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The map label for this gene is ispA [H]

Identifier: 154685738

GI number: 154685738

Start: 1267969

End: 1268928

Strand: Reverse

Name: ispA [H]

Synonym: RBAM_013050

Alternate gene names: 154685738

Gene position: 1268928-1267969 (Counterclockwise)

Preceding gene: 154685740

Following gene: 154685737

Centisome position: 32.38

GC content: 50.62

Gene sequence:

>960_bases
ATGAATGGTGAAATGCATTTGATTCCTTATGTCACGGATGAACAGATCATGGATGTGAACGAGCTTCCTGAGGGGATTAA
AGTGATTAAGGCGCCAGAGTTGTGGGCAAAAGGGTTTAAAGGCAAGGATATTAAAATCGCGGTGCTTGACACAGGCTGCG
ACATCAATCATCCTGATTTGAAAGACCGGATTGTCGGCGGGAAAAACTTCACCGATGATGACGGAGGCAAAGAAGACGCG
ATTTCCGATTATAACGGACACGGGACACACGTTTCCGGAACGATTGCCGCCAATGATTCAAACGGCGGAATTTCGGGTGT
TGCGCCGGAAGCAAGCCTTTTAATCGTTAAAGTTCTCGGCGGTCAAAACGGCAGCGGCAAATACGAATGGATCATTAACG
GCATCAACTACGCCGTTGAACAAAAGGCAGATATCATTTCCATGTCACTCGGCGGGCCAAGCGATGTGCCTGAATTGAAA
GAAGCCGTCACAAATGCGGTAAAAAGCGGTGTTCTGGTCGTTTGCGCGGCAGGAAACGAAGGCGACGGAGACGAGCGTAC
AGAGGAACTGTCGTATCCGGCCGCTTACAACGAAGTGATCGCAGTCGGATCAGTCTCAATCGCTCGTAAATCGTCTGAAT
TTTCAAACGCAAATAAAGAGATTGACCTGGTGGCTCCGGGAGAAAATATTCTCTCCACCCTTCCCAACCATAAATACGGG
AAACTGACGGGCACTTCAATGGCCGCCCCGCATGTGAGCGGAGCGCTTGCCTTAATAAAAGGGCTTGAACAGGACGCGTT
TCAGCGGACGCTTTCTGAGGCTGAAGTCTACGCCCAGCTCGTGCGGCGGACCCTTCCTTTGGACATCGCCAAAACATTGG
CCGGGAACGGGTTTCTGTATTTAGACGCGCCTGAAGAGCTGATGGAAAGAGCCGAACAGGCACAGCTGCTGTCTTTCTGA

Upstream 100 bases:

>100_bases
AAAATGCTTCAAATGACTCTTTCCGGCCGGCATGCTCTGCCATAAAATAGTAGCAACACCCTTGTATCCACTCGCAAGCA
TAAAAAAGGAGGGCTTTTTT

Downstream 100 bases:

>100_bases
TATTTTTCTCATATAATGATTGATCAGCTTGTCACATCACCAATTTTTAAAATGTGAGATTTTCTATTGACAGTGATCTG
AATAAGCTGATAAGATAACC

Product: hypothetical protein

Products: NA

Alternate protein names: ISP-1 [H]

Number of amino acids: Translated: 319; Mature: 319

Protein sequence:

>319_residues
MNGEMHLIPYVTDEQIMDVNELPEGIKVIKAPELWAKGFKGKDIKIAVLDTGCDINHPDLKDRIVGGKNFTDDDGGKEDA
ISDYNGHGTHVSGTIAANDSNGGISGVAPEASLLIVKVLGGQNGSGKYEWIINGINYAVEQKADIISMSLGGPSDVPELK
EAVTNAVKSGVLVVCAAGNEGDGDERTEELSYPAAYNEVIAVGSVSIARKSSEFSNANKEIDLVAPGENILSTLPNHKYG
KLTGTSMAAPHVSGALALIKGLEQDAFQRTLSEAEVYAQLVRRTLPLDIAKTLAGNGFLYLDAPEELMERAEQAQLLSF

Sequences:

>Translated_319_residues
MNGEMHLIPYVTDEQIMDVNELPEGIKVIKAPELWAKGFKGKDIKIAVLDTGCDINHPDLKDRIVGGKNFTDDDGGKEDA
ISDYNGHGTHVSGTIAANDSNGGISGVAPEASLLIVKVLGGQNGSGKYEWIINGINYAVEQKADIISMSLGGPSDVPELK
EAVTNAVKSGVLVVCAAGNEGDGDERTEELSYPAAYNEVIAVGSVSIARKSSEFSNANKEIDLVAPGENILSTLPNHKYG
KLTGTSMAAPHVSGALALIKGLEQDAFQRTLSEAEVYAQLVRRTLPLDIAKTLAGNGFLYLDAPEELMERAEQAQLLSF
>Mature_319_residues
MNGEMHLIPYVTDEQIMDVNELPEGIKVIKAPELWAKGFKGKDIKIAVLDTGCDINHPDLKDRIVGGKNFTDDDGGKEDA
ISDYNGHGTHVSGTIAANDSNGGISGVAPEASLLIVKVLGGQNGSGKYEWIINGINYAVEQKADIISMSLGGPSDVPELK
EAVTNAVKSGVLVVCAAGNEGDGDERTEELSYPAAYNEVIAVGSVSIARKSSEFSNANKEIDLVAPGENILSTLPNHKYG
KLTGTSMAAPHVSGALALIKGLEQDAFQRTLSEAEVYAQLVRRTLPLDIAKTLAGNGFLYLDAPEELMERAEQAQLLSF

Specific function: Major intracellular protease produced by Bacillus subtilis [H]

COG id: COG1404

COG function: function code O; Subtilisin-like serine proteases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S8 family [H]

Homologues:

Organism=Homo sapiens, GI4506775, Length=247, Percent_Identity=30.3643724696356, Blast_Score=96, Evalue=5e-20,
Organism=Homo sapiens, GI31317307, Length=262, Percent_Identity=26.3358778625954, Blast_Score=65, Evalue=8e-11,
Organism=Saccharomyces cerevisiae, GI6320775, Length=285, Percent_Identity=35.0877192982456, Blast_Score=120, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6324576, Length=282, Percent_Identity=32.2695035460993, Blast_Score=104, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6319893, Length=265, Percent_Identity=31.6981132075472, Blast_Score=101, Evalue=2e-22,
Organism=Drosophila melanogaster, GI45550681, Length=256, Percent_Identity=31.640625, Blast_Score=103, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000209
- InterPro:   IPR022398
- InterPro:   IPR015500 [H]

Pfam domain/function: PF00082 Peptidase_S8 [H]

EC number: NA

Molecular weight: Translated: 33852; Mature: 33852

Theoretical pI: Translated: 4.39; Mature: 4.39

Prosite motif: PS00136 SUBTILASE_ASP ; PS00137 SUBTILASE_HIS ; PS00138 SUBTILASE_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNGEMHLIPYVTDEQIMDVNELPEGIKVIKAPELWAKGFKGKDIKIAVLDTGCDINHPDL
CCCCEEEEEEECCHHHCCHHHCCCCCEEEECCHHHHCCCCCCCEEEEEEECCCCCCCCCH
KDRIVGGKNFTDDDGGKEDAISDYNGHGTHVSGTIAANDSNGGISGVAPEASLLIVKVLG
HHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCEEEEEEEEC
GQNGSGKYEWIINGINYAVEQKADIISMSLGGPSDVPELKEAVTNAVKSGVLVVCAAGNE
CCCCCCEEEEEEECCCHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHCCCEEEEEECCCC
GDGDERTEELSYPAAYNEVIAVGSVSIARKSSEFSNANKEIDLVAPGENILSTLPNHKYG
CCCCHHHHHCCCCCHHHHEEEECCHHHHHCCHHHCCCCCEEEEEECCHHHHHHCCCCCCC
KLTGTSMAAPHVSGALALIKGLEQDAFQRTLSEAEVYAQLVRRTLPLDIAKTLAGNGFLY
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCEEE
LDAPEELMERAEQAQLLSF
ECCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNGEMHLIPYVTDEQIMDVNELPEGIKVIKAPELWAKGFKGKDIKIAVLDTGCDINHPDL
CCCCEEEEEEECCHHHCCHHHCCCCCEEEECCHHHHCCCCCCCEEEEEEECCCCCCCCCH
KDRIVGGKNFTDDDGGKEDAISDYNGHGTHVSGTIAANDSNGGISGVAPEASLLIVKVLG
HHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCEEEEEEEEC
GQNGSGKYEWIINGINYAVEQKADIISMSLGGPSDVPELKEAVTNAVKSGVLVVCAAGNE
CCCCCCEEEEEEECCCHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHCCCEEEEEECCCC
GDGDERTEELSYPAAYNEVIAVGSVSIARKSSEFSNANKEIDLVAPGENILSTLPNHKYG
CCCCHHHHHCCCCCHHHHEEEECCHHHHHCCHHHCCCCCEEEEEECCHHHHHHCCCCCCC
KLTGTSMAAPHVSGALALIKGLEQDAFQRTLSEAEVYAQLVRRTLPLDIAKTLAGNGFLY
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCEEE
LDAPEELMERAEQAQLLSF
ECCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3087947; 9384377 [H]