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The map label for this gene is ebrB [H]

Identifier: 154686143

GI number: 154686143

Start: 1778143

End: 1778496

Strand: Reverse

Name: ebrB [H]

Synonym: RBAM_017100

Alternate gene names: 154686143

Gene position: 1778496-1778143 (Counterclockwise)

Preceding gene: 154686144

Following gene: 154686140

Centisome position: 45.39

GC content: 48.59

Gene sequence:

>354_bases
ATGAAAGGATTACTTTGCTTGGCATTAGCCATTGTATCAGAAGTGTTCGGGAGCACGATGCTGAAGCTGTCGGACGGTTT
TACGCACCTGTGGCCCGCTCTCGGCGTCATTGCCGGAATCGGAGCCTCTTTTATGTTTTTGAGCTTCTCATTGAAAAGCC
TTGATTTATCCGCCGCATACGCGACATGGTCAGGTGTCGGCACCGCTTTAACGGCAATTGTCGGCTTTTTTGTTTTTAAG
GAGGCCATGACAGTCAAAATGATGATCGGGCTTGTTTTGGTCATCACGGGCGTCATCCTGCTGAACCGTTCCAAGTCTCA
AAAAGCTAAAAAAAATCAAACGGCCCGGAGCTGA

Upstream 100 bases:

>100_bases
AATGGTTTCATGAAAAACTGGACAAGCAGGCCGTCATCGGACTCTTTTTCTTATTGGCCGGTGTCATTTTGATGAATTGG
TAGAAAGGAGAGGCATGCTG

Downstream 100 bases:

>100_bases
CCGTTTGAACTCGTCAAGCGCGGGCAGCTTTTACTTCAACCTCAAGCTCCATCTCCACGTTTCCTTGGACCGCTCGTGAA
ATCATACATGAGGACTCTGC

Product: EbrB

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 117; Mature: 117

Protein sequence:

>117_residues
MKGLLCLALAIVSEVFGSTMLKLSDGFTHLWPALGVIAGIGASFMFLSFSLKSLDLSAAYATWSGVGTALTAIVGFFVFK
EAMTVKMMIGLVLVITGVILLNRSKSQKAKKNQTARS

Sequences:

>Translated_117_residues
MKGLLCLALAIVSEVFGSTMLKLSDGFTHLWPALGVIAGIGASFMFLSFSLKSLDLSAAYATWSGVGTALTAIVGFFVFK
EAMTVKMMIGLVLVITGVILLNRSKSQKAKKNQTARS
>Mature_117_residues
MKGLLCLALAIVSEVFGSTMLKLSDGFTHLWPALGVIAGIGASFMFLSFSLKSLDLSAAYATWSGVGTALTAIVGFFVFK
EAMTVKMMIGLVLVITGVILLNRSKSQKAKKNQTARS

Specific function: Part of a multidrug efflux pump. Confers resistance to cationic lipophilic dyes such as ethidium bromide, acriflavine, pyronine Y and safranin O. The efflux is probably coupled to an influx of protons [H]

COG id: COG2076

COG function: function code P; Membrane transporters of cations and cationic drugs

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the small multidrug resistance (SMR) protein family. EbrA/ebrB (TC 2.A.7.1.6) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786755, Length=102, Percent_Identity=38.2352941176471, Blast_Score=77, Evalue=2e-16,
Organism=Escherichia coli, GI1787883, Length=96, Percent_Identity=42.7083333333333, Blast_Score=60, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000390 [H]

Pfam domain/function: PF00893 Multi_Drug_Res [H]

EC number: NA

Molecular weight: Translated: 12407; Mature: 12407

Theoretical pI: Translated: 10.74; Mature: 10.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGLLCLALAIVSEVFGSTMLKLSDGFTHLWPALGVIAGIGASFMFLSFSLKSLDLSAAY
CCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ATWSGVGTALTAIVGFFVFKEAMTVKMMIGLVLVITGVILLNRSKSQKAKKNQTARS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCC
>Mature Secondary Structure
MKGLLCLALAIVSEVFGSTMLKLSDGFTHLWPALGVIAGIGASFMFLSFSLKSLDLSAAY
CCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ATWSGVGTALTAIVGFFVFKEAMTVKMMIGLVLVITGVILLNRSKSQKAKKNQTARS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10735876; 9384377 [H]