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The map label for this gene is metE

Identifier: 154685737

GI number: 154685737

Start: 1265303

End: 1267591

Strand: Reverse

Name: metE

Synonym: RBAM_013040

Alternate gene names: 154685737

Gene position: 1267591-1265303 (Counterclockwise)

Preceding gene: 154685738

Following gene: 154685735

Centisome position: 32.35

GC content: 47.31

Gene sequence:

>2289_bases
ATGACAACAATCAAAACTTCGAACCTCGGTTTTCCGAGAATCGGCTTGAACAGAGAATGGAAAAAAACGCTTGAGGCTTA
TTGGAAAGGTAACACTGACAAAGAAACGTTTCTTAAAGAGCTTGACGGTTTATTCTTATCAGCTGTCAAAACACAGCTTG
ACCAACATATCGATATCGTGCCGGTCTCTGATTTCACCCATTATGACCATGTGCTTGATACAGCCGTCAGCTTTAACTGG
ATTCCGAAAAGATTCCGCAGCATCACGGATCCCGTTGATACATACTTTGCGATTGCCCGCGGTGTAAAAGACGCCGTTTC
CAGTGAAATGACGAAATGGTTTAATACCAACTACCATTACATCGTTCCTGAATATGACGAAAGCATTGAATTCCGTCTGA
CGAGAAACAAACAGCTTGACGACTACCGCCGGATCAAAGAAGAATTCGGAGCGGAAACGAAACCTGTCATCGTCGGTCCG
TATACATTTGTCACGCTTGCGAAAGGATACGAAGAATCAGAAGCGAAAGCGATTCAAAAACGCCTTGTTCCTCTTTATGT
TCAGCTGCTGAAAGAACTTGAAGCTGAAGGCGTAAAATGGGTTCAGATCGATGAGCCGGCCCTTGTGACAGCTTCTTCTG
AAGACGTCCGTGCCGCAAAAGAACTCTATGAATCTATTACAAAAGAGCTTTCAGGGCTGAATGTCCTCCTGCAGACATAC
TTCGATTCGGTTGATGCATATGAAGAACTCGTTTCGTATCCTGTACAGGGAATCGGGCTTGACTTCGTACATGATAAAGG
CAGAAACCTTGATCAGCTGAAAAAGCACGGCTTCCCGAAAGATAAAGTGCTGGCAGCCGGTGTGATCGACGGCCGAAACA
TTTGGAAAATCGATGTTGAAGAACGCCTTGACGCGGCTCTGGATATCCTCAGCCACGCTGATGTTGAGGAGTTATGGATT
CAGCCTTCAAGCAGCCTTCTGCACGTGCCGGTGGCGAAACACCCTGACGAGCATCTTGAAAAGGATTTGCTGAACGGCCT
GTCATACGCTAAAGAAAAACTGGCGGAACTCGGCGTCTTAAAAGAAGGGCTTCTGTCCGGCAAAGCGGCCGTCAGTGAGC
AGATCGAAGAATCAAAAGCGGCACTTAAGGCTTTAAAAGCATTCGCAACAGGAGCGAACTCAGCTCAAAAAGAAGAGCTG
AATCAGCTGACGGAAAAAGATTTCTCCCGTCCGGCCAGCTTTGAAGAACGATTGGCGCTGCAGAATGAATCACTCGGTCT
CCCCCTTCTGCCGACGACGACAATCGGAAGTTTCCCTCAATCTGCAGAGGTGCGGAGCGCACGGCAAAAATGGCGAAAAA
AAGATTGGACGGATGAACAGTACCAAGCCTTTATTAATGCGGAAACGAAGCGCTGGGTTGACATTCAGGAAGAAATCGGC
TTAGACGTGCTCGTTCACGGAGAATTCGAGCGGACGGACATGGTCGAATACTTCGGTGAAAAACTGGCCGGATTCGCCTT
TACGAAATATGCATGGGTGCAGTCTTACGGTTCACGCTGCGTACGTCCGCCTGTCATTTTCGGAGATGTCGAATTTATCG
AACCGATGACGGTAAAAGATACGGTCTACGCCCAATCGCTGACGGAAAAACACATGAAAGGCATGCTGACGGGACCTGTC
ACCATCTTAAACTGGTCATTCCCGCGTGTGGATATTTCAAGAAAAGAAATCGCCTTCCAGATCGGGCTTGCCCTCAGAAA
AGAAGTAAAAGCGCTTGAAGATGCCGGTATTCAGATTATCCAAGTGGATGAACCCGCGCTCCGCGAAGGACTGCCGTTAA
AAACGCAGGATTGGGATGAATATTTAACTTGGGCCGCAGAAGCATTCCGGTTAACGACGTCTTCTGTGCAAAATGAGACG
CAGATTCATACGCATATGTGTTACAGCAACTTTGAAGATATCGTAGATACGATCAATGATCTTGACGCGGATGTCATTAC
AATCGAACACAGCAGAAGCCACGGAGGATTTTTAGAATATTTGAAAGAACATCCTTACGTGAAAGGCCTCGGACTCGGTG
TATACGACATTCACAGCCCGCGTGTGCCGGCAACTGAAGAAATCTATCAAATTATTGATGACGCGCTTGAAGTGTGTCCG
ACTGACCGCTTCTGGGTAAACCCGGACTGCGGACTGAAAACAAGACAGCAGGAAGAAACCGTTGCGGCATTAAAAAATAT
GGTCGACGCCGCGAAACAGGCACGAAAAAAACAAGCTCAGCTTGTATAA

Upstream 100 bases:

>100_bases
GATCTGAGAGATAAGAGAGGCTTACATTTTGAAGTTAAAGCCTCTTTCTCTCATGAGAAAGAGGCTTTTTTGTTACATCT
CACTTAAAGGAGGAAACTTA

Downstream 100 bases:

>100_bases
AAAAACAGCCATCCGTAACTTGCGGGTGGCTGTTTTTTCTGGGCGTGCTATTTATTCTTCAGCGCTTCTGCAAGTTCATA
TAATGAATCATACCGCTTAA

Product: 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase

Products: NA

Alternate protein names: Cobalamin-independent methionine synthase; Methionine synthase, vitamin-B12 independent isozyme

Number of amino acids: Translated: 762; Mature: 761

Protein sequence:

>762_residues
MTTIKTSNLGFPRIGLNREWKKTLEAYWKGNTDKETFLKELDGLFLSAVKTQLDQHIDIVPVSDFTHYDHVLDTAVSFNW
IPKRFRSITDPVDTYFAIARGVKDAVSSEMTKWFNTNYHYIVPEYDESIEFRLTRNKQLDDYRRIKEEFGAETKPVIVGP
YTFVTLAKGYEESEAKAIQKRLVPLYVQLLKELEAEGVKWVQIDEPALVTASSEDVRAAKELYESITKELSGLNVLLQTY
FDSVDAYEELVSYPVQGIGLDFVHDKGRNLDQLKKHGFPKDKVLAAGVIDGRNIWKIDVEERLDAALDILSHADVEELWI
QPSSSLLHVPVAKHPDEHLEKDLLNGLSYAKEKLAELGVLKEGLLSGKAAVSEQIEESKAALKALKAFATGANSAQKEEL
NQLTEKDFSRPASFEERLALQNESLGLPLLPTTTIGSFPQSAEVRSARQKWRKKDWTDEQYQAFINAETKRWVDIQEEIG
LDVLVHGEFERTDMVEYFGEKLAGFAFTKYAWVQSYGSRCVRPPVIFGDVEFIEPMTVKDTVYAQSLTEKHMKGMLTGPV
TILNWSFPRVDISRKEIAFQIGLALRKEVKALEDAGIQIIQVDEPALREGLPLKTQDWDEYLTWAAEAFRLTTSSVQNET
QIHTHMCYSNFEDIVDTINDLDADVITIEHSRSHGGFLEYLKEHPYVKGLGLGVYDIHSPRVPATEEIYQIIDDALEVCP
TDRFWVNPDCGLKTRQQEETVAALKNMVDAAKQARKKQAQLV

Sequences:

>Translated_762_residues
MTTIKTSNLGFPRIGLNREWKKTLEAYWKGNTDKETFLKELDGLFLSAVKTQLDQHIDIVPVSDFTHYDHVLDTAVSFNW
IPKRFRSITDPVDTYFAIARGVKDAVSSEMTKWFNTNYHYIVPEYDESIEFRLTRNKQLDDYRRIKEEFGAETKPVIVGP
YTFVTLAKGYEESEAKAIQKRLVPLYVQLLKELEAEGVKWVQIDEPALVTASSEDVRAAKELYESITKELSGLNVLLQTY
FDSVDAYEELVSYPVQGIGLDFVHDKGRNLDQLKKHGFPKDKVLAAGVIDGRNIWKIDVEERLDAALDILSHADVEELWI
QPSSSLLHVPVAKHPDEHLEKDLLNGLSYAKEKLAELGVLKEGLLSGKAAVSEQIEESKAALKALKAFATGANSAQKEEL
NQLTEKDFSRPASFEERLALQNESLGLPLLPTTTIGSFPQSAEVRSARQKWRKKDWTDEQYQAFINAETKRWVDIQEEIG
LDVLVHGEFERTDMVEYFGEKLAGFAFTKYAWVQSYGSRCVRPPVIFGDVEFIEPMTVKDTVYAQSLTEKHMKGMLTGPV
TILNWSFPRVDISRKEIAFQIGLALRKEVKALEDAGIQIIQVDEPALREGLPLKTQDWDEYLTWAAEAFRLTTSSVQNET
QIHTHMCYSNFEDIVDTINDLDADVITIEHSRSHGGFLEYLKEHPYVKGLGLGVYDIHSPRVPATEEIYQIIDDALEVCP
TDRFWVNPDCGLKTRQQEETVAALKNMVDAAKQARKKQAQLV
>Mature_761_residues
TTIKTSNLGFPRIGLNREWKKTLEAYWKGNTDKETFLKELDGLFLSAVKTQLDQHIDIVPVSDFTHYDHVLDTAVSFNWI
PKRFRSITDPVDTYFAIARGVKDAVSSEMTKWFNTNYHYIVPEYDESIEFRLTRNKQLDDYRRIKEEFGAETKPVIVGPY
TFVTLAKGYEESEAKAIQKRLVPLYVQLLKELEAEGVKWVQIDEPALVTASSEDVRAAKELYESITKELSGLNVLLQTYF
DSVDAYEELVSYPVQGIGLDFVHDKGRNLDQLKKHGFPKDKVLAAGVIDGRNIWKIDVEERLDAALDILSHADVEELWIQ
PSSSLLHVPVAKHPDEHLEKDLLNGLSYAKEKLAELGVLKEGLLSGKAAVSEQIEESKAALKALKAFATGANSAQKEELN
QLTEKDFSRPASFEERLALQNESLGLPLLPTTTIGSFPQSAEVRSARQKWRKKDWTDEQYQAFINAETKRWVDIQEEIGL
DVLVHGEFERTDMVEYFGEKLAGFAFTKYAWVQSYGSRCVRPPVIFGDVEFIEPMTVKDTVYAQSLTEKHMKGMLTGPVT
ILNWSFPRVDISRKEIAFQIGLALRKEVKALEDAGIQIIQVDEPALREGLPLKTQDWDEYLTWAAEAFRLTTSSVQNETQ
IHTHMCYSNFEDIVDTINDLDADVITIEHSRSHGGFLEYLKEHPYVKGLGLGVYDIHSPRVPATEEIYQIIDDALEVCPT
DRFWVNPDCGLKTRQQEETVAALKNMVDAAKQARKKQAQLV

Specific function: Catalyzes the transfer of a methyl group from 5- methyltetrahydrofolate to homocysteine resulting in methionine formation

COG id: COG0620

COG function: function code E; Methionine synthase II (cobalamin-independent)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the vitamin-B12 independent methionine synthase family

Homologues:

Organism=Escherichia coli, GI2367304, Length=763, Percent_Identity=46.1336828309305, Blast_Score=637, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6320936, Length=775, Percent_Identity=42.1935483870968, Blast_Score=581, Evalue=1e-166,

Paralogues:

None

Copy number: 45,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): METE_BACA2 (A7Z3U1)

Other databases:

- EMBL:   CP000560
- RefSeq:   YP_001420898.1
- ProteinModelPortal:   A7Z3U1
- SMR:   A7Z3U1
- STRING:   A7Z3U1
- EnsemblBacteria:   EBBACT00000005136
- GeneID:   5461876
- GenomeReviews:   CP000560_GR
- KEGG:   bay:RBAM_013040
- eggNOG:   COG0620
- GeneTree:   EBGT00050000001686
- HOGENOM:   HBG287495
- OMA:   RFGWVQS
- ProtClustDB:   PRK05222
- BioCyc:   BAMY326423:RBAM_013040-MONOMER
- HAMAP:   MF_00172
- InterPro:   IPR013215
- InterPro:   IPR006276
- InterPro:   IPR002629
- PIRSF:   PIRSF000382
- TIGRFAMs:   TIGR01371

Pfam domain/function: PF08267 Meth_synt_1; PF01717 Meth_synt_2

EC number: =2.1.1.14

Molecular weight: Translated: 86582; Mature: 86451

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTIKTSNLGFPRIGLNREWKKTLEAYWKGNTDKETFLKELDGLFLSAVKTQLDQHIDIV
CCEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEE
PVSDFTHYDHVLDTAVSFNWIPKRFRSITDPVDTYFAIARGVKDAVSSEMTKWFNTNYHY
ECCCCHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
IVPEYDESIEFRLTRNKQLDDYRRIKEEFGAETKPVIVGPYTFVTLAKGYEESEAKAIQK
EECCCCCCEEEEEECCCCCHHHHHHHHHHCCCCCCEEECCHHHHHHHCCCCHHHHHHHHH
RLVPLYVQLLKELEAEGVKWVQIDEPALVTASSEDVRAAKELYESITKELSGLNVLLQTY
HHHHHHHHHHHHHHHCCCEEEEECCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
FDSVDAYEELVSYPVQGIGLDFVHDKGRNLDQLKKHGFPKDKVLAAGVIDGRNIWKIDVE
HHHHHHHHHHHHCCHHHCCEEEECCCCCCHHHHHHCCCCHHHEEEEEEECCCCEEEEEHH
ERLDAALDILSHADVEELWIQPSSSLLHVPVAKHPDEHLEKDLLNGLSYAKEKLAELGVL
HHHHHHHHHHHCCCHHHHHCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
KEGLLSGKAAVSEQIEESKAALKALKAFATGANSAQKEELNQLTEKDFSRPASFEERLAL
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHH
QNESLGLPLLPTTTIGSFPQSAEVRSARQKWRKKDWTDEQYQAFINAETKRWVDIQEEIG
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHCCCCHHHCCCHHHCC
LDVLVHGEFERTDMVEYFGEKLAGFAFTKYAWVQSYGSRCVRPPVIFGDVEFIEPMTVKD
CEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCHHHCCCCCHHH
TVYAQSLTEKHMKGMLTGPVTILNWSFPRVDISRKEIAFQIGLALRKEVKALEDAGIQII
HHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEE
QVDEPALREGLPLKTQDWDEYLTWAAEAFRLTTSSVQNETQIHTHMCYSNFEDIVDTIND
EECCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
LDADVITIEHSRSHGGFLEYLKEHPYVKGLGLGVYDIHSPRVPATEEIYQIIDDALEVCP
CCCCEEEEECCCCCCHHHHHHHHCCCEEECCCCEEECCCCCCCCHHHHHHHHHHHHHHCC
TDRFWVNPDCGLKTRQQEETVAALKNMVDAAKQARKKQAQLV
CCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TTIKTSNLGFPRIGLNREWKKTLEAYWKGNTDKETFLKELDGLFLSAVKTQLDQHIDIV
CEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEE
PVSDFTHYDHVLDTAVSFNWIPKRFRSITDPVDTYFAIARGVKDAVSSEMTKWFNTNYHY
ECCCCHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
IVPEYDESIEFRLTRNKQLDDYRRIKEEFGAETKPVIVGPYTFVTLAKGYEESEAKAIQK
EECCCCCCEEEEEECCCCCHHHHHHHHHHCCCCCCEEECCHHHHHHHCCCCHHHHHHHHH
RLVPLYVQLLKELEAEGVKWVQIDEPALVTASSEDVRAAKELYESITKELSGLNVLLQTY
HHHHHHHHHHHHHHHCCCEEEEECCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
FDSVDAYEELVSYPVQGIGLDFVHDKGRNLDQLKKHGFPKDKVLAAGVIDGRNIWKIDVE
HHHHHHHHHHHHCCHHHCCEEEECCCCCCHHHHHHCCCCHHHEEEEEEECCCCEEEEEHH
ERLDAALDILSHADVEELWIQPSSSLLHVPVAKHPDEHLEKDLLNGLSYAKEKLAELGVL
HHHHHHHHHHHCCCHHHHHCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
KEGLLSGKAAVSEQIEESKAALKALKAFATGANSAQKEELNQLTEKDFSRPASFEERLAL
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHH
QNESLGLPLLPTTTIGSFPQSAEVRSARQKWRKKDWTDEQYQAFINAETKRWVDIQEEIG
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHCCCCHHHCCCHHHCC
LDVLVHGEFERTDMVEYFGEKLAGFAFTKYAWVQSYGSRCVRPPVIFGDVEFIEPMTVKD
CEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCHHHCCCCCHHH
TVYAQSLTEKHMKGMLTGPVTILNWSFPRVDISRKEIAFQIGLALRKEVKALEDAGIQII
HHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEE
QVDEPALREGLPLKTQDWDEYLTWAAEAFRLTTSSVQNETQIHTHMCYSNFEDIVDTIND
EECCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
LDADVITIEHSRSHGGFLEYLKEHPYVKGLGLGVYDIHSPRVPATEEIYQIIDDALEVCP
CCCCEEEEECCCCCCHHHHHHHHCCCEEECCCCEEECCCCCCCCHHHHHHHHHHHHHHCC
TDRFWVNPDCGLKTRQQEETVAALKNMVDAAKQARKKQAQLV
CCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA