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The map label for this gene is manC [H]

Identifier: 157161527

GI number: 157161527

Start: 2153607

End: 2155022

Strand: Reverse

Name: manC [H]

Synonym: EcHS_A2173

Alternate gene names: 157161527

Gene position: 2155022-2153607 (Counterclockwise)

Preceding gene: 157161528

Following gene: 157161526

Centisome position: 46.41

GC content: 59.25

Gene sequence:

>1416_bases
ATGTTACTTCCTGTAATTATGGCTGGCGGTACCGGCAGTCGTCTCTGGCCGATGTCTCGCGAGCTTTACCCGAAACAGTT
CCTCCGTCTGTTCGGGCAGAACTCCATGCTGCAGGAAACCATTATCCGACTCTCGGGCCTTGAAATCCATGAACCGATGG
TCATCTGTAACGAAGAGCATCGTTTCCTGGTGGCTGAACAGCTGCGCCAGCTCAATAAGCTGTCGAATAACATTATACTT
GAGCCGGTCGGGCGCAACACCGCGCCGGCCATCGCCCTGGCGGCCCTTCAGGCCACCCGCTACGGTGACGACCCGCTGAT
GCTGGTCCTCGCCGCCGACCATATCATCAACAATCAGTCGGCCTTCCACGACGCCATCCGCGTCGCCGAGCAGTATGCCG
ATGAAGGCCATCTGGTCACCTTCGGTATCGTGCCGAACGCCCCGGAAACCGGCTACGGCTACATCCAGCGCGGCGTGGCG
CTCACCGACAGCGCCCACGCCCCGTACCAGGTGGCCCGCTTCGTGGAGAAGCCGGACCGCGAGCGCGCCGAGGTCTACCT
CGCCTCCGGGGAGTACTACTGGAACAGCGGCATGTTTATGTTCCGTGCCAAAAAATACCTCTCTGAGCTGGCCAAATACC
GTCCGGATATCCTGGAAACCTGCCAGGCTGCGGTGAATGCCGCCGATAATGGCAGCGATTTCATCAATATCCCACATGAT
ATTTTCTGCGAGTGCCCGGACGAGTCCGTGGACTACGCCGTTATGGAGAAAACCGCTGATGCGGTGGTGGTCGGTCTCGA
TGCTGACTGGAGCGACGTTGGCTCCTGGTCCGCACTATGGGAGGTCAGCCCGAAAGACGGGCAGGGTAACGTTCTCAGCG
GTGACGCGTGGGTGCACAACAGCGAAAACTGCTACATCAACAGCGACGAGAAGCTGGTGGCGGCCATCGGTGTGGAGAAC
CTGGTGATTGTCAGCACCAAGGACGCCGTGCTGGTGATGAACCGCGAGCGCTCCCAGGACGTGAAGAAGGCGGTCGAGTT
CCTCAAGCAGAACCAGCGCAGCGAGTACAAGCGCCACCGTGAGATTTACCGTCCCTGGGGCCGCTGCGACGTGGTGGTCC
AGACCCCGCGCTTCAACGTCAACCGCATCACGGTGAAACCTGGCGGTGCCTTCTCGATGCAGATGCACCACCATCGCGCT
GAGCATTGGGTTATTCTCGCCGGCACCGGTCAGGTGACGGTCAACGGTAAGCAGTTCCTGTTGTCCGAGAACCAGTCCAC
CTTTATTCCGATTGGCGCCGAGCACTGCCTGGAAAACCCTGGCTGTATTCCGCTGGAAGTGCTGGAGATCCAGTCGGGGT
CGTACCTCGGCGAGGACGACATTATTCGTATTAAAGACCAGTATGGTCGTTGCTAA

Upstream 100 bases:

>100_bases
CGACAGCCAATGGCGGTAGCGTGCCTCAAAAAGGCCACGCCACCTGACGGTCGATTGATTGATAGTAAAATAAAGCAATT
ATTTATTTAAGGATCTCATT

Downstream 100 bases:

>100_bases
TTATTTTCGGGACAAGATGCAGAATGACACAGTTAACTTGTTTTAAGGCTTATGACATCCGTGGTGAACTGGGTGAGGAA
CTGAACGAGGACATCGCCTA

Product: mannose-1-phosphate guanylyltransferase

Products: NA

Alternate protein names: GDP-mannose pyrophosphorylase; GMP; GMPP [H]

Number of amino acids: Translated: 471; Mature: 471

Protein sequence:

>471_residues
MLLPVIMAGGTGSRLWPMSRELYPKQFLRLFGQNSMLQETIIRLSGLEIHEPMVICNEEHRFLVAEQLRQLNKLSNNIIL
EPVGRNTAPAIALAALQATRYGDDPLMLVLAADHIINNQSAFHDAIRVAEQYADEGHLVTFGIVPNAPETGYGYIQRGVA
LTDSAHAPYQVARFVEKPDRERAEVYLASGEYYWNSGMFMFRAKKYLSELAKYRPDILETCQAAVNAADNGSDFINIPHD
IFCECPDESVDYAVMEKTADAVVVGLDADWSDVGSWSALWEVSPKDGQGNVLSGDAWVHNSENCYINSDEKLVAAIGVEN
LVIVSTKDAVLVMNRERSQDVKKAVEFLKQNQRSEYKRHREIYRPWGRCDVVVQTPRFNVNRITVKPGGAFSMQMHHHRA
EHWVILAGTGQVTVNGKQFLLSENQSTFIPIGAEHCLENPGCIPLEVLEIQSGSYLGEDDIIRIKDQYGRC

Sequences:

>Translated_471_residues
MLLPVIMAGGTGSRLWPMSRELYPKQFLRLFGQNSMLQETIIRLSGLEIHEPMVICNEEHRFLVAEQLRQLNKLSNNIIL
EPVGRNTAPAIALAALQATRYGDDPLMLVLAADHIINNQSAFHDAIRVAEQYADEGHLVTFGIVPNAPETGYGYIQRGVA
LTDSAHAPYQVARFVEKPDRERAEVYLASGEYYWNSGMFMFRAKKYLSELAKYRPDILETCQAAVNAADNGSDFINIPHD
IFCECPDESVDYAVMEKTADAVVVGLDADWSDVGSWSALWEVSPKDGQGNVLSGDAWVHNSENCYINSDEKLVAAIGVEN
LVIVSTKDAVLVMNRERSQDVKKAVEFLKQNQRSEYKRHREIYRPWGRCDVVVQTPRFNVNRITVKPGGAFSMQMHHHRA
EHWVILAGTGQVTVNGKQFLLSENQSTFIPIGAEHCLENPGCIPLEVLEIQSGSYLGEDDIIRIKDQYGRC
>Mature_471_residues
MLLPVIMAGGTGSRLWPMSRELYPKQFLRLFGQNSMLQETIIRLSGLEIHEPMVICNEEHRFLVAEQLRQLNKLSNNIIL
EPVGRNTAPAIALAALQATRYGDDPLMLVLAADHIINNQSAFHDAIRVAEQYADEGHLVTFGIVPNAPETGYGYIQRGVA
LTDSAHAPYQVARFVEKPDRERAEVYLASGEYYWNSGMFMFRAKKYLSELAKYRPDILETCQAAVNAADNGSDFINIPHD
IFCECPDESVDYAVMEKTADAVVVGLDADWSDVGSWSALWEVSPKDGQGNVLSGDAWVHNSENCYINSDEKLVAAIGVEN
LVIVSTKDAVLVMNRERSQDVKKAVEFLKQNQRSEYKRHREIYRPWGRCDVVVQTPRFNVNRITVKPGGAFSMQMHHHRA
EHWVILAGTGQVTVNGKQFLLSENQSTFIPIGAEHCLENPGCIPLEVLEIQSGSYLGEDDIIRIKDQYGRC

Specific function: Involved in GDP-mannose biosynthesis which serves as the activated sugar nucleotide precursor for mannose residues in cell surface polysaccharides. This enzyme participates in synthesis of the LPS O9 antigen [H]

COG id: COG0836

COG function: function code M; Mannose-1-phosphate guanylyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mannose-6-phosphate isomerase type 2 family [H]

Homologues:

Organism=Escherichia coli, GI1788362, Length=472, Percent_Identity=61.4406779661017, Blast_Score=594, Evalue=1e-171,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011051
- InterPro:   IPR006375
- InterPro:   IPR001538
- InterPro:   IPR005835
- InterPro:   IPR014710 [H]

Pfam domain/function: PF01050 MannoseP_isomer; PF00483 NTP_transferase [H]

EC number: =2.7.7.13 [H]

Molecular weight: Translated: 52813; Mature: 52813

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLPVIMAGGTGSRLWPMSRELYPKQFLRLFGQNSMLQETIIRLSGLEIHEPMVICNEEH
CCEEEEEECCCCCCCCCCCHHCCHHHHHHHHCCCHHHHHHHHHHCCCEEECCEEEECCCC
RFLVAEQLRQLNKLSNNIILEPVGRNTAPAIALAALQATRYGDDPLMLVLAADHIINNQS
CCHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHCCCCCCEEEEEEEHHHHCCCH
AFHDAIRVAEQYADEGHLVTFGIVPNAPETGYGYIQRGVALTDSAHAPYQVARFVEKPDR
HHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHCCEEEECCCCCHHHHHHHHCCCCC
ERAEVYLASGEYYWNSGMFMFRAKKYLSELAKYRPDILETCQAAVNAADNGSDFINIPHD
CCEEEEEECCCEEECCCEEEEHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCEEECCHH
IFCECPDESVDYAVMEKTADAVVVGLDADWSDVGSWSALWEVSPKDGQGNVLSGDAWVHN
HEECCCCCCCCHHHHHCCCCEEEEEECCCCCCCCCCEEEEEECCCCCCCCEEECCCEEEC
SENCYINSDEKLVAAIGVENLVIVSTKDAVLVMNRERSQDVKKAVEFLKQNQRSEYKRHR
CCCEEECCCCCEEEEECCCEEEEEECCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHH
EIYRPWGRCDVVVQTPRFNVNRITVKPGGAFSMQMHHHRAEHWVILAGTGQVTVNGKQFL
HHHCCCCCEEEEEECCCCCCEEEEECCCCCEEEEHHHCCCCEEEEEEECCEEEECCCEEE
LSENQSTFIPIGAEHCLENPGCIPLEVLEIQSGSYLGEDDIIRIKDQYGRC
EECCCCEEEEECHHHHCCCCCCCEEEEEEECCCCCCCCCCEEEEECCCCCC
>Mature Secondary Structure
MLLPVIMAGGTGSRLWPMSRELYPKQFLRLFGQNSMLQETIIRLSGLEIHEPMVICNEEH
CCEEEEEECCCCCCCCCCCHHCCHHHHHHHHCCCHHHHHHHHHHCCCEEECCEEEECCCC
RFLVAEQLRQLNKLSNNIILEPVGRNTAPAIALAALQATRYGDDPLMLVLAADHIINNQS
CCHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHCCCCCCEEEEEEEHHHHCCCH
AFHDAIRVAEQYADEGHLVTFGIVPNAPETGYGYIQRGVALTDSAHAPYQVARFVEKPDR
HHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHCCEEEECCCCCHHHHHHHHCCCCC
ERAEVYLASGEYYWNSGMFMFRAKKYLSELAKYRPDILETCQAAVNAADNGSDFINIPHD
CCEEEEEECCCEEECCCEEEEHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCEEECCHH
IFCECPDESVDYAVMEKTADAVVVGLDADWSDVGSWSALWEVSPKDGQGNVLSGDAWVHN
HEECCCCCCCCHHHHHCCCCEEEEEECCCCCCCCCCEEEEEECCCCCCCCEEECCCEEEC
SENCYINSDEKLVAAIGVENLVIVSTKDAVLVMNRERSQDVKKAVEFLKQNQRSEYKRHR
CCCEEECCCCCEEEEECCCEEEEEECCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHH
EIYRPWGRCDVVVQTPRFNVNRITVKPGGAFSMQMHHHRAEHWVILAGTGQVTVNGKQFL
HHHCCCCCEEEEEECCCCCCEEEEECCCCCEEEEHHHCCCCEEEEEEECCEEEECCCEEE
LSENQSTFIPIGAEHCLENPGCIPLEVLEIQSGSYLGEDDIIRIKDQYGRC
EECCCCEEEEECHHHHCCCCCCCEEEEEEECCCCCCCCCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7515042; 9004408 [H]