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The map label for this gene is bioB

Identifier: 154686258

GI number: 154686258

Start: 1914125

End: 1915144

Strand: Reverse

Name: bioB

Synonym: RBAM_018250

Alternate gene names: 154686258

Gene position: 1915144-1914125 (Counterclockwise)

Preceding gene: 154686259

Following gene: 154686257

Centisome position: 48.87

GC content: 46.57

Gene sequence:

>1020_bases
ATGAATCAATGGATGGATCTTGCAGAGAGAGTGCTGGACGGGGCGGAAGTAACGAATCAAGAAGCGCTGGCCATTTTAAA
TTGTCCTGATGAGGATCTTTTATTGCTGATGCATGCCGCTTTTCATATCAGAAAACGATTTTACGGAAAAAAAGTAAAGC
TGAATATGATTATGAATGCAAAATCAGGCCTGTGTCCGGAAAATTGCGGGTACTGTTCACAATCTTCAATATCAAAAGCG
CCGATTGAGTCTTATCGGATGGTGGATAAGCATATATTGCTTAAAGGGGCAAAACGCGCCCACGACCTGAATATAGGGAC
ATACTGTATCGTCGCCAGCGGCCGGGGACCGTCAAACAGAGAAGTGGATCAAGTTGTTGATGCTGTCAAAGAAATTAAAG
AAACGTACGGTTTAAAAATCTGCGCATGCCTCGGTCTGCTGAAGCCGGAACAGGCAGAGCGGCTGAAACAGGCAGGAGTA
GACCGGTACAATCATAACATCAATACATCCCAAAGAAATCACTCAAACATTACGACCTCCCACACGTATGACGACAGGGT
CAATACAGTGGAAACGGCGAAACAATCGGGGATGTCACCTTGTTCTGGTGTCATCGTCGGCATGAAAGAGACGAAACAGG
ACGTCATTGATATGGCGAACAGCTTAAAAGCGCTTGACGCCGACAGCATCCCGGTTAATTTTCTGCATGCGATTGACGGA
ACTCCGTTAGAAGGGGTAAGCGAATTACATCCGGTGTATTGCCTGAAAGTGCTCGCCTTGTTCCGTTTTATCAATCCGTC
AAAAGAAATCCGTATCTCAGGAGGCCGGGAAGTCAACCTTCGTTCGCTGCAGCCGCTGGGACTTTACGCCGCAAATTCGA
TTTTCGTCGGAGATTACTTAACGACAGCAGGGCAGCAAGAAACAGAGGATCATCAAATGCTCCATGATTTAGGTTTTGAA
GTCGAATCCGTTGAAGAAATGAAAGCCGGCCTGCAATCCGCCTGCAATACGGAGGTATAA

Upstream 100 bases:

>100_bases
CGAAGCTGGATCACGTTTCATCGGAAAACATTCAACACATGATTGAGAAACATATAGATGTTTCCTCTTTCATGAATCTG
ACACAAATGGGGAGATAAAC

Downstream 100 bases:

>100_bases
GATGACGGCGGACCTATCGATTGCACATCCGTCTCTCCATGCGGATTCGGGTTTTTGGAATGACCCTTACCCGTTTTATG
ACAAACTGCGATCCATTGAT

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 339; Mature: 339

Protein sequence:

>339_residues
MNQWMDLAERVLDGAEVTNQEALAILNCPDEDLLLLMHAAFHIRKRFYGKKVKLNMIMNAKSGLCPENCGYCSQSSISKA
PIESYRMVDKHILLKGAKRAHDLNIGTYCIVASGRGPSNREVDQVVDAVKEIKETYGLKICACLGLLKPEQAERLKQAGV
DRYNHNINTSQRNHSNITTSHTYDDRVNTVETAKQSGMSPCSGVIVGMKETKQDVIDMANSLKALDADSIPVNFLHAIDG
TPLEGVSELHPVYCLKVLALFRFINPSKEIRISGGREVNLRSLQPLGLYAANSIFVGDYLTTAGQQETEDHQMLHDLGFE
VESVEEMKAGLQSACNTEV

Sequences:

>Translated_339_residues
MNQWMDLAERVLDGAEVTNQEALAILNCPDEDLLLLMHAAFHIRKRFYGKKVKLNMIMNAKSGLCPENCGYCSQSSISKA
PIESYRMVDKHILLKGAKRAHDLNIGTYCIVASGRGPSNREVDQVVDAVKEIKETYGLKICACLGLLKPEQAERLKQAGV
DRYNHNINTSQRNHSNITTSHTYDDRVNTVETAKQSGMSPCSGVIVGMKETKQDVIDMANSLKALDADSIPVNFLHAIDG
TPLEGVSELHPVYCLKVLALFRFINPSKEIRISGGREVNLRSLQPLGLYAANSIFVGDYLTTAGQQETEDHQMLHDLGFE
VESVEEMKAGLQSACNTEV
>Mature_339_residues
MNQWMDLAERVLDGAEVTNQEALAILNCPDEDLLLLMHAAFHIRKRFYGKKVKLNMIMNAKSGLCPENCGYCSQSSISKA
PIESYRMVDKHILLKGAKRAHDLNIGTYCIVASGRGPSNREVDQVVDAVKEIKETYGLKICACLGLLKPEQAERLKQAGV
DRYNHNINTSQRNHSNITTSHTYDDRVNTVETAKQSGMSPCSGVIVGMKETKQDVIDMANSLKALDADSIPVNFLHAIDG
TPLEGVSELHPVYCLKVLALFRFINPSKEIRISGGREVNLRSLQPLGLYAANSIFVGDYLTTAGQQETEDHQMLHDLGFE
VESVEEMKAGLQSACNTEV

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family

Homologues:

Organism=Escherichia coli, GI1786992, Length=298, Percent_Identity=34.5637583892617, Blast_Score=179, Evalue=3e-46,
Organism=Saccharomyces cerevisiae, GI6321725, Length=304, Percent_Identity=34.2105263157895, Blast_Score=159, Evalue=5e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOB_BACA2 (A7Z5B2)

Other databases:

- EMBL:   CP000560
- RefSeq:   YP_001421419.1
- ProteinModelPortal:   A7Z5B2
- SMR:   A7Z5B2
- STRING:   A7Z5B2
- EnsemblBacteria:   EBBACT00000006582
- GeneID:   5459521
- GenomeReviews:   CP000560_GR
- KEGG:   bay:RBAM_018250
- eggNOG:   COG0502
- GeneTree:   EBGT00050000002917
- HOGENOM:   HBG405569
- OMA:   MGMETCM
- ProtClustDB:   PRK06256
- BioCyc:   BAMY326423:RBAM_018250-MONOMER
- HAMAP:   MF_01694
- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00876
- SMART:   SM00729
- TIGRFAMs:   TIGR00433

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM

EC number: =2.8.1.6

Molecular weight: Translated: 37483; Mature: 37483

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQWMDLAERVLDGAEVTNQEALAILNCPDEDLLLLMHAAFHIRKRFYGKKVKLNMIMNA
CCHHHHHHHHHHCCHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEECC
KSGLCPENCGYCSQSSISKAPIESYRMVDKHILLKGAKRAHDLNIGTYCIVASGRGPSNR
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHCCCCCCEEEEEECCCCCCCC
EVDQVVDAVKEIKETYGLKICACLGLLKPEQAERLKQAGVDRYNHNINTSQRNHSNITTS
CHHHHHHHHHHHHHHHCHHHHHHHCCCCCHHHHHHHHHCCHHHCCCCCCCCCCCCCCCCC
HTYDDRVNTVETAKQSGMSPCSGVIVGMKETKQDVIDMANSLKALDADSIPVNFLHAIDG
CCCCCHHHHHHHHHHCCCCCCHHEEECHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCC
TPLEGVSELHPVYCLKVLALFRFINPSKEIRISGGREVNLRSLQPLGLYAANSIFVGDYL
CCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCEECCCCCCCCCCEECCCEEECHHH
TTAGQQETEDHQMLHDLGFEVESVEEMKAGLQSACNTEV
HCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MNQWMDLAERVLDGAEVTNQEALAILNCPDEDLLLLMHAAFHIRKRFYGKKVKLNMIMNA
CCHHHHHHHHHHCCHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEECC
KSGLCPENCGYCSQSSISKAPIESYRMVDKHILLKGAKRAHDLNIGTYCIVASGRGPSNR
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHCCCCCCEEEEEECCCCCCCC
EVDQVVDAVKEIKETYGLKICACLGLLKPEQAERLKQAGVDRYNHNINTSQRNHSNITTS
CHHHHHHHHHHHHHHHCHHHHHHHCCCCCHHHHHHHHHCCHHHCCCCCCCCCCCCCCCCC
HTYDDRVNTVETAKQSGMSPCSGVIVGMKETKQDVIDMANSLKALDADSIPVNFLHAIDG
CCCCCHHHHHHHHHHCCCCCCHHEEECHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCC
TPLEGVSELHPVYCLKVLALFRFINPSKEIRISGGREVNLRSLQPLGLYAANSIFVGDYL
CCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCEECCCCCCCCCCEECCCEEECHHH
TTAGQQETEDHQMLHDLGFEVESVEEMKAGLQSACNTEV
HCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA