Click here to switch to the map view.

The map label for this gene is emrD [H]

Identifier: 156977943

GI number: 156977943

Start: 1871641

End: 1872846

Strand: Direct

Name: emrD [H]

Synonym: VIBHAR_06739

Alternate gene names: 156977943

Gene position: 1871641-1872846 (Clockwise)

Preceding gene: 156977942

Following gene: 156977944

Centisome position: 84.92

GC content: 50.17

Gene sequence:

>1206_bases
ATGTCCGCCTCGTTTCCATTAGCGAAACTTACCTTTTTAATCGCAATTCTGACTGCCGTAGGTCAAATGACTCAAACGAT
GTACGTGCCTTCTATTGGTCATATGGCGGGTGAGTTCTTGGTATCAGCGTCGTCACTTCAAGCTGTGATGGCGTGTTACC
TGATCCCTTACGGTCTTTCGCAGTTTGTGTACGGCACACTGTCTGATCGCCTTGGTCGCAAACCAATCATCGTTGCGGGT
TTGATCATTTATATTCTTGGTACTTTGGTGGCGCTATTTGCTCATGAGTACGAATGGTTCCTAGCCGGTAGCTTTATCCA
AGGTTTGGGTATCGGTTGTGGCGGTGCGATGTCTCGCACACTGACTCGTGACTGTTTTGAAGGCGCAGAACTGCACCGTG
CAAACAGCTTGATCAGCATGTGTGTAATTTTCTCGCCATTGATGGCCCCTGTACTAGGCGGTTACCTAACTGAAGCTTTC
GGTTGGCGCTCTAGCTACTTATTCCTTGCTCTGTTTGGTATCGCAGTAATGATCACCATGATGACAAGCATGATGGAAAC
TTTGCCGAAAGAGCGTCGCAAACATGAATCTGTGGCGAAGAGCTACAAGTTCGTGCTTTCAGACAAACGCTTCCAAGGCT
TCTTATTGGTGTTGGTTGCTACGTTCGCAGGCGTTGCAGTATTTGAAGCTGCAGCTGGTGTACTACTGGGCGGCGTGCTT
GGCTTGCCAGCAACCACAGTAAGCTTACTGTTTGTCTTGCCGATTCCGGGTTACTTAGTCGGCGCTGGTCTATCGAGCTA
CATCGCACAACGCCGTTCTGAGCGACGTGCTCTAAACGTTGGTCTAGTCGCTATCTTAGTTGGCTCAGCAGTGGTATTGA
TTCCGGGTCTGTTTGGTCTAACAACGGCGTTGACTCTGATTGGCGGTGCAACCATTTACTTCTTGGGTGCGGGTATTTTG
TTCCCAGCGGCAACAACAGGTGCACTTTCACCGTTCCCATACCACGCGGGCACTGGCGGTGCGATTTTAGGTGGCATGCA
AAACCTAGGTGCGGGTATCGCGACGCTGTTGGCTTCGTTCTTCCCGGCACAAGACCAAATGCCGCTAGGTTGTTTGATGA
TGGCAATGTCGATCATCGCCATGCTTGGTCTACGCTGGGTTAATCGCAAGCACGACCATTCAAACGAAATGCCACTGGCG
ATCTAA

Upstream 100 bases:

>100_bases
AATATCTCGCGCCTAGATTCCCTAATCACTTTGTTTATTTGGCTCTCCTACATGGAGCGCTAAGGCTTTTTACGTCTTAA
ATTTCTGGAGAGATACGCAA

Downstream 100 bases:

>100_bases
AATCTGACTCTAATCCCCACCGAGATAAAGAAAAGGGACATGCTGCAAAGTGTGTCCCTTTTCTGTTGCACACATCTTAC
CCTTTAGCTCTCATCCACCA

Product: multidrug resistance protein D

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 401; Mature: 400

Protein sequence:

>401_residues
MSASFPLAKLTFLIAILTAVGQMTQTMYVPSIGHMAGEFLVSASSLQAVMACYLIPYGLSQFVYGTLSDRLGRKPIIVAG
LIIYILGTLVALFAHEYEWFLAGSFIQGLGIGCGGAMSRTLTRDCFEGAELHRANSLISMCVIFSPLMAPVLGGYLTEAF
GWRSSYLFLALFGIAVMITMMTSMMETLPKERRKHESVAKSYKFVLSDKRFQGFLLVLVATFAGVAVFEAAAGVLLGGVL
GLPATTVSLLFVLPIPGYLVGAGLSSYIAQRRSERRALNVGLVAILVGSAVVLIPGLFGLTTALTLIGGATIYFLGAGIL
FPAATTGALSPFPYHAGTGGAILGGMQNLGAGIATLLASFFPAQDQMPLGCLMMAMSIIAMLGLRWVNRKHDHSNEMPLA
I

Sequences:

>Translated_401_residues
MSASFPLAKLTFLIAILTAVGQMTQTMYVPSIGHMAGEFLVSASSLQAVMACYLIPYGLSQFVYGTLSDRLGRKPIIVAG
LIIYILGTLVALFAHEYEWFLAGSFIQGLGIGCGGAMSRTLTRDCFEGAELHRANSLISMCVIFSPLMAPVLGGYLTEAF
GWRSSYLFLALFGIAVMITMMTSMMETLPKERRKHESVAKSYKFVLSDKRFQGFLLVLVATFAGVAVFEAAAGVLLGGVL
GLPATTVSLLFVLPIPGYLVGAGLSSYIAQRRSERRALNVGLVAILVGSAVVLIPGLFGLTTALTLIGGATIYFLGAGIL
FPAATTGALSPFPYHAGTGGAILGGMQNLGAGIATLLASFFPAQDQMPLGCLMMAMSIIAMLGLRWVNRKHDHSNEMPLA
I
>Mature_400_residues
SASFPLAKLTFLIAILTAVGQMTQTMYVPSIGHMAGEFLVSASSLQAVMACYLIPYGLSQFVYGTLSDRLGRKPIIVAGL
IIYILGTLVALFAHEYEWFLAGSFIQGLGIGCGGAMSRTLTRDCFEGAELHRANSLISMCVIFSPLMAPVLGGYLTEAFG
WRSSYLFLALFGIAVMITMMTSMMETLPKERRKHESVAKSYKFVLSDKRFQGFLLVLVATFAGVAVFEAAAGVLLGGVLG
LPATTVSLLFVLPIPGYLVGAGLSSYIAQRRSERRALNVGLVAILVGSAVVLIPGLFGLTTALTLIGGATIYFLGAGILF
PAATTGALSPFPYHAGTGGAILGGMQNLGAGIATLLASFFPAQDQMPLGCLMMAMSIIAMLGLRWVNRKHDHSNEMPLAI

Specific function: Multidrug resistance pump that participates in a low energy shock adaptative response [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily [H]

Homologues:

Organism=Escherichia coli, GI87082312, Length=346, Percent_Identity=42.7745664739884, Blast_Score=264, Evalue=6e-72,
Organism=Escherichia coli, GI48994889, Length=342, Percent_Identity=23.6842105263158, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1788509, Length=356, Percent_Identity=25, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1790146, Length=363, Percent_Identity=25.068870523416, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1787065, Length=303, Percent_Identity=25.7425742574257, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1790794, Length=325, Percent_Identity=22.7692307692308, Blast_Score=69, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004734 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 42463; Mature: 42332

Theoretical pI: Translated: 9.08; Mature: 9.08

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
SASFPLAKLTFLIAILTAVGQMTQTMYVPSIGHMAGEFLVSASSLQAVMACYLIPYGLS
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QFVYGTLSDRLGRKPIIVAGLIIYILGTLVALFAHEYEWFLAGSFIQGLGIGCGGAMSRT
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
LTRDCFEGAELHRANSLISMCVIFSPLMAPVLGGYLTEAFGWRSSYLFLALFGIAVMITM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
MTSMMETLPKERRKHESVAKSYKFVLSDKRFQGFLLVLVATFAGVAVFEAAAGVLLGGVL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLPATTVSLLFVLPIPGYLVGAGLSSYIAQRRSERRALNVGLVAILVGSAVVLIPGLFGL
CCHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
TTALTLIGGATIYFLGAGILFPAATTGALSPFPYHAGTGGAILGGMQNLGAGIATLLASF
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHCHHHHHHHHHHHHHHH
FPAQDQMPLGCLMMAMSIIAMLGLRWVNRKHDHSNEMPLAI
CCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8240355; 7686882; 9278503 [H]