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The map label for this gene is bioD

Identifier: 154686259

GI number: 154686259

Start: 1915147

End: 1915848

Strand: Reverse

Name: bioD

Synonym: RBAM_018260

Alternate gene names: 154686259

Gene position: 1915848-1915147 (Counterclockwise)

Preceding gene: 154686260

Following gene: 154686258

Centisome position: 48.89

GC content: 47.58

Gene sequence:

>702_bases
ATGAGAAGTTTTTTTGTTACAGGAACAGATACCGGCGTCGGAAAAACCATCGTTTCTTGCGGACTTGCCGCGGCATTCCG
GCAGAAACAGGCGGATATCGGAGTGTTTAAGCCGTTTTTAAGCGGCGTGCCTCGGACTCATCCGGAAAGTGACACCTCGC
TCTTGAAAGACATGTCACAAACCGCACTGTCTCATGAAGAAATCACGCCATTTGAATGGAAAGAGCCGCTTGCCCCGTAC
ACGGCCGCCATGCTTGAGGGGAAATCCGTGAGCATGGCGGATGTCATGGCCCACTGGAGTAAGATCAAAAATCGTCATGA
GTGTTTCATCGTTGAGGGGGCAGGCGGCATTTCCGTCCCGCTTGGTGAAAATTATTTAGTCAGTGATCTGATAAAAGCCA
TGGAATTACCGGCAGTGATTGTCACCAGGCCTGATCTCGGAACCATCAACCACACATATTTAACCGTTGAATATGCGAAA
AACATGGGGATAGAGGTTCTCGGCATTATTATCAACGGCGTAAGGAGCAGGCCGGGGCTGGACGAAAAGACAAACCCGAG
CATGATTGAAACATTATGCAAAGTTCCGATTTTGGGAATCACACCGAAGCTGGATCACGTTTCATCGGAAAACATTCAAC
ACATGATTGAGAAACATATAGATGTTTCCTCTTTCATGAATCTGACACAAATGGGGAGATAA

Upstream 100 bases:

>100_bases
GCAGAATCAGGCTGACCGTTACGGCGGATCGCAGCTTACAGGATATAGACGAGCTGACAGAAGCATTTGATTCCATTAGA
AAAGAGCTGAATATAAACAA

Downstream 100 bases:

>100_bases
ACATGAATCAATGGATGGATCTTGCAGAGAGAGTGCTGGACGGGGCGGAAGTAACGAATCAAGAAGCGCTGGCCATTTTA
AATTGTCCTGATGAGGATCT

Product: dithiobiotin synthetase

Products: NA

Alternate protein names: DTB synthetase; DTBS; Dethiobiotin synthase

Number of amino acids: Translated: 233; Mature: 233

Protein sequence:

>233_residues
MRSFFVTGTDTGVGKTIVSCGLAAAFRQKQADIGVFKPFLSGVPRTHPESDTSLLKDMSQTALSHEEITPFEWKEPLAPY
TAAMLEGKSVSMADVMAHWSKIKNRHECFIVEGAGGISVPLGENYLVSDLIKAMELPAVIVTRPDLGTINHTYLTVEYAK
NMGIEVLGIIINGVRSRPGLDEKTNPSMIETLCKVPILGITPKLDHVSSENIQHMIEKHIDVSSFMNLTQMGR

Sequences:

>Translated_233_residues
MRSFFVTGTDTGVGKTIVSCGLAAAFRQKQADIGVFKPFLSGVPRTHPESDTSLLKDMSQTALSHEEITPFEWKEPLAPY
TAAMLEGKSVSMADVMAHWSKIKNRHECFIVEGAGGISVPLGENYLVSDLIKAMELPAVIVTRPDLGTINHTYLTVEYAK
NMGIEVLGIIINGVRSRPGLDEKTNPSMIETLCKVPILGITPKLDHVSSENIQHMIEKHIDVSSFMNLTQMGR
>Mature_233_residues
MRSFFVTGTDTGVGKTIVSCGLAAAFRQKQADIGVFKPFLSGVPRTHPESDTSLLKDMSQTALSHEEITPFEWKEPLAPY
TAAMLEGKSVSMADVMAHWSKIKNRHECFIVEGAGGISVPLGENYLVSDLIKAMELPAVIVTRPDLGTINHTYLTVEYAK
NMGIEVLGIIINGVRSRPGLDEKTNPSMIETLCKVPILGITPKLDHVSSENIQHMIEKHIDVSSFMNLTQMGR

Specific function: Bioconversion of pimelate into dethiobiotin. [C]

COG id: COG0132

COG function: function code H; Dethiobiotin synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dethiobiotin synthetase family

Homologues:

Organism=Escherichia coli, GI1786995, Length=207, Percent_Identity=30.9178743961353, Blast_Score=107, Evalue=9e-25,
Organism=Escherichia coli, GI87081944, Length=224, Percent_Identity=26.3392857142857, Blast_Score=74, Evalue=7e-15,
Organism=Saccharomyces cerevisiae, GI6324385, Length=184, Percent_Identity=33.1521739130435, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOD_BACA2 (A7Z5B3)

Other databases:

- EMBL:   CP000560
- RefSeq:   YP_001421420.1
- ProteinModelPortal:   A7Z5B3
- SMR:   A7Z5B3
- STRING:   A7Z5B3
- EnsemblBacteria:   EBBACT00000006893
- GeneID:   5459522
- GenomeReviews:   CP000560_GR
- KEGG:   bay:RBAM_018260
- eggNOG:   COG0132
- GeneTree:   EBGT00050000001962
- HOGENOM:   HBG650065
- OMA:   PQMERIE
- ProtClustDB:   PRK00090
- BioCyc:   BAMY326423:RBAM_018260-MONOMER
- HAMAP:   MF_00336
- InterPro:   IPR004472
- InterPro:   IPR002586
- PIRSF:   PIRSF006755
- TIGRFAMs:   TIGR00347

Pfam domain/function: PF01656 CbiA

EC number: =6.3.3.3

Molecular weight: Translated: 25540; Mature: 25540

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSFFVTGTDTGVGKTIVSCGLAAAFRQKQADIGVFKPFLSGVPRTHPESDTSLLKDMSQ
CCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCHHHHHHHHHH
TALSHEEITPFEWKEPLAPYTAAMLEGKSVSMADVMAHWSKIKNRHECFIVEGAGGISVP
HHHHHCCCCCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCEECC
LGENYLVSDLIKAMELPAVIVTRPDLGTINHTYLTVEYAKNMGIEVLGIIINGVRSRPGL
CCCCHHHHHHHHHHCCCEEEEECCCCCCCCEEEEEEEHHHCCCCEEEHHHHHHHCCCCCC
DEKTNPSMIETLCKVPILGITPKLDHVSSENIQHMIEKHIDVSSFMNLTQMGR
CCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHCCC
>Mature Secondary Structure
MRSFFVTGTDTGVGKTIVSCGLAAAFRQKQADIGVFKPFLSGVPRTHPESDTSLLKDMSQ
CCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCHHHHHHHHHH
TALSHEEITPFEWKEPLAPYTAAMLEGKSVSMADVMAHWSKIKNRHECFIVEGAGGISVP
HHHHHCCCCCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCEECC
LGENYLVSDLIKAMELPAVIVTRPDLGTINHTYLTVEYAKNMGIEVLGIIINGVRSRPGL
CCCCHHHHHHHHHHCCCEEEEECCCCCCCCEEEEEEEHHHCCCCEEEHHHHHHHCCCCCC
DEKTNPSMIETLCKVPILGITPKLDHVSSENIQHMIEKHIDVSSFMNLTQMGR
CCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA