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The map label for this gene is dacF [H]

Identifier: 154686590

GI number: 154686590

Start: 2248160

End: 2249329

Strand: Reverse

Name: dacF [H]

Synonym: RBAM_021590

Alternate gene names: 154686590

Gene position: 2249329-2248160 (Counterclockwise)

Preceding gene: 154686591

Following gene: 154686589

Centisome position: 57.4

GC content: 44.53

Gene sequence:

>1170_bases
ATGAAACGTCTTATATCCACTTTGTTGATTGGCATTTTATTAACGGCATCAGCACCGTCCGCTTTTGCAAAGCCGGACGG
AAAACATACATCGGAGCTCGCTCATGAAGCCAAAAGCGCCGTGCTGATTGAACGCGATACGGGAAGCATTCTTTATAACA
AGAACAGCCGGGAGCGTCTGGCGCCGGCAAGCATGACAAAAATCATGACCATGCTTTTAATCATGGAAGCGCTTGACAAA
GGGAAAATAAAGATGAGCGATAAAGTCAGGACGAGTGAACATGCCGCGTCCATGGGCGGATCACAGATTTTCCTTGAACC
CGGAGAAGAAATGACGGTAAAAGAAATGCTCAAAGGAATTGCGATAGCTTCCGGAAATGACGCATCTGTCGCAATGGCGG
AGTATATCGCCGGTTCAGAAGAGGATTTCGTCAGCCGGATGAACAAAAAAGCAAAAGAATTGGGATTGAAAGATACCTCC
TTCAAAAACCCGACGGGACTGCCTGAAAAAGACCATTACAGCTCTGCTTATGACATGGCGAAAATGGCGAAGGAATTATT
AAAGTATGATAAAATCACTCAATTTACGGGAACGTATGAGGATTATTTAAGAGAAAATACAGACAAAAAGTTCTGGCTCG
TCAATACAAACCGGCTGATCAAATTTTATCCGGGTGTAGACGGCGTGAAGACGGGCTTTACAGGCGAAGCGAAGTATTGT
TTAACCGCCACCGCTAAAAAAGGAAATATGAGGGTCATTGCTGTTGTTTTCGGAGCCAGCACCCCAAAAGAAAGAAATGC
CCAAGTGACCAAAATGCTTGATTACGCCTTCAGCCAGTTCAAAACACACCCGCTGTACAAACGGGATCAAATCGTCGGAA
CGGTAAAAGTAAAAAAAGGAAAACAGAAACTGATCAAGCTGACCACATCTGAGCCGATTTCTCTTCTGGCCAAAAAGGGA
GAGAACATGGACAAAGTAAAAAAAGAAGTGAAAATCAACGGCAATGTAACGGCTCCGGTTAAAAAAGGAGAAGTGCTCGG
CAGCCTCGTTCTGAAAAAAGACGGAAAGGTGCTCGTTGAAAGCCCAGTCACGGCAAAAGACGACATGGAAAAAGCGGGTT
TTCTCACTTTTCTGAAGCGGACGATGGGAGATTGGACTAAATTTAAATAA

Upstream 100 bases:

>100_bases
TTTTTTTGTGCCCTCAACACGAAACATTTTCCAGATGAGGCGTATAAAACCACCGGATTTGGAAAAAATAAAAAGGATTA
TGAAATGGAGGGCTTTGGAG

Downstream 100 bases:

>100_bases
TCGTGGCGAACGGGCACTAGTTTTGTCATAGTGAAGGAATTGCTTTCCCGAAAATCGAAACACTCATTATCCGATCATAT
CAAGGAGGAATGAGCATGAG

Product: DacF

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein dacF; PBP [H]

Number of amino acids: Translated: 389; Mature: 389

Protein sequence:

>389_residues
MKRLISTLLIGILLTASAPSAFAKPDGKHTSELAHEAKSAVLIERDTGSILYNKNSRERLAPASMTKIMTMLLIMEALDK
GKIKMSDKVRTSEHAASMGGSQIFLEPGEEMTVKEMLKGIAIASGNDASVAMAEYIAGSEEDFVSRMNKKAKELGLKDTS
FKNPTGLPEKDHYSSAYDMAKMAKELLKYDKITQFTGTYEDYLRENTDKKFWLVNTNRLIKFYPGVDGVKTGFTGEAKYC
LTATAKKGNMRVIAVVFGASTPKERNAQVTKMLDYAFSQFKTHPLYKRDQIVGTVKVKKGKQKLIKLTTSEPISLLAKKG
ENMDKVKKEVKINGNVTAPVKKGEVLGSLVLKKDGKVLVESPVTAKDDMEKAGFLTFLKRTMGDWTKFK

Sequences:

>Translated_389_residues
MKRLISTLLIGILLTASAPSAFAKPDGKHTSELAHEAKSAVLIERDTGSILYNKNSRERLAPASMTKIMTMLLIMEALDK
GKIKMSDKVRTSEHAASMGGSQIFLEPGEEMTVKEMLKGIAIASGNDASVAMAEYIAGSEEDFVSRMNKKAKELGLKDTS
FKNPTGLPEKDHYSSAYDMAKMAKELLKYDKITQFTGTYEDYLRENTDKKFWLVNTNRLIKFYPGVDGVKTGFTGEAKYC
LTATAKKGNMRVIAVVFGASTPKERNAQVTKMLDYAFSQFKTHPLYKRDQIVGTVKVKKGKQKLIKLTTSEPISLLAKKG
ENMDKVKKEVKINGNVTAPVKKGEVLGSLVLKKDGKVLVESPVTAKDDMEKAGFLTFLKRTMGDWTKFK
>Mature_389_residues
MKRLISTLLIGILLTASAPSAFAKPDGKHTSELAHEAKSAVLIERDTGSILYNKNSRERLAPASMTKIMTMLLIMEALDK
GKIKMSDKVRTSEHAASMGGSQIFLEPGEEMTVKEMLKGIAIASGNDASVAMAEYIAGSEEDFVSRMNKKAKELGLKDTS
FKNPTGLPEKDHYSSAYDMAKMAKELLKYDKITQFTGTYEDYLRENTDKKFWLVNTNRLIKFYPGVDGVKTGFTGEAKYC
LTATAKKGNMRVIAVVFGASTPKERNAQVTKMLDYAFSQFKTHPLYKRDQIVGTVKVKKGKQKLIKLTTSEPISLLAKKG
ENMDKVKKEVKINGNVTAPVKKGEVLGSLVLKKDGKVLVESPVTAKDDMEKAGFLTFLKRTMGDWTKFK

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Secreted (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1787062, Length=387, Percent_Identity=34.3669250645995, Blast_Score=207, Evalue=1e-54,
Organism=Escherichia coli, GI1786851, Length=365, Percent_Identity=31.7808219178082, Blast_Score=179, Evalue=3e-46,
Organism=Escherichia coli, GI87082022, Length=375, Percent_Identity=30.9333333333333, Blast_Score=173, Evalue=2e-44,
Organism=Escherichia coli, GI87082054, Length=225, Percent_Identity=29.3333333333333, Blast_Score=85, Evalue=9e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 43040; Mature: 43040

Theoretical pI: Translated: 10.27; Mature: 10.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRLISTLLIGILLTASAPSAFAKPDGKHTSELAHEAKSAVLIERDTGSILYNKNSRERL
CHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCEEEEEECCCCEEECCCCCHHC
APASMTKIMTMLLIMEALDKGKIKMSDKVRTSEHAASMGGSQIFLEPGEEMTVKEMLKGI
CHHHHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHE
AIASGNDASVAMAEYIAGSEEDFVSRMNKKAKELGLKDTSFKNPTGLPEKDHYSSAYDMA
EEECCCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHH
KMAKELLKYDKITQFTGTYEDYLRENTDKKFWLVNTNRLIKFYPGVDGVKTGFTGEAKYC
HHHHHHHHHHHHHHHCCCHHHHHHCCCCCEEEEEECCCEEEEECCCCCCCCCCCCCCCEE
LTATAKKGNMRVIAVVFGASTPKERNAQVTKMLDYAFSQFKTHPLYKRDQIVGTVKVKKG
EEEECCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCC
KQKLIKLTTSEPISLLAKKGENMDKVKKEVKINGNVTAPVKKGEVLGSLVLKKDGKVLVE
CCEEEEECCCCHHHHHHHCCCCHHHHHHHEEECCCEECCCCCCHHHHHHHEECCCCEEEE
SPVTAKDDMEKAGFLTFLKRTMGDWTKFK
CCCCCHHHHHHHHHHHHHHHHHCHHHCCC
>Mature Secondary Structure
MKRLISTLLIGILLTASAPSAFAKPDGKHTSELAHEAKSAVLIERDTGSILYNKNSRERL
CHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCEEEEEECCCCEEECCCCCHHC
APASMTKIMTMLLIMEALDKGKIKMSDKVRTSEHAASMGGSQIFLEPGEEMTVKEMLKGI
CHHHHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHE
AIASGNDASVAMAEYIAGSEEDFVSRMNKKAKELGLKDTSFKNPTGLPEKDHYSSAYDMA
EEECCCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHH
KMAKELLKYDKITQFTGTYEDYLRENTDKKFWLVNTNRLIKFYPGVDGVKTGFTGEAKYC
HHHHHHHHHHHHHHHCCCHHHHHHCCCCCEEEEEECCCEEEEECCCCCCCCCCCCCCCEE
LTATAKKGNMRVIAVVFGASTPKERNAQVTKMLDYAFSQFKTHPLYKRDQIVGTVKVKKG
EEEECCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCC
KQKLIKLTTSEPISLLAKKGENMDKVKKEVKINGNVTAPVKKGEVLGSLVLKKDGKVLVE
CCEEEEECCCCHHHHHHHCCCCHHHHHHHEEECCCEECCCCCCHHHHHHHEECCCCEEEE
SPVTAKDDMEKAGFLTFLKRTMGDWTKFK
CCCCCHHHHHHHHHHHHHHHHHCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1629150; 8969508; 9384377 [H]