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The map label for this gene is ompF [H]

Identifier: 156976043

GI number: 156976043

Start: 74672

End: 75769

Strand: Reverse

Name: ompF [H]

Synonym: VIBHAR_04813

Alternate gene names: 156976043

Gene position: 75769-74672 (Counterclockwise)

Preceding gene: 156976044

Following gene: 156976042

Centisome position: 3.44

GC content: 48.91

Gene sequence:

>1098_bases
ATGAGATTGATGATGAAAAAGTCGACCCTTTCTGCAGTGATTTTAGCAGCGCTTACTTCTAGCTCAGCCTTTGCTGCACA
CACTTTTATTAATGACGCTGGCGACAGCCTAACCATCGATGGCCGTTTCGATCTTCGCTACCAAGACCGTGGCGGTGATG
ACAACGGCGAATGGAACAGCGGCAGCTCACGTTTCGGTTTGAAAGGCCAAACGGATCTGGATAACGGCTGGACTGGTTTC
GGTCACGCGGAATGGGGCTACAACTCTGGCGCAAACGGCGACAACATTTACGACCGCTTGCTATACGCAGGTGTTGATCA
CGAAAAATACGGCAAGATTGCTGCGGGTACAAAGCAGTGGTCTACCTTCTACGACGTAGCATGGTACACCGATTTAGGTC
GTGTATTCGGTACTCGTGGCTCAGGTGTTTACAACCTAGCTGACTGGGGTATCGCATCCGGTGCTGGTCGTGCAGAAAAC
TCAATTACGTACCGTAATTCAATCAATGAAAAAGTGGGCTATGGCTTTACTTACCAAACCACGCGTGAAGATGTTGCTCT
AGCTAACAATGCAACAGCATCACTGAAAAACGGTATGGGTGCGTCGATCACTTACAAACCGGTTGATGGCGTAACACTTG
GTGCGGCTTACCATCAAAATGAAGTTGCGGATCTAGACGCAAGCGTGGTTGGCGTAGAAAACGGCGACAACATGCGCATC
ATGCTATTGGGCGCGAACTACAGCAACGGTGGTTTCTACGCGGGCGCGACTTTCCACGTTGGTGAAAACTGGGAAGCAGT
AAGCCAAAGCGGTACGGACGTGATGTTCGATACCCTGGGTGGTGAAATCTACACTTACTACCACTTCGACAACGGCCTAC
GCCCAACATTGAACTACAACTACATGGAAGATCGTGGTGACGAGACACAAGGTTACGAGCGTAACTTGCTGATTCCTGGC
CTTGAATATCACTTCCAAAAGAACAAATTCCTAGTTTGGACTGAATACCAGTTCGATCTAGGTAAAGATCAGTACGATGG
CAGCAAATTCGAAAACCGTGATGATCAATTCGCGGCAGGTATTCGCTACTACTTCTGA

Upstream 100 bases:

>100_bases
CATGCTCCCAACTAGTGCCTCAAGGATGGGGCACAACCTCAAGATAGAACCCTTATTCAAAACGGGCATGAACGCACGGT
GGCTTTCTGCGCCCTGAATA

Downstream 100 bases:

>100_bases
TAACCACGAGATAAGTTATTCCGGTTACGTAACTTATTCGATTTAGTGGAAATGGGAAATAAGAGCAAGCCTTACCTTTT
TTGGTAAGGCTTTTTCGTAT

Product: hypothetical protein

Products: NA

Alternate protein names: Outer membrane protein S3; Porin ompF [H]

Number of amino acids: Translated: 365; Mature: 365

Protein sequence:

>365_residues
MRLMMKKSTLSAVILAALTSSSAFAAHTFINDAGDSLTIDGRFDLRYQDRGGDDNGEWNSGSSRFGLKGQTDLDNGWTGF
GHAEWGYNSGANGDNIYDRLLYAGVDHEKYGKIAAGTKQWSTFYDVAWYTDLGRVFGTRGSGVYNLADWGIASGAGRAEN
SITYRNSINEKVGYGFTYQTTREDVALANNATASLKNGMGASITYKPVDGVTLGAAYHQNEVADLDASVVGVENGDNMRI
MLLGANYSNGGFYAGATFHVGENWEAVSQSGTDVMFDTLGGEIYTYYHFDNGLRPTLNYNYMEDRGDETQGYERNLLIPG
LEYHFQKNKFLVWTEYQFDLGKDQYDGSKFENRDDQFAAGIRYYF

Sequences:

>Translated_365_residues
MRLMMKKSTLSAVILAALTSSSAFAAHTFINDAGDSLTIDGRFDLRYQDRGGDDNGEWNSGSSRFGLKGQTDLDNGWTGF
GHAEWGYNSGANGDNIYDRLLYAGVDHEKYGKIAAGTKQWSTFYDVAWYTDLGRVFGTRGSGVYNLADWGIASGAGRAEN
SITYRNSINEKVGYGFTYQTTREDVALANNATASLKNGMGASITYKPVDGVTLGAAYHQNEVADLDASVVGVENGDNMRI
MLLGANYSNGGFYAGATFHVGENWEAVSQSGTDVMFDTLGGEIYTYYHFDNGLRPTLNYNYMEDRGDETQGYERNLLIPG
LEYHFQKNKFLVWTEYQFDLGKDQYDGSKFENRDDQFAAGIRYYF
>Mature_365_residues
MRLMMKKSTLSAVILAALTSSSAFAAHTFINDAGDSLTIDGRFDLRYQDRGGDDNGEWNSGSSRFGLKGQTDLDNGWTGF
GHAEWGYNSGANGDNIYDRLLYAGVDHEKYGKIAAGTKQWSTFYDVAWYTDLGRVFGTRGSGVYNLADWGIASGAGRAEN
SITYRNSINEKVGYGFTYQTTREDVALANNATASLKNGMGASITYKPVDGVTLGAAYHQNEVADLDASVVGVENGDNMRI
MLLGANYSNGGFYAGATFHVGENWEAVSQSGTDVMFDTLGGEIYTYYHFDNGLRPTLNYNYMEDRGDETQGYERNLLIPG
LEYHFQKNKFLVWTEYQFDLGKDQYDGSKFENRDDQFAAGIRYYF

Specific function: OmpF is a porin that forms passive diffusion pores which allow small molecular weight hydrophilic materials across the outer membrane. It is also a receptor for the bacteriophage T2 [H]

COG id: COG3203

COG function: function code M; Outer membrane protein (porin)

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Gram-negative porin family [H]

Homologues:

Organism=Escherichia coli, GI1788544, Length=387, Percent_Identity=27.906976744186, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1787160, Length=386, Percent_Identity=26.6839378238342, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1787641, Length=332, Percent_Identity=25.9036144578313, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1786436, Length=386, Percent_Identity=28.2383419689119, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: 6020 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 19068 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001897
- InterPro:   IPR013793
- InterPro:   IPR001702 [H]

Pfam domain/function: PF00267 Porin_1 [H]

EC number: NA

Molecular weight: Translated: 40443; Mature: 40443

Theoretical pI: Translated: 4.55; Mature: 4.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLMMKKSTLSAVILAALTSSSAFAAHTFINDAGDSLTIDGRFDLRYQDRGGDDNGEWNS
CCEEECHHHHHHHHHHHHCCCCCEEEEEEECCCCCEEEECCEEEEEECCCCCCCCCCCCC
GSSRFGLKGQTDLDNGWTGFGHAEWGYNSGANGDNIYDRLLYAGVDHEKYGKIAAGTKQW
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCHHHCCCEECCCCCC
STFYDVAWYTDLGRVFGTRGSGVYNLADWGIASGAGRAENSITYRNSINEKVGYGFTYQT
CEEEEEHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCEEECCCCCHHHCCCEEEEC
TREDVALANNATASLKNGMGASITYKPVDGVTLGAAYHQNEVADLDASVVGVENGDNMRI
CCCCEEECCCCCHHHHCCCCCEEEECCCCCEEEECCCCCCCCCCCCEEEEEEECCCCEEE
MLLGANYSNGGFYAGATFHVGENWEAVSQSGTDVMFDTLGGEIYTYYHFDNGLRPTLNYN
EEEECCCCCCCEEEEEEEEECCCCHHHHCCCCEEEEEECCCEEEEEEEECCCCCCCCCCC
YMEDRGDETQGYERNLLIPGLEYHFQKNKFLVWTEYQFDLGKDQYDGSKFENRDDQFAAG
CHHCCCCCCCCCCCEEEECCHHHEECCCEEEEEEEEEECCCCCCCCCCCCCCCCCHHCCC
IRYYF
EEECC
>Mature Secondary Structure
MRLMMKKSTLSAVILAALTSSSAFAAHTFINDAGDSLTIDGRFDLRYQDRGGDDNGEWNS
CCEEECHHHHHHHHHHHHCCCCCEEEEEEECCCCCEEEECCEEEEEECCCCCCCCCCCCC
GSSRFGLKGQTDLDNGWTGFGHAEWGYNSGANGDNIYDRLLYAGVDHEKYGKIAAGTKQW
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCHHHCCCEECCCCCC
STFYDVAWYTDLGRVFGTRGSGVYNLADWGIASGAGRAENSITYRNSINEKVGYGFTYQT
CEEEEEHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCEEECCCCCHHHCCCEEEEC
TREDVALANNATASLKNGMGASITYKPVDGVTLGAAYHQNEVADLDASVVGVENGDNMRI
CCCCEEECCCCCHHHHCCCCCEEEECCCCCEEEECCCCCCCCCCCCEEEEEEECCCCEEE
MLLGANYSNGGFYAGATFHVGENWEAVSQSGTDVMFDTLGGEIYTYYHFDNGLRPTLNYN
EEEECCCCCCCEEEEEEEEECCCCHHHHCCCCEEEEEECCCEEEEEEEECCCCCCCCCCC
YMEDRGDETQGYERNLLIPGLEYHFQKNKFLVWTEYQFDLGKDQYDGSKFENRDDQFAAG
CHHCCCCCCCCCCCEEEECCHHHEECCCEEEEEEEEEECCCCCCCCCCCCCCCCCHHCCC
IRYYF
EEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]