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The map label for this gene is tfb

Identifier: 156938159

GI number: 156938159

Start: 1227915

End: 1228865

Strand: Reverse

Name: tfb

Synonym: Igni_1372

Alternate gene names: 156938159

Gene position: 1228865-1227915 (Counterclockwise)

Preceding gene: 156938160

Following gene: 156938157

Centisome position: 94.71

GC content: 55.52

Gene sequence:

>951_bases
TTGCCCGAACAGGAGGCTTTTAGGCTCCGTTGCCCCGTGTGCGGGAGCACCGACATAGTCTTCAACGAGGAGACCGGGGA
GTACGTGTGCGCCCGTTGCGGTACCATAGTGCTGGACCGGTACGTGGACCAAGGCCCCGAGTGGAGGGCCTTCACCCCCG
AGGAGAGGGAAAGGAGGGGTAGGACCGGCGCCCCCCTCTCTCCCACCCTCCACGACCACGGCTTGTCCACGGTTATAGAT
CATAGGGATCGGGACGCGTTAGGGAAGCGCCTAAGCCCTAGGAAGAGGCAAGAAGTCCAGAGGCTTAGGAAGTGGCAGCT
CAGGGCGCGCATCCAGACGGGTATGGATAGGAACCTAACTATAGCTATGAACGAGCTCGACAGGATGGCAAACCTCTTGA
ACCTCCCGAAACAGATCAAGGAAGAGGCGGCGGTAATCTATAGGAAGGCCGTGGAGAAGGGCCTCGTTAGGGGGAGGAGC
ATAGAGTCCGTAGTAGCGGCTGTAATATATGCCGCTTGTAGGATCCACCACCAACCGCGCACCTTGGACGAGATAGCTAA
GAAGTTGGAGGTGAATAGGAAAGAAGTGGCCAGGTGTTACAGGCTTATAACTAAAGAGCTCAAACTAAAGGTGCCCATCG
CCGACGCAATGGACCACATACCCAGAATAGGCGAGGCCCTCAAGCTCAGGGGCGATATAATAGAATACGCCATGAAGATC
ATGGAAAAGATAAAGGGGCACCCGATAACCGCCGGAAAGGACCCCGCGGGCATAGCGGCGGCAGTGATATACATAGCTGT
CATGCAGAAGGGCGAAAGGAGGACGCAGAAGGAGATAGCGAACGTTGCCGGAGTAACCGAGGTCACTGTGAGAAACAGGT
ACAAGGAGATAATGAAGGTCCTCAACGAGATGGACTTAGAGGAGATCGAGAAAGAGGTCTCAAAGAAGTAG

Upstream 100 bases:

>100_bases
CTCCTATCCTTCGCTTAGAGATAGTACACCTCATGCGTAAAATTTATAAATATCTAGGGTCGTGTAATCAACGAAACGAG
TAAAAGGGGTGTGGGCTAGG

Downstream 100 bases:

>100_bases
CTAACTCTATGAGGGCCCTCGCGGCGTAGACCGCGCTGACCCCGGAGGGGTCGTAGGGCGGGGAGAGCTCCACCACGTCC
GCGCCGGCTAAGGGCAAGTG

Product: transcription initiation factor IIB

Products: NA

Alternate protein names: TFIIB

Number of amino acids: Translated: 316; Mature: 315

Protein sequence:

>316_residues
MPEQEAFRLRCPVCGSTDIVFNEETGEYVCARCGTIVLDRYVDQGPEWRAFTPEERERRGRTGAPLSPTLHDHGLSTVID
HRDRDALGKRLSPRKRQEVQRLRKWQLRARIQTGMDRNLTIAMNELDRMANLLNLPKQIKEEAAVIYRKAVEKGLVRGRS
IESVVAAVIYAACRIHHQPRTLDEIAKKLEVNRKEVARCYRLITKELKLKVPIADAMDHIPRIGEALKLRGDIIEYAMKI
MEKIKGHPITAGKDPAGIAAAVIYIAVMQKGERRTQKEIANVAGVTEVTVRNRYKEIMKVLNEMDLEEIEKEVSKK

Sequences:

>Translated_316_residues
MPEQEAFRLRCPVCGSTDIVFNEETGEYVCARCGTIVLDRYVDQGPEWRAFTPEERERRGRTGAPLSPTLHDHGLSTVID
HRDRDALGKRLSPRKRQEVQRLRKWQLRARIQTGMDRNLTIAMNELDRMANLLNLPKQIKEEAAVIYRKAVEKGLVRGRS
IESVVAAVIYAACRIHHQPRTLDEIAKKLEVNRKEVARCYRLITKELKLKVPIADAMDHIPRIGEALKLRGDIIEYAMKI
MEKIKGHPITAGKDPAGIAAAVIYIAVMQKGERRTQKEIANVAGVTEVTVRNRYKEIMKVLNEMDLEEIEKEVSKK
>Mature_315_residues
PEQEAFRLRCPVCGSTDIVFNEETGEYVCARCGTIVLDRYVDQGPEWRAFTPEERERRGRTGAPLSPTLHDHGLSTVIDH
RDRDALGKRLSPRKRQEVQRLRKWQLRARIQTGMDRNLTIAMNELDRMANLLNLPKQIKEEAAVIYRKAVEKGLVRGRSI
ESVVAAVIYAACRIHHQPRTLDEIAKKLEVNRKEVARCYRLITKELKLKVPIADAMDHIPRIGEALKLRGDIIEYAMKIM
EKIKGHPITAGKDPAGIAAAVIYIAVMQKGERRTQKEIANVAGVTEVTVRNRYKEIMKVLNEMDLEEIEKEVSKK

Specific function: Stabilizes TBP binding to an archaeal box-A promoter. Also responsible for recruiting RNA polymerase II to the pre- initiation complex (DNA-TBP-TFIIB)

COG id: COG1405

COG function: function code K; Transcription initiation factor TFIIIB, Brf1 subunit/Transcription initiation factor TFIIB

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TFIIB-type zinc finger

Homologues:

Organism=Homo sapiens, GI4504193, Length=291, Percent_Identity=32.6460481099656, Blast_Score=160, Evalue=1e-39,
Organism=Homo sapiens, GI22035556, Length=294, Percent_Identity=25.5102040816327, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17564892, Length=291, Percent_Identity=33.6769759450172, Blast_Score=164, Evalue=7e-41,
Organism=Caenorhabditis elegans, GI17531397, Length=294, Percent_Identity=22.4489795918367, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6325343, Length=303, Percent_Identity=26.7326732673267, Blast_Score=95, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6321685, Length=169, Percent_Identity=28.9940828402367, Blast_Score=79, Evalue=7e-16,
Organism=Drosophila melanogaster, GI17136758, Length=275, Percent_Identity=34.1818181818182, Blast_Score=156, Evalue=1e-38,
Organism=Drosophila melanogaster, GI281361961, Length=296, Percent_Identity=22.2972972972973, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24647658, Length=296, Percent_Identity=22.2972972972973, Blast_Score=70, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TF2B_IGNH4 (A8AC96)

Other databases:

- EMBL:   CP000816
- RefSeq:   YP_001435955.1
- ProteinModelPortal:   A8AC96
- STRING:   A8AC96
- GeneID:   5562291
- GenomeReviews:   CP000816_GR
- KEGG:   iho:Igni_1372
- eggNOG:   arNOG04485
- HOGENOM:   HBG646536
- OMA:   TRTYRYV
- BioCyc:   IHOS453591:IGNI_1372-MONOMER
- HAMAP:   MF_00383
- InterPro:   IPR006670
- InterPro:   IPR011028
- InterPro:   IPR013763
- InterPro:   IPR007630
- InterPro:   IPR000812
- InterPro:   IPR013150
- InterPro:   IPR013137
- Gene3D:   G3DSA:1.10.472.10
- PANTHER:   PTHR11618
- PRINTS:   PR00685
- SMART:   SM00385

Pfam domain/function: PF04545 Sigma70_r4; PF00382 TFIIB; PF08271 TFIIB_Zn_Ribbon; SSF47954 Cyclin_like

EC number: NA

Molecular weight: Translated: 36095; Mature: 35963

Theoretical pI: Translated: 9.93; Mature: 9.93

Prosite motif: PS00782 TFIIB; PS51134 ZF_TFIIB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEQEAFRLRCPVCGSTDIVFNEETGEYVCARCGTIVLDRYVDQGPEWRAFTPEERERRG
CCCCCCCEEECCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHCC
RTGAPLSPTLHDHGLSTVIDHRDRDALGKRLSPRKRQEVQRLRKWQLRARIQTGMDRNLT
CCCCCCCCCHHHCCHHHHHHCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCE
IAMNELDRMANLLNLPKQIKEEAAVIYRKAVEKGLVRGRSIESVVAAVIYAACRIHHQPR
EHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCC
TLDEIAKKLEVNRKEVARCYRLITKELKLKVPIADAMDHIPRIGEALKLRGDIIEYAMKI
CHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MEKIKGHPITAGKDPAGIAAAVIYIAVMQKGERRTQKEIANVAGVTEVTVRNRYKEIMKV
HHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHH
LNEMDLEEIEKEVSKK
HHHCCHHHHHHHHCCC
>Mature Secondary Structure 
PEQEAFRLRCPVCGSTDIVFNEETGEYVCARCGTIVLDRYVDQGPEWRAFTPEERERRG
CCCCCCEEECCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHCC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HHHCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA