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The map label for this gene is bioF

Identifier: 154686260

GI number: 154686260

Start: 1915845

End: 1917005

Strand: Reverse

Name: bioF

Synonym: RBAM_018270

Alternate gene names: 154686260

Gene position: 1917005-1915845 (Counterclockwise)

Preceding gene: 154686261

Following gene: 154686259

Centisome position: 48.92

GC content: 52.02

Gene sequence:

>1161_bases
GTGGATTTTGACGGATGGCTCCTCGGAAGGCTCGATGCGGTGAAAAGAGACGGGCTGTATCGGACGCTGCGAACACAGGA
AACCGCTCTCAAAACCAAAGGGCAAAAGCGGCAGACCTGGGCGTCCAATGACTATTTAGGCCTGTCAAAAGATGAGCGGC
TGATCACAGCCGCGCAAACGGCGATGAGCCGGTTCGGAGCGGGAAGCGGCGGCTCACGGTTAACGACAGGAAATACTGTG
TGGCACGAAAAACTTGAACACACAATCGCGGATTTTAAACAGACGGAAGCGGCTTTATTGTTTTCAAGCGGTTACTTGGC
AAATATCGGCGTGCTTGCCTCACTGCCTCAAAAAGGCGATGTCATTTTAAGCGATCAACTGAACCATGCGAGCATTGTTG
ACGGCTGCCGTTTATCAAAAGCGGAAACCATCGTGTACCGCCATATTGATATGGCTGATCTTGAGAAAAAACTTGCCTCC
GTCCAGGCTCGGAACCGCCGCTTCATCGTCACAGACGGCGTCTTCAGCATGGACGGCACCATCGCGCCTCTTGACCGCAT
AATGGCGCTTGCAAAGCAGTACCAGGCATTTGTGATAGCAGATGACGCGCATGCAACCGGTGTACTCGGGGAAAACGGCG
GGGGAACCAGTGACTATTTCGGTGTTTGTCCTGATGTTGTCATCGGCACATTAAGCAAAGCTGTAGGAACGGAAGGCGGT
TTTGCCGCGGGATCAAACATTTTCATTGATTTTTTGCTGAATCAGGCGCGGACGTTTATTTTTCAGACGGCCCTCCCGCC
GTCAATTTGTGCGGCTTCGCATACGGCATTTGACATCATATCAGATATGCACGATACGCGCCGTGAGCTGCAGTCGTCTG
TCAAAATGATAAAAACGCGTCTGGCCGATATGGGCTTTACCGTAAGAGGCGGAGATACACCGATTATCCCCGTTATCATC
GGTGATGCAAAAACAGCGGTTTCAGCCGCTGCACTGCTGGAGAAAAAGGGAATCTGCGCCCCGGCCATCCGCCCGCCCGC
CGTTCCGGAAGGCGAAAGCAGAATCAGGCTGACCGTTACGGCGGATCGCAGCTTACAGGATATAGACGAGCTGACAGAAG
CATTTGATTCCATTAGAAAAGAGCTGAATATAAACAAATGA

Upstream 100 bases:

>100_bases
GGAGACGTCATCGCCTTTCTGCCGCCGCTTGCCAGCACAACCGATCAGTTACAAGCCATGATCTCCATTATGAAAGAAGC
GATAGCCGAGGTGACCGGCC

Downstream 100 bases:

>100_bases
GAAGTTTTTTTGTTACAGGAACAGATACCGGCGTCGGAAAAACCATCGTTTCTTGCGGACTTGCCGCGGCATTCCGGCAG
AAACAGGCGGATATCGGAGT

Product: hypothetical protein

Products: NA

Alternate protein names: AONS; 7-keto-8-amino-pelargonic acid synthase; 7-KAP synthase; KAPA synthase; 8-amino-7-ketopelargonate synthase; L-alanine--pimeloyl-CoA ligase

Number of amino acids: Translated: 386; Mature: 386

Protein sequence:

>386_residues
MDFDGWLLGRLDAVKRDGLYRTLRTQETALKTKGQKRQTWASNDYLGLSKDERLITAAQTAMSRFGAGSGGSRLTTGNTV
WHEKLEHTIADFKQTEAALLFSSGYLANIGVLASLPQKGDVILSDQLNHASIVDGCRLSKAETIVYRHIDMADLEKKLAS
VQARNRRFIVTDGVFSMDGTIAPLDRIMALAKQYQAFVIADDAHATGVLGENGGGTSDYFGVCPDVVIGTLSKAVGTEGG
FAAGSNIFIDFLLNQARTFIFQTALPPSICAASHTAFDIISDMHDTRRELQSSVKMIKTRLADMGFTVRGGDTPIIPVII
GDAKTAVSAAALLEKKGICAPAIRPPAVPEGESRIRLTVTADRSLQDIDELTEAFDSIRKELNINK

Sequences:

>Translated_386_residues
MDFDGWLLGRLDAVKRDGLYRTLRTQETALKTKGQKRQTWASNDYLGLSKDERLITAAQTAMSRFGAGSGGSRLTTGNTV
WHEKLEHTIADFKQTEAALLFSSGYLANIGVLASLPQKGDVILSDQLNHASIVDGCRLSKAETIVYRHIDMADLEKKLAS
VQARNRRFIVTDGVFSMDGTIAPLDRIMALAKQYQAFVIADDAHATGVLGENGGGTSDYFGVCPDVVIGTLSKAVGTEGG
FAAGSNIFIDFLLNQARTFIFQTALPPSICAASHTAFDIISDMHDTRRELQSSVKMIKTRLADMGFTVRGGDTPIIPVII
GDAKTAVSAAALLEKKGICAPAIRPPAVPEGESRIRLTVTADRSLQDIDELTEAFDSIRKELNINK
>Mature_386_residues
MDFDGWLLGRLDAVKRDGLYRTLRTQETALKTKGQKRQTWASNDYLGLSKDERLITAAQTAMSRFGAGSGGSRLTTGNTV
WHEKLEHTIADFKQTEAALLFSSGYLANIGVLASLPQKGDVILSDQLNHASIVDGCRLSKAETIVYRHIDMADLEKKLAS
VQARNRRFIVTDGVFSMDGTIAPLDRIMALAKQYQAFVIADDAHATGVLGENGGGTSDYFGVCPDVVIGTLSKAVGTEGG
FAAGSNIFIDFLLNQARTFIFQTALPPSICAASHTAFDIISDMHDTRRELQSSVKMIKTRLADMGFTVRGGDTPIIPVII
GDAKTAVSAAALLEKKGICAPAIRPPAVPEGESRIRLTVTADRSLQDIDELTEAFDSIRKELNINK

Specific function: Catalyzes the decarboxylative condensation of pimeloyl- CoA and L-alanine to produce 8-amino-7-oxononanoate (AON), coenzyme A, and carbon dioxide

COG id: COG0156

COG function: function code H; 7-keto-8-aminopelargonate synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family

Homologues:

Organism=Homo sapiens, GI7657118, Length=380, Percent_Identity=38.9473684210526, Blast_Score=268, Evalue=6e-72,
Organism=Homo sapiens, GI284448556, Length=340, Percent_Identity=41.1764705882353, Blast_Score=266, Evalue=3e-71,
Organism=Homo sapiens, GI83977442, Length=394, Percent_Identity=32.741116751269, Blast_Score=210, Evalue=2e-54,
Organism=Homo sapiens, GI83977444, Length=394, Percent_Identity=32.741116751269, Blast_Score=209, Evalue=3e-54,
Organism=Homo sapiens, GI83977440, Length=394, Percent_Identity=32.741116751269, Blast_Score=209, Evalue=3e-54,
Organism=Homo sapiens, GI4502025, Length=391, Percent_Identity=33.7595907928389, Blast_Score=201, Evalue=1e-51,
Organism=Homo sapiens, GI40316939, Length=391, Percent_Identity=33.7595907928389, Blast_Score=201, Evalue=1e-51,
Organism=Homo sapiens, GI4758668, Length=359, Percent_Identity=33.7047353760446, Blast_Score=195, Evalue=6e-50,
Organism=Homo sapiens, GI119220554, Length=358, Percent_Identity=32.122905027933, Blast_Score=188, Evalue=6e-48,
Organism=Homo sapiens, GI5454084, Length=372, Percent_Identity=26.0752688172043, Blast_Score=135, Evalue=9e-32,
Organism=Escherichia coli, GI1790046, Length=393, Percent_Identity=36.3867684478371, Blast_Score=243, Evalue=2e-65,
Organism=Escherichia coli, GI1786993, Length=337, Percent_Identity=39.1691394658754, Blast_Score=217, Evalue=9e-58,
Organism=Caenorhabditis elegans, GI71994529, Length=391, Percent_Identity=40.153452685422, Blast_Score=283, Evalue=9e-77,
Organism=Caenorhabditis elegans, GI32566772, Length=361, Percent_Identity=33.2409972299169, Blast_Score=204, Evalue=8e-53,
Organism=Caenorhabditis elegans, GI17560914, Length=356, Percent_Identity=31.1797752808989, Blast_Score=169, Evalue=3e-42,
Organism=Caenorhabditis elegans, GI17560912, Length=356, Percent_Identity=30.6179775280899, Blast_Score=169, Evalue=3e-42,
Organism=Caenorhabditis elegans, GI71982625, Length=263, Percent_Identity=28.1368821292776, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI71982617, Length=263, Percent_Identity=28.1368821292776, Blast_Score=120, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6320438, Length=419, Percent_Identity=31.5035799522673, Blast_Score=202, Evalue=9e-53,
Organism=Saccharomyces cerevisiae, GI6320267, Length=364, Percent_Identity=30.4945054945055, Blast_Score=174, Evalue=3e-44,
Organism=Saccharomyces cerevisiae, GI6323954, Length=314, Percent_Identity=25.4777070063694, Blast_Score=126, Evalue=5e-30,
Organism=Drosophila melanogaster, GI24662918, Length=386, Percent_Identity=35.4922279792746, Blast_Score=254, Evalue=6e-68,
Organism=Drosophila melanogaster, GI17137420, Length=339, Percent_Identity=34.8082595870207, Blast_Score=202, Evalue=2e-52,
Organism=Drosophila melanogaster, GI17136286, Length=333, Percent_Identity=32.1321321321321, Blast_Score=169, Evalue=3e-42,
Organism=Drosophila melanogaster, GI24653280, Length=301, Percent_Identity=26.2458471760797, Blast_Score=129, Evalue=3e-30,
Organism=Drosophila melanogaster, GI24653276, Length=301, Percent_Identity=26.2458471760797, Blast_Score=129, Evalue=3e-30,
Organism=Drosophila melanogaster, GI24653278, Length=301, Percent_Identity=26.2458471760797, Blast_Score=129, Evalue=3e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOF_BACA2 (A7Z5B4)

Other databases:

- EMBL:   CP000560
- RefSeq:   YP_001421421.1
- ProteinModelPortal:   A7Z5B4
- SMR:   A7Z5B4
- STRING:   A7Z5B4
- EnsemblBacteria:   EBBACT00000006979
- GeneID:   5459523
- GenomeReviews:   CP000560_GR
- KEGG:   bay:RBAM_018270
- eggNOG:   COG0156
- GeneTree:   EBGT00050000000589
- HOGENOM:   HBG649839
- OMA:   QGIRPPT
- ProtClustDB:   CLSK887748
- BioCyc:   BAMY326423:RBAM_018270-MONOMER
- InterPro:   IPR001917
- InterPro:   IPR004839
- InterPro:   IPR004723
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- TIGRFAMs:   TIGR00858

Pfam domain/function: PF00155 Aminotran_1_2; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.3.1.47

Molecular weight: Translated: 41753; Mature: 41753

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: PS00599 AA_TRANSFER_CLASS_2

Important sites: BINDING 21-21 BINDING 129-129 BINDING 176-176

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFDGWLLGRLDAVKRDGLYRTLRTQETALKTKGQKRQTWASNDYLGLSKDERLITAAQT
CCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
AMSRFGAGSGGSRLTTGNTVWHEKLEHTIADFKQTEAALLFSSGYLANIGVLASLPQKGD
HHHHCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHEEEECCCHHHHHHHHCCCCCCC
VILSDQLNHASIVDGCRLSKAETIVYRHIDMADLEKKLASVQARNRRFIVTDGVFSMDGT
EEEECCCCCHHHHCCCCCCCHHEEEEECCCHHHHHHHHHHHHHCCCEEEEECCEEECCCC
IAPLDRIMALAKQYQAFVIADDAHATGVLGENGGGTSDYFGVCPDVVIGTLSKAVGTEGG
HHHHHHHHHHHHHCCEEEEECCCCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCC
FAAGSNIFIDFLLNQARTFIFQTALPPSICAASHTAFDIISDMHDTRRELQSSVKMIKTR
CCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LADMGFTVRGGDTPIIPVIIGDAKTAVSAAALLEKKGICAPAIRPPAVPEGESRIRLTVT
HHHCCEEEECCCCCEEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEE
ADRSLQDIDELTEAFDSIRKELNINK
CCCCHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MDFDGWLLGRLDAVKRDGLYRTLRTQETALKTKGQKRQTWASNDYLGLSKDERLITAAQT
CCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
AMSRFGAGSGGSRLTTGNTVWHEKLEHTIADFKQTEAALLFSSGYLANIGVLASLPQKGD
HHHHCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHEEEECCCHHHHHHHHCCCCCCC
VILSDQLNHASIVDGCRLSKAETIVYRHIDMADLEKKLASVQARNRRFIVTDGVFSMDGT
EEEECCCCCHHHHCCCCCCCHHEEEEECCCHHHHHHHHHHHHHCCCEEEEECCEEECCCC
IAPLDRIMALAKQYQAFVIADDAHATGVLGENGGGTSDYFGVCPDVVIGTLSKAVGTEGG
HHHHHHHHHHHHHCCEEEEECCCCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCC
FAAGSNIFIDFLLNQARTFIFQTALPPSICAASHTAFDIISDMHDTRRELQSSVKMIKTR
CCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LADMGFTVRGGDTPIIPVIIGDAKTAVSAAALLEKKGICAPAIRPPAVPEGESRIRLTVT
HHHCCEEEECCCCCEEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEE
ADRSLQDIDELTEAFDSIRKELNINK
CCCCHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA