BacMap

Definition	Bacillus amyloliquefaciens FZB42, complete genome.
Accession	NC_009725
Length	3,918,589

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The map label for this gene is yngF [H]

Identifier: 154686267

GI number: 154686267

Start: 1922959

End: 1923738

Strand: Reverse

Name: yngF [H]

Synonym: RBAM_018340

Alternate gene names: 154686267

Gene position: 1923738-1922959 (Counterclockwise)

Preceding gene: 154686268

Following gene: 154686266

Centisome position: 49.09

GC content: 52.31

Gene sequence:

>780_bases
ATGGGAGATTGCGTTTTGTATTCCGTCTGTCAAGAAACAATCGGTCTGATCACGCTGAACCGCCCGCATGCGGCCAACTC
GCTGTCTTCAGAAATGATTGATCATTTACAGCGTGTGATTGAAGACATCAAAAACTCGCCATCCATTCGTTGTGTCATTC
TCACCGGCACCGGGAACACATTTTGCGCCGGTGCTGATTTAAAAGAAAGAGCGGGGATGAATCATGAACAAGTTAAGCAA
AGCGTCAGGCGTATCGGTGAAACGGCCGCATCCATTGAAGCTCTCCCGCAGCCGGTCATTGCAGCGCTGAACGGAACCGC
TGTCGGAGGAGGCCTTGAACTCGCTCTCGCCTGCGATATCAGAACAGCCGTCAGCCGGGCGGGCCTCGGCCTTCCTGAAA
CAGGGCTTGCGATCATTCCCGGAGCGGGCGGAACGCAGAGACTGCCCCGGCTTATCGGCCCCGGCGCGGCAAAAGAATTG
ATCTATACCGGCCGAAGACTTTCTGCAGAAGAGGGAAAAGACATGAAGCTGATCGAGCATGTTTGTGAAGCCGATGAACT
TATGGAAAAAGCAAAAGCACTGGCCGGCCGCATCGCCGCTAATGGGCCGATCGCCGTCAGACAAGCAAAATCCGCGATAA
ACAAGGGTCTGGAGACAGATCTCAATACAGGGCTCGAAATCGAACGAAAAGCATATGAACAAGTCATTCCCACACTTGAC
AGAGAAGAGGGCCTGCGCGCTTTTAAAGAAAAAAGACCCCCTGAATACAAAGGAAAATAA

Upstream 100 bases:

>100_bases
AAACTTCTTTATGCCGCCGGCTGGATCGCGGATAAAATAGGCAAAGACCTGCCAAGCAGAAACCTGCAGGCATTCAGAAC
GAAATGAGAAAGGAGAAGCC

Downstream 100 bases:

>100_bases
AACATGGAGGGACTCGGATGGATTACGAAAAAGAACGGCAGACACGCAGCAAAACGATTGAAAAGGGAGGAGCCGCGAAA
TATCACGCAAGCAACGAAAA

Product: enoyl-CoA hydratase

Products: trans-2-enoyl-CoA; trans-3-enoyl-CoA; H2O

Alternate protein names: NA

Number of amino acids: Translated: 259; Mature: 258

Protein sequence:

>259_residues
MGDCVLYSVCQETIGLITLNRPHAANSLSSEMIDHLQRVIEDIKNSPSIRCVILTGTGNTFCAGADLKERAGMNHEQVKQ
SVRRIGETAASIEALPQPVIAALNGTAVGGGLELALACDIRTAVSRAGLGLPETGLAIIPGAGGTQRLPRLIGPGAAKEL
IYTGRRLSAEEGKDMKLIEHVCEADELMEKAKALAGRIAANGPIAVRQAKSAINKGLETDLNTGLEIERKAYEQVIPTLD
REEGLRAFKEKRPPEYKGK

Sequences:

>Translated_259_residues
MGDCVLYSVCQETIGLITLNRPHAANSLSSEMIDHLQRVIEDIKNSPSIRCVILTGTGNTFCAGADLKERAGMNHEQVKQ
SVRRIGETAASIEALPQPVIAALNGTAVGGGLELALACDIRTAVSRAGLGLPETGLAIIPGAGGTQRLPRLIGPGAAKEL
IYTGRRLSAEEGKDMKLIEHVCEADELMEKAKALAGRIAANGPIAVRQAKSAINKGLETDLNTGLEIERKAYEQVIPTLD
REEGLRAFKEKRPPEYKGK
>Mature_258_residues
GDCVLYSVCQETIGLITLNRPHAANSLSSEMIDHLQRVIEDIKNSPSIRCVILTGTGNTFCAGADLKERAGMNHEQVKQS
VRRIGETAASIEALPQPVIAALNGTAVGGGLELALACDIRTAVSRAGLGLPETGLAIIPGAGGTQRLPRLIGPGAAKELI
YTGRRLSAEEGKDMKLIEHVCEADELMEKAKALAGRIAANGPIAVRQAKSAINKGLETDLNTGLEIERKAYEQVIPTLDR
EEGLRAFKEKRPPEYKGK

Specific function: Could Possibly Oxidizes Fatty Acids Using Specific Components (By Similarity). [C]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI4502327, Length=256, Percent_Identity=51.5625, Blast_Score=208, Evalue=3e-54,
Organism=Homo sapiens, GI194097323, Length=245, Percent_Identity=39.5918367346939, Blast_Score=187, Evalue=1e-47,
Organism=Homo sapiens, GI150378541, Length=248, Percent_Identity=39.1129032258064, Blast_Score=157, Evalue=7e-39,
Organism=Homo sapiens, GI20127408, Length=254, Percent_Identity=32.6771653543307, Blast_Score=112, Evalue=2e-25,
Organism=Homo sapiens, GI68989263, Length=193, Percent_Identity=33.6787564766839, Blast_Score=106, Evalue=2e-23,
Organism=Homo sapiens, GI31542718, Length=247, Percent_Identity=29.5546558704453, Blast_Score=101, Evalue=5e-22,
Organism=Homo sapiens, GI70995211, Length=213, Percent_Identity=31.924882629108, Blast_Score=100, Evalue=1e-21,
Organism=Homo sapiens, GI157694516, Length=220, Percent_Identity=27.7272727272727, Blast_Score=88, Evalue=9e-18,
Organism=Homo sapiens, GI213417737, Length=220, Percent_Identity=27.7272727272727, Blast_Score=88, Evalue=9e-18,
Organism=Homo sapiens, GI261878539, Length=102, Percent_Identity=45.0980392156863, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI45643119, Length=215, Percent_Identity=26.5116279069767, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI260275230, Length=215, Percent_Identity=26.5116279069767, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI37594471, Length=179, Percent_Identity=29.0502793296089, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI260274832, Length=215, Percent_Identity=26.5116279069767, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI37594469, Length=179, Percent_Identity=29.0502793296089, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI221136756, Length=238, Percent_Identity=21.0084033613445, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI221136753, Length=238, Percent_Identity=21.0084033613445, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI157694520, Length=202, Percent_Identity=27.7227722772277, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI116235487, Length=200, Percent_Identity=22.5, Blast_Score=75, Evalue=9e-14,
Organism=Homo sapiens, GI221307494, Length=210, Percent_Identity=23.3333333333333, Blast_Score=75, Evalue=9e-14,
Organism=Homo sapiens, GI25453481, Length=245, Percent_Identity=20, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI51538573, Length=245, Percent_Identity=20, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI4757966, Length=217, Percent_Identity=21.1981566820277, Blast_Score=68, Evalue=9e-12,
Organism=Homo sapiens, GI51538581, Length=217, Percent_Identity=21.1981566820277, Blast_Score=68, Evalue=9e-12,
Organism=Homo sapiens, GI4757968, Length=246, Percent_Identity=19.9186991869919, Blast_Score=66, Evalue=4e-11,
Organism=Homo sapiens, GI49355787, Length=246, Percent_Identity=19.9186991869919, Blast_Score=66, Evalue=4e-11,
Organism=Escherichia coli, GI1787659, Length=249, Percent_Identity=38.1526104417671, Blast_Score=174, Evalue=7e-45,
Organism=Escherichia coli, GI221142681, Length=249, Percent_Identity=35.7429718875502, Blast_Score=137, Evalue=9e-34,
Organism=Escherichia coli, GI1787660, Length=266, Percent_Identity=34.5864661654135, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI1788682, Length=214, Percent_Identity=35.981308411215, Blast_Score=117, Evalue=5e-28,
Organism=Escherichia coli, GI1790281, Length=215, Percent_Identity=33.4883720930233, Blast_Score=115, Evalue=2e-27,
Organism=Escherichia coli, GI1788597, Length=256, Percent_Identity=27.34375, Blast_Score=107, Evalue=9e-25,
Organism=Escherichia coli, GI87082183, Length=260, Percent_Identity=27.3076923076923, Blast_Score=104, Evalue=7e-24,
Organism=Caenorhabditis elegans, GI17540714, Length=225, Percent_Identity=45.7777777777778, Blast_Score=203, Evalue=7e-53,
Organism=Caenorhabditis elegans, GI25145438, Length=245, Percent_Identity=40.4081632653061, Blast_Score=187, Evalue=3e-48,
Organism=Caenorhabditis elegans, GI17554946, Length=249, Percent_Identity=37.7510040160643, Blast_Score=180, Evalue=6e-46,
Organism=Caenorhabditis elegans, GI17534483, Length=210, Percent_Identity=30, Blast_Score=106, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17560910, Length=246, Percent_Identity=30.0813008130081, Blast_Score=105, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17536985, Length=257, Percent_Identity=24.5136186770428, Blast_Score=104, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI17558304, Length=235, Percent_Identity=27.6595744680851, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17508953, Length=235, Percent_Identity=28.5106382978723, Blast_Score=95, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17508951, Length=235, Percent_Identity=28.5106382978723, Blast_Score=95, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI25144276, Length=235, Percent_Identity=28.5106382978723, Blast_Score=94, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI17549921, Length=201, Percent_Identity=26.3681592039801, Blast_Score=81, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI25141363, Length=202, Percent_Identity=25.7425742574257, Blast_Score=80, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI25144160, Length=188, Percent_Identity=30.3191489361702, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI25144157, Length=188, Percent_Identity=30.3191489361702, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17535521, Length=208, Percent_Identity=27.4038461538462, Blast_Score=75, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17540306, Length=245, Percent_Identity=26.530612244898, Blast_Score=71, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6320241, Length=224, Percent_Identity=25.8928571428571, Blast_Score=84, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24653139, Length=255, Percent_Identity=52.5490196078431, Blast_Score=249, Evalue=1e-66,
Organism=Drosophila melanogaster, GI20129971, Length=245, Percent_Identity=38.7755102040816, Blast_Score=183, Evalue=8e-47,
Organism=Drosophila melanogaster, GI24653477, Length=245, Percent_Identity=38.7755102040816, Blast_Score=183, Evalue=8e-47,
Organism=Drosophila melanogaster, GI19920382, Length=227, Percent_Identity=28.1938325991189, Blast_Score=105, Evalue=3e-23,
Organism=Drosophila melanogaster, GI19922422, Length=242, Percent_Identity=26.4462809917355, Blast_Score=97, Evalue=8e-21,
Organism=Drosophila melanogaster, GI24650670, Length=242, Percent_Identity=28.9256198347107, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI21357171, Length=172, Percent_Identity=30.2325581395349, Blast_Score=88, Evalue=6e-18,
Organism=Drosophila melanogaster, GI28571729, Length=183, Percent_Identity=33.879781420765, Blast_Score=88, Evalue=7e-18,
Organism=Drosophila melanogaster, GI28571730, Length=183, Percent_Identity=33.879781420765, Blast_Score=88, Evalue=7e-18,
Organism=Drosophila melanogaster, GI19921000, Length=249, Percent_Identity=29.718875502008, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24583077, Length=239, Percent_Identity=30.5439330543933, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24583079, Length=239, Percent_Identity=30.5439330543933, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI19921018, Length=193, Percent_Identity=27.4611398963731, Blast_Score=77, Evalue=9e-15,
Organism=Drosophila melanogaster, GI24654903, Length=171, Percent_Identity=25.1461988304094, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24583165, Length=194, Percent_Identity=28.8659793814433, Blast_Score=77, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: 4.2.1.17

Molecular weight: Translated: 27613; Mature: 27482

Theoretical pI: Translated: 7.37; Mature: 7.37

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGDCVLYSVCQETIGLITLNRPHAANSLSSEMIDHLQRVIEDIKNSPSIRCVILTGTGNT
CCCHHHHHHHHHHHCEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCC
FCAGADLKERAGMNHEQVKQSVRRIGETAASIEALPQPVIAALNGTAVGGGLELALACDI
EEECCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCEECCCEEEEEEHHH
RTAVSRAGLGLPETGLAIIPGAGGTQRLPRLIGPGAAKELIYTGRRLSAEEGKDMKLIEH
HHHHHHCCCCCCCCCEEEECCCCCHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCHHHHHH
VCEADELMEKAKALAGRIAANGPIAVRQAKSAINKGLETDLNTGLEIERKAYEQVIPTLD
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCC
REEGLRAFKEKRPPEYKGK
HHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
GDCVLYSVCQETIGLITLNRPHAANSLSSEMIDHLQRVIEDIKNSPSIRCVILTGTGNT
CCHHHHHHHHHHHCEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCC
FCAGADLKERAGMNHEQVKQSVRRIGETAASIEALPQPVIAALNGTAVGGGLELALACDI
EEECCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCEECCCEEEEEEHHH
RTAVSRAGLGLPETGLAIIPGAGGTQRLPRLIGPGAAKELIYTGRRLSAEEGKDMKLIEH
HHHHHHCCCCCCCCCEEEECCCCCHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCHHHHHH
VCEADELMEKAKALAGRIAANGPIAVRQAKSAINKGLETDLNTGLEIERKAYEQVIPTLD
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCC
REEGLRAFKEKRPPEYKGK
HHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.05 {crotonyl-CoA}} 0.008 {2-decenoyl-CoA}} [C]

Substrates: (3S)-3-hydroxyacyl-CoA

Specific reaction: (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O

General reaction: addition (of H2O to carbon carbon double bondelimination (of H2O C-O bond cleavagehydration [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9387222; 9384377 [H]