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The map label for this gene is dosC [H]

Identifier: 154245372

GI number: 154245372

Start: 1632287

End: 1633561

Strand: Reverse

Name: dosC [H]

Synonym: Xaut_1425

Alternate gene names: 154245372

Gene position: 1633561-1632287 (Counterclockwise)

Preceding gene: 154245373

Following gene: 154245371

Centisome position: 30.77

GC content: 67.84

Gene sequence:

>1275_bases
ATGGCCGGCTTCAACGTCTTCCTGCTCGTCATCGAGATGGCGGTGTTTTTCACCGCCATGCTGGCCCTGTTTCGCCTGCG
CGAGACGCTGGGGCTGGGGGTCTTCTTCTGCGCCCTGGGCAGCTTCCATTTCATGGAGACCTACCTGGCGGCCGCCTATT
ATGTGCAGCTGCCCTTCGGCATCTCCCTGTCGCCGGGGTCGGTGGTGCTGTTCTCCGGCAAGCTGGCGCTGATCCTGCTC
ATCTATATCCGCGAGGATGCCCTCGTCGCCCGTCAGCCGATCTACGGGCTGATCATCGGCAACATCCTCCTGATTGGCCT
CGTCGCCCTGCTGCGCCAGCATGATGCGGTGGCGTCGCCCGGCGTGCCGGCCGACCTGTCCTTCATGGATGCCATGGGCC
TGCTGATGATGTGGGGCACGCTGCTGCTCTTCATTGATTCCATCGCCATGATCCTGATCTACGAGCGCATCGCGCGACCG
CTGCGGCGCAGGCCGGTTCTCGCCATCTGGATCACGCTGGCGCTGGTTCTCACCTTCGACCAGGTCGCGTTCTTCGGTGT
GCTCTCCCTCGCCATGGGCGTGCCGCTCACGGCGGGGATCGGCGGCTGGCTGGGCAAGGTGGTGGCGGCGGGGTTCTATG
CCGCCATGCTCGGCCAGTACCTCGCCCGCTTCGAATTGGCCGGTAACACACCGCGCCGGACCATCACCGACGTGTTCCAC
GCCCTCACCTACCGCCAGCGCTACGAGGATCTGAAGCAGGAATCCCGCCGCGACCCCCTCACCAACGTGCTGCATCGCGG
CCAGCTGGAGCCGCTGGGCCGCGACCTGCTCGCCGTGGCGCGCACCACCGGGCGGCCGGTGAGCCTGCTGCTCATCGACG
TGGACGATTTCAAGGACGTGAACGACCAGTTGGGACACCCCACCGGCGACGAAGTGCTGCGCCGCCTCGCCCGCGGCCTG
ACAGAGAGCCTGCGGCAGGCAGATCACGTGGTGCGCTATGGCGGCGACGAGTTCGCCGTCTTCGCCCCTGGCGTGGGCAA
TGCGGGGGCCTTGTCGCTCGCGGCCCAGCTGGGCCGGCGCGTCGGCGCCATGACGCTGCCCCCGGGGGTAGCGCGGCAGA
CCCTCTCCATCGGCATCGCCAGCGCTCCCGAGGACGGCAGCACCTTGACGGAGCTGCTGGATGCGGCCGACAAGCGCCTC
TACGCGGCGAAGGCGGCCGGGCGGAACCGCGCAGCGGGCGGACCCGAAGCCGACGCCCTCAAGATGAACGGATGA

Upstream 100 bases:

>100_bases
CCCAACCGCCGGGCCAACTTGCCGCATCTCCTGTTTTCTTCCGCCCGCCGCCTGCTAGGATCGCGGCCGGCCTGCAATTC
TGCGGGTGCAGCGATCCATC

Downstream 100 bases:

>100_bases
TTGCGCCCCCCATGCTCGAAGGCCTCCCCCCCAACGACGCCACCCATGTGATGCGGGTGGAAGCTTCCCGCGTCGCCGCC
CAGCGCATCTCCGACATGAT

Product: diguanylate cyclase

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 424; Mature: 423

Protein sequence:

>424_residues
MAGFNVFLLVIEMAVFFTAMLALFRLRETLGLGVFFCALGSFHFMETYLAAAYYVQLPFGISLSPGSVVLFSGKLALILL
IYIREDALVARQPIYGLIIGNILLIGLVALLRQHDAVASPGVPADLSFMDAMGLLMMWGTLLLFIDSIAMILIYERIARP
LRRRPVLAIWITLALVLTFDQVAFFGVLSLAMGVPLTAGIGGWLGKVVAAGFYAAMLGQYLARFELAGNTPRRTITDVFH
ALTYRQRYEDLKQESRRDPLTNVLHRGQLEPLGRDLLAVARTTGRPVSLLLIDVDDFKDVNDQLGHPTGDEVLRRLARGL
TESLRQADHVVRYGGDEFAVFAPGVGNAGALSLAAQLGRRVGAMTLPPGVARQTLSIGIASAPEDGSTLTELLDAADKRL
YAAKAAGRNRAAGGPEADALKMNG

Sequences:

>Translated_424_residues
MAGFNVFLLVIEMAVFFTAMLALFRLRETLGLGVFFCALGSFHFMETYLAAAYYVQLPFGISLSPGSVVLFSGKLALILL
IYIREDALVARQPIYGLIIGNILLIGLVALLRQHDAVASPGVPADLSFMDAMGLLMMWGTLLLFIDSIAMILIYERIARP
LRRRPVLAIWITLALVLTFDQVAFFGVLSLAMGVPLTAGIGGWLGKVVAAGFYAAMLGQYLARFELAGNTPRRTITDVFH
ALTYRQRYEDLKQESRRDPLTNVLHRGQLEPLGRDLLAVARTTGRPVSLLLIDVDDFKDVNDQLGHPTGDEVLRRLARGL
TESLRQADHVVRYGGDEFAVFAPGVGNAGALSLAAQLGRRVGAMTLPPGVARQTLSIGIASAPEDGSTLTELLDAADKRL
YAAKAAGRNRAAGGPEADALKMNG
>Mature_423_residues
AGFNVFLLVIEMAVFFTAMLALFRLRETLGLGVFFCALGSFHFMETYLAAAYYVQLPFGISLSPGSVVLFSGKLALILLI
YIREDALVARQPIYGLIIGNILLIGLVALLRQHDAVASPGVPADLSFMDAMGLLMMWGTLLLFIDSIAMILIYERIARPL
RRRPVLAIWITLALVLTFDQVAFFGVLSLAMGVPLTAGIGGWLGKVVAAGFYAAMLGQYLARFELAGNTPRRTITDVFHA
LTYRQRYEDLKQESRRDPLTNVLHRGQLEPLGRDLLAVARTTGRPVSLLLIDVDDFKDVNDQLGHPTGDEVLRRLARGLT
ESLRQADHVVRYGGDEFAVFAPGVGNAGALSLAAQLGRRVGAMTLPPGVARQTLSIGIASAPEDGSTLTELLDAADKRLY
AAKAAGRNRAAGGPEADALKMNG

Specific function: Overexpression leads to an increased level of c-di-GMP, which leads to changes in the cell surface, to abnormal cell division, increased biofilm formation and decreased swimming (the latter 2 in strain W3110). In a strain able to produce cellulose (strain

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=182, Percent_Identity=36.8131868131868, Blast_Score=108, Evalue=6e-25,
Organism=Escherichia coli, GI87081881, Length=178, Percent_Identity=39.8876404494382, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1786584, Length=168, Percent_Identity=36.9047619047619, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1787262, Length=168, Percent_Identity=32.1428571428571, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI87082007, Length=171, Percent_Identity=35.0877192982456, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1788381, Length=178, Percent_Identity=32.5842696629214, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI87081974, Length=167, Percent_Identity=31.1377245508982, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1787802, Length=174, Percent_Identity=33.9080459770115, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1787541, Length=170, Percent_Identity=31.7647058823529, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1787056, Length=153, Percent_Identity=35.9477124183007, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1787816, Length=136, Percent_Identity=34.5588235294118, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1788085, Length=174, Percent_Identity=27.0114942528736, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR012292
- InterPro:   IPR009050 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 45875; Mature: 45743

Theoretical pI: Translated: 8.84; Mature: 8.84

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGFNVFLLVIEMAVFFTAMLALFRLRETLGLGVFFCALGSFHFMETYLAAAYYVQLPFG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCC
ISLSPGSVVLFSGKLALILLIYIREDALVARQPIYGLIIGNILLIGLVALLRQHDAVASP
EEECCCCEEEECCCHHHHHHHHHHCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
GVPADLSFMDAMGLLMMWGTLLLFIDSIAMILIYERIARPLRRRPVLAIWITLALVLTFD
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
QVAFFGVLSLAMGVPLTAGIGGWLGKVVAAGFYAAMLGQYLARFELAGNTPRRTITDVFH
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
ALTYRQRYEDLKQESRRDPLTNVLHRGQLEPLGRDLLAVARTTGRPVSLLLIDVDDFKDV
HHHHHHHHHHHHHHHCCCHHHHHHHCCCCCHHHHHHHHHHHCCCCCEEEEEEECHHHHCH
NDQLGHPTGDEVLRRLARGLTESLRQADHVVRYGGDEFAVFAPGVGNAGALSLAAQLGRR
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHH
VGAMTLPPGVARQTLSIGIASAPEDGSTLTELLDAADKRLYAAKAAGRNRAAGGPEADAL
CCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
KMNG
CCCC
>Mature Secondary Structure 
AGFNVFLLVIEMAVFFTAMLALFRLRETLGLGVFFCALGSFHFMETYLAAAYYVQLPFG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCC
ISLSPGSVVLFSGKLALILLIYIREDALVARQPIYGLIIGNILLIGLVALLRQHDAVASP
EEECCCCEEEECCCHHHHHHHHHHCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
GVPADLSFMDAMGLLMMWGTLLLFIDSIAMILIYERIARPLRRRPVLAIWITLALVLTFD
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
QVAFFGVLSLAMGVPLTAGIGGWLGKVVAAGFYAAMLGQYLARFELAGNTPRRTITDVFH
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
ALTYRQRYEDLKQESRRDPLTNVLHRGQLEPLGRDLLAVARTTGRPVSLLLIDVDDFKDV
HHHHHHHHHHHHHHHCCCHHHHHHHCCCCCHHHHHHHHHHHCCCCCEEEEEEECHHHHCH
NDQLGHPTGDEVLRRLARGLTESLRQADHVVRYGGDEFAVFAPGVGNAGALSLAAQLGRR
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHH
VGAMTLPPGVARQTLSIGIASAPEDGSTLTELLDAADKRLYAAKAAGRNRAAGGPEADAL
CCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
KMNG
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]