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The map label for this gene is yngG [H]

Identifier: 154686268

GI number: 154686268

Start: 1923752

End: 1924651

Strand: Reverse

Name: yngG [H]

Synonym: RBAM_018350

Alternate gene names: 154686268

Gene position: 1924651-1923752 (Counterclockwise)

Preceding gene: 154686269

Following gene: 154686267

Centisome position: 49.12

GC content: 50.56

Gene sequence:

>900_bases
ATGAACTGTCCGAAACATGTCCGCATCAATGAAGTAGGACCGCGTGACGGCTTGCAGAATGAATCGGCGTGGGTGGATAC
AGAAGATAAGATTGAGTGGATCAATATGCTGTCAAAAACAGGCCTTCCCTATATCGAAGTGACATCATTCGTGCATCCGA
GATGGATTCCGGCTCTTCGTGACAGCCTGGACGTCGCAAAAGGCATTGCCAGATCTGAACATACGGTCTACGCGGCTCTC
GTTCCGAACCTCATCGGACTGGAGCATGCGGCAGAAGGCGGAATCGATCAGGCATGTGTATTTTTATCTGCAAGCGAAAC
CCATAACCAAAAAAACGTAAACAAACCGATTGACCGGACCGTTCAAGAGCTCAGGCGGGTTATTACTGAAGCAAAAGCTG
AAAAAATGGCGACGAGAGCTTATCTGTCCACTGTGTTCGGCTGTCCGTACGAACAGAACGTGCCGATCGAACAAGTCATC
CGCCTGTCAGACACCCTGCTCGAAATGGGTGTTGATGAAATATCGCTCGGCGACACAATCGGCGCGGCAAACCCCTTGCA
GGTCATAGCCGTGCTTGAAGCCCTTTTGCCGAGAATTCCCGCCATAAAAATCGCACTCCATTTCCATGATACAAGAGGAA
CGGCTCTAGCGAATATCATTCCCGCTCTTGATATGGGCATCACATCCTTTGATACTTCCTCAGGGGGGCTTGGCGGCTGC
CCGTACGCCCCTGGATCAGCAGGAAACGCTGCCACAGAAGACGTCATTTATATGCTCGAACAAATGGGGATTGATACCGG
GACGGACATGAATAAACTTCTTTATGCCGCCGGCTGGATCGCGGATAAAATAGGCAAAGACCTGCCAAGCAGAAACCTGC
AGGCATTCAGAACGAAATGA

Upstream 100 bases:

>100_bases
TGATGCAGCGGGTACGGTGAAAGAAGTGAAAAAATCAGAAGGTGATTTCACTGATGAAGGAGAGGTCCTGATTGAACTTC
AATAAAACGGGAGGTGCAGC

Downstream 100 bases:

>100_bases
GAAAGGAGAAGCCATGGGAGATTGCGTTTTGTATTCCGTCTGTCAAGAAACAATCGGTCTGATCACGCTGAACCGCCCGC
ATGCGGCCAACTCGCTGTCT

Product: hydroxymethylglutaryl-CoA lyase

Products: NA

Alternate protein names: HL; HMG-CoA lyase; 3-hydroxy-3-methylglutarate-CoA lyase [H]

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MNCPKHVRINEVGPRDGLQNESAWVDTEDKIEWINMLSKTGLPYIEVTSFVHPRWIPALRDSLDVAKGIARSEHTVYAAL
VPNLIGLEHAAEGGIDQACVFLSASETHNQKNVNKPIDRTVQELRRVITEAKAEKMATRAYLSTVFGCPYEQNVPIEQVI
RLSDTLLEMGVDEISLGDTIGAANPLQVIAVLEALLPRIPAIKIALHFHDTRGTALANIIPALDMGITSFDTSSGGLGGC
PYAPGSAGNAATEDVIYMLEQMGIDTGTDMNKLLYAAGWIADKIGKDLPSRNLQAFRTK

Sequences:

>Translated_299_residues
MNCPKHVRINEVGPRDGLQNESAWVDTEDKIEWINMLSKTGLPYIEVTSFVHPRWIPALRDSLDVAKGIARSEHTVYAAL
VPNLIGLEHAAEGGIDQACVFLSASETHNQKNVNKPIDRTVQELRRVITEAKAEKMATRAYLSTVFGCPYEQNVPIEQVI
RLSDTLLEMGVDEISLGDTIGAANPLQVIAVLEALLPRIPAIKIALHFHDTRGTALANIIPALDMGITSFDTSSGGLGGC
PYAPGSAGNAATEDVIYMLEQMGIDTGTDMNKLLYAAGWIADKIGKDLPSRNLQAFRTK
>Mature_299_residues
MNCPKHVRINEVGPRDGLQNESAWVDTEDKIEWINMLSKTGLPYIEVTSFVHPRWIPALRDSLDVAKGIARSEHTVYAAL
VPNLIGLEHAAEGGIDQACVFLSASETHNQKNVNKPIDRTVQELRRVITEAKAEKMATRAYLSTVFGCPYEQNVPIEQVI
RLSDTLLEMGVDEISLGDTIGAANPLQVIAVLEALLPRIPAIKIALHFHDTRGTALANIIPALDMGITSFDTSSGGLGGC
PYAPGSAGNAATEDVIYMLEQMGIDTGTDMNKLLYAAGWIADKIGKDLPSRNLQAFRTK

Specific function: Involved in the catabolism of branched amino acids such as leucine (Probable) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HMG-CoA lyase family [H]

Homologues:

Organism=Homo sapiens, GI109150422, Length=292, Percent_Identity=45.8904109589041, Blast_Score=269, Evalue=3e-72,
Organism=Homo sapiens, GI109150427, Length=292, Percent_Identity=45.8904109589041, Blast_Score=268, Evalue=3e-72,
Organism=Homo sapiens, GI62198232, Length=292, Percent_Identity=44.1780821917808, Blast_Score=263, Evalue=1e-70,
Organism=Homo sapiens, GI260654708, Length=292, Percent_Identity=37.6712328767123, Blast_Score=177, Evalue=1e-44,
Organism=Caenorhabditis elegans, GI17510563, Length=285, Percent_Identity=44.2105263157895, Blast_Score=246, Evalue=1e-65,
Organism=Saccharomyces cerevisiae, GI6320019, Length=224, Percent_Identity=26.7857142857143, Blast_Score=73, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6320071, Length=224, Percent_Identity=26.7857142857143, Blast_Score=72, Evalue=8e-14,
Organism=Drosophila melanogaster, GI24582381, Length=292, Percent_Identity=44.8630136986301, Blast_Score=260, Evalue=8e-70,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like [H]

EC number: =4.1.3.4 [H]

Molecular weight: Translated: 32517; Mature: 32517

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNCPKHVRINEVGPRDGLQNESAWVDTEDKIEWINMLSKTGLPYIEVTSFVHPRWIPALR
CCCCCCCEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEHHHHCCCCHHHHHH
DSLDVAKGIARSEHTVYAALVPNLIGLEHAAEGGIDQACVFLSASETHNQKNVNKPIDRT
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEECHHCCCCCCCCCHHHHH
VQELRRVITEAKAEKMATRAYLSTVFGCPYEQNVPIEQVIRLSDTLLEMGVDEISLGDTI
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCHHCCCCCC
GAANPLQVIAVLEALLPRIPAIKIALHFHDTRGTALANIIPALDMGITSFDTSSGGLGGC
CCCCHHHHHHHHHHHHCCCCEEEEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCCC
PYAPGSAGNAATEDVIYMLEQMGIDTGTDMNKLLYAAGWIADKIGKDLPSRNLQAFRTK
CCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHCCC
>Mature Secondary Structure
MNCPKHVRINEVGPRDGLQNESAWVDTEDKIEWINMLSKTGLPYIEVTSFVHPRWIPALR
CCCCCCCEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEHHHHCCCCHHHHHH
DSLDVAKGIARSEHTVYAALVPNLIGLEHAAEGGIDQACVFLSASETHNQKNVNKPIDRT
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEECHHCCCCCCCCCHHHHH
VQELRRVITEAKAEKMATRAYLSTVFGCPYEQNVPIEQVIRLSDTLLEMGVDEISLGDTI
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCHHCCCCCC
GAANPLQVIAVLEALLPRIPAIKIALHFHDTRGTALANIIPALDMGITSFDTSSGGLGGC
CCCCHHHHHHHHHHHHCCCCEEEEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCCC
PYAPGSAGNAATEDVIYMLEQMGIDTGTDMNKLLYAAGWIADKIGKDLPSRNLQAFRTK
CCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9387222; 9384377 [H]