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The map label for this gene is hemL [H]

Identifier: 154686948

GI number: 154686948

Start: 2637451

End: 2638740

Strand: Reverse

Name: hemL [H]

Synonym: RBAM_025180

Alternate gene names: 154686948

Gene position: 2638740-2637451 (Counterclockwise)

Preceding gene: 154686949

Following gene: 154686947

Centisome position: 67.34

GC content: 50.47

Gene sequence:

>1290_bases
ATGAGAAGCTATGAAAAATCAAAAGCCGCTTTTAAAGAAGCGCAGAAACTGATGCCGGGCGGAGTGAACAGCCCAGTGCG
CGCGTTTAAATCAGTCGATATGGACCCGATCTTTATGCAGCGCGGAAAAGGATCGAAAATTTTTGATATTGACGGCAATG
AATATATCGACTACGTCCTGAGCTGGGGGCCGCTCATTTTGGGCCACACGAACGACCGGGTCGTTGAAAGCCTGAAAAAA
GTAGCCGAGAACGGGACAAGTTTCGGGGCATCTACTGAAGTGGAAAACGAACTGGCCAAATTGGTCATTGACCGTGTGCC
GTCTGTTGAAATCGTGCGGATGGTCAGCTCCGGCACAGAGGCGACAATGAGCGCCCTGCGTCTGGCAAGAGGATATACCG
GCCGAAACAAAATTTTGAAATTCGAAGGCTGTTACCACGGCCATGGTGATTCACTGCTGATCAAAGCGGGATCCGGAGTG
GCGACACTCGGCCTTCCGGACAGCCCGGGCGTGCCGGAAGGAATAGCAATGAATACGATTACCGTTCCATACAACGACTT
GGAAAGCGTTGCACTCGCATTCCGGGAGTACGGTGAAGATATTGCCGGGGTCATTGTTGAACCGGTAGCAGGCAATATGG
GCGTTGTTCCGCCGCAGGAAGGCTTTTTACAAGGACTTCGCGACATGACGGAACAGTATGGCGCGCTGCTCATTTTTGAT
GAGGTCATGACCGGTTTTCGCGTGGATTATAACTGTGCGCAAGGGTATTTCGGCGTGACGCCTGACTTAACGTGTCTCGG
CAAAGTCATCGGCGGCGGTCTTCCTGTCGGTGCGTATGGCGGAAAAGCTGACATTATGGAACGAATCGCGCCGAGCGGTC
CGATCTATCAGGCTGGAACATTGTCAGGAAATCCGCTTGCCATGACAGCGGGACTTGAGACGTTACAGCAGCTCACACCT
GAATCTTACCAAGAATTTGTGAAAAAAGGCGATATGCTTGAAGAAGGCATTTCAAAAGCAGCTGCAGCACATGGCATTCC
GCACACGTTTAACCGGGCGGGTTCAATGATCGGCTTCTTTTTCACAAATGAGCCTGTCATTAACTATGAAGCAGCCAAAT
CATCTGATTTAAAGCTGTTCGCTAAATATTATAAAGGGATGGCTGACGCAGGCGTATTTCTGCCGCCGTCTCAATTTGAA
GGATTGTTTCTCTCCACTGCGCATACAATGGAGGATATTGAAAATACCATTCAAGCCGCTGAGAAAGTGTTCCAGGCGAT
CAGCCGCTGA

Upstream 100 bases:

>100_bases
ATGAAACGTGCAGGAGCCGATCTGATTATGACGTATCATGCAAAAGACGCAGCAAAATGGCTTGCTGAGTAACGATTTCA
TTTGACGGGAGCTGAGGCAG

Downstream 100 bases:

>100_bases
TACAGAAAAGCCGATGCAAGCAAGACTTGTATCGGCTTTTTTCAATGCCTCCATTATCATATTTTTCCGGATTGCGGCAT
ACACCTTTAGTGACATCTAA

Product: glutamate-1-semialdehyde aminotransferase

Products: NA

Alternate protein names: GSA; Glutamate-1-semialdehyde aminotransferase; GSA-AT [H]

Number of amino acids: Translated: 429; Mature: 429

Protein sequence:

>429_residues
MRSYEKSKAAFKEAQKLMPGGVNSPVRAFKSVDMDPIFMQRGKGSKIFDIDGNEYIDYVLSWGPLILGHTNDRVVESLKK
VAENGTSFGASTEVENELAKLVIDRVPSVEIVRMVSSGTEATMSALRLARGYTGRNKILKFEGCYHGHGDSLLIKAGSGV
ATLGLPDSPGVPEGIAMNTITVPYNDLESVALAFREYGEDIAGVIVEPVAGNMGVVPPQEGFLQGLRDMTEQYGALLIFD
EVMTGFRVDYNCAQGYFGVTPDLTCLGKVIGGGLPVGAYGGKADIMERIAPSGPIYQAGTLSGNPLAMTAGLETLQQLTP
ESYQEFVKKGDMLEEGISKAAAAHGIPHTFNRAGSMIGFFFTNEPVINYEAAKSSDLKLFAKYYKGMADAGVFLPPSQFE
GLFLSTAHTMEDIENTIQAAEKVFQAISR

Sequences:

>Translated_429_residues
MRSYEKSKAAFKEAQKLMPGGVNSPVRAFKSVDMDPIFMQRGKGSKIFDIDGNEYIDYVLSWGPLILGHTNDRVVESLKK
VAENGTSFGASTEVENELAKLVIDRVPSVEIVRMVSSGTEATMSALRLARGYTGRNKILKFEGCYHGHGDSLLIKAGSGV
ATLGLPDSPGVPEGIAMNTITVPYNDLESVALAFREYGEDIAGVIVEPVAGNMGVVPPQEGFLQGLRDMTEQYGALLIFD
EVMTGFRVDYNCAQGYFGVTPDLTCLGKVIGGGLPVGAYGGKADIMERIAPSGPIYQAGTLSGNPLAMTAGLETLQQLTP
ESYQEFVKKGDMLEEGISKAAAAHGIPHTFNRAGSMIGFFFTNEPVINYEAAKSSDLKLFAKYYKGMADAGVFLPPSQFE
GLFLSTAHTMEDIENTIQAAEKVFQAISR
>Mature_429_residues
MRSYEKSKAAFKEAQKLMPGGVNSPVRAFKSVDMDPIFMQRGKGSKIFDIDGNEYIDYVLSWGPLILGHTNDRVVESLKK
VAENGTSFGASTEVENELAKLVIDRVPSVEIVRMVSSGTEATMSALRLARGYTGRNKILKFEGCYHGHGDSLLIKAGSGV
ATLGLPDSPGVPEGIAMNTITVPYNDLESVALAFREYGEDIAGVIVEPVAGNMGVVPPQEGFLQGLRDMTEQYGALLIFD
EVMTGFRVDYNCAQGYFGVTPDLTCLGKVIGGGLPVGAYGGKADIMERIAPSGPIYQAGTLSGNPLAMTAGLETLQQLTP
ESYQEFVKKGDMLEEGISKAAAAHGIPHTFNRAGSMIGFFFTNEPVINYEAAKSSDLKLFAKYYKGMADAGVFLPPSQFE
GLFLSTAHTMEDIENTIQAAEKVFQAISR

Specific function: Porphyrin biosynthesis by the C5 pathway; second step. [C]

COG id: COG0001

COG function: function code H; Glutamate-1-semialdehyde aminotransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557809, Length=307, Percent_Identity=30.6188925081433, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI24119277, Length=343, Percent_Identity=25.9475218658892, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI284507298, Length=226, Percent_Identity=32.7433628318584, Blast_Score=90, Evalue=4e-18,
Organism=Escherichia coli, GI1786349, Length=424, Percent_Identity=54.7169811320755, Blast_Score=482, Evalue=1e-137,
Organism=Escherichia coli, GI1789016, Length=381, Percent_Identity=29.9212598425197, Blast_Score=139, Evalue=4e-34,
Organism=Escherichia coli, GI1788044, Length=361, Percent_Identity=31.8559556786704, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI1789759, Length=278, Percent_Identity=33.4532374100719, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI145693181, Length=358, Percent_Identity=29.608938547486, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1787560, Length=360, Percent_Identity=28.8888888888889, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1786991, Length=347, Percent_Identity=26.8011527377522, Blast_Score=80, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI25144271, Length=302, Percent_Identity=28.8079470198675, Blast_Score=112, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI71992977, Length=344, Percent_Identity=25.8720930232558, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI25144274, Length=147, Percent_Identity=36.734693877551, Blast_Score=88, Evalue=7e-18,
Organism=Saccharomyces cerevisiae, GI6323470, Length=303, Percent_Identity=28.3828382838284, Blast_Score=103, Evalue=6e-23,
Organism=Saccharomyces cerevisiae, GI6324432, Length=354, Percent_Identity=27.1186440677966, Blast_Score=96, Evalue=1e-20,
Organism=Drosophila melanogaster, GI21357415, Length=322, Percent_Identity=26.7080745341615, Blast_Score=98, Evalue=8e-21,
Organism=Drosophila melanogaster, GI21356575, Length=329, Percent_Identity=26.7477203647416, Blast_Score=92, Evalue=8e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004639
- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00202 Aminotran_3 [H]

EC number: =5.4.3.8 [H]

Molecular weight: Translated: 46139; Mature: 46139

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSYEKSKAAFKEAQKLMPGGVNSPVRAFKSVDMDPIFMQRGKGSKIFDIDGNEYIDYVL
CCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCHHHHCCCCCCEEEECCCCHHHHHHH
SWGPLILGHTNDRVVESLKKVAENGTSFGASTEVENELAKLVIDRVPSVEIVRMVSSGTE
HCCCEEEECCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCHH
ATMSALRLARGYTGRNKILKFEGCYHGHGDSLLIKAGSGVATLGLPDSPGVPEGIAMNTI
HHHHHHHHHHCCCCCCCEEEEECCCCCCCCEEEEECCCCEEEECCCCCCCCCCCCEEEEE
TVPYNDLESVALAFREYGEDIAGVIVEPVAGNMGVVPPQEGFLQGLRDMTEQYGALLIFD
ECCCCHHHHHHHHHHHHCCHHHHHEEECCCCCCCCCCCHHHHHHHHHHHHHHHCEEEEEH
EVMTGFRVDYNCAQGYFGVTPDLTCLGKVIGGGLPVGAYGGKADIMERIAPSGPIYQAGT
HHHHCCEECEECCCCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCEECCC
LSGNPLAMTAGLETLQQLTPESYQEFVKKGDMLEEGISKAAAAHGIPHTFNRAGSMIGFF
CCCCCEEEHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHCCCEEEEE
FTNEPVINYEAAKSSDLKLFAKYYKGMADAGVFLPPSQFEGLFLSTAHTMEDIENTIQAA
ECCCCEEEECCCCCCCHHHHHHHHCCHHCCCCCCCCHHCCCEEEHHHHHHHHHHHHHHHH
EKVFQAISR
HHHHHHHCC
>Mature Secondary Structure
MRSYEKSKAAFKEAQKLMPGGVNSPVRAFKSVDMDPIFMQRGKGSKIFDIDGNEYIDYVL
CCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCHHHHCCCCCCEEEECCCCHHHHHHH
SWGPLILGHTNDRVVESLKKVAENGTSFGASTEVENELAKLVIDRVPSVEIVRMVSSGTE
HCCCEEEECCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCHH
ATMSALRLARGYTGRNKILKFEGCYHGHGDSLLIKAGSGVATLGLPDSPGVPEGIAMNTI
HHHHHHHHHHCCCCCCCEEEEECCCCCCCCEEEEECCCCEEEECCCCCCCCCCCCEEEEE
TVPYNDLESVALAFREYGEDIAGVIVEPVAGNMGVVPPQEGFLQGLRDMTEQYGALLIFD
ECCCCHHHHHHHHHHHHCCHHHHHEEECCCCCCCCCCCHHHHHHHHHHHHHHHCEEEEEH
EVMTGFRVDYNCAQGYFGVTPDLTCLGKVIGGGLPVGAYGGKADIMERIAPSGPIYQAGT
HHHHCCEECEECCCCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCEECCC
LSGNPLAMTAGLETLQQLTPESYQEFVKKGDMLEEGISKAAAAHGIPHTFNRAGSMIGFF
CCCCCEEEHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHCCCEEEEE
FTNEPVINYEAAKSSDLKLFAKYYKGMADAGVFLPPSQFEGLFLSTAHTMEDIENTIQAA
ECCCCEEEECCCCCCCHHHHHHHHCCHHCCCCCCCCHHCCCEEEHHHHHHHHHHHHHHHH
EKVFQAISR
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1672867; 9384377 [H]