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The map label for this gene is cwlC [H]

Identifier: 154686154

GI number: 154686154

Start: 1786194

End: 1786952

Strand: Reverse

Name: cwlC [H]

Synonym: RBAM_017210

Alternate gene names: 154686154

Gene position: 1786952-1786194 (Counterclockwise)

Preceding gene: 154686160

Following gene: 154686144

Centisome position: 45.6

GC content: 48.48

Gene sequence:

>759_bases
ATGGTTAAAATTTTTATAGACCCCGGTCATGGCGGAACTGATTCAGGTGCAACCGGAAACGGACTTCAGGAAAAAGCGGT
CACGCTGGAGATCGCGCTGGCTTTGCGCACGATTCTGACGAATGAGTATGAAGGAGTTTCTGTCACGATGAGCAGGACAA
GCGATAAGACAGTCAGCCTGACGGAGAGAACGGATGCCGCCAACAGCTGGGGCGCAGATTATTTTTTATCCATTCATATT
AACTCAGGAGGCGGAACCGGATTCGAAAGCTATATTTACCCCGGTGTCGGAGCGCCGACCTCGACTTACCAATCAGCCGT
CCACACGCAAGTGATTCAAGCATCGGGTTTTAATGACCGAGGAAAAAAAACAGCCAACTTCCATGTGCTGCGGGAGACAA
GTATGCCGGCGATTTTGACGGAGAATGGTTTTGTTGATACGTCAGCTGATGCAAACAAACTGAAAACAGCCAGTTTTATT
GAAAGCCTTGCAAGAGGACATGCCAAGGGCTTGGAGCAAGCTTTCGGTCTAAAAAAAAAGGCTGACTTATATAAAGTGCA
AATCGGTGCATTCAAAGTGAAAGCAAATGCTGATGCGCTTGCCGCCGATGCGGAGTCTAAAGGTTTTGACGCGATTGTGC
TTCCAAAAGGCGGCTTATTTAAAGTGCAGATCGGCGCATTCTCTTCCAGAGATTACGCTGAAGCGTTGGCCGCGCGGGCC
GAAAAAGCCGGATTTGATGCCTTCATTATGCAAGACTAA

Upstream 100 bases:

>100_bases
TTTCCGGGAAATGTTGGGGATATTTCTCATTTTTTTTATGAATGACAGCCGTACAAGCACCATTTGAATATCATCATATG
CTGTAATGAGGTGATGGAGA

Downstream 100 bases:

>100_bases
ATGAAAAAACCCCTGCTTTCCCTTTTTTGGGACGAACGGGGGTTTTTTGTTATTGAAGTATATCGGCGACAAACTGAGAC
CAAGCTTCAAGACGCTGATA

Product: CwlC

Products: NA

Alternate protein names: Autolysin; Cell wall hydrolase [H]

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MVKIFIDPGHGGTDSGATGNGLQEKAVTLEIALALRTILTNEYEGVSVTMSRTSDKTVSLTERTDAANSWGADYFLSIHI
NSGGGTGFESYIYPGVGAPTSTYQSAVHTQVIQASGFNDRGKKTANFHVLRETSMPAILTENGFVDTSADANKLKTASFI
ESLARGHAKGLEQAFGLKKKADLYKVQIGAFKVKANADALAADAESKGFDAIVLPKGGLFKVQIGAFSSRDYAEALAARA
EKAGFDAFIMQD

Sequences:

>Translated_252_residues
MVKIFIDPGHGGTDSGATGNGLQEKAVTLEIALALRTILTNEYEGVSVTMSRTSDKTVSLTERTDAANSWGADYFLSIHI
NSGGGTGFESYIYPGVGAPTSTYQSAVHTQVIQASGFNDRGKKTANFHVLRETSMPAILTENGFVDTSADANKLKTASFI
ESLARGHAKGLEQAFGLKKKADLYKVQIGAFKVKANADALAADAESKGFDAIVLPKGGLFKVQIGAFSSRDYAEALAARA
EKAGFDAFIMQD
>Mature_252_residues
MVKIFIDPGHGGTDSGATGNGLQEKAVTLEIALALRTILTNEYEGVSVTMSRTSDKTVSLTERTDAANSWGADYFLSIHI
NSGGGTGFESYIYPGVGAPTSTYQSAVHTQVIQASGFNDRGKKTANFHVLRETSMPAILTENGFVDTSADANKLKTASFI
ESLARGHAKGLEQAFGLKKKADLYKVQIGAFKVKANADALAADAESKGFDAIVLPKGGLFKVQIGAFSSRDYAEALAARA
EKAGFDAFIMQD

Specific function: Autolysins are involved in some important biological processes such as cell separation, cell-wall turnover, competence for genetic transformation, formation of the flagella - in particular of its basal body - and sporulation. CwlC is able to hydrolyze typ

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Secreted, cell wall. Note=Is associated with the mother cell wall of sporulating cells [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788776, Length=220, Percent_Identity=27.2727272727273, Blast_Score=78, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002508
- InterPro:   IPR007730 [H]

Pfam domain/function: PF01520 Amidase_3; PF05036 SPOR [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 26672; Mature: 26672

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKIFIDPGHGGTDSGATGNGLQEKAVTLEIALALRTILTNEYEGVSVTMSRTSDKTVSL
CEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEE
TERTDAANSWGADYFLSIHINSGGGTGFESYIYPGVGAPTSTYQSAVHTQVIQASGFNDR
EECCCCCCCCCCEEEEEEEEECCCCCCCHHEECCCCCCCHHHHHHHHHHHHHCCCCCCCC
GKKTANFHVLRETSMPAILTENGFVDTSADANKLKTASFIESLARGHAKGLEQAFGLKKK
CCCCCEEEEEECCCCCEEEECCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
ADLYKVQIGAFKVKANADALAADAESKGFDAIVLPKGGLFKVQIGAFSSRDYAEALAARA
CCEEEEEEEEEEEECCCCCEECCCCCCCCCEEEECCCCEEEEEECCCCCCHHHHHHHHHH
EKAGFDAFIMQD
HHCCCEEEEECC
>Mature Secondary Structure
MVKIFIDPGHGGTDSGATGNGLQEKAVTLEIALALRTILTNEYEGVSVTMSRTSDKTVSL
CEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEE
TERTDAANSWGADYFLSIHINSGGGTGFESYIYPGVGAPTSTYQSAVHTQVIQASGFNDR
EECCCCCCCCCCEEEEEEEEECCCCCCCHHEECCCCCCCHHHHHHHHHHHHHCCCCCCCC
GKKTANFHVLRETSMPAILTENGFVDTSADANKLKTASFIESLARGHAKGLEQAFGLKKK
CCCCCEEEEEECCCCCEEEECCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
ADLYKVQIGAFKVKANADALAADAESKGFDAIVLPKGGLFKVQIGAFSSRDYAEALAARA
CCEEEEEEEEEEEECCCCCEECCCCCCCCCEEEECCCCEEEEEECCCCCCHHHHHHHHHH
EKAGFDAFIMQD
HHCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8407798; 9384377 [H]