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The map label for this gene is panB

Identifier: 154686490

GI number: 154686490

Start: 2159184

End: 2160017

Strand: Reverse

Name: panB

Synonym: RBAM_020580

Alternate gene names: 154686490

Gene position: 2160017-2159184 (Counterclockwise)

Preceding gene: 154686491

Following gene: 154686489

Centisome position: 55.12

GC content: 50.6

Gene sequence:

>834_bases
ATGAAAACAAAACTGGATTTCGTGAAAATGAAGGAAAACGGAGAGCCGATCGTAATGCTGACCGCTTACGATTATCCGCA
GGCAAAGCTTGCGGAGCAGGCGGGAGTCGACATGATTTTAGTCGGGGATTCGCTCGGAATGGTCGTGCTCGGACTCGATT
CAACAGTCGGCGTAACGGTCAGTGACATGATTCACCATACAAAAGCGGTAAAACGCGGTGCGAAAAATACGTTTATCGTA
ACTGACATGCCGTTTATGTCTTACCACCTTTCAAAAGAGGATACATTGAAAAACGCCGCGGCCATTATTCAGGAAAGCGG
CGCGGACGCGTTAAAGCTGGAAGGCGGAGACGGCGTTTTCGAATCCATCCGCGCGCTGACTCTCGGCGGAATTCCCGTCG
TCAGCCATTTAGGCCTCACACCGCAGTCTGTCGGCGTATTAGGCGGATATAAAGTACAGGGAAAAGATGAGCAAAGCGCG
AAAAAGCTCATTGAAGACAGCATCAAATGCGAGCAGGCGGGAGCGATGATGCTCGTGCTTGAATGCGTGCCTGCTGAACT
GACTGCTAAAATAAAAGAAGAAGTATCTATTCCCGTCATCGGAATCGGAGCCGGTTCAAAAGCGGACGGCCAAGTCCTTG
TCTATCATGACGTCGTCGGCCACGGCGTGGACAGAACCCCGAAGTTCGTGAAGCAATACGCCAAAATCGACGGCACGATT
GAGTCGGCCCTCAGCGGTTATGTCCGTGATGTGAAAGAACGGGTTTTCCCGGAAGAAAAGCATTCCTTTCAGATAAACCA
AACTGTGCTGCAAGGCTTGTACGGAGGAAAGTAA

Upstream 100 bases:

>100_bases
ACGTTAAACAAGTGGGAATCCTTCTTATTTTACAGAAGGCTTTTTTGCTTGCGAAGAATTGGCGGCTCATCTCCTCACCG
TTTAGAGGAGGAGAAACAGA

Downstream 100 bases:

>100_bases
GCGATGAAACAACTGACAGAAACTCAACAGCTGAAAGAATTGATTGCACAATACCGCTCAGAAGGCCGGACGATCGGATT
CGTCCCGACAATGGGTTTTC

Product: 3-methyl-2-oxobutanoate hydroxymethyltransferase

Products: NA

Alternate protein names: Ketopantoate hydroxymethyltransferase; KPHMT

Number of amino acids: Translated: 277; Mature: 277

Protein sequence:

>277_residues
MKTKLDFVKMKENGEPIVMLTAYDYPQAKLAEQAGVDMILVGDSLGMVVLGLDSTVGVTVSDMIHHTKAVKRGAKNTFIV
TDMPFMSYHLSKEDTLKNAAAIIQESGADALKLEGGDGVFESIRALTLGGIPVVSHLGLTPQSVGVLGGYKVQGKDEQSA
KKLIEDSIKCEQAGAMMLVLECVPAELTAKIKEEVSIPVIGIGAGSKADGQVLVYHDVVGHGVDRTPKFVKQYAKIDGTI
ESALSGYVRDVKERVFPEEKHSFQINQTVLQGLYGGK

Sequences:

>Translated_277_residues
MKTKLDFVKMKENGEPIVMLTAYDYPQAKLAEQAGVDMILVGDSLGMVVLGLDSTVGVTVSDMIHHTKAVKRGAKNTFIV
TDMPFMSYHLSKEDTLKNAAAIIQESGADALKLEGGDGVFESIRALTLGGIPVVSHLGLTPQSVGVLGGYKVQGKDEQSA
KKLIEDSIKCEQAGAMMLVLECVPAELTAKIKEEVSIPVIGIGAGSKADGQVLVYHDVVGHGVDRTPKFVKQYAKIDGTI
ESALSGYVRDVKERVFPEEKHSFQINQTVLQGLYGGK
>Mature_277_residues
MKTKLDFVKMKENGEPIVMLTAYDYPQAKLAEQAGVDMILVGDSLGMVVLGLDSTVGVTVSDMIHHTKAVKRGAKNTFIV
TDMPFMSYHLSKEDTLKNAAAIIQESGADALKLEGGDGVFESIRALTLGGIPVVSHLGLTPQSVGVLGGYKVQGKDEQSA
KKLIEDSIKCEQAGAMMLVLECVPAELTAKIKEEVSIPVIGIGAGSKADGQVLVYHDVVGHGVDRTPKFVKQYAKIDGTI
ESALSGYVRDVKERVFPEEKHSFQINQTVLQGLYGGK

Specific function: Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is tranferred onto alpha-ketoisovalerate to form ketopantoate

COG id: COG0413

COG function: function code H; Ketopantoate hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the panB family

Homologues:

Organism=Escherichia coli, GI1786326, Length=256, Percent_Identity=47.265625, Blast_Score=246, Evalue=1e-66,
Organism=Saccharomyces cerevisiae, GI6319653, Length=262, Percent_Identity=38.5496183206107, Blast_Score=187, Evalue=2e-48,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PANB_BACA2 (A7Z5Z4)

Other databases:

- EMBL:   CP000560
- RefSeq:   YP_001421651.1
- ProteinModelPortal:   A7Z5Z4
- SMR:   A7Z5Z4
- STRING:   A7Z5Z4
- EnsemblBacteria:   EBBACT00000005509
- GeneID:   5461377
- GenomeReviews:   CP000560_GR
- KEGG:   bay:RBAM_020580
- eggNOG:   COG0413
- GeneTree:   EBGT00050000000505
- HOGENOM:   HBG299908
- OMA:   YATPEQT
- ProtClustDB:   PRK00311
- BioCyc:   BAMY326423:RBAM_020580-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00156
- InterPro:   IPR003700
- InterPro:   IPR015813
- Gene3D:   G3DSA:3.20.20.60
- PANTHER:   PTHR20881
- PIRSF:   PIRSF000388
- TIGRFAMs:   TIGR00222

Pfam domain/function: PF02548 Pantoate_transf; SSF51621 Pyrv/PenolPyrv_Kinase_cat

EC number: =2.1.2.11

Molecular weight: Translated: 29692; Mature: 29692

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: NA

Important sites: ACT_SITE 181-181 BINDING 82-82 BINDING 112-112

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTKLDFVKMKENGEPIVMLTAYDYPQAKLAEQAGVDMILVGDSLGMVVLGLDSTVGVTV
CCCCCCEEEEECCCCCEEEEEECCCCHHHHHHHCCCCEEEECCCCCEEEEECCCCCCCHH
SDMIHHTKAVKRGAKNTFIVTDMPFMSYHLSKEDTLKNAAAIIQESGADALKLEGGDGVF
HHHHHHHHHHHCCCCCEEEEECCCHHHEECCCHHHHHHHHHHHHCCCCCEEEEECCCCHH
ESIRALTLGGIPVVSHLGLTPQSVGVLGGYKVQGKDEQSAKKLIEDSIKCEQAGAMMLVL
HHHHHHHCCCCHHHHHCCCCHHHCCEECCEEECCCCHHHHHHHHHHHHHHHHCCCEEEEE
ECVPAELTAKIKEEVSIPVIGIGAGSKADGQVLVYHDVVGHGVDRTPKFVKQYAKIDGTI
ECCCHHHHHHHHHCCCCCEEEECCCCCCCCEEEEEEEHHCCCCCCCHHHHHHHHHHCCHH
ESALSGYVRDVKERVFPEEKHSFQINQTVLQGLYGGK
HHHHHHHHHHHHHHCCCCCHHCEEHHHHHHHHHCCCC
>Mature Secondary Structure
MKTKLDFVKMKENGEPIVMLTAYDYPQAKLAEQAGVDMILVGDSLGMVVLGLDSTVGVTV
CCCCCCEEEEECCCCCEEEEEECCCCHHHHHHHCCCCEEEECCCCCEEEEECCCCCCCHH
SDMIHHTKAVKRGAKNTFIVTDMPFMSYHLSKEDTLKNAAAIIQESGADALKLEGGDGVF
HHHHHHHHHHHCCCCCEEEEECCCHHHEECCCHHHHHHHHHHHHCCCCCEEEEECCCCHH
ESIRALTLGGIPVVSHLGLTPQSVGVLGGYKVQGKDEQSAKKLIEDSIKCEQAGAMMLVL
HHHHHHHCCCCHHHHHCCCCHHHCCEECCEEECCCCHHHHHHHHHHHHHHHHCCCEEEEE
ECVPAELTAKIKEEVSIPVIGIGAGSKADGQVLVYHDVVGHGVDRTPKFVKQYAKIDGTI
ECCCHHHHHHHHHCCCCCEEEECCCCCCCCEEEEEEEHHCCCCCCCHHHHHHHHHHCCHH
ESALSGYVRDVKERVFPEEKHSFQINQTVLQGLYGGK
HHHHHHHHHHHHHHCCCCCHHCEEHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA