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The map label for this gene is folE

Identifier: 154245593

GI number: 154245593

Start: 1868240

End: 1868968

Strand: Reverse

Name: folE

Synonym: Xaut_1649

Alternate gene names: 154245593

Gene position: 1868968-1868240 (Counterclockwise)

Preceding gene: 154245595

Following gene: 154245592

Centisome position: 35.2

GC content: 66.26

Gene sequence:

>729_bases
ATGGACGCGGTTGTGAAGTTGTTCAAGGCGCAGGCCGAGAACGAAGAGCGTGAGCGCAAGCTCAAGGCGGAAGCCAAGGC
TGCCTCCAAGGCCGCGGCGCGGCCGGACGGTGCGCCCCCGGCGCCAAAAATTCATCCTCCCCTGCCGGATGGCACGCCCC
GCCCCTCGCGGCAGGAGGCGGAAGCCGCCGTGCGAACTCTTCTTGCCTATATCGGCGAGGATCCCGCCCGCGAGGGGCTG
CTCGACACGCCGAAGCGCGTGGTGAAGGCTTATGACGAGCTGTTCTCCGGCTATGGCGAGGACAGCGAGGAGTTGCTGGA
GCGTACCTTCGGCGAGATCGGCACCTTCGACGATTTCGTGCTGGTGCGCGACATTCCCTTCCACTCCCATTGCGAGCACC
ACATGGTGCCTTTCGTGGGCAAGGCGCACATCGCCTATTTCCCGGTGGAGCGGGTGGTGGGCCTGTCCAAGATCGCGCGG
GTGGTGGACATCTTCGCCCGCCGGCTGCAGACGCAGGAGCACCTGACCTCGCAGATCACCACCACTCTCGACGAGGGCCT
GCGCCCGCGCGGCGTGGCGGTGATGATCGAGGCCGAGCACATGTGCATGTCCATGCGCGGCATCACCAAGCAGGGCGTCT
CCACCCTCACCACCCAGTTCACCGGCGTGTTCCGGGACGACCCGGCCGAGCAGGTGCGCTTCATCACCCTGGTGCGCTCC
AAGTCCTGA

Upstream 100 bases:

>100_bases
GAGCGACACCGCGCATGCGTAGCCGACGACCGGTGACACTCCCGCTTCCACGGGCTGGCCCTGATGGGCTCTGCCGGGGG
CGGGTCGAACGGAGTTGGGC

Downstream 100 bases:

>100_bases
CTGCAGGTTCTGAAGGCGGGTCCTGACCACCGGTCCTGACCGGGCGTACTTGACGTTCAAACTTCATCGGGGCGTTCCTG
CCATGCAGGGACGCCCCTTT

Product: GTP cyclohydrolase I

Products: NA

Alternate protein names: GTP cyclohydrolase I; GTP-CH-I [H]

Number of amino acids: Translated: 242; Mature: 242

Protein sequence:

>242_residues
MDAVVKLFKAQAENEERERKLKAEAKAASKAAARPDGAPPAPKIHPPLPDGTPRPSRQEAEAAVRTLLAYIGEDPAREGL
LDTPKRVVKAYDELFSGYGEDSEELLERTFGEIGTFDDFVLVRDIPFHSHCEHHMVPFVGKAHIAYFPVERVVGLSKIAR
VVDIFARRLQTQEHLTSQITTTLDEGLRPRGVAVMIEAEHMCMSMRGITKQGVSTLTTQFTGVFRDDPAEQVRFITLVRS
KS

Sequences:

>Translated_242_residues
MDAVVKLFKAQAENEERERKLKAEAKAASKAAARPDGAPPAPKIHPPLPDGTPRPSRQEAEAAVRTLLAYIGEDPAREGL
LDTPKRVVKAYDELFSGYGEDSEELLERTFGEIGTFDDFVLVRDIPFHSHCEHHMVPFVGKAHIAYFPVERVVGLSKIAR
VVDIFARRLQTQEHLTSQITTTLDEGLRPRGVAVMIEAEHMCMSMRGITKQGVSTLTTQFTGVFRDDPAEQVRFITLVRS
KS
>Mature_242_residues
MDAVVKLFKAQAENEERERKLKAEAKAASKAAARPDGAPPAPKIHPPLPDGTPRPSRQEAEAAVRTLLAYIGEDPAREGL
LDTPKRVVKAYDELFSGYGEDSEELLERTFGEIGTFDDFVLVRDIPFHSHCEHHMVPFVGKAHIAYFPVERVVGLSKIAR
VVDIFARRLQTQEHLTSQITTTLDEGLRPRGVAVMIEAEHMCMSMRGITKQGVSTLTTQFTGVFRDDPAEQVRFITLVRS
KS

Specific function: Tetrahydrofolate biosynthesis; first step. [C]

COG id: COG0302

COG function: function code H; GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family [H]

Homologues:

Organism=Homo sapiens, GI66932968, Length=220, Percent_Identity=45.9090909090909, Blast_Score=186, Evalue=1e-47,
Organism=Homo sapiens, GI4503949, Length=220, Percent_Identity=45.9090909090909, Blast_Score=186, Evalue=1e-47,
Organism=Homo sapiens, GI66932970, Length=189, Percent_Identity=42.8571428571429, Blast_Score=140, Evalue=1e-33,
Organism=Homo sapiens, GI66932972, Length=178, Percent_Identity=44.9438202247191, Blast_Score=138, Evalue=3e-33,
Organism=Escherichia coli, GI1788476, Length=198, Percent_Identity=37.3737373737374, Blast_Score=114, Evalue=8e-27,
Organism=Caenorhabditis elegans, GI17560486, Length=180, Percent_Identity=48.8888888888889, Blast_Score=184, Evalue=3e-47,
Organism=Saccharomyces cerevisiae, GI6321706, Length=186, Percent_Identity=46.7741935483871, Blast_Score=167, Evalue=1e-42,
Organism=Drosophila melanogaster, GI24656782, Length=180, Percent_Identity=47.2222222222222, Blast_Score=164, Evalue=5e-41,
Organism=Drosophila melanogaster, GI24656791, Length=180, Percent_Identity=47.2222222222222, Blast_Score=163, Evalue=7e-41,
Organism=Drosophila melanogaster, GI24656796, Length=180, Percent_Identity=47.2222222222222, Blast_Score=163, Evalue=8e-41,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001474
- InterPro:   IPR020602
- InterPro:   IPR018234 [H]

Pfam domain/function: PF01227 GTP_cyclohydroI [H]

EC number: =3.5.4.16 [H]

Molecular weight: Translated: 26935; Mature: 26935

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: PS00859 GTP_CYCLOHYDROL_1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDAVVKLFKAQAENEERERKLKAEAKAASKAAARPDGAPPAPKIHPPLPDGTPRPSRQEA
CHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHH
EAAVRTLLAYIGEDPAREGLLDTPKRVVKAYDELFSGYGEDSEELLERTFGEIGTFDDFV
HHHHHHHHHHHCCCHHHCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCEE
LVRDIPFHSHCEHHMVPFVGKAHIAYFPVERVVGLSKIARVVDIFARRLQTQEHLTSQIT
EEECCCCHHCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TTLDEGLRPRGVAVMIEAEHMCMSMRGITKQGVSTLTTQFTGVFRDDPAEQVRFITLVRS
HHHHHCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHC
KS
CC
>Mature Secondary Structure
MDAVVKLFKAQAENEERERKLKAEAKAASKAAARPDGAPPAPKIHPPLPDGTPRPSRQEA
CHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHH
EAAVRTLLAYIGEDPAREGLLDTPKRVVKAYDELFSGYGEDSEELLERTFGEIGTFDDFV
HHHHHHHHHHHCCCHHHCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCEE
LVRDIPFHSHCEHHMVPFVGKAHIAYFPVERVVGLSKIARVVDIFARRLQTQEHLTSQIT
EEECCCCHHCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TTLDEGLRPRGVAVMIEAEHMCMSMRGITKQGVSTLTTQFTGVFRDDPAEQVRFITLVRS
HHHHHCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHC
KS
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA