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The map label for this gene is purH

Identifier: 154245676

GI number: 154245676

Start: 1945839

End: 1947428

Strand: Direct

Name: purH

Synonym: Xaut_1732

Alternate gene names: 154245676

Gene position: 1945839-1947428 (Clockwise)

Preceding gene: 154245674

Following gene: 154245682

Centisome position: 36.65

GC content: 70.25

Gene sequence:

>1590_bases
ATGACCGCCGATATCCGTCCCGTGACCCGCGCCCTCCTCTCGGTGTCGGACAAGACGGGTCTCGTGCCGTTCGCGACAGC
CCTCGTTGCCCACGGGGTTGAGCTGGTCTCCACCGGCGGCACCGCCAAGGCCATCGCCGACGCCGGCCTGCCGGTGAAGG
ACGTGTCCGAGCTCACCGGCTTCCCGGAGATGATGGACGGCCGGGTGAAAACCCTGCATCCCAAGGTGCACGGCGGCATC
CTCGCCATCCGCGCCGACAAGGGCCACCAGGCGTCCCTCGCCGAGCACGCCATCGCCCCCATCGACCTCGTGGTGGTGAA
CCTCTATCCGTTCGAGGCGACCGTCGCCCGCAAGGCGGATTTCGAGGAGACCATCGAGAACATCGACATCGGCGGCCCCT
CCATGATCCGCGGCGCGGCCAAGAACCACAATGACGTGGCGGTGGTGGTGGAAGCCGCCGACTACGAGGCGGTGCTCGCT
GAGATCGCCGCCCACGGGGGCACCACCCTGCCCCTGCGCCGCAAGCTGGCCCAGAAGGCCTATGCCCGCACCGCCGCCTA
TGACGCGGCCATCTCCAACTGGTTCGCCCGCGAGCTGAAGGAGGAGGCCCCGGCCTTCCGCGCCTTCAGCGGCAAGCTCA
TCGAGGCCCTGCGCTACGGCGAGAACCCGCACCAGTCCGCCGCCTTCTACGCCTCGGACGAGATCCGTCCCGGCGTCGCC
ACGGCGCGTCAGGTGCAGGGCAAGCAGCTCTCCTACAACAACATCAACGACACCGACGCCGCCTTCGAGGCGGTGGCCGA
ATTCCACCCCGCCCGCACCGCCGCGGTGGTGATCGTGAAGCACGCCAACCCCTGCGGCGTGGCCGAAGGCGCCTCGCTGG
AGGAGGCCTATGCCAAGGCGCTGGCCTGCGATCCGGTGTCGGCCTTCGGCGGCATCATCGCGGTCAACCGCACGCTGGAT
GCGGCCTCCGCCCGCGCCATGGTGGAGATCTTCACCGAGGTGATCGTCGCCCCCGACGCAACCGACGAGGCCATCGCCGT
GGTGGCGGCCAAGAAGAACCTGCGCCTGCTGCTCACCGGCGGCCTGCCCGACCCGCGCGCGGGAGGCCTCACGGTGAAGA
CCGTGGCCGGCGGCCTGCTGGTGCAGGGCCGCGACAATGCCAGCGCCGACGACCTCGACCTGAAGGTGGTCACCAAGCGC
GCGCCCACCGACCGCGAGCTGACCGACCTGCGCTTCGCCTTCCGCGTGGCCAAGCATGTGAAGTCCAACACCATCATCTA
TGCGAAGGACCTCGCCACGGTGGGCATCGGCGCCGGGCAGATGAGCCGGGTGGACAGTGCCCGCATCGCCGCCCGCAAGG
CGCTGGACGCGGCCGAGGCCCTGGGCCTCAAGGAGCCGCTGACCAAGGGCTCGGTGGTGGCCTCCGATGCCTTCTTCCCC
TTCGCCGATGGCCTGCTGGCGGCGGTGGAGGCGGGTGCCACGGCGGTGATCCAGCCCGGCGGCTCCATGCGCGACAACGA
GGTGATCGCGGCGGCGGATGCGGCCGGCCTCGCCATGGTCTTCACCGGCGTGCGCCACTTCCGCCACTGA

Upstream 100 bases:

>100_bases
GCCCCTCCATACCCCTCATGCCCCCACCGGGGGCCTGCCGGCTGGCTCTGGCCGCCCAATGGTGATAGGCGAGCGGCAAT
CTCATCACCGGAGCCGCTCC

Downstream 100 bases:

>100_bases
GAAGCGGCGCGGAAAGCCCAGACAACATTACCTCGTCATGCCCGGCCTTGTGCCGGGCATCCACGCCGATCCGCTGGCGC
CCACGCGCGAAAGGACTTCC

Product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase [H]

Number of amino acids: Translated: 529; Mature: 528

Protein sequence:

>529_residues
MTADIRPVTRALLSVSDKTGLVPFATALVAHGVELVSTGGTAKAIADAGLPVKDVSELTGFPEMMDGRVKTLHPKVHGGI
LAIRADKGHQASLAEHAIAPIDLVVVNLYPFEATVARKADFEETIENIDIGGPSMIRGAAKNHNDVAVVVEAADYEAVLA
EIAAHGGTTLPLRRKLAQKAYARTAAYDAAISNWFARELKEEAPAFRAFSGKLIEALRYGENPHQSAAFYASDEIRPGVA
TARQVQGKQLSYNNINDTDAAFEAVAEFHPARTAAVVIVKHANPCGVAEGASLEEAYAKALACDPVSAFGGIIAVNRTLD
AASARAMVEIFTEVIVAPDATDEAIAVVAAKKNLRLLLTGGLPDPRAGGLTVKTVAGGLLVQGRDNASADDLDLKVVTKR
APTDRELTDLRFAFRVAKHVKSNTIIYAKDLATVGIGAGQMSRVDSARIAARKALDAAEALGLKEPLTKGSVVASDAFFP
FADGLLAAVEAGATAVIQPGGSMRDNEVIAAADAAGLAMVFTGVRHFRH

Sequences:

>Translated_529_residues
MTADIRPVTRALLSVSDKTGLVPFATALVAHGVELVSTGGTAKAIADAGLPVKDVSELTGFPEMMDGRVKTLHPKVHGGI
LAIRADKGHQASLAEHAIAPIDLVVVNLYPFEATVARKADFEETIENIDIGGPSMIRGAAKNHNDVAVVVEAADYEAVLA
EIAAHGGTTLPLRRKLAQKAYARTAAYDAAISNWFARELKEEAPAFRAFSGKLIEALRYGENPHQSAAFYASDEIRPGVA
TARQVQGKQLSYNNINDTDAAFEAVAEFHPARTAAVVIVKHANPCGVAEGASLEEAYAKALACDPVSAFGGIIAVNRTLD
AASARAMVEIFTEVIVAPDATDEAIAVVAAKKNLRLLLTGGLPDPRAGGLTVKTVAGGLLVQGRDNASADDLDLKVVTKR
APTDRELTDLRFAFRVAKHVKSNTIIYAKDLATVGIGAGQMSRVDSARIAARKALDAAEALGLKEPLTKGSVVASDAFFP
FADGLLAAVEAGATAVIQPGGSMRDNEVIAAADAAGLAMVFTGVRHFRH
>Mature_528_residues
TADIRPVTRALLSVSDKTGLVPFATALVAHGVELVSTGGTAKAIADAGLPVKDVSELTGFPEMMDGRVKTLHPKVHGGIL
AIRADKGHQASLAEHAIAPIDLVVVNLYPFEATVARKADFEETIENIDIGGPSMIRGAAKNHNDVAVVVEAADYEAVLAE
IAAHGGTTLPLRRKLAQKAYARTAAYDAAISNWFARELKEEAPAFRAFSGKLIEALRYGENPHQSAAFYASDEIRPGVAT
ARQVQGKQLSYNNINDTDAAFEAVAEFHPARTAAVVIVKHANPCGVAEGASLEEAYAKALACDPVSAFGGIIAVNRTLDA
ASARAMVEIFTEVIVAPDATDEAIAVVAAKKNLRLLLTGGLPDPRAGGLTVKTVAGGLLVQGRDNASADDLDLKVVTKRA
PTDRELTDLRFAFRVAKHVKSNTIIYAKDLATVGIGAGQMSRVDSARIAARKALDAAEALGLKEPLTKGSVVASDAFFPF
ADGLLAAVEAGATAVIQPGGSMRDNEVIAAADAAGLAMVFTGVRHFRH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family [H]

Homologues:

Organism=Homo sapiens, GI20127454, Length=474, Percent_Identity=39.2405063291139, Blast_Score=266, Evalue=3e-71,
Organism=Escherichia coli, GI1790439, Length=538, Percent_Identity=58.17843866171, Blast_Score=561, Evalue=1e-161,
Organism=Caenorhabditis elegans, GI71985564, Length=602, Percent_Identity=34.0531561461794, Blast_Score=293, Evalue=2e-79,
Organism=Caenorhabditis elegans, GI71985574, Length=306, Percent_Identity=27.7777777777778, Blast_Score=90, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6323056, Length=599, Percent_Identity=33.889816360601, Blast_Score=291, Evalue=2e-79,
Organism=Saccharomyces cerevisiae, GI6323768, Length=600, Percent_Identity=32.6666666666667, Blast_Score=282, Evalue=9e-77,
Organism=Drosophila melanogaster, GI24649832, Length=468, Percent_Identity=38.8888888888889, Blast_Score=274, Evalue=1e-73,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607 [H]

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS [H]

EC number: =2.1.2.3; =3.5.4.10 [H]

Molecular weight: Translated: 55358; Mature: 55226

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTADIRPVTRALLSVSDKTGLVPFATALVAHGVELVSTGGTAKAIADAGLPVKDVSELTG
CCCCHHHHHHHHHHCCCCCCCHHHHHHHHHCCHHEEECCCCHHHHHHCCCCHHHHHHHCC
FPEMMDGRVKTLHPKVHGGILAIRADKGHQASLAEHAIAPIDLVVVNLYPFEATVARKAD
CHHHHCCCEEEECCCCCCCEEEEEECCCCCHHHHHHCCCCEEEEEEEEECCHHHHHHCCC
FEETIENIDIGGPSMIRGAAKNHNDVAVVVEAADYEAVLAEIAAHGGTTLPLRRKLAQKA
HHHHHHHCCCCCHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHCCCCCCHHHHHHHHHH
YARTAAYDAAISNWFARELKEEAPAFRAFSGKLIEALRYGENPHQSAAFYASDEIRPGVA
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCHH
TARQVQGKQLSYNNINDTDAAFEAVAEFHPARTAAVVIVKHANPCGVAEGASLEEAYAKA
HHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCHHHHHHHH
LACDPVSAFGGIIAVNRTLDAASARAMVEIFTEVIVAPDATDEAIAVVAAKKNLRLLLTG
HCCCCHHHCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCEEEEEEC
GLPDPRAGGLTVKTVAGGLLVQGRDNASADDLDLKVVTKRAPTDRELTDLRFAFRVAKHV
CCCCCCCCCEEEEEECCCEEEECCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHH
KSNTIIYAKDLATVGIGAGQMSRVDSARIAARKALDAAEALGLKEPLTKGSVVASDAFFP
CCCEEEEEECCCEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCH
FADGLLAAVEAGATAVIQPGGSMRDNEVIAAADAAGLAMVFTGVRHFRH
HHHHHHHHHHCCCEEEECCCCCCCCCCEEEECCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
TADIRPVTRALLSVSDKTGLVPFATALVAHGVELVSTGGTAKAIADAGLPVKDVSELTG
CCCHHHHHHHHHHCCCCCCCHHHHHHHHHCCHHEEECCCCHHHHHHCCCCHHHHHHHCC
FPEMMDGRVKTLHPKVHGGILAIRADKGHQASLAEHAIAPIDLVVVNLYPFEATVARKAD
CHHHHCCCEEEECCCCCCCEEEEEECCCCCHHHHHHCCCCEEEEEEEEECCHHHHHHCCC
FEETIENIDIGGPSMIRGAAKNHNDVAVVVEAADYEAVLAEIAAHGGTTLPLRRKLAQKA
HHHHHHHCCCCCHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHCCCCCCHHHHHHHHHH
YARTAAYDAAISNWFARELKEEAPAFRAFSGKLIEALRYGENPHQSAAFYASDEIRPGVA
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCHH
TARQVQGKQLSYNNINDTDAAFEAVAEFHPARTAAVVIVKHANPCGVAEGASLEEAYAKA
HHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCHHHHHHHH
LACDPVSAFGGIIAVNRTLDAASARAMVEIFTEVIVAPDATDEAIAVVAAKKNLRLLLTG
HCCCCHHHCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCEEEEEEC
GLPDPRAGGLTVKTVAGGLLVQGRDNASADDLDLKVVTKRAPTDRELTDLRFAFRVAKHV
CCCCCCCCCEEEEEECCCEEEECCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHH
KSNTIIYAKDLATVGIGAGQMSRVDSARIAARKALDAAEALGLKEPLTKGSVVASDAFFP
CCCEEEEEECCCEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCH
FADGLLAAVEAGATAVIQPGGSMRDNEVIAAADAAGLAMVFTGVRHFRH
HHHHHHHHHHCCCEEEECCCCCCCCCCEEEECCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]