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The map label for this gene is manB [H]

Identifier: 157161526

GI number: 157161526

Start: 2152201

End: 2153583

Strand: Reverse

Name: manB [H]

Synonym: EcHS_A2172

Alternate gene names: 157161526

Gene position: 2153583-2152201 (Counterclockwise)

Preceding gene: 157161527

Following gene: 157161525

Centisome position: 46.38

GC content: 60.81

Gene sequence:

>1383_bases
ATGACACAGTTAACTTGTTTTAAGGCTTATGACATCCGTGGTGAACTGGGTGAGGAACTGAACGAGGACATCGCCTACCG
TATCGGGCGCGCCTACGGCGAATTTCTGAAACCCGGGAAGATAGTGGTGGGGGGGGATGTGCGCCTCACAAGCGAGTCGT
TGAAGCTGGCGCTGGCCCGCGGGTTAATGGACGCCGGTACCGATGTGCTGGACATCGGACTGAGCGGCACGGAAGAGATT
TACTTCGCCACCTTCCACCTCGGGGTGGACGGCGGCATCGAGGTGACGGCGAGCCACAACCCGATGAACTACAACGGCAT
GAAGCTGGTGCGCGAGAACGCGAAGCCCATCAGCGGCGACACCGGCCTGCGGGATATCCAGCGCCTGGCGGAGGAGAACC
AGTTCCCGCCGGTGGACCCGGCACGTCGCGGGACCCTGCGTCAGATTTCGGTGCTGAAGGAGTACGTCGACCACCTGATG
GGCTACGTGGACCTGGCGAACTTCACCCGTCCGCTGAAGCTGGTGGTGAACTCCGGCAACGGGGCGGCGGGGCACGTGAT
TGATGAGGTGGAGAAACGCTTCGCGGCGGCCGGGGTGCCGGTAACCTTTATCAAGGTGCATCACCAGCCGGATGGCCATT
TCCCTAACGGTATCCCTAATCCGCTGCTGCCGGAGTGCCGCCAGGATACCGCCGACGCGGTGCGCGAGCATCAGGCCGAC
ATGGGGATTGCCTTTGACGGCGACTTCGATCGCTGCTTCCTGTTCGATGACGAGGCGTCGTTTATCGAGGGGTATTACAT
TGTCGGCCTGCTGGCTGAGGCGTTCCTGCAGAAGCAGCCGGGAGCGAAAATCATTCACGACCCGCGCCTGACGTGGAATA
CGGTAGACATCGTGACCCGCAACGGCGGCCAGCCGGTGATGTCGAAGACGGGGCATGCGTTCATCAAGGAGCGGATGCGT
CAGGAAGACGCTATCTACGGCGGGGAGATGAGTGCGCACCATTACTTCCGCGATTTCGCCTACTGCGACAGCGGGATGAT
CCCGTGGCTGCTGGTGGCGGAGCTGCTGTGCCTGAAGAACAGCTCGCTGAAATCGCTGGTGGCGGACCGCCAGGCGGCGT
TCCCGGCGTCGGGGGAGATCAACCGCAAGCTGGGTAATGCGGCAGAGGCGATAGCGCGCATCCGGGCGCAGTATGAGCCG
GCGGCTGCACACATCGACACAACGGACGGGATCAGTATTGAATACCCTGAATGGCGCTTTAACCTGCGCACGTCTAACAC
CGAGCCGGTGGTGCGTCTGAACGTTGAGTCCAGAGCTGATGTGGCGCTTATGAATGAAAAAACGACCGAGCTGCTCAACC
TGTTAAAAGAGGAATTGCTTTGA

Upstream 100 bases:

>100_bases
TGCTGGAGATCCAGTCGGGGTCGTACCTCGGCGAGGACGACATTATTCGTATTAAAGACCAGTATGGTCGTTGCTAATTA
TTTTCGGGACAAGATGCAGA

Downstream 100 bases:

>100_bases
GTCCGATGTTTTCAGCGATCTATCGCTACCGTGGCTTTATTATTGACAGCGTTAAACGGGACTTTCAGTCCCGTTACCAA
ACCAGCTTCTTAGGCGCGGC

Product: phosphomannomutase

Products: NA

Alternate protein names: PMM [H]

Number of amino acids: Translated: 460; Mature: 459

Protein sequence:

>460_residues
MTQLTCFKAYDIRGELGEELNEDIAYRIGRAYGEFLKPGKIVVGGDVRLTSESLKLALARGLMDAGTDVLDIGLSGTEEI
YFATFHLGVDGGIEVTASHNPMNYNGMKLVRENAKPISGDTGLRDIQRLAEENQFPPVDPARRGTLRQISVLKEYVDHLM
GYVDLANFTRPLKLVVNSGNGAAGHVIDEVEKRFAAAGVPVTFIKVHHQPDGHFPNGIPNPLLPECRQDTADAVREHQAD
MGIAFDGDFDRCFLFDDEASFIEGYYIVGLLAEAFLQKQPGAKIIHDPRLTWNTVDIVTRNGGQPVMSKTGHAFIKERMR
QEDAIYGGEMSAHHYFRDFAYCDSGMIPWLLVAELLCLKNSSLKSLVADRQAAFPASGEINRKLGNAAEAIARIRAQYEP
AAAHIDTTDGISIEYPEWRFNLRTSNTEPVVRLNVESRADVALMNEKTTELLNLLKEELL

Sequences:

>Translated_460_residues
MTQLTCFKAYDIRGELGEELNEDIAYRIGRAYGEFLKPGKIVVGGDVRLTSESLKLALARGLMDAGTDVLDIGLSGTEEI
YFATFHLGVDGGIEVTASHNPMNYNGMKLVRENAKPISGDTGLRDIQRLAEENQFPPVDPARRGTLRQISVLKEYVDHLM
GYVDLANFTRPLKLVVNSGNGAAGHVIDEVEKRFAAAGVPVTFIKVHHQPDGHFPNGIPNPLLPECRQDTADAVREHQAD
MGIAFDGDFDRCFLFDDEASFIEGYYIVGLLAEAFLQKQPGAKIIHDPRLTWNTVDIVTRNGGQPVMSKTGHAFIKERMR
QEDAIYGGEMSAHHYFRDFAYCDSGMIPWLLVAELLCLKNSSLKSLVADRQAAFPASGEINRKLGNAAEAIARIRAQYEP
AAAHIDTTDGISIEYPEWRFNLRTSNTEPVVRLNVESRADVALMNEKTTELLNLLKEELL
>Mature_459_residues
TQLTCFKAYDIRGELGEELNEDIAYRIGRAYGEFLKPGKIVVGGDVRLTSESLKLALARGLMDAGTDVLDIGLSGTEEIY
FATFHLGVDGGIEVTASHNPMNYNGMKLVRENAKPISGDTGLRDIQRLAEENQFPPVDPARRGTLRQISVLKEYVDHLMG
YVDLANFTRPLKLVVNSGNGAAGHVIDEVEKRFAAAGVPVTFIKVHHQPDGHFPNGIPNPLLPECRQDTADAVREHQADM
GIAFDGDFDRCFLFDDEASFIEGYYIVGLLAEAFLQKQPGAKIIHDPRLTWNTVDIVTRNGGQPVMSKTGHAFIKERMRQ
EDAIYGGEMSAHHYFRDFAYCDSGMIPWLLVAELLCLKNSSLKSLVADRQAAFPASGEINRKLGNAAEAIARIRAQYEPA
AAHIDTTDGISIEYPEWRFNLRTSNTEPVVRLNVESRADVALMNEKTTELLNLLKEELL

Specific function: Involved in GDP-mannose biosynthesis which serves as the activated sugar nucleotide precursor for mannose residues in cell surface polysaccharides. This enzyme participates in synthesis of the LPS O9 antigen [H]

COG id: COG1109

COG function: function code G; Phosphomannomutase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphohexose mutase family [H]

Homologues:

Organism=Escherichia coli, GI1788361, Length=457, Percent_Identity=76.3676148796499, Blast_Score=734, Evalue=0.0,
Organism=Escherichia coli, GI1789566, Length=454, Percent_Identity=25.9911894273128, Blast_Score=92, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005844
- InterPro:   IPR016055
- InterPro:   IPR005845
- InterPro:   IPR005846
- InterPro:   IPR005843
- InterPro:   IPR016066
- InterPro:   IPR005841 [H]

Pfam domain/function: PF02878 PGM_PMM_I; PF02879 PGM_PMM_II; PF02880 PGM_PMM_III; PF00408 PGM_PMM_IV [H]

EC number: =5.4.2.8 [H]

Molecular weight: Translated: 50979; Mature: 50848

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: PS00710 PGM_PMM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQLTCFKAYDIRGELGEELNEDIAYRIGRAYGEFLKPGKIVVGGDVRLTSESLKLALAR
CCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCEEECHHHHHHHHHH
GLMDAGTDVLDIGLSGTEEIYFATFHLGVDGGIEVTASHNPMNYNGMKLVRENAKPISGD
HHHHCCCCEEEECCCCCCEEEEEEEEEECCCCEEEEECCCCCCCCCHHHHHCCCCCCCCC
TGLRDIQRLAEENQFPPVDPARRGTLRQISVLKEYVDHLMGYVDLANFTRPLKLVVNSGN
CCHHHHHHHHHCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCC
GAAGHVIDEVEKRFAAAGVPVTFIKVHHQPDGHFPNGIPNPLLPECRQDTADAVREHQAD
CCCCHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
MGIAFDGDFDRCFLFDDEASFIEGYYIVGLLAEAFLQKQPGAKIIHDPRLTWNTVDIVTR
CCEEECCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCEEEEEEEEEC
NGGQPVMSKTGHAFIKERMRQEDAIYGGEMSAHHYFRDFAYCDSGMIPWLLVAELLCLKN
CCCCCHHHHCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCC
SSLKSLVADRQAAFPASGEINRKLGNAAEAIARIRAQYEPAAAHIDTTDGISIEYPEWRF
CHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCEEE
NLRTSNTEPVVRLNVESRADVALMNEKTTELLNLLKEELL
EEECCCCCCEEEEECCCCCCEEEECHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TQLTCFKAYDIRGELGEELNEDIAYRIGRAYGEFLKPGKIVVGGDVRLTSESLKLALAR
CCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCEEECHHHHHHHHHH
GLMDAGTDVLDIGLSGTEEIYFATFHLGVDGGIEVTASHNPMNYNGMKLVRENAKPISGD
HHHHCCCCEEEECCCCCCEEEEEEEEEECCCCEEEEECCCCCCCCCHHHHHCCCCCCCCC
TGLRDIQRLAEENQFPPVDPARRGTLRQISVLKEYVDHLMGYVDLANFTRPLKLVVNSGN
CCHHHHHHHHHCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCC
GAAGHVIDEVEKRFAAAGVPVTFIKVHHQPDGHFPNGIPNPLLPECRQDTADAVREHQAD
CCCCHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
MGIAFDGDFDRCFLFDDEASFIEGYYIVGLLAEAFLQKQPGAKIIHDPRLTWNTVDIVTR
CCEEECCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCEEEEEEEEEC
NGGQPVMSKTGHAFIKERMRQEDAIYGGEMSAHHYFRDFAYCDSGMIPWLLVAELLCLKN
CCCCCHHHHCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCC
SSLKSLVADRQAAFPASGEINRKLGNAAEAIARIRAQYEPAAAHIDTTDGISIEYPEWRF
CHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCEEE
NLRTSNTEPVVRLNVESRADVALMNEKTTELLNLLKEELL
EEECCCCCCEEEEECCCCCCEEEECHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7515042; 9004408 [H]