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The map label for this gene is glgX [H]

Identifier: 156976050

GI number: 156976050

Start: 82177

End: 84225

Strand: Reverse

Name: glgX [H]

Synonym: VIBHAR_04820

Alternate gene names: 156976050

Gene position: 84225-82177 (Counterclockwise)

Preceding gene: 156976055

Following gene: 156976049

Centisome position: 3.82

GC content: 47.24

Gene sequence:

>2049_bases
GTGGTTGACCTTATTAGGACAAAGTTTTTTACTTATAGTTTCTTAAAGGTAGCTATAAAATTAAAAAAAGATTCGCTGAT
GACACAACTTCACTCTCGCCCATACCCACTCGGCGCAACTGTGAGTACAGAAGGTTGTAACTTCTCTATTCATGCTCCGA
GTAGTAAAGACATTCAACTTGCGCTATTTGCGGAAGACGGTACCTATACACTGCACGACTTGTCAGGCGAATACGCAGGC
ATTCGTCATACTTTCATCCCAGACATTAAACCTGGACAGAAGTATGGCTATATAGTGACACATCAAGGCCAACCTTTGCT
GATCTCCGATCCTTACGCAAAGTCGATTGATAAAGCCCTTCACTACCAACCTCCCTTCTCCCCTATAAAAAGCTTTGATA
TGCCCAAATGTGTTGTGCTTGAAGACAACTTCGATTGGCAAGACACCGATCATCCGCGCATACCGCGTGAAGAGATGGTG
CTATTCGAAACCCATGTGAAAGGGTTAACTCAACAGCACCCAGAGGTCGCCAGTGATACCAAAGGCCGTTATTTAGGGTT
GTCTTCGCCAGAAATGCTGGCGTTCTTCAAACAGCAAAACATCAATACGCTGCAATTGCTACCCGTGGCAGCGTGTATGC
ATGAGACTCACTTGCTCAAGATGGATAAAGTGAACTATTGGGGTTACAACCCATACTTGTTCATGGTGCCAGATCCTCGC
TATGCAGAAAAAGACGCGGTGACCGAACTCAAAACCACCATCCGCGAGCTGCATAAACACGGCATCGAAGTGATTCTTGA
TGTGGTGTATAACCACACGGCGGAAGGCGGTGAAGGGTCTACTATCTTCAACTTAAAAGCACTCGATTCACGATTCTATA
TCAAACATGGTCAGCACTTCGCGAACTTTACTGGTTGTGGCAACACAGTCGACTTAACTTACCAGCCAGCAATGAATCTC
GTTATGGATACACTGCGATACTGGGTGAGCGAATTTAAAGTTGATGGTTTCCGTTTTGACTTGGCAGCAACGCTAGGTCG
CCAAGGCGACAATTACAACCCTGAAGCGGCCTTCTTTAAAGCGGTTGCACAAGACCCCACTCTTCGCGAAACCAAGCTGA
TTGCAGAGCCTTGGGACATCGGTCCTAACGGTTACCAAGTGGGTAACTTCCCATTTGGTTGGAACGAATGTAACGATAAA
CTTCGCGACATCTCCCGCAGCTTCTGGCGCGGTGACCAAGGCTACCTAAAAGAATTTGCGACTCGTTTGATGGGCTCTCG
TGATATCTACAGCGCAGCAAACTGGCCTCACAAACTCACGGTCAACTACATCACATATCACGATGGTTTCACCTTGCAAG
ATCTCGTGTCTTACAAACACAAGCACAACGAAGCGAACGGTGAGGAAAACCGCGACGGCCACGGCGACAACCGCTCTGAA
AACTACGGCGTGGAAGGCGAAACCGAAAATCTGATGATCATCGCCACTCGTGAAAAACAAAAGCGCAACTTAATGGCGAG
CTTGCTGTTTGCATTTGGTATCCCTCATATTCTGACCGCGGATGTTTTGTCGCACACGCAAGGCGGTAACAACAATGCTT
ACTGTCAGGATAACGAAACCAGCTGGTTGAACTGGACGAAGTCTGAGCGCAAGGAAGATTTCAAAGCATGGATTTCACAA
ATGGTGGCAAACCGTAATCAGTATATGGTGCCATTCATTCGCGCTTTTAGTGGTGAAAATCGTAATAACAACCGCATCTT
CTGGCGTCGCATTAATGGTAGCTTGATGGAACACGACGATTGGAACCGTTTAAGCTCGGTAGCGCTGCATCTAGGGATTG
GTAAAGAGGGGCAAGAGTTGGTGTACTTGATCAACCAAACCAACGCGCCAGCGTGTTTCAAGCTGCCAAGTGAGCGTGGT
CAAGAGTGGGTGACCGTGTGTGATACCAATGTACGCAACCTAAACCCAGGTCATGCAGAGGGCGAGGTACTGCTTTCTCC
GACTTCGATGGCTATTTTACATTATCAGCCAACCAAAAAGCTTAGCTGA

Upstream 100 bases:

>100_bases
CTTCAGTTTATGGCCGCTACAAAGTGATAGCCATTTCATATTTTCGTAAAAAATGGGTCGTAGAAAGTAATTTAATTACA
GTGATAATTTTCGATGTAAG

Downstream 100 bases:

>100_bases
CCCAAACCGCAGCTTATTCCTACAAACTCTGACAAACCCAGCAACACGCTGGGTTTGGTTTATCGACATTTCGAGTAGAG
GTCCATCAACTAGGCCAAAT

Product: glycogen operon protein GlgX

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 682; Mature: 682

Protein sequence:

>682_residues
MVDLIRTKFFTYSFLKVAIKLKKDSLMTQLHSRPYPLGATVSTEGCNFSIHAPSSKDIQLALFAEDGTYTLHDLSGEYAG
IRHTFIPDIKPGQKYGYIVTHQGQPLLISDPYAKSIDKALHYQPPFSPIKSFDMPKCVVLEDNFDWQDTDHPRIPREEMV
LFETHVKGLTQQHPEVASDTKGRYLGLSSPEMLAFFKQQNINTLQLLPVAACMHETHLLKMDKVNYWGYNPYLFMVPDPR
YAEKDAVTELKTTIRELHKHGIEVILDVVYNHTAEGGEGSTIFNLKALDSRFYIKHGQHFANFTGCGNTVDLTYQPAMNL
VMDTLRYWVSEFKVDGFRFDLAATLGRQGDNYNPEAAFFKAVAQDPTLRETKLIAEPWDIGPNGYQVGNFPFGWNECNDK
LRDISRSFWRGDQGYLKEFATRLMGSRDIYSAANWPHKLTVNYITYHDGFTLQDLVSYKHKHNEANGEENRDGHGDNRSE
NYGVEGETENLMIIATREKQKRNLMASLLFAFGIPHILTADVLSHTQGGNNNAYCQDNETSWLNWTKSERKEDFKAWISQ
MVANRNQYMVPFIRAFSGENRNNNRIFWRRINGSLMEHDDWNRLSSVALHLGIGKEGQELVYLINQTNAPACFKLPSERG
QEWVTVCDTNVRNLNPGHAEGEVLLSPTSMAILHYQPTKKLS

Sequences:

>Translated_682_residues
MVDLIRTKFFTYSFLKVAIKLKKDSLMTQLHSRPYPLGATVSTEGCNFSIHAPSSKDIQLALFAEDGTYTLHDLSGEYAG
IRHTFIPDIKPGQKYGYIVTHQGQPLLISDPYAKSIDKALHYQPPFSPIKSFDMPKCVVLEDNFDWQDTDHPRIPREEMV
LFETHVKGLTQQHPEVASDTKGRYLGLSSPEMLAFFKQQNINTLQLLPVAACMHETHLLKMDKVNYWGYNPYLFMVPDPR
YAEKDAVTELKTTIRELHKHGIEVILDVVYNHTAEGGEGSTIFNLKALDSRFYIKHGQHFANFTGCGNTVDLTYQPAMNL
VMDTLRYWVSEFKVDGFRFDLAATLGRQGDNYNPEAAFFKAVAQDPTLRETKLIAEPWDIGPNGYQVGNFPFGWNECNDK
LRDISRSFWRGDQGYLKEFATRLMGSRDIYSAANWPHKLTVNYITYHDGFTLQDLVSYKHKHNEANGEENRDGHGDNRSE
NYGVEGETENLMIIATREKQKRNLMASLLFAFGIPHILTADVLSHTQGGNNNAYCQDNETSWLNWTKSERKEDFKAWISQ
MVANRNQYMVPFIRAFSGENRNNNRIFWRRINGSLMEHDDWNRLSSVALHLGIGKEGQELVYLINQTNAPACFKLPSERG
QEWVTVCDTNVRNLNPGHAEGEVLLSPTSMAILHYQPTKKLS
>Mature_682_residues
MVDLIRTKFFTYSFLKVAIKLKKDSLMTQLHSRPYPLGATVSTEGCNFSIHAPSSKDIQLALFAEDGTYTLHDLSGEYAG
IRHTFIPDIKPGQKYGYIVTHQGQPLLISDPYAKSIDKALHYQPPFSPIKSFDMPKCVVLEDNFDWQDTDHPRIPREEMV
LFETHVKGLTQQHPEVASDTKGRYLGLSSPEMLAFFKQQNINTLQLLPVAACMHETHLLKMDKVNYWGYNPYLFMVPDPR
YAEKDAVTELKTTIRELHKHGIEVILDVVYNHTAEGGEGSTIFNLKALDSRFYIKHGQHFANFTGCGNTVDLTYQPAMNL
VMDTLRYWVSEFKVDGFRFDLAATLGRQGDNYNPEAAFFKAVAQDPTLRETKLIAEPWDIGPNGYQVGNFPFGWNECNDK
LRDISRSFWRGDQGYLKEFATRLMGSRDIYSAANWPHKLTVNYITYHDGFTLQDLVSYKHKHNEANGEENRDGHGDNRSE
NYGVEGETENLMIIATREKQKRNLMASLLFAFGIPHILTADVLSHTQGGNNNAYCQDNETSWLNWTKSERKEDFKAWISQ
MVANRNQYMVPFIRAFSGENRNNNRIFWRRINGSLMEHDDWNRLSSVALHLGIGKEGQELVYLINQTNAPACFKLPSERG
QEWVTVCDTNVRNLNPGHAEGEVLLSPTSMAILHYQPTKKLS

Specific function: Hydrolyzes The Alpha-1,6-Glucosidic Linkages In Glycogen Which Has First Been Partially Depolymerized By Phosphorylase. Shows Only Very Little Activity With Native Glycogen. [C]

COG id: COG1523

COG function: function code G; Type II secretory pathway, pullulanase PulA and related glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI2367229, Length=545, Percent_Identity=41.651376146789, Blast_Score=428, Evalue=1e-121,
Organism=Drosophila melanogaster, GI28573410, Length=251, Percent_Identity=25.0996015936255, Blast_Score=67, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR011837
- InterPro:   IPR013783
- InterPro:   IPR014756 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02922 CBM_48 [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 77953; Mature: 77953

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVDLIRTKFFTYSFLKVAIKLKKDSLMTQLHSRPYPLGATVSTEGCNFSIHAPSSKDIQL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCEEEEECCCCCCEEE
ALFAEDGTYTLHDLSGEYAGIRHTFIPDIKPGQKYGYIVTHQGQPLLISDPYAKSIDKAL
EEEECCCCEEEEECCCCCCCEEEEECCCCCCCCCCCEEEEECCCEEEEECCHHHHHHHHH
HYQPPFSPIKSFDMPKCVVLEDNFDWQDTDHPRIPREEMVLFETHVKGLTQQHPEVASDT
CCCCCCCHHHCCCCCEEEEEECCCCCCCCCCCCCCHHHEEEHHHHHHHHHHCCCCCCCCC
KGRYLGLSSPEMLAFFKQQNINTLQLLPVAACMHETHLLKMDKVNYWGYNPYLFMVPDPR
CCCEECCCCHHHHHHHHHCCCCEEEHHHHHHHHHHHHEEEEECEEECCCCCEEEECCCCC
YAEKDAVTELKTTIRELHKHGIEVILDVVYNHTAEGGEGSTIFNLKALDSRFYIKHGQHF
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCEEEEECCCCE
ANFTGCGNTVDLTYQPAMNLVMDTLRYWVSEFKVDGFRFDLAATLGRQGDNYNPEAAFFK
ECCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHCCCCCCCCCHHHHHH
AVAQDPTLRETKLIAEPWDIGPNGYQVGNFPFGWNECNDKLRDISRSFWRGDQGYLKEFA
HHHCCCCCCHHEEECCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHH
TRLMGSRDIYSAANWPHKLTVNYITYHDGFTLQDLVSYKHKHNEANGEENRDGHGDNRSE
HHHCCCCCHHHHCCCCCEEEEEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
NYGVEGETENLMIIATREKQKRNLMASLLFAFGIPHILTADVLSHTQGGNNNAYCQDNET
CCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCEEECCCCC
SWLNWTKSERKEDFKAWISQMVANRNQYMVPFIRAFSGENRNNNRIFWRRINGSLMEHDD
CCCCCCHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHCCCCCCCCEEEEEECCCCEECCCC
WNRLSSVALHLGIGKEGQELVYLINQTNAPACFKLPSERGQEWVTVCDTNVRNLNPGHAE
HHHHHHEEEEECCCCCCCEEEEEEECCCCCEEEECCHHCCCCEEEEECCCCCCCCCCCCC
GEVLLSPTSMAILHYQPTKKLS
CEEEECCCEEEEEEECCCCCCC
>Mature Secondary Structure
MVDLIRTKFFTYSFLKVAIKLKKDSLMTQLHSRPYPLGATVSTEGCNFSIHAPSSKDIQL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCEEEEECCCCCCEEE
ALFAEDGTYTLHDLSGEYAGIRHTFIPDIKPGQKYGYIVTHQGQPLLISDPYAKSIDKAL
EEEECCCCEEEEECCCCCCCEEEEECCCCCCCCCCCEEEEECCCEEEEECCHHHHHHHHH
HYQPPFSPIKSFDMPKCVVLEDNFDWQDTDHPRIPREEMVLFETHVKGLTQQHPEVASDT
CCCCCCCHHHCCCCCEEEEEECCCCCCCCCCCCCCHHHEEEHHHHHHHHHHCCCCCCCCC
KGRYLGLSSPEMLAFFKQQNINTLQLLPVAACMHETHLLKMDKVNYWGYNPYLFMVPDPR
CCCEECCCCHHHHHHHHHCCCCEEEHHHHHHHHHHHHEEEEECEEECCCCCEEEECCCCC
YAEKDAVTELKTTIRELHKHGIEVILDVVYNHTAEGGEGSTIFNLKALDSRFYIKHGQHF
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCEEEEECCCCE
ANFTGCGNTVDLTYQPAMNLVMDTLRYWVSEFKVDGFRFDLAATLGRQGDNYNPEAAFFK
ECCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHCCCCCCCCCHHHHHH
AVAQDPTLRETKLIAEPWDIGPNGYQVGNFPFGWNECNDKLRDISRSFWRGDQGYLKEFA
HHHCCCCCCHHEEECCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHH
TRLMGSRDIYSAANWPHKLTVNYITYHDGFTLQDLVSYKHKHNEANGEENRDGHGDNRSE
HHHCCCCCHHHHCCCCCEEEEEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
NYGVEGETENLMIIATREKQKRNLMASLLFAFGIPHILTADVLSHTQGGNNNAYCQDNET
CCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCEEECCCCC
SWLNWTKSERKEDFKAWISQMVANRNQYMVPFIRAFSGENRNNNRIFWRRINGSLMEHDD
CCCCCCHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHCCCCCCCCEEEEEECCCCEECCCC
WNRLSSVALHLGIGKEGQELVYLINQTNAPACFKLPSERGQEWVTVCDTNVRNLNPGHAE
HHHHHHEEEEECCCCCCCEEEEEEECCCCCEEEECCHHCCCCEEEEECCCCCCCCCCCCC
GEVLLSPTSMAILHYQPTKKLS
CEEEECCCEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Glycosylases; Glycosidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]