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The map label for this gene is hemB [H]

Identifier: 154686949

GI number: 154686949

Start: 2638769

End: 2639746

Strand: Reverse

Name: hemB [H]

Synonym: RBAM_025190

Alternate gene names: 154686949

Gene position: 2639746-2638769 (Counterclockwise)

Preceding gene: 154686950

Following gene: 154686948

Centisome position: 67.36

GC content: 47.96

Gene sequence:

>978_bases
ATGATGAATCAAACATTTAACAGACACCGCCGTTTGCGCGCGAACGCTGCGATGAGAGATTTAGTAAAAGAAACACGTCT
TCACCCCTCAGATTTTATTTACCCGTTATTTGTCGTTGAAGGACTCGAAGGAAAAAAAGAAGTTCCTTCCATGCCGGGGG
TTTATCATGTATCACTCGATTTGTTAAAGGATGAAATGGCTGAGCTGATATCGCTCGGCATTAAGTCGGTCATCGTTTTC
GGCGTGCCGGAAGAAAAAGACGCCTGCGGAACCCAGGCTTTTCACGATCACGGCATTGTGCAGAAAGGGATTGCTGAAAT
TAAAAAGCATTATCCTGAAATGATCGTCATCGCTGATACGTGTCTCTGTGAATACACGGATCACGGCCATTGCGGGGTCG
TGAAAGACGGAGAAATCCTCAATGACGAGTCGCTTGAACTGCTGGCGCAGACAGCCGTCAGCCAGGCCAAAGCCGGCGCT
GACATCATCGCTCCTTCAAATATGATGGACGGTTTTGTCACAGTGATCAGGGAAGCGCTCGATAAAGAAGGATTCACGAA
TATTCCGATCATGTCTTATGCCGTGAAATATTCAAGTGAATTTTACGGTCCGTTCCGCGACGCGGCTGACAGCACGCCGC
AGTTCGGAGACCGAAAAACATATCAGATGGATCCGGCCAACCGCTTGGAAGCGCTCCGTGAGGCTGAATCAGACGCGGCA
GAAGGCGCTGACTTTTTAATCGTCAAACCGTCTATGTCATACTTAGATATTATGCGTGATGTGAAAAACCGCCATCACCT
GCCGCTGGTCGCATATAATGTGAGCGGGGAGTATTCCATGGTGAAAGCGGCAGCCCAAAACGGCTGGATTAAAGAAAAAG
AAATGGTGCTGGAAATTTTAACGAGCATGAAACGTGCAGGAGCCGATCTGATTATGACGTATCATGCAAAAGACGCAGCA
AAATGGCTTGCTGAGTAA

Upstream 100 bases:

>100_bases
AAGCGCTCTTGGAATACGGGATCTCATCCCATGTGCCTGACACTTATACGATTGAAGGCATGCTGGACGTCATGTGCAGC
ATGTCGGGAAAGGATTGATA

Downstream 100 bases:

>100_bases
CGATTTCATTTGACGGGAGCTGAGGCAGATGAGAAGCTATGAAAAATCAAAAGCCGCTTTTAAAGAAGCGCAGAAACTGA
TGCCGGGCGGAGTGAACAGC

Product: delta-aminolevulinic acid dehydratase

Products: NA

Alternate protein names: ALAD; ALADH; Porphobilinogen synthase [H]

Number of amino acids: Translated: 325; Mature: 325

Protein sequence:

>325_residues
MMNQTFNRHRRLRANAAMRDLVKETRLHPSDFIYPLFVVEGLEGKKEVPSMPGVYHVSLDLLKDEMAELISLGIKSVIVF
GVPEEKDACGTQAFHDHGIVQKGIAEIKKHYPEMIVIADTCLCEYTDHGHCGVVKDGEILNDESLELLAQTAVSQAKAGA
DIIAPSNMMDGFVTVIREALDKEGFTNIPIMSYAVKYSSEFYGPFRDAADSTPQFGDRKTYQMDPANRLEALREAESDAA
EGADFLIVKPSMSYLDIMRDVKNRHHLPLVAYNVSGEYSMVKAAAQNGWIKEKEMVLEILTSMKRAGADLIMTYHAKDAA
KWLAE

Sequences:

>Translated_325_residues
MMNQTFNRHRRLRANAAMRDLVKETRLHPSDFIYPLFVVEGLEGKKEVPSMPGVYHVSLDLLKDEMAELISLGIKSVIVF
GVPEEKDACGTQAFHDHGIVQKGIAEIKKHYPEMIVIADTCLCEYTDHGHCGVVKDGEILNDESLELLAQTAVSQAKAGA
DIIAPSNMMDGFVTVIREALDKEGFTNIPIMSYAVKYSSEFYGPFRDAADSTPQFGDRKTYQMDPANRLEALREAESDAA
EGADFLIVKPSMSYLDIMRDVKNRHHLPLVAYNVSGEYSMVKAAAQNGWIKEKEMVLEILTSMKRAGADLIMTYHAKDAA
KWLAE
>Mature_325_residues
MMNQTFNRHRRLRANAAMRDLVKETRLHPSDFIYPLFVVEGLEGKKEVPSMPGVYHVSLDLLKDEMAELISLGIKSVIVF
GVPEEKDACGTQAFHDHGIVQKGIAEIKKHYPEMIVIADTCLCEYTDHGHCGVVKDGEILNDESLELLAQTAVSQAKAGA
DIIAPSNMMDGFVTVIREALDKEGFTNIPIMSYAVKYSSEFYGPFRDAADSTPQFGDRKTYQMDPANRLEALREAESDAA
EGADFLIVKPSMSYLDIMRDVKNRHHLPLVAYNVSGEYSMVKAAAQNGWIKEKEMVLEILTSMKRAGADLIMTYHAKDAA
KWLAE

Specific function: Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen [H]

COG id: COG0113

COG function: function code H; Delta-aminolevulinic acid dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ALADH family [H]

Homologues:

Organism=Homo sapiens, GI189083849, Length=306, Percent_Identity=44.4444444444444, Blast_Score=271, Evalue=6e-73,
Organism=Escherichia coli, GI87081728, Length=320, Percent_Identity=49.375, Blast_Score=299, Evalue=2e-82,
Organism=Saccharomyces cerevisiae, GI6321398, Length=314, Percent_Identity=44.9044585987261, Blast_Score=258, Evalue=1e-69,
Organism=Drosophila melanogaster, GI21358291, Length=305, Percent_Identity=39.0163934426229, Blast_Score=245, Evalue=3e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001731
- InterPro:   IPR013785 [H]

Pfam domain/function: PF00490 ALAD [H]

EC number: =4.2.1.24 [H]

Molecular weight: Translated: 36251; Mature: 36251

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: PS00169 D_ALA_DEHYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMNQTFNRHRRLRANAAMRDLVKETRLHPSDFIYPLFVVEGLEGKKEVPSMPGVYHVSLD
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHEECCCCCCCCCCCCCEEEHHHH
LLKDEMAELISLGIKSVIVFGVPEEKDACGTQAFHDHGIVQKGIAEIKKHYPEMIVIADT
HHHHHHHHHHHHHHHHEEEECCCCCCCCCCCHHHHHCCHHHHHHHHHHHHCCCEEEEECH
CLCEYTDHGHCGVVKDGEILNDESLELLAQTAVSQAKAGADIIAPSNMMDGFVTVIREAL
HHEECCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHCCCCEECCCHHHHHHHHHHHHHH
DKEGFTNIPIMSYAVKYSSEFYGPFRDAADSTPQFGDRKTYQMDPANRLEALREAESDAA
CCCCCCCCCHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCEECCHHHHHHHHHHHHHHHH
EGADFLIVKPSMSYLDIMRDVKNRHHLPLVAYNVSGEYSMVKAAAQNGWIKEKEMVLEIL
CCCCEEEECCCHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHCCCCHHHHHHHHHH
TSMKRAGADLIMTYHAKDAAKWLAE
HHHHHCCCCEEEEECCHHHHHHHCC
>Mature Secondary Structure
MMNQTFNRHRRLRANAAMRDLVKETRLHPSDFIYPLFVVEGLEGKKEVPSMPGVYHVSLD
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHEECCCCCCCCCCCCCEEEHHHH
LLKDEMAELISLGIKSVIVFGVPEEKDACGTQAFHDHGIVQKGIAEIKKHYPEMIVIADT
HHHHHHHHHHHHHHHHEEEECCCCCCCCCCCHHHHHCCHHHHHHHHHHHHCCCEEEEECH
CLCEYTDHGHCGVVKDGEILNDESLELLAQTAVSQAKAGADIIAPSNMMDGFVTVIREAL
HHEECCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHCCCCEECCCHHHHHHHHHHHHHH
DKEGFTNIPIMSYAVKYSSEFYGPFRDAADSTPQFGDRKTYQMDPANRLEALREAESDAA
CCCCCCCCCHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCEECCHHHHHHHHHHHHHHHH
EGADFLIVKPSMSYLDIMRDVKNRHHLPLVAYNVSGEYSMVKAAAQNGWIKEKEMVLEIL
CCCCEEEECCCHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHCCCCHHHHHHHHHH
TSMKRAGADLIMTYHAKDAAKWLAE
HHHHHCCCCEEEEECCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1672867; 9384377 [H]