWEILDGGKRYIFHLRSGLQWSDGQPLTAEDFVLGWQR
HHHHHCEEEECCCCCCCCCCCCCEEECCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHH
AVDPKTASPFAGYLAQAHINNAAAIVAGKADVTSLGVKATDDRTLEVTLEQPVPWFTTML
CCCCCCCCCHHHHHHHHHCCCCEEEEECCCCHHHCCEEECCCCEEEEEECCCCCHHHHHH
AWPTLFPVPHHVIAKHGDSWSKPENMVYNGAFVLDQWVVNEKITARKNPKYRDAQHTVLQ
HHHHHCCCCHHHHHCCCCCCCCCCCEEECCEEEEEHHHHCCCHHCCCCCCCCHHHHHHHH
QVEYLALDNSVTGYNRYRAGEVDLTWVPAQQIPAIEKSLPGELRIIPRLNSEYYNFNLEK
HHHHHHCCCCCCCCCCEECCCEEEEEECHHHCCHHHHCCCCCEEEEEECCCCEEEEECCC
PPFNDVRVRRALYLTVDRQLIAQKVLGLRTPATTLTPPEVKGFSATTFDELQKPMSERVA
CCCCHHHEEEEEEEEHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
MAKALLKQAGYDASHPLRFELFYNKYDLHEKTAIALSSEWKKWLGAQVTLRTMEWKTYLD
HHHHHHHHCCCCCCCCEEEEEEECCCCCCCHHEEEHHHHHHHHCCCEEEEEEEHHHHHHH
ARRAGDFMLSRQSWDATYNDASSFLNTLKSDSEENVGHWKNAQYDALLNQATQITDATKR
HHHCCCCEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
NALYQQAEVIINQQAPLIPIYYQPLIKLLKPYVGGFPLHNPQDYVYSKELYIKAH
HHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEEEC
>Mature Secondary Structure
MYTRNLLWLVSLVSAAPLYAADVPANTPLAPQQVFRYNNHSDPGTLDPQKVEENTAAQIV
CCCCHHHHHHHHHHCCCCEEECCCCCCCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHH
LDLFEGLVWMDGEGQVQPAQAERWEILDGGKRYIFHLRSGLQWSDGQPLTAEDFVLGWQR
HHHHHCEEEECCCCCCCCCCCCCEEECCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHH
AVDPKTASPFAGYLAQAHINNAAAIVAGKADVTSLGVKATDDRTLEVTLEQPVPWFTTML
CCCCCCCCCHHHHHHHHHCCCCEEEEECCCCHHHCCEEECCCCEEEEEECCCCCHHHHHH
AWPTLFPVPHHVIAKHGDSWSKPENMVYNGAFVLDQWVVNEKITARKNPKYRDAQHTVLQ
HHHHHCCCCHHHHHCCCCCCCCCCCEEECCEEEEEHHHHCCCHHCCCCCCCCHHHHHHHH
QVEYLALDNSVTGYNRYRAGEVDLTWVPAQQIPAIEKSLPGELRIIPRLNSEYYNFNLEK
HHHHHHCCCCCCCCCCEECCCEEEEEECHHHCCHHHHCCCCCEEEEEECCCCEEEEECCC
PPFNDVRVRRALYLTVDRQLIAQKVLGLRTPATTLTPPEVKGFSATTFDELQKPMSERVA
CCCCHHHEEEEEEEEHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
MAKALLKQAGYDASHPLRFELFYNKYDLHEKTAIALSSEWKKWLGAQVTLRTMEWKTYLD
HHHHHHHHCCCCCCCCEEEEEEECCCCCCCHHEEEHHHHHHHHCCCEEEEEEEHHHHHHH
ARRAGDFMLSRQSWDATYNDASSFLNTLKSDSEENVGHWKNAQYDALLNQATQITDATKR
HHHCCCCEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
NALYQQAEVIINQQAPLIPIYYQPLIKLLKPYVGGFPLHNPQDYVYSKELYIKAH
HHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 10493123