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The map label for this gene is yafC [C]

Identifier: 156978116

GI number: 156978116

Start: 2018128

End: 2018997

Strand: Reverse

Name: yafC [C]

Synonym: VIBHAR_06921

Alternate gene names: 156978116

Gene position: 2018997-2018128 (Counterclockwise)

Preceding gene: 156978117

Following gene: 156978111

Centisome position: 91.61

GC content: 49.31

Gene sequence:

>870_bases
ATGGCGAATTGGGAAGGCGTGAGCGAATTTGTCGCGGTCGCAGAAACCAACAGCTTTACAGGCGCAGCAAACAAACTTAA
AACGTCGGTGGCTCAAATCAGCCGCCGCGTGTCAGCGTTGGAAGAGCGTTTAGCGGTTAAATTGCTGCATCGAACTACGC
GCAAAGTCTCTCTGACCGAAGCGGGGCAACTTTATTATCAGCAATGTAAAAACCTTGTCGAAGGTCTAGAGCTGGCAGAG
TTAGCGGTAACGCAGATGCAAGCCGAGCCGAAAGGTTTGCTCAAAGTGACTGCACCTGTGACTTACGGCGAAATGAACCT
CGCGCCGTTATTGCACCAGTTTATTGAGAAATACCCGCAGGTGAATCTAGATTTGAATCTGACTAACCAAAAGTTAGATT
TGATTGAGCAAGGCGTCGATGTGGCGATTCGTCTTGGGCGTTTGCAAGACTCTAGTTTGATTGCCAAGCGCTTGTCATCC
CGACAGCTTTACGTGTGTGCTAGCCCTGCGTACTTAGATCGCTCGGGTGAACCGCATACCTTGTCTGAGCTGAGCCATCA
CCAATGCTTGGTCGGTACCGCCGATTACTGGCACTTTAAAGAGCAAAAGCGTGAGAAGTCTTTGCGTGTTTCTGGCCGAA
TCAAATGCAACAGTGGTTTTGCTTTGGTCGATGCGGCAAAACGTGGCTTAGGCTTGGTACAGTTGCCGGACTACTACGTA
CAAGAAGCGTTAGACAATGGCGAGCTGGTGGAAGTGTTAACCGATTACCGAGACGATCGTGAAGGTATTTGGGCGCTGTA
CCCACAAAACCGAAACCTGTCGCCAAAAGTGCGTTTACTGATTGATTTCTTGGCTGAAAACATGAACTGA

Upstream 100 bases:

>100_bases
AAGTTGTACATTTAGTCGGCTATCCTTTTTAAACAGGTTTTCTCTGCATTTAACTTTGTGTGAGAATACGCATACATAGA
ATTCCACAGGAACCACATTC

Downstream 100 bases:

>100_bases
GAGACAGGCTAAATGAGAAAAATAAGCCAAGTTGCTATGAAAGCGACTTGGCTTTTATTTTTTGTCAGTGGGAATTACTG
CTCGTTTAACTCTGCATCTT

Product: transcriptional regulator

Products: NA

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 289; Mature: 288

Protein sequence:

>289_residues
MANWEGVSEFVAVAETNSFTGAANKLKTSVAQISRRVSALEERLAVKLLHRTTRKVSLTEAGQLYYQQCKNLVEGLELAE
LAVTQMQAEPKGLLKVTAPVTYGEMNLAPLLHQFIEKYPQVNLDLNLTNQKLDLIEQGVDVAIRLGRLQDSSLIAKRLSS
RQLYVCASPAYLDRSGEPHTLSELSHHQCLVGTADYWHFKEQKREKSLRVSGRIKCNSGFALVDAAKRGLGLVQLPDYYV
QEALDNGELVEVLTDYRDDREGIWALYPQNRNLSPKVRLLIDFLAENMN

Sequences:

>Translated_289_residues
MANWEGVSEFVAVAETNSFTGAANKLKTSVAQISRRVSALEERLAVKLLHRTTRKVSLTEAGQLYYQQCKNLVEGLELAE
LAVTQMQAEPKGLLKVTAPVTYGEMNLAPLLHQFIEKYPQVNLDLNLTNQKLDLIEQGVDVAIRLGRLQDSSLIAKRLSS
RQLYVCASPAYLDRSGEPHTLSELSHHQCLVGTADYWHFKEQKREKSLRVSGRIKCNSGFALVDAAKRGLGLVQLPDYYV
QEALDNGELVEVLTDYRDDREGIWALYPQNRNLSPKVRLLIDFLAENMN
>Mature_288_residues
ANWEGVSEFVAVAETNSFTGAANKLKTSVAQISRRVSALEERLAVKLLHRTTRKVSLTEAGQLYYQQCKNLVEGLELAEL
AVTQMQAEPKGLLKVTAPVTYGEMNLAPLLHQFIEKYPQVNLDLNLTNQKLDLIEQGVDVAIRLGRLQDSSLIAKRLSSR
QLYVCASPAYLDRSGEPHTLSELSHHQCLVGTADYWHFKEQKREKSLRVSGRIKCNSGFALVDAAKRGLGLVQLPDYYVQ
EALDNGELVEVLTDYRDDREGIWALYPQNRNLSPKVRLLIDFLAENMN

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=293, Percent_Identity=35.4948805460751, Blast_Score=170, Evalue=1e-43,
Organism=Escherichia coli, GI1789639, Length=290, Percent_Identity=33.7931034482759, Blast_Score=160, Evalue=8e-41,
Organism=Escherichia coli, GI1789440, Length=281, Percent_Identity=30.9608540925267, Blast_Score=159, Evalue=2e-40,
Organism=Escherichia coli, GI145693193, Length=286, Percent_Identity=32.5174825174825, Blast_Score=154, Evalue=5e-39,
Organism=Escherichia coli, GI1787128, Length=288, Percent_Identity=30.5555555555556, Blast_Score=145, Evalue=4e-36,
Organism=Escherichia coli, GI1787589, Length=282, Percent_Identity=30.8510638297872, Blast_Score=144, Evalue=6e-36,
Organism=Escherichia coli, GI87081978, Length=280, Percent_Identity=30.3571428571429, Blast_Score=143, Evalue=2e-35,
Organism=Escherichia coli, GI1788706, Length=255, Percent_Identity=28.6274509803922, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1790011, Length=258, Percent_Identity=26.3565891472868, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1786448, Length=282, Percent_Identity=24.822695035461, Blast_Score=78, Evalue=8e-16,
Organism=Escherichia coli, GI1789173, Length=250, Percent_Identity=29.6, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI157672245, Length=193, Percent_Identity=26.9430051813472, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1786713, Length=266, Percent_Identity=27.4436090225564, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI145693105, Length=131, Percent_Identity=31.2977099236641, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1787879, Length=285, Percent_Identity=24.5614035087719, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1787601, Length=119, Percent_Identity=28.5714285714286, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32487; Mature: 32355

Theoretical pI: Translated: 7.11; Mature: 7.11

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANWEGVSEFVAVAETNSFTGAANKLKTSVAQISRRVSALEERLAVKLLHRTTRKVSLTE
CCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHHH
AGQLYYQQCKNLVEGLELAELAVTQMQAEPKGLLKVTAPVTYGEMNLAPLLHQFIEKYPQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCEECCCHHHHHHHHHHHHCCC
VNLDLNLTNQKLDLIEQGVDVAIRLGRLQDSSLIAKRLSSRQLYVCASPAYLDRSGEPHT
EEEEEEECHHHHHHHHCCHHHEEEECCCCCHHHHHHHHCCCEEEEEECCHHHCCCCCCCH
LSELSHHQCLVGTADYWHFKEQKREKSLRVSGRIKCNSGFALVDAAKRGLGLVQLPDYYV
HHHHCCCEEEEECHHHHHHHHHHHHHCCCEEEEEEECCCEEEEHHHHHCCCEEECCHHHH
QEALDNGELVEVLTDYRDDREGIWALYPQNRNLSPKVRLLIDFLAENMN
HHHCCCCHHHHHHHHCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ANWEGVSEFVAVAETNSFTGAANKLKTSVAQISRRVSALEERLAVKLLHRTTRKVSLTE
CCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHHH
AGQLYYQQCKNLVEGLELAELAVTQMQAEPKGLLKVTAPVTYGEMNLAPLLHQFIEKYPQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCEECCCHHHHHHHHHHHHCCC
VNLDLNLTNQKLDLIEQGVDVAIRLGRLQDSSLIAKRLSSRQLYVCASPAYLDRSGEPHT
EEEEEEECHHHHHHHHCCHHHEEEECCCCCHHHHHHHHCCCEEEEEECCHHHCCCCCCCH
LSELSHHQCLVGTADYWHFKEQKREKSLRVSGRIKCNSGFALVDAAKRGLGLVQLPDYYV
HHHHCCCEEEEECHHHHHHHHHHHHHCCCEEEEEEECCCEEEEHHHHHCCCEEECCHHHH
QEALDNGELVEVLTDYRDDREGIWALYPQNRNLSPKVRLLIDFLAENMN
HHHCCCCHHHHHHHHCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8635745; 7542800 [H]