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The map label for this gene is purR [H]

Identifier: 156976042

GI number: 156976042

Start: 73409

End: 74416

Strand: Reverse

Name: purR [H]

Synonym: VIBHAR_04812

Alternate gene names: 156976042

Gene position: 74416-73409 (Counterclockwise)

Preceding gene: 156976043

Following gene: 156976041

Centisome position: 3.38

GC content: 48.02

Gene sequence:

>1008_bases
ATGAATCCTGCAAAACAAACCATAACATCGAAGGACGTCGCCAAACTGGCTGGCGTTTCTCAATCTACTGTATCGCGTGT
GTTTGTTCCGGGCAGCTCAGTGTCAGAAAAGACAAAGCAAAAGGTCTTCGAAGCCGCGAAAGCCCTAAACTACCGTCCAA
ATGCTTTTGCACGCAGCTTGACCACCAACGAATCCAAGTTGATCGGTTTGGTATTCCCCGATGCGGACTACCCTATCCAC
ATGAAAACACTTCAATTGATTTCAAGTGAATTGCAGAAGCAAGGCTACTCAGCGGTGTTGATTCCATGGCAAGTGGATGA
TGAAGACAACCACTCAATTCCGAACATCTTCCAATACCGCGTAGATGGTGTCATTGCCGCTTCCGCTACCTTCAACAAGT
CACTGTATGAAGAGTGTGAAGAGTTCAATATCCCTATCGTTCAGTACGCGCGAGTAGTCGAAGGCACTAAGAGCAGTTAC
GTTATCAGCGACAACTACGAAGCTGGTCAACAAGCGGCGCAACTGCTGCACGAACGTGGAGTGAAAAACGCGGTTTACCT
TACGGGTGAAGTACCAACCTTTACCAATGACGAGCGCCAAAGCGGATTCAGCTCAGAGTTTGAAGACTTAACGGGCAAAA
CGCCGCGCATCGTTGAAGCGAGCTACGATTACGCAAGTTCACTGGATAAAGTGCGTGCTATGCTTGCAGACAAGAATCGC
CCAGAAGCCGTATTCTGCGCGACTGACAACCTTGCAATGGCGGTAATGGACATTGCGCGAATCGAGTGTGGTATTCGTAT
CCCTCAAGATCTGAAAGTCATCGGCTTCGACAACATTCTACAAACGGAATGGCTGAGCTACCAACTGACCACTTTCCGCC
AAGACTTCTGTCGTCTTGCGCGTGAATCTGTAAAAATCATCGTTGATCAAATCAACAACAAAAACAGCAGCTTGGTAAAG
ATGATGGTGCCAGTCAAACTGATAGAGCGCAGCACTACCAAAGCCTAG

Upstream 100 bases:

>100_bases
CAACCCCTTACTTACCTAAATCTTACTTATTTCTCCTTATGACTACGTACCTATTTATGTTAACCTAGCCACAATTAAAC
TGACTTATGAATCCGATCAC

Downstream 100 bases:

>100_bases
TTCCAGAGAAGCCAACCCAAGCGGTTGGCTTTTTTATTTCCTCACTTCTTCTGTCACTTATCCCTTACCTCTACTTCTAA
GATTTCGAACCAATGGCTCA

Product: hypothetical protein

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 335; Mature: 335

Protein sequence:

>335_residues
MNPAKQTITSKDVAKLAGVSQSTVSRVFVPGSSVSEKTKQKVFEAAKALNYRPNAFARSLTTNESKLIGLVFPDADYPIH
MKTLQLISSELQKQGYSAVLIPWQVDDEDNHSIPNIFQYRVDGVIAASATFNKSLYEECEEFNIPIVQYARVVEGTKSSY
VISDNYEAGQQAAQLLHERGVKNAVYLTGEVPTFTNDERQSGFSSEFEDLTGKTPRIVEASYDYASSLDKVRAMLADKNR
PEAVFCATDNLAMAVMDIARIECGIRIPQDLKVIGFDNILQTEWLSYQLTTFRQDFCRLARESVKIIVDQINNKNSSLVK
MMVPVKLIERSTTKA

Sequences:

>Translated_335_residues
MNPAKQTITSKDVAKLAGVSQSTVSRVFVPGSSVSEKTKQKVFEAAKALNYRPNAFARSLTTNESKLIGLVFPDADYPIH
MKTLQLISSELQKQGYSAVLIPWQVDDEDNHSIPNIFQYRVDGVIAASATFNKSLYEECEEFNIPIVQYARVVEGTKSSY
VISDNYEAGQQAAQLLHERGVKNAVYLTGEVPTFTNDERQSGFSSEFEDLTGKTPRIVEASYDYASSLDKVRAMLADKNR
PEAVFCATDNLAMAVMDIARIECGIRIPQDLKVIGFDNILQTEWLSYQLTTFRQDFCRLARESVKIIVDQINNKNSSLVK
MMVPVKLIERSTTKA
>Mature_335_residues
MNPAKQTITSKDVAKLAGVSQSTVSRVFVPGSSVSEKTKQKVFEAAKALNYRPNAFARSLTTNESKLIGLVFPDADYPIH
MKTLQLISSELQKQGYSAVLIPWQVDDEDNHSIPNIFQYRVDGVIAASATFNKSLYEECEEFNIPIVQYARVVEGTKSSY
VISDNYEAGQQAAQLLHERGVKNAVYLTGEVPTFTNDERQSGFSSEFEDLTGKTPRIVEASYDYASSLDKVRAMLADKNR
PEAVFCATDNLAMAVMDIARIECGIRIPQDLKVIGFDNILQTEWLSYQLTTFRQDFCRLARESVKIIVDQINNKNSSLVK
MMVPVKLIERSTTKA

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787948, Length=329, Percent_Identity=31.0030395136778, Blast_Score=152, Evalue=3e-38,
Organism=Escherichia coli, GI1790194, Length=328, Percent_Identity=27.7439024390244, Blast_Score=128, Evalue=5e-31,
Organism=Escherichia coli, GI1787580, Length=288, Percent_Identity=28.8194444444444, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI1788474, Length=307, Percent_Identity=29.9674267100977, Blast_Score=111, Evalue=5e-26,
Organism=Escherichia coli, GI1790369, Length=334, Percent_Identity=26.9461077844311, Blast_Score=111, Evalue=7e-26,
Organism=Escherichia coli, GI1786540, Length=333, Percent_Identity=27.3273273273273, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI48994940, Length=278, Percent_Identity=26.978417266187, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1789068, Length=277, Percent_Identity=26.3537906137184, Blast_Score=92, Evalue=7e-20,
Organism=Escherichia coli, GI1786268, Length=278, Percent_Identity=27.3381294964029, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1789202, Length=309, Percent_Identity=23.3009708737864, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI1790715, Length=315, Percent_Identity=20.952380952381, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1787906, Length=262, Percent_Identity=29.0076335877863, Blast_Score=81, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 37437; Mature: 37437

Theoretical pI: Translated: 5.87; Mature: 5.87

Prosite motif: PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPAKQTITSKDVAKLAGVSQSTVSRVFVPGSSVSEKTKQKVFEAAKALNYRPNAFARSL
CCCHHHHHHHHHHHHHHCCCHHHHHEEEECCCCHHHHHHHHHHHHHHHCCCCCCHHHHHC
TTNESKLIGLVFPDADYPIHMKTLQLISSELQKQGYSAVLIPWQVDDEDNHSIPNIFQYR
CCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCHHHHHH
VDGVIAASATFNKSLYEECEEFNIPIVQYARVVEGTKSSYVISDNYEAGQQAAQLLHERG
HCEEEEECHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHCC
VKNAVYLTGEVPTFTNDERQSGFSSEFEDLTGKTPRIVEASYDYASSLDKVRAMLADKNR
CCCEEEEECCCCCCCCCHHHCCCCHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHCCCC
PEAVFCATDNLAMAVMDIARIECGIRIPQDLKVIGFDNILQTEWLSYQLTTFRQDFCRLA
CCEEEEECCCHHHHHHHHHHHHCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHH
RESVKIIVDQINNKNSSLVKMMVPVKLIERSTTKA
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MNPAKQTITSKDVAKLAGVSQSTVSRVFVPGSSVSEKTKQKVFEAAKALNYRPNAFARSL
CCCHHHHHHHHHHHHHHCCCHHHHHEEEECCCCHHHHHHHHHHHHHHHCCCCCCHHHHHC
TTNESKLIGLVFPDADYPIHMKTLQLISSELQKQGYSAVLIPWQVDDEDNHSIPNIFQYR
CCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCHHHHHH
VDGVIAASATFNKSLYEECEEFNIPIVQYARVVEGTKSSYVISDNYEAGQQAAQLLHERG
HCEEEEECHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHCC
VKNAVYLTGEVPTFTNDERQSGFSSEFEDLTGKTPRIVEASYDYASSLDKVRAMLADKNR
CCCEEEEECCCCCCCCCHHHCCCCHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHCCCC
PEAVFCATDNLAMAVMDIARIECGIRIPQDLKVIGFDNILQTEWLSYQLTTFRQDFCRLA
CCEEEEECCCHHHHHHHHHHHHCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHH
RESVKIIVDQINNKNSSLVKMMVPVKLIERSTTKA
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA