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The map label for this gene is ebrA [H]

Identifier: 154686144

GI number: 154686144

Start: 1778514

End: 1778828

Strand: Reverse

Name: ebrA [H]

Synonym: RBAM_017110

Alternate gene names: 154686144

Gene position: 1778828-1778514 (Counterclockwise)

Preceding gene: 154686154

Following gene: 154686143

Centisome position: 45.39

GC content: 47.94

Gene sequence:

>315_bases
ATGATTCTGGGTTATATCTTCCTCACAATTGCTATTTTATCAGAGTCAATCGGCGCGGCCATGCTGAAGGTCTCCCGCGG
ATTTACGCGGTTTTTGCCGAGCGCGCTTGTAGTGGCAGGCTATTCATTGGCCTTTTATATGCTTTCACTGACACTGAATC
TCATTCCGCTCAGCTTATCCTATGCAACATGGAGCGGAGCCGGAACGGTGCTCGCATCCATTATCGGAGCGAAATGGTTT
CATGAAAAACTGGACAAGCAGGCCGTCATCGGACTCTTTTTCTTATTGGCCGGTGTCATTTTGATGAATTGGTAG

Upstream 100 bases:

>100_bases
AGGCCTAATGTCTTGACTTGGCGGGCAAAGTTCGGATATTATTTTATTTTACTGCCTTTAAACAAAAGGCATAAGGATTA
TGATGAGAGGGGGATTTGAT

Downstream 100 bases:

>100_bases
AAAGGAGAGGCATGCTGATGAAAGGATTACTTTGCTTGGCATTAGCCATTGTATCAGAAGTGTTCGGGAGCACGATGCTG
AAGCTGTCGGACGGTTTTAC

Product: EbrA

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 104; Mature: 104

Protein sequence:

>104_residues
MILGYIFLTIAILSESIGAAMLKVSRGFTRFLPSALVVAGYSLAFYMLSLTLNLIPLSLSYATWSGAGTVLASIIGAKWF
HEKLDKQAVIGLFFLLAGVILMNW

Sequences:

>Translated_104_residues
MILGYIFLTIAILSESIGAAMLKVSRGFTRFLPSALVVAGYSLAFYMLSLTLNLIPLSLSYATWSGAGTVLASIIGAKWF
HEKLDKQAVIGLFFLLAGVILMNW
>Mature_104_residues
MILGYIFLTIAILSESIGAAMLKVSRGFTRFLPSALVVAGYSLAFYMLSLTLNLIPLSLSYATWSGAGTVLASIIGAKWF
HEKLDKQAVIGLFFLLAGVILMNW

Specific function: Part of a multidrug efflux pump. Confers resistance to cationic lipophilic dyes such as ethidium bromide, acriflavine, pyronine Y and safranin O. The efflux is probably coupled to an influx of protons [H]

COG id: COG2076

COG function: function code P; Membrane transporters of cations and cationic drugs

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the small multidrug resistance (SMR) protein family. EbrA/ebrB (TC 2.A.7.1.6) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786755, Length=99, Percent_Identity=43.4343434343434, Blast_Score=86, Evalue=7e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000390 [H]

Pfam domain/function: PF00893 Multi_Drug_Res [H]

EC number: NA

Molecular weight: Translated: 11290; Mature: 11290

Theoretical pI: Translated: 9.91; Mature: 9.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILGYIFLTIAILSESIGAAMLKVSRGFTRFLPSALVVAGYSLAFYMLSLTLNLIPLSLS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YATWSGAGTVLASIIGAKWFHEKLDKQAVIGLFFLLAGVILMNW
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MILGYIFLTIAILSESIGAAMLKVSRGFTRFLPSALVVAGYSLAFYMLSLTLNLIPLSLS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YATWSGAGTVLASIIGAKWFHEKLDKQAVIGLFFLLAGVILMNW
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10735876; 9384377 [H]