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The map label for this gene is punA [H]

Identifier: 154686591

GI number: 154686591

Start: 2249466

End: 2250284

Strand: Reverse

Name: punA [H]

Synonym: RBAM_021600

Alternate gene names: 154686591

Gene position: 2250284-2249466 (Counterclockwise)

Preceding gene: 154686592

Following gene: 154686590

Centisome position: 57.43

GC content: 51.04

Gene sequence:

>819_bases
TTGAAACAAAAGATCGAACGTGCTGCTGCATTTATTAAAGAACACGTGAAAGAAACACCAAAGATCGGTCTGATTTTAGG
GTCAGGGCTCGGCGTATTGGCCGATGAAATTGAAGGAGCTGTCAAGCTTACATATGAAACGATCCCTGATTTTCCGGTCT
CAACGGTAGAAGGCCATGCCGGCCAGCTTGTGATGGGAACGCTTGAAGGTGTGCAGGTCATCGCGATGCAGGGCCGCTTT
CATTACTATGAAGGCTACTCAATGGATCAGGTCACATTTCCCGTCCGCGTGATGAAGGCGCTCGGCGTTGAATCTCTCGT
CGTGACGAATGCAGCCGGCGGTATCAACACTGAATTCCGGGCCGGCGATCTGATGATTATTACAGACCACATCAACTTTA
CGGGCACCAACCCGTTAATCGGGCCGAACGAAGCTGAATTCGGACCGAGATTCCCGGACATGTCAGAAGCCTATGACAAA
GAGCTGTCAGGCTTGGCTGAAAAAACGGCCGATGAGCTGGGCATTCCTGTTCAAAAAGGGGTGTATACAGCTGTTACGGG
GCCGTCTTACGAAACGCCTGCTGAGGTTCGGTTTTTAAGAACGATGGGTTCTGACGCTGTCGGCATGTCGACCGTGCCGG
AAGTCATCGTCGCCAACCACGCGGGGATGCGCGTGCTCGGCATCTCATGCATTTCCAACGCGGCGGCAGGTATTCTTGAC
CAGCCGTTATCCCATGAAGAAGTAATGGAAATGACGGAAAAAGTAAAAGGCGGCTTTTTGAAGCTTGTAAAAGCCGTAGT
CGCACGATCTCAAAAATAA

Upstream 100 bases:

>100_bases
CATTTGCCGATATCGGCGCAACCATTGCGGATAATTTCAAAACGGAAAAGCCGAAATACGGAAAAAGCTTTTTATCAAGA
TTACAGTAGGGGGACTTTTT

Downstream 100 bases:

>100_bases
GTCAGCATAAGTCAGCTGTTTCTTCGGAAGCAGCTGTTTTTTTGTGCCCTCAACACGAAACATTTTCCAGATGAGGCGTA
TAAAACCACCGGATTTGGAA

Product: purine nucleoside phosphorylase

Products: NA

Alternate protein names: Inosine phosphorylase; Purine nucleoside phosphorylase I; PNP I; PU-NPase I [H]

Number of amino acids: Translated: 272; Mature: 272

Protein sequence:

>272_residues
MKQKIERAAAFIKEHVKETPKIGLILGSGLGVLADEIEGAVKLTYETIPDFPVSTVEGHAGQLVMGTLEGVQVIAMQGRF
HYYEGYSMDQVTFPVRVMKALGVESLVVTNAAGGINTEFRAGDLMIITDHINFTGTNPLIGPNEAEFGPRFPDMSEAYDK
ELSGLAEKTADELGIPVQKGVYTAVTGPSYETPAEVRFLRTMGSDAVGMSTVPEVIVANHAGMRVLGISCISNAAAGILD
QPLSHEEVMEMTEKVKGGFLKLVKAVVARSQK

Sequences:

>Translated_272_residues
MKQKIERAAAFIKEHVKETPKIGLILGSGLGVLADEIEGAVKLTYETIPDFPVSTVEGHAGQLVMGTLEGVQVIAMQGRF
HYYEGYSMDQVTFPVRVMKALGVESLVVTNAAGGINTEFRAGDLMIITDHINFTGTNPLIGPNEAEFGPRFPDMSEAYDK
ELSGLAEKTADELGIPVQKGVYTAVTGPSYETPAEVRFLRTMGSDAVGMSTVPEVIVANHAGMRVLGISCISNAAAGILD
QPLSHEEVMEMTEKVKGGFLKLVKAVVARSQK
>Mature_272_residues
MKQKIERAAAFIKEHVKETPKIGLILGSGLGVLADEIEGAVKLTYETIPDFPVSTVEGHAGQLVMGTLEGVQVIAMQGRF
HYYEGYSMDQVTFPVRVMKALGVESLVVTNAAGGINTEFRAGDLMIITDHINFTGTNPLIGPNEAEFGPRFPDMSEAYDK
ELSGLAEKTADELGIPVQKGVYTAVTGPSYETPAEVRFLRTMGSDAVGMSTVPEVIVANHAGMRVLGISCISNAAAGILD
QPLSHEEVMEMTEKVKGGFLKLVKAVVARSQK

Specific function: Cleavage of guanosine or inosine to respective bases and sugar-1-phosphate molecules [H]

COG id: COG0005

COG function: function code F; Purine nucleoside phosphorylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNP/MTAP phosphorylase family [H]

Homologues:

Organism=Homo sapiens, GI157168362, Length=275, Percent_Identity=48.7272727272727, Blast_Score=259, Evalue=1e-69,
Organism=Homo sapiens, GI47132622, Length=242, Percent_Identity=27.6859504132231, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI1788746, Length=245, Percent_Identity=47.7551020408163, Blast_Score=242, Evalue=2e-65,
Organism=Caenorhabditis elegans, GI17541190, Length=256, Percent_Identity=45.703125, Blast_Score=214, Evalue=3e-56,
Organism=Saccharomyces cerevisiae, GI6323238, Length=298, Percent_Identity=43.6241610738255, Blast_Score=220, Evalue=2e-58,
Organism=Drosophila melanogaster, GI45552887, Length=272, Percent_Identity=50.7352941176471, Blast_Score=264, Evalue=5e-71,
Organism=Drosophila melanogaster, GI45552885, Length=272, Percent_Identity=50.7352941176471, Blast_Score=263, Evalue=6e-71,
Organism=Drosophila melanogaster, GI24656093, Length=272, Percent_Identity=50.7352941176471, Blast_Score=263, Evalue=7e-71,
Organism=Drosophila melanogaster, GI24656090, Length=272, Percent_Identity=50.7352941176471, Blast_Score=263, Evalue=8e-71,
Organism=Drosophila melanogaster, GI24762376, Length=239, Percent_Identity=39.7489539748954, Blast_Score=159, Evalue=2e-39,
Organism=Drosophila melanogaster, GI20130079, Length=216, Percent_Identity=26.8518518518519, Blast_Score=68, Evalue=7e-12,
Organism=Drosophila melanogaster, GI221459247, Length=210, Percent_Identity=26.6666666666667, Blast_Score=65, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011268
- InterPro:   IPR000845
- InterPro:   IPR011270
- InterPro:   IPR001369
- InterPro:   IPR018099 [H]

Pfam domain/function: PF01048 PNP_UDP_1 [H]

EC number: =2.4.2.1 [H]

Molecular weight: Translated: 29143; Mature: 29143

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: PS00012 PHOSPHOPANTETHEINE ; PS01240 PNP_MTAP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQKIERAAAFIKEHVKETPKIGLILGSGLGVLADEIEGAVKLTYETIPDFPVSTVEGHA
CCHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHCCCEEEEEEECCCCCCCCCCCCCC
GQLVMGTLEGVQVIAMQGRFHYYEGYSMDQVTFPVRVMKALGVESLVVTNAAGGINTEFR
CEEEEEECCCEEEEEEECCEEEECCCCCCHHHHHHHHHHHCCHHHEEEECCCCCCCCCCC
AGDLMIITDHINFTGTNPLIGPNEAEFGPRFPDMSEAYDKELSGLAEKTADELGIPVQKG
CCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHCC
VYTAVTGPSYETPAEVRFLRTMGSDAVGMSTVPEVIVANHAGMRVLGISCISNAAAGILD
EEEEECCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHC
QPLSHEEVMEMTEKVKGGFLKLVKAVVARSQK
CCCCHHHHHHHHHHHCCHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKQKIERAAAFIKEHVKETPKIGLILGSGLGVLADEIEGAVKLTYETIPDFPVSTVEGHA
CCHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHCCCEEEEEEECCCCCCCCCCCCCC
GQLVMGTLEGVQVIAMQGRFHYYEGYSMDQVTFPVRVMKALGVESLVVTNAAGGINTEFR
CEEEEEECCCEEEEEEECCEEEECCCCCCHHHHHHHHHHHCCHHHEEEECCCCCCCCCCC
AGDLMIITDHINFTGTNPLIGPNEAEFGPRFPDMSEAYDKELSGLAEKTADELGIPVQKG
CCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHCC
VYTAVTGPSYETPAEVRFLRTMGSDAVGMSTVPEVIVANHAGMRVLGISCISNAAAGILD
EEEEECCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHC
QPLSHEEVMEMTEKVKGGFLKLVKAVVARSQK
CCCCHHHHHHHHHHHCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10537218; 8969508; 9384377; 1629150 [H]