Click here to switch to the map view.

The map label for this gene is ntrX [H]

Identifier: 154248317

GI number: 154248317

Start: 4874555

End: 4875922

Strand: Reverse

Name: ntrX [H]

Synonym: Xaut_4397

Alternate gene names: 154248317

Gene position: 4875922-4874555 (Counterclockwise)

Preceding gene: 154248318

Following gene: 154248316

Centisome position: 91.84

GC content: 67.84

Gene sequence:

>1368_bases
ATGGCCATGGACATTCTCATCGTGGACGATGAGGCCGACATCTGCGAACTGGTGGCCGGCATCCTGGAGGACGAGGGCTA
TGCCGCCCGCACCGCCCGGGACGCCGACAGCGCCCTCGCGGCCATCTCGGCGCGGCGGCCTAACCTGGTCTTCCTGGACA
TCTGGCTGCAGGGCTCGCGGCTCGACGGGCTGGAACTGCTGAACGAGATCAAGGCCCAGCACCCAGAGGTGCCGGTGGTG
ATGATCTCCGGCCACGGCAACATCGAGACCGCTGTGGCGGCCATCAAGCGCGGCGCCTACGACTTCATCGAGAAGCCGTT
CAATGCCGACCGCCTGGTGATGGTGGCCGAGCGCGCGCTGGAGACGCTGCGCCTGAGGCGCGAGCTGAAGGAGCTGAAGC
AGCTGGCGCCCCACGGCCATGTGCTGGTGGGCCGCTCCAGCGCCGTCCAGCAGCTGAAGGCCACCATCGACCGGGTCGGG
CCCACCAACAGCCGCATCCTCATCGTCGGCCCTTCCGGCTCCGGCAAGGAGCTGACGGCCCGGCTGATCCACACCACCTC
GACCCGCGCCCAGGGGCCGTTCGTGGTGCTGAACTCTGCGGCCATCACCCCGGAGCGGCTGGAATTCGAGCTGTTCGGCG
TGGCGGAAGGGGAGGGGCGAGAGCCCAAGCGCGGGGCGCTGGACGAGGCCCACGGTGGCACGCTGTTCCTGGACGAAATC
TCCGACATGCCGCGCGAGACCCAGAACCGGGTGCTGCGCGTTCTGGTGGAGCAGACCTTCTCGCGTATCGGCTCCAACGA
CAAGGTCAGCGTGGACGTGCGCATCATCTCCTCCACCGGGCGCAACCTGGAGGAGGAGATCGCCAAGGGCCGATTCCGCG
AGGATCTCTACCACCGCCTCTCAGTGGTGCCGATCCGGGTGCCGCCGCTGTCCGAGCGGCGCGACGACATTCCGGACCTG
GTGGAATTCTTCGTCGACACCATCTCGCAGACCACCGGCCTGCCGCGCCGGCCCATCGGCGAGGATGCCATGGCGGTGCT
CCAGTCCCACGACTGGCCGGGCAACGTGCGCCAGCTACGCAACAACGTGGAACGGCTGCTGATTCTCGCCGGTGGCGACC
TGGAAGCCACCATCACCGCCGCCATGCTGCCGCCGGACGTGGGCGCGCTGGTGCCCAACCTGCCCAACGGCAATGGCGGC
GAGCATCTCATGGGCCTGCCGTTGCGTGAGGCACGCGAGGTGTTCGAGCGGGAATACCTTGCCGCCCAGATCAACCGCTT
CGGCGGCAACATCTCGCGGACGGCGGAATTCGTCGGCATGGAGCGCTCGGCCCTTCATCGAAAGCTTAAGGCTCTCGGGG
TAGGCTGA

Upstream 100 bases:

>100_bases
CCGGGGGGCCTGGATCCGGCTTCGGATGCGGGCAGACGGTCAACCACCGGCCGGCGCGGGCGGGGCGCAACCGGTCCCTG
AAACAGGGAGGGCCTGACAC

Downstream 100 bases:

>100_bases
CGGACCAGTTGACGGGATACCCGAAAAAGGGGCTGCATCTGAGCTGAGGCTCAAGGTGCGATCGGTTTTGCCGCGATCTT
TCAGGTCGCGATTCGCCTGG

Product: two component sigma54 specific Fis family transcriptional regulator

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 455; Mature: 454

Protein sequence:

>455_residues
MAMDILIVDDEADICELVAGILEDEGYAARTARDADSALAAISARRPNLVFLDIWLQGSRLDGLELLNEIKAQHPEVPVV
MISGHGNIETAVAAIKRGAYDFIEKPFNADRLVMVAERALETLRLRRELKELKQLAPHGHVLVGRSSAVQQLKATIDRVG
PTNSRILIVGPSGSGKELTARLIHTTSTRAQGPFVVLNSAAITPERLEFELFGVAEGEGREPKRGALDEAHGGTLFLDEI
SDMPRETQNRVLRVLVEQTFSRIGSNDKVSVDVRIISSTGRNLEEEIAKGRFREDLYHRLSVVPIRVPPLSERRDDIPDL
VEFFVDTISQTTGLPRRPIGEDAMAVLQSHDWPGNVRQLRNNVERLLILAGGDLEATITAAMLPPDVGALVPNLPNGNGG
EHLMGLPLREAREVFEREYLAAQINRFGGNISRTAEFVGMERSALHRKLKALGVG

Sequences:

>Translated_455_residues
MAMDILIVDDEADICELVAGILEDEGYAARTARDADSALAAISARRPNLVFLDIWLQGSRLDGLELLNEIKAQHPEVPVV
MISGHGNIETAVAAIKRGAYDFIEKPFNADRLVMVAERALETLRLRRELKELKQLAPHGHVLVGRSSAVQQLKATIDRVG
PTNSRILIVGPSGSGKELTARLIHTTSTRAQGPFVVLNSAAITPERLEFELFGVAEGEGREPKRGALDEAHGGTLFLDEI
SDMPRETQNRVLRVLVEQTFSRIGSNDKVSVDVRIISSTGRNLEEEIAKGRFREDLYHRLSVVPIRVPPLSERRDDIPDL
VEFFVDTISQTTGLPRRPIGEDAMAVLQSHDWPGNVRQLRNNVERLLILAGGDLEATITAAMLPPDVGALVPNLPNGNGG
EHLMGLPLREAREVFEREYLAAQINRFGGNISRTAEFVGMERSALHRKLKALGVG
>Mature_454_residues
AMDILIVDDEADICELVAGILEDEGYAARTARDADSALAAISARRPNLVFLDIWLQGSRLDGLELLNEIKAQHPEVPVVM
ISGHGNIETAVAAIKRGAYDFIEKPFNADRLVMVAERALETLRLRRELKELKQLAPHGHVLVGRSSAVQQLKATIDRVGP
TNSRILIVGPSGSGKELTARLIHTTSTRAQGPFVVLNSAAITPERLEFELFGVAEGEGREPKRGALDEAHGGTLFLDEIS
DMPRETQNRVLRVLVEQTFSRIGSNDKVSVDVRIISSTGRNLEEEIAKGRFREDLYHRLSVVPIRVPPLSERRDDIPDLV
EFFVDTISQTTGLPRRPIGEDAMAVLQSHDWPGNVRQLRNNVERLLILAGGDLEATITAAMLPPDVGALVPNLPNGNGGE
HLMGLPLREAREVFEREYLAAQINRFGGNISRTAEFVGMERSALHRKLKALGVG

Specific function: Member of the two-component regulatory system ntrY/ntrX involved in the activation of nitrogen assimilatory genes such as glnA. NtrX is probably phosphorylated by ntrY and interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=470, Percent_Identity=35.531914893617, Blast_Score=289, Evalue=3e-79,
Organism=Escherichia coli, GI1790437, Length=450, Percent_Identity=38.2222222222222, Blast_Score=276, Evalue=2e-75,
Organism=Escherichia coli, GI1788550, Length=462, Percent_Identity=34.4155844155844, Blast_Score=274, Evalue=7e-75,
Organism=Escherichia coli, GI1788905, Length=454, Percent_Identity=35.2422907488987, Blast_Score=238, Evalue=4e-64,
Organism=Escherichia coli, GI1789233, Length=333, Percent_Identity=37.5375375375375, Blast_Score=204, Evalue=1e-53,
Organism=Escherichia coli, GI87082152, Length=327, Percent_Identity=38.2262996941896, Blast_Score=189, Evalue=4e-49,
Organism=Escherichia coli, GI1786524, Length=328, Percent_Identity=36.280487804878, Blast_Score=186, Evalue=2e-48,
Organism=Escherichia coli, GI1787583, Length=336, Percent_Identity=35.7142857142857, Blast_Score=182, Evalue=3e-47,
Organism=Escherichia coli, GI1789087, Length=247, Percent_Identity=41.2955465587045, Blast_Score=181, Evalue=7e-47,
Organism=Escherichia coli, GI87082117, Length=235, Percent_Identity=40.8510638297872, Blast_Score=179, Evalue=5e-46,
Organism=Escherichia coli, GI87081872, Length=333, Percent_Identity=32.7327327327327, Blast_Score=160, Evalue=2e-40,
Organism=Escherichia coli, GI87081858, Length=303, Percent_Identity=30.03300330033, Blast_Score=119, Evalue=4e-28,
Organism=Escherichia coli, GI1789828, Length=216, Percent_Identity=35.1851851851852, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1786599, Length=115, Percent_Identity=37.3913043478261, Blast_Score=75, Evalue=9e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 49979; Mature: 49848

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAMDILIVDDEADICELVAGILEDEGYAARTARDADSALAAISARRPNLVFLDIWLQGSR
CCEEEEEECCCCHHHHHHHHHHCCCCCHHHCCCCHHHHHHHHHCCCCCEEEEEEEEECCC
LDGLELLNEIKAQHPEVPVVMISGHGNIETAVAAIKRGAYDFIEKPFNADRLVMVAERAL
CCHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHCCHHHHHCCCCCCHHHHHHHHHH
ETLRLRRELKELKQLAPHGHVLVGRSSAVQQLKATIDRVGPTNSRILIVGPSGSGKELTA
HHHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHH
RLIHTTSTRAQGPFVVLNSAAITPERLEFELFGVAEGEGREPKRGALDEAHGGTLFLDEI
HHHEECCCCCCCCEEEEECCCCCHHHEEEEEEEEECCCCCCCCCCCCCCCCCCEEEHHHH
SDMPRETQNRVLRVLVEQTFSRIGSNDKVSVDVRIISSTGRNLEEEIAKGRFREDLYHRL
HCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCHHHHHHHCHHHHHHHHHH
SVVPIRVPPLSERRDDIPDLVEFFVDTISQTTGLPRRPIGEDAMAVLQSHDWPGNVRQLR
EEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCHHHHHH
NNVERLLILAGGDLEATITAAMLPPDVGALVPNLPNGNGGEHLMGLPLREAREVFEREYL
HHHHEEEEEECCCCCEEEEEEECCCCHHHHCCCCCCCCCCCCEECCCHHHHHHHHHHHHH
AAQINRFGGNISRTAEFVGMERSALHRKLKALGVG
HHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AMDILIVDDEADICELVAGILEDEGYAARTARDADSALAAISARRPNLVFLDIWLQGSR
CEEEEEECCCCHHHHHHHHHHCCCCCHHHCCCCHHHHHHHHHCCCCCEEEEEEEEECCC
LDGLELLNEIKAQHPEVPVVMISGHGNIETAVAAIKRGAYDFIEKPFNADRLVMVAERAL
CCHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHCCHHHHHCCCCCCHHHHHHHHHH
ETLRLRRELKELKQLAPHGHVLVGRSSAVQQLKATIDRVGPTNSRILIVGPSGSGKELTA
HHHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHH
RLIHTTSTRAQGPFVVLNSAAITPERLEFELFGVAEGEGREPKRGALDEAHGGTLFLDEI
HHHEECCCCCCCCEEEEECCCCCHHHEEEEEEEEECCCCCCCCCCCCCCCCCCEEEHHHH
SDMPRETQNRVLRVLVEQTFSRIGSNDKVSVDVRIISSTGRNLEEEIAKGRFREDLYHRL
HCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCHHHHHHHCHHHHHHHHHH
SVVPIRVPPLSERRDDIPDLVEFFVDTISQTTGLPRRPIGEDAMAVLQSHDWPGNVRQLR
EEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCHHHHHH
NNVERLLILAGGDLEATITAAMLPPDVGALVPNLPNGNGGEHLMGLPLREAREVFEREYL
HHHHEEEEEECCCCCEEEEEEECCCCHHHHCCCCCCCCCCCCEECCCHHHHHHHHHHHHH
AAQINRFGGNISRTAEFVGMERSALHRKLKALGVG
HHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1661370 [H]