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The map label for this gene is pyrB

Identifier: 154247842

GI number: 154247842

Start: 4338079

End: 4339026

Strand: Reverse

Name: pyrB

Synonym: Xaut_3919

Alternate gene names: 154247842

Gene position: 4339026-4338079 (Counterclockwise)

Preceding gene: 154247844

Following gene: 154247841

Centisome position: 81.73

GC content: 66.98

Gene sequence:

>948_bases
ATGCATGCTGCTTCCGCCTTCACCCTCTCCGGCCGGGATCTTCTCGGCATCGAAGGCCTCATCGCCAGCGAGATCACAGG
TCTGCTCGACCTTGCCGAGGAGTTCGTCGAACTCAACCGGCAGATCGAGAAGAAGCGCACCACGCTTCGCGGCCGCACCC
AGATCAACCTGTTCTCCGAGGCCTCCACCCGCACCCAGTCCTCCTTCGAGATCGCCGGAAAGCGGCTCGGGGCGGATGTG
ATGAACATGTCGGTGGGCTCGTCCTCCGTGAAGAAGGGCGAGACGCTGATCGACACCGCCATCACGCTGAATGCCATGCA
CCCCGACATCTTGGTGGTGCGCCATCACGCGTCCGGCGCGGTGGCGCTTCTGGCGCGCAAGGTGGACTGCTGCGTGGTGA
ATGCCGGCGACGGCGCCCACGAGCACCCCACCCAGGCCCTGCTGGACGCCCTCACCATCCGCCGCAACAAGGGCCGCATC
GCCGGCCTCACCGTCGCCATCTGCGGCGACGTGCTGCATTCGCGGGTGGCGCGCTCCAACATCATCCTCCTCCACACCCT
CGGCGCGAAGGTGCGGCTGGTGGCGCCCTCCACCCTGCTGCCGGCGGGCATCGAGAGCCTCGGCGTCGAGGTGTTCCGCA
CCATGGAAGAGGGCCTCAAGGACGCGGACATCGTGATGATGCTGCGCCTCCAGCGCGAGCGCATGGCCGGCTCCTTCATC
CCTTCGGTGAAGGAGTATTTCCATTATTTCGGCCTCGACGAGGCCAAGCTGCGCCATGCCGCCCCCGACGCGCTGGTGAT
GCATCCCGGCCCCATGAACCGGGGCGTGGAGATCGATTCGGCGGTGGCCGACGGGCCTCGCTCCCTCATCCGCGAACAGG
TGGAGATGGGCGTCGCCGTGCGCATGGCCGTGCTCGACACCCTCGCCCGGAAGCTGCCCAATGCGTGA

Upstream 100 bases:

>100_bases
GTCGGACCGGCGATCCGAAAAGCGACCGGCTCCTGCCGCTCGTCCCTTTGACCGCCTCCCTTGCCGGGCGGGCCTCGTGC
GTCTATGCCTGCCGCTTGCC

Downstream 100 bases:

>100_bases
CGGCCGCCTCATCCTCCGCGATCCCCACCCGCTCTTGCTCGCCAACGCGCGGGTCATCGATCCCTCCCGTGGCTCGGATT
TCCACGGCGATGTCTATCTC

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase

Number of amino acids: Translated: 315; Mature: 315

Protein sequence:

>315_residues
MHAASAFTLSGRDLLGIEGLIASEITGLLDLAEEFVELNRQIEKKRTTLRGRTQINLFSEASTRTQSSFEIAGKRLGADV
MNMSVGSSSVKKGETLIDTAITLNAMHPDILVVRHHASGAVALLARKVDCCVVNAGDGAHEHPTQALLDALTIRRNKGRI
AGLTVAICGDVLHSRVARSNIILLHTLGAKVRLVAPSTLLPAGIESLGVEVFRTMEEGLKDADIVMMLRLQRERMAGSFI
PSVKEYFHYFGLDEAKLRHAAPDALVMHPGPMNRGVEIDSAVADGPRSLIREQVEMGVAVRMAVLDTLARKLPNA

Sequences:

>Translated_315_residues
MHAASAFTLSGRDLLGIEGLIASEITGLLDLAEEFVELNRQIEKKRTTLRGRTQINLFSEASTRTQSSFEIAGKRLGADV
MNMSVGSSSVKKGETLIDTAITLNAMHPDILVVRHHASGAVALLARKVDCCVVNAGDGAHEHPTQALLDALTIRRNKGRI
AGLTVAICGDVLHSRVARSNIILLHTLGAKVRLVAPSTLLPAGIESLGVEVFRTMEEGLKDADIVMMLRLQRERMAGSFI
PSVKEYFHYFGLDEAKLRHAAPDALVMHPGPMNRGVEIDSAVADGPRSLIREQVEMGVAVRMAVLDTLARKLPNA
>Mature_315_residues
MHAASAFTLSGRDLLGIEGLIASEITGLLDLAEEFVELNRQIEKKRTTLRGRTQINLFSEASTRTQSSFEIAGKRLGADV
MNMSVGSSSVKKGETLIDTAITLNAMHPDILVVRHHASGAVALLARKVDCCVVNAGDGAHEHPTQALLDALTIRRNKGRI
AGLTVAICGDVLHSRVARSNIILLHTLGAKVRLVAPSTLLPAGIESLGVEVFRTMEEGLKDADIVMMLRLQRERMAGSFI
PSVKEYFHYFGLDEAKLRHAAPDALVMHPGPMNRGVEIDSAVADGPRSLIREQVEMGVAVRMAVLDTLARKLPNA

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family

Homologues:

Organism=Homo sapiens, GI18105007, Length=313, Percent_Identity=37.6996805111821, Blast_Score=179, Evalue=2e-45,
Organism=Homo sapiens, GI38788445, Length=312, Percent_Identity=24.6794871794872, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI2367364, Length=269, Percent_Identity=36.0594795539033, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI1790703, Length=192, Percent_Identity=29.1666666666667, Blast_Score=62, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI193204318, Length=341, Percent_Identity=35.4838709677419, Blast_Score=138, Evalue=4e-33,
Organism=Saccharomyces cerevisiae, GI6322331, Length=307, Percent_Identity=35.5048859934853, Blast_Score=143, Evalue=3e-35,
Organism=Drosophila melanogaster, GI24642586, Length=310, Percent_Identity=34.8387096774194, Blast_Score=149, Evalue=3e-36,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): PYRB_XANP2 (A7IMA0)

Other databases:

- EMBL:   CP000781
- RefSeq:   YP_001418800.1
- ProteinModelPortal:   A7IMA0
- SMR:   A7IMA0
- STRING:   A7IMA0
- GeneID:   5420970
- GenomeReviews:   CP000781_GR
- KEGG:   xau:Xaut_3919
- eggNOG:   COG0540
- HOGENOM:   HBG579429
- OMA:   MNRGLEI
- ProtClustDB:   PRK00856
- BioCyc:   XAUT78245:XAUT_3919-MONOMER
- HAMAP:   MF_00001
- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082
- PRINTS:   PR00100
- PRINTS:   PR00101
- TIGRFAMs:   TIGR00670

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N; SSF53671 Asp/Orn_carbamoyltranf

EC number: =2.1.3.2

Molecular weight: Translated: 34021; Mature: 34021

Theoretical pI: Translated: 8.15; Mature: 8.15

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHAASAFTLSGRDLLGIEGLIASEITGLLDLAEEFVELNRQIEKKRTTLRGRTQINLFSE
CCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECC
ASTRTQSSFEIAGKRLGADVMNMSVGSSSVKKGETLIDTAITLNAMHPDILVVRHHASGA
CCCCCCHHHHHHHHHHCHHHHHCCCCCCHHHCCCHHHHHHEEEECCCCCEEEEEECCCCH
VALLARKVDCCVVNAGDGAHEHPTQALLDALTIRRNKGRIAGLTVAICGDVLHSRVARSN
HHHHHHHCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHCC
IILLHTLGAKVRLVAPSTLLPAGIESLGVEVFRTMEEGLKDADIVMMLRLQRERMAGSFI
EEEEEECCCEEEEECCCHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCC
PSVKEYFHYFGLDEAKLRHAAPDALVMHPGPMNRGVEIDSAVADGPRSLIREQVEMGVAV
HHHHHHHHHHCCCHHHHHHCCCCEEEECCCCCCCCCEECHHHCCCHHHHHHHHHHHHHHH
RMAVLDTLARKLPNA
HHHHHHHHHHHCCCC
>Mature Secondary Structure
MHAASAFTLSGRDLLGIEGLIASEITGLLDLAEEFVELNRQIEKKRTTLRGRTQINLFSE
CCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECC
ASTRTQSSFEIAGKRLGADVMNMSVGSSSVKKGETLIDTAITLNAMHPDILVVRHHASGA
CCCCCCHHHHHHHHHHCHHHHHCCCCCCHHHCCCHHHHHHEEEECCCCCEEEEEECCCCH
VALLARKVDCCVVNAGDGAHEHPTQALLDALTIRRNKGRIAGLTVAICGDVLHSRVARSN
HHHHHHHCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHCC
IILLHTLGAKVRLVAPSTLLPAGIESLGVEVFRTMEEGLKDADIVMMLRLQRERMAGSFI
EEEEEECCCEEEEECCCHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCC
PSVKEYFHYFGLDEAKLRHAAPDALVMHPGPMNRGVEIDSAVADGPRSLIREQVEMGVAV
HHHHHHHHHHCCCHHHHHHCCCCEEEECCCCCCCCCEECHHHCCCHHHHHHHHHHHHHHH
RMAVLDTLARKLPNA
HHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA