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The map label for this gene is spoT [H]

Identifier: 154245595

GI number: 154245595

Start: 1869830

End: 1870438

Strand: Reverse

Name: spoT [H]

Synonym: Xaut_1651

Alternate gene names: 154245595

Gene position: 1870438-1869830 (Counterclockwise)

Preceding gene: 154245597

Following gene: 154245593

Centisome position: 35.23

GC content: 73.4

Gene sequence:

>609_bases
GTGACAAGCGACCCCGCAAACCCCGCAGCCGAACCGGCCGCACCCGCCCCGCCGCTGGCCTTGGTGGTGCGCGCCCTCGC
CTTCGCGACGCTGCGCCACACCGACCAGCGCCGCAAGGGCCGGCGCGCCCAGCCCTATGTCAACCATCTGGCGGAGGTCG
CCCTTCTGGTGAGCGAGGCCACCGGCGGGCAGGATGCCGCCCTCGTCGCCGCCGCCCTGCTCCATGACGTGGTGGAGGAC
CAGGACGTGGCCGCCGCCGAGATCGAGGCCACCTTCGGGTCCGAGGTCGCGGGCCTCGTCATGGAGGTCACCGACGACAA
GGCCCTTGAGGCCACGGAGCGCAAGCGACTTCAGGTGGCCCACGCCCCCCATCTGTCGGCCCGCGCCAAGGTGCTCAAGC
TCGCCGACAAGACCGCGAACCTGCGCTCCATCGCCGAGGATCCGCCCCAGGACTGGAGTCCCGCCCGCGCGCGGGCCTAT
GTGGAATGGGCCCGCGCCGTGGTGGAAGGCCTTGCCGGCGCCTGCCCGGCGCTGGAGTCCGCCTTCGATCAGGCCGCGGA
GGAAGCCATCGCCGCCATCGACACCCGCGCCGCGCACCCGGCCGACTGA

Upstream 100 bases:

>100_bases
CATCGTGGACGGCGCTGATGGCGTCATCCAATCCCACAAACGTGGCCGGGCCGTGTTGGTCCCGGTGACCGCCACGGTTC
CGCTCTGCGGAGGCGAACCC

Downstream 100 bases:

>100_bases
GGTCTCAGCCGGTGGCGGAAGCGGCTTCGTCGGCGCGCTGATCCCTGGCGGCCCGCGCGGCCTCGATCTGGTCGATGGCG
TCCACCACCGCGTCGAAGGT

Product: metal dependent phosphohydrolase

Products: NA

Alternate protein names: Penta-phosphate guanosine-3'-pyrophosphohydrolase; (ppGpp)ase [H]

Number of amino acids: Translated: 202; Mature: 201

Protein sequence:

>202_residues
MTSDPANPAAEPAAPAPPLALVVRALAFATLRHTDQRRKGRRAQPYVNHLAEVALLVSEATGGQDAALVAAALLHDVVED
QDVAAAEIEATFGSEVAGLVMEVTDDKALEATERKRLQVAHAPHLSARAKVLKLADKTANLRSIAEDPPQDWSPARARAY
VEWARAVVEGLAGACPALESAFDQAAEEAIAAIDTRAAHPAD

Sequences:

>Translated_202_residues
MTSDPANPAAEPAAPAPPLALVVRALAFATLRHTDQRRKGRRAQPYVNHLAEVALLVSEATGGQDAALVAAALLHDVVED
QDVAAAEIEATFGSEVAGLVMEVTDDKALEATERKRLQVAHAPHLSARAKVLKLADKTANLRSIAEDPPQDWSPARARAY
VEWARAVVEGLAGACPALESAFDQAAEEAIAAIDTRAAHPAD
>Mature_201_residues
TSDPANPAAEPAAPAPPLALVVRALAFATLRHTDQRRKGRRAQPYVNHLAEVALLVSEATGGQDAALVAAALLHDVVEDQ
DVAAAEIEATFGSEVAGLVMEVTDDKALEATERKRLQVAHAPHLSARAKVLKLADKTANLRSIAEDPPQDWSPARARAYV
EWARAVVEGLAGACPALESAFDQAAEEAIAAIDTRAAHPAD

Specific function: In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the degradation of ppGpp into G

COG id: COG0317

COG function: function code TK; Guanosine polyphosphate pyrophosphohydrolases/synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HD domain [H]

Homologues:

Organism=Homo sapiens, GI38348360, Length=131, Percent_Identity=48.0916030534351, Blast_Score=113, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI25143118, Length=191, Percent_Identity=37.696335078534, Blast_Score=127, Evalue=6e-30,
Organism=Drosophila melanogaster, GI24650996, Length=167, Percent_Identity=44.9101796407186, Blast_Score=149, Evalue=2e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR012675
- InterPro:   IPR003607
- InterPro:   IPR007685
- InterPro:   IPR004811
- InterPro:   IPR004095
- InterPro:   IPR012676 [H]

Pfam domain/function: PF01842 ACT; PF04607 RelA_SpoT; PF02824 TGS [H]

EC number: =3.1.7.2 [H]

Molecular weight: Translated: 21242; Mature: 21111

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSDPANPAAEPAAPAPPLALVVRALAFATLRHTDQRRKGRRAQPYVNHLAEVALLVSEA
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHC
TGGQDAALVAAALLHDVVEDQDVAAAEIEATFGSEVAGLVMEVTDDKALEATERKRLQVA
CCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
HAPHLSARAKVLKLADKTANLRSIAEDPPQDWSPARARAYVEWARAVVEGLAGACPALES
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHH
AFDQAAEEAIAAIDTRAAHPAD
HHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TSDPANPAAEPAAPAPPLALVVRALAFATLRHTDQRRKGRRAQPYVNHLAEVALLVSEA
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHC
TGGQDAALVAAALLHDVVEDQDVAAAEIEATFGSEVAGLVMEVTDDKALEATERKRLQVA
CCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
HAPHLSARAKVLKLADKTANLRSIAEDPPQDWSPARARAYVEWARAVVEGLAGACPALES
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHH
AFDQAAEEAIAAIDTRAAHPAD
HHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]