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The map label for this gene is mltB [H]

Identifier: 154247321

GI number: 154247321

Start: 3779842

End: 3781071

Strand: Reverse

Name: mltB [H]

Synonym: Xaut_3393

Alternate gene names: 154247321

Gene position: 3781071-3779842 (Counterclockwise)

Preceding gene: 154247322

Following gene: 154247320

Centisome position: 71.22

GC content: 68.7

Gene sequence:

>1230_bases
TTGCCATCAGCTTGCCGCACCCTCCTCCTCGCCCTGCTGCTGTCGGCGCTGACGCTTCAAGCTTCCGCCCACGCGGCTGA
CGCCGCCTTCACTCAGTTCATCGCCTCTCTCTGGCCGCAGGCCCAGGCGGCCGGGGTTTCCCGCGCGACGTTCGAGAGGG
AAACGCGCGGCCTGGAGCCGGACTATAAGTTGCCCGATCTCGCCCTGCCGGGGCGGCCCGCGACAGGCGCGTCGGCGCAG
GCCGAGTTCGTGCAGGTTCCCGCGAGCTATGTGAAGGAGGCCACCATCGCCCGGCTTGCCGCCGACGGGCAGCGGCTGAT
GCAGAAGTATCGCGCCGCCCTCGACCAGATCGAAAGCCGGTTCGGCGTGCCGGGGACCATGGTGCTGGCGCTGTGGGGCC
GCGAGACCGATTACGGCCGATACACCTCCCCCTATGACACCCTGCGCGTGGTGGCGACACAGGCCTATGCGGGGCGCCGC
AAGGACCAGTATCGCGACGAGTTCATCCTCGCGCTGAAAATCCTCGATGAGGGCGCGGTGGCGCGCAAGGACTTCCGCTC
CTCCTGGGCCGGCGCCACCGGCCTCACGCAATTCCTGCCATCCGAATACTTCAAGCACGGCGTCGATCTCGACGGCGATG
GCCGCGTGGATATCTGGCATTCGGTGCCCGATGCGCTGGCCTCCGCCGCCAAGCAGCTGGCCGACAAGGGCTGGCAGCCC
GGCGTGCGCTGGGCCTATGAGGTCCGCGCGCCGGCCAACGCTGACTGCACCCTGGGCGTGCCGGAGGTGAAGAAGCCCAT
CGGGGAGTGGCTGCGCACCGGGTTCGCGCCGTTCGCGCGGATCTCGGCCGGCGAGCAGGCGCAGCCGGCGTCGTTGCTCC
AGCCCGAGGGCATCTACGGCCCCTCGTTCCTCACCACGGCCAACTATTTCGTGATCAAGCAGTACAATTTCTCCGATCTC
TACGTGCTGTTCGTCGGTCATCTCTCCGACCGCATCGCCGGCGCAGGGCCCTTCGCCACGCCGTGGGCGGCTTCGACCCA
GCTGCGCACGACCTCGGTCGCGGCGATGCAGAAACATCTGACGCGCGTCGGCCTCTACGCCGACAAGATCGACGGCAAGG
CGGGGATGCTGACCCGCGCCGCCCTCGGCGCCTACCAGAAATCGGCCGGGCTGAAGGTGGATTGCTGGCCGAGCGAGGCG
GTGCTGCAATCCATGGGCGCGGCGCGGTAG

Upstream 100 bases:

>100_bases
GCTTTGCCGTCGACGAGCCTTTCCGCCCGCGTTCACGGCAGCGCCGCCCCGTGCTAGCGTCGCGGATGCCCCATTTCGAC
ATCAAAGCGTGAGCGGTTCC

Downstream 100 bases:

>100_bases
CGGCCCGAGCAGGCCCGCTGCAACCGGTCACAAGGTCTCCGCTCCAATCTGGCGCGCAATATGCCTTGTGCGGCCCGGAC
CCGGCTTTTGGTCGGGTTTG

Product: lytic murein transglycosylase

Products: Muramic Acid Residue [C]

Alternate protein names: 35 kDa soluble lytic transglycosylase; Murein hydrolase B; Slt35 [H]

Number of amino acids: Translated: 409; Mature: 408

Protein sequence:

>409_residues
MPSACRTLLLALLLSALTLQASAHAADAAFTQFIASLWPQAQAAGVSRATFERETRGLEPDYKLPDLALPGRPATGASAQ
AEFVQVPASYVKEATIARLAADGQRLMQKYRAALDQIESRFGVPGTMVLALWGRETDYGRYTSPYDTLRVVATQAYAGRR
KDQYRDEFILALKILDEGAVARKDFRSSWAGATGLTQFLPSEYFKHGVDLDGDGRVDIWHSVPDALASAAKQLADKGWQP
GVRWAYEVRAPANADCTLGVPEVKKPIGEWLRTGFAPFARISAGEQAQPASLLQPEGIYGPSFLTTANYFVIKQYNFSDL
YVLFVGHLSDRIAGAGPFATPWAASTQLRTTSVAAMQKHLTRVGLYADKIDGKAGMLTRAALGAYQKSAGLKVDCWPSEA
VLQSMGAAR

Sequences:

>Translated_409_residues
MPSACRTLLLALLLSALTLQASAHAADAAFTQFIASLWPQAQAAGVSRATFERETRGLEPDYKLPDLALPGRPATGASAQ
AEFVQVPASYVKEATIARLAADGQRLMQKYRAALDQIESRFGVPGTMVLALWGRETDYGRYTSPYDTLRVVATQAYAGRR
KDQYRDEFILALKILDEGAVARKDFRSSWAGATGLTQFLPSEYFKHGVDLDGDGRVDIWHSVPDALASAAKQLADKGWQP
GVRWAYEVRAPANADCTLGVPEVKKPIGEWLRTGFAPFARISAGEQAQPASLLQPEGIYGPSFLTTANYFVIKQYNFSDL
YVLFVGHLSDRIAGAGPFATPWAASTQLRTTSVAAMQKHLTRVGLYADKIDGKAGMLTRAALGAYQKSAGLKVDCWPSEA
VLQSMGAAR
>Mature_408_residues
PSACRTLLLALLLSALTLQASAHAADAAFTQFIASLWPQAQAAGVSRATFERETRGLEPDYKLPDLALPGRPATGASAQA
EFVQVPASYVKEATIARLAADGQRLMQKYRAALDQIESRFGVPGTMVLALWGRETDYGRYTSPYDTLRVVATQAYAGRRK
DQYRDEFILALKILDEGAVARKDFRSSWAGATGLTQFLPSEYFKHGVDLDGDGRVDIWHSVPDALASAAKQLADKGWQPG
VRWAYEVRAPANADCTLGVPEVKKPIGEWLRTGFAPFARISAGEQAQPASLLQPEGIYGPSFLTTANYFVIKQYNFSDLY
VLFVGHLSDRIAGAGPFATPWAASTQLRTTSVAAMQKHLTRVGLYADKIDGKAGMLTRAALGAYQKSAGLKVDCWPSEAV
LQSMGAAR

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG2951

COG function: function code M; Membrane-bound lytic murein transglycosylase B

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor; Periplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789053, Length=249, Percent_Identity=27.710843373494, Blast_Score=80, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011757 [H]

Pfam domain/function: NA

EC number: 3.2.1.- [C]

Molecular weight: Translated: 44330; Mature: 44199

Theoretical pI: Translated: 9.11; Mature: 9.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSACRTLLLALLLSALTLQASAHAADAAFTQFIASLWPQAQAAGVSRATFERETRGLEP
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCHHHHHHHHHCCCCC
DYKLPDLALPGRPATGASAQAEFVQVPASYVKEATIARLAADGQRLMQKYRAALDQIESR
CCCCCCCCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FGVPGTMVLALWGRETDYGRYTSPYDTLRVVATQAYAGRRKDQYRDEFILALKILDEGAV
HCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHH
ARKDFRSSWAGATGLTQFLPSEYFKHGVDLDGDGRVDIWHSVPDALASAAKQLADKGWQP
HHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHCCCCC
GVRWAYEVRAPANADCTLGVPEVKKPIGEWLRTGFAPFARISAGEQAQPASLLQPEGIYG
CCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHCCCCCCCCCHHHCCCCCCCC
PSFLTTANYFVIKQYNFSDLYVLFVGHLSDRIAGAGPFATPWAASTQLRTTSVAAMQKHL
CHHHHHCCEEEEEECCCCCEEHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHH
TRVGLYADKIDGKAGMLTRAALGAYQKSAGLKVDCWPSEAVLQSMGAAR
HHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHCCCC
>Mature Secondary Structure 
PSACRTLLLALLLSALTLQASAHAADAAFTQFIASLWPQAQAAGVSRATFERETRGLEP
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCHHHHHHHHHCCCCC
DYKLPDLALPGRPATGASAQAEFVQVPASYVKEATIARLAADGQRLMQKYRAALDQIESR
CCCCCCCCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FGVPGTMVLALWGRETDYGRYTSPYDTLRVVATQAYAGRRKDQYRDEFILALKILDEGAV
HCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHH
ARKDFRSSWAGATGLTQFLPSEYFKHGVDLDGDGRVDIWHSVPDALASAAKQLADKGWQP
HHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHCCCCC
GVRWAYEVRAPANADCTLGVPEVKKPIGEWLRTGFAPFARISAGEQAQPASLLQPEGIYG
CCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHCCCCCCCCCHHHCCCCCCCC
PSFLTTANYFVIKQYNFSDLYVLFVGHLSDRIAGAGPFATPWAASTQLRTTSVAAMQKHL
CHHHHHCCEEEEEECCCCCEEHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHH
TRVGLYADKIDGKAGMLTRAALGAYQKSAGLKVDCWPSEAVLQSMGAAR
HHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

General reaction: Cleavage Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7476170; 7789526; 9205837; 9278503; 3553176; 9761817; 10570954; 10545329; 10684641 [H]