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The map label for this gene is apbE [H]

Identifier: 154247322

GI number: 154247322

Start: 3781306

End: 3782253

Strand: Reverse

Name: apbE [H]

Synonym: Xaut_3394

Alternate gene names: 154247322

Gene position: 3782253-3781306 (Counterclockwise)

Preceding gene: 154247323

Following gene: 154247321

Centisome position: 71.24

GC content: 72.05

Gene sequence:

>948_bases
ATGTCTATTGAGCCGGTCTCCCGACCCTCGGCGCTGAGCCGCCACGCGCTGAGCGGCCCCGCCATGGGGTCGCGCTGGTC
CGCTGTCTTCTGGTCGAACGCAAAGGTCGATATCGACGCGCTGACCGGCCGTCTCCAGGCGGCTGTCGATCGCGTGGAGC
AGCAGATGTCGGCTTGGCGGCGCGATTCCGACCTCGAACGCCTCAACGCCACGCCAGTCGGCGCGTGGACGGCGATCCCG
CGAGAGCTGTCGAAGGTGCTCACCACCGCGCTCGACATCGGCCGCCGCTCGGAGGGCGCGTTCGACATCGGCGTCGGCGA
CCTTGTCCGGGCCTGGGGCTGCGGCGGTGGATCCCGCACGCCCGATGCGGAACTGATCGCCGGGCTCGCCGGACATCCCT
CGTTCGAGCCGCCGAAGACGCTGCAACTCGACATGGCCGGCGGCCGGGCCCGGCGCCTCGCGCCGCTGCGGCTGGACCTG
TCCGGCATCGCCAAGGGCTTCGGCGTGGACGAACTGGCGCGGGAGCTGGGCGCGACCGGCTGCTCCTGTTGGCTGGTGGG
CATCGACGGCGAGATGCGGGCCGAGGGCGTGAAGCCGGATGGACGCCCCTGGGCCGTCGCCCACGAGAAGCCGGTTCCCG
GCGCGCGGGAGGTCCTCGGCATGCTGGAGCTCTCGGGCGCCGCCGTCGCCACCTCCGGCTCCTATCGCCATCGTGTCGAG
GTTGACGGGCGAACCACCTCTCACACCATGGACCCACGCCGCGGCGCGCCGCTGGACAATGATCTGTCCGCCGTCACCGT
GCTTGCCGAAACCTGCATGGTGGCGGACGCCTGGGCCACCGCGCTGCTGGTGGCCGGCGCCGTGCGCGGACCCGAGCTCG
CCCGCAGGCAGGGCCTCGCCGCCCTGTTCGTGCGAACCGATGGCACGGTGCTGGAAGCCTGGCCTTGA

Upstream 100 bases:

>100_bases
CGCGACATGGCCGAGGCGGTGACGCGTGCCTTCGAGCCGGTGGTGCGACCGCTGGGCCTCGATCTTGCGACCCTCAAATC
TCACGGACGCTACGTCGAAG

Downstream 100 bases:

>100_bases
GCTGCTTGACCTTGAGCTGCTTGACCTTGAGCCGCTTGACCTTGTGCCGCCTTAACCACAAGCGCAGCCCATCGACCTGT
TTCATGCCACGGCGGGCGTC

Product: ApbE family lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 315; Mature: 314

Protein sequence:

>315_residues
MSIEPVSRPSALSRHALSGPAMGSRWSAVFWSNAKVDIDALTGRLQAAVDRVEQQMSAWRRDSDLERLNATPVGAWTAIP
RELSKVLTTALDIGRRSEGAFDIGVGDLVRAWGCGGGSRTPDAELIAGLAGHPSFEPPKTLQLDMAGGRARRLAPLRLDL
SGIAKGFGVDELARELGATGCSCWLVGIDGEMRAEGVKPDGRPWAVAHEKPVPGAREVLGMLELSGAAVATSGSYRHRVE
VDGRTTSHTMDPRRGAPLDNDLSAVTVLAETCMVADAWATALLVAGAVRGPELARRQGLAALFVRTDGTVLEAWP

Sequences:

>Translated_315_residues
MSIEPVSRPSALSRHALSGPAMGSRWSAVFWSNAKVDIDALTGRLQAAVDRVEQQMSAWRRDSDLERLNATPVGAWTAIP
RELSKVLTTALDIGRRSEGAFDIGVGDLVRAWGCGGGSRTPDAELIAGLAGHPSFEPPKTLQLDMAGGRARRLAPLRLDL
SGIAKGFGVDELARELGATGCSCWLVGIDGEMRAEGVKPDGRPWAVAHEKPVPGAREVLGMLELSGAAVATSGSYRHRVE
VDGRTTSHTMDPRRGAPLDNDLSAVTVLAETCMVADAWATALLVAGAVRGPELARRQGLAALFVRTDGTVLEAWP
>Mature_314_residues
SIEPVSRPSALSRHALSGPAMGSRWSAVFWSNAKVDIDALTGRLQAAVDRVEQQMSAWRRDSDLERLNATPVGAWTAIPR
ELSKVLTTALDIGRRSEGAFDIGVGDLVRAWGCGGGSRTPDAELIAGLAGHPSFEPPKTLQLDMAGGRARRLAPLRLDLS
GIAKGFGVDELARELGATGCSCWLVGIDGEMRAEGVKPDGRPWAVAHEKPVPGAREVLGMLELSGAAVATSGSYRHRVEV
DGRTTSHTMDPRRGAPLDNDLSAVTVLAETCMVADAWATALLVAGAVRGPELARRQGLAALFVRTDGTVLEAWP

Specific function: Involved in the conversion of aminoimidazole ribotide (AIR), a purine intermediate, to the 4-amino-5-hydroxymethyl-2- methyl pyrimidine (HMP) moiety of thiamine [H]

COG id: COG1477

COG function: function code H; Membrane-associated lipoprotein involved in thiamine biosynthesis

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ApbE family [H]

Homologues:

Organism=Escherichia coli, GI1788543, Length=316, Percent_Identity=31.3291139240506, Blast_Score=181, Evalue=5e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003374 [H]

Pfam domain/function: PF02424 ApbE [H]

EC number: NA

Molecular weight: Translated: 33342; Mature: 33211

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIEPVSRPSALSRHALSGPAMGSRWSAVFWSNAKVDIDALTGRLQAAVDRVEQQMSAWR
CCCCCCCCCHHHHHHHCCCCCCCCCEEEEEECCCEEEHHHHHHHHHHHHHHHHHHHHHHH
RDSDLERLNATPVGAWTAIPRELSKVLTTALDIGRRSEGAFDIGVGDLVRAWGCGGGSRT
CCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEECCHHHHHHHHCCCCCCCC
PDAELIAGLAGHPSFEPPKTLQLDMAGGRARRLAPLRLDLSGIAKGFGVDELARELGATG
CHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCEEEECHHHHHCCCCHHHHHHHHCCCC
CSCWLVGIDGEMRAEGVKPDGRPWAVAHEKPVPGAREVLGMLELSGAAVATSGSYRHRVE
CEEEEEECCCCHHCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHCCCEEEECCCCEEEEE
VDGRTTSHTMDPRRGAPLDNDLSAVTVLAETCMVADAWATALLVAGAVRGPELARRQGLA
ECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCE
ALFVRTDGTVLEAWP
EEEEECCCCEEECCC
>Mature Secondary Structure 
SIEPVSRPSALSRHALSGPAMGSRWSAVFWSNAKVDIDALTGRLQAAVDRVEQQMSAWR
CCCCCCCCHHHHHHHCCCCCCCCCEEEEEECCCEEEHHHHHHHHHHHHHHHHHHHHHHH
RDSDLERLNATPVGAWTAIPRELSKVLTTALDIGRRSEGAFDIGVGDLVRAWGCGGGSRT
CCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEECCHHHHHHHHCCCCCCCC
PDAELIAGLAGHPSFEPPKTLQLDMAGGRARRLAPLRLDLSGIAKGFGVDELARELGATG
CHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCEEEECHHHHHCCCCHHHHHHHHCCCC
CSCWLVGIDGEMRAEGVKPDGRPWAVAHEKPVPGAREVLGMLELSGAAVATSGSYRHRVE
CEEEEEECCCCHHCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHCCCEEEECCCCEEEEE
VDGRTTSHTMDPRRGAPLDNDLSAVTVLAETCMVADAWATALLVAGAVRGPELARRQGLA
ECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCE
ALFVRTDGTVLEAWP
EEEEECCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]