Click here to switch to the map view.

The map label for this gene is aroA [H]

Identifier: 154245060

GI number: 154245060

Start: 1281079

End: 1282449

Strand: Reverse

Name: aroA [H]

Synonym: Xaut_1112

Alternate gene names: 154245060

Gene position: 1282449-1281079 (Counterclockwise)

Preceding gene: 154245062

Following gene: 154245059

Centisome position: 24.16

GC content: 73.3

Gene sequence:

>1371_bases
ATGTCCGCCACCCGCAGCGCCGCTCCCGCCTCCGGCCACGGCGACGGCCCGGCTCAGCCCGCGACCGCCCTTCGCTCCCC
CGCGCTCACCGGCCGCGTGCGTGTCCCCGGTGACAAGTCCGTGTCCCACCGCGCGCTGATCTTCGGCCTGTTCGCGGAGG
GTATCACCCGCATCACCGGCCTGCTGGAAGGCGAGGACGTGCTGAACACCGCCAAGGCCTGCGCCGCGCTCGGCGCGCGC
GTCACCCGCCGCGGCCCCGGCGAGTGGGAGGTGGAGGGCGCCGGCGCCGACGGGCTGTCCAGCCCCGCCGCCCCGCTGGA
TTTCGGCAATTCCGGTACCGGCGTGCGCCTCATGATGGGCGCGGTGGCCGGGCAGGACGTGACCGCCACCTTCGACGGCG
ACGCCAGCCTCAGGCGGCGCCCGATGAAGCGCGTGCTGGACCCGCTCACCGCCATGGGGGTGGAGATCGTGGACAGCGCC
GAGGGCGGGCGCCTGCCCCTCACCCTCAAGGGCAGCGACAGGACGAAGGCCATCACCTACGAAACCCCCATGCCGTCGGC
GCAGGTGAAGTCCGCCGTGCTGCTGGCCGGGCTCGGCGCCGCCGGCGAGACCGTGGTGATCGAGCGCGAGGCCACCCGCG
ACCACACCGAGCGCATGCTCGCCCATTTCGGCGCCGACGTGCGGGTGGAACCCCACGGCGCCCACGGCCGCCGCATCGCC
CTGAAGGGCCGGCCGACGCTCGTGGCCGCGCCGGTGGACGTGCCGGCCGACCCCTCCTCCGCCGCCTTCCCGCTGGTGGC
GGCGCTGATCGTGCCCGGCTCCGACGTGACCTTGACCGACGTGATGATGAACCCGCTGCGCACCGGCCTCATCACCACGC
TGCTGGAAATGGGCGCCAGCATCGAGGTGGTGGCCGAGCGCACCGAGGGCGGCGAGCGCGTCGCCGACCTGCGCGTGCGC
CATTCCCGCCTGAAGGGCGTGGAGGTGCCGCCCGAGCGGGCGCCGGCCATGATCGACGAATATCCCATCCTCGCCGTGGC
CGCCGCCTTTGCCGAGGGCGTCACCGCCATGCGCGGCCTCTCCGAGCTCAGGGTGAAGGAAAGCGATCGCCTGGCGGCGG
TGGCAGACGGCCTTGCCGCCTGCGGCGTGGCCCATGAGGTGGTGGGGGACGACCTGCTCGTGACCGGCGCCACCACGGTC
AAGGGCGGCGGTCCGGTCGCCACCCACATGGATCACCGCATCGCCATGTCGTTCCTGGTGCTGGGCCTTGCCAGCGAGCA
GGGCGTGAGCGTGGACGACGTGGCCTTCATCGCCACCTCCTTCCCCACCTTCATGCCCATGATGCGCGGCCTCGGCGCCG
TCATTTCGTGA

Upstream 100 bases:

>100_bases
GAGGCGACCGGATCCGGCTTTTCGCAAGCCGGCCTATACAACAAGAATCCGTCGCCCCGGACGTCATCGATTAAGACGCC
AACGCTTGAGGAAGGCCCCC

Downstream 100 bases:

>100_bases
AGTGACAGCCGTGGTCATCGCCATCGACGGGCCGGCGGCCTCCGGCAAGGGCACGCTGGCCCGCCGGCTCGCCGCTTATT
ACGGCCTGCCCCACCTCGAC

Product: 3-phosphoshikimate 1-carboxyvinyltransferase

Products: NA

Alternate protein names: 5-enolpyruvylshikimate-3-phosphate synthase; EPSP synthase; EPSPS [H]

Number of amino acids: Translated: 456; Mature: 455

Protein sequence:

>456_residues
MSATRSAAPASGHGDGPAQPATALRSPALTGRVRVPGDKSVSHRALIFGLFAEGITRITGLLEGEDVLNTAKACAALGAR
VTRRGPGEWEVEGAGADGLSSPAAPLDFGNSGTGVRLMMGAVAGQDVTATFDGDASLRRRPMKRVLDPLTAMGVEIVDSA
EGGRLPLTLKGSDRTKAITYETPMPSAQVKSAVLLAGLGAAGETVVIEREATRDHTERMLAHFGADVRVEPHGAHGRRIA
LKGRPTLVAAPVDVPADPSSAAFPLVAALIVPGSDVTLTDVMMNPLRTGLITTLLEMGASIEVVAERTEGGERVADLRVR
HSRLKGVEVPPERAPAMIDEYPILAVAAAFAEGVTAMRGLSELRVKESDRLAAVADGLAACGVAHEVVGDDLLVTGATTV
KGGGPVATHMDHRIAMSFLVLGLASEQGVSVDDVAFIATSFPTFMPMMRGLGAVIS

Sequences:

>Translated_456_residues
MSATRSAAPASGHGDGPAQPATALRSPALTGRVRVPGDKSVSHRALIFGLFAEGITRITGLLEGEDVLNTAKACAALGAR
VTRRGPGEWEVEGAGADGLSSPAAPLDFGNSGTGVRLMMGAVAGQDVTATFDGDASLRRRPMKRVLDPLTAMGVEIVDSA
EGGRLPLTLKGSDRTKAITYETPMPSAQVKSAVLLAGLGAAGETVVIEREATRDHTERMLAHFGADVRVEPHGAHGRRIA
LKGRPTLVAAPVDVPADPSSAAFPLVAALIVPGSDVTLTDVMMNPLRTGLITTLLEMGASIEVVAERTEGGERVADLRVR
HSRLKGVEVPPERAPAMIDEYPILAVAAAFAEGVTAMRGLSELRVKESDRLAAVADGLAACGVAHEVVGDDLLVTGATTV
KGGGPVATHMDHRIAMSFLVLGLASEQGVSVDDVAFIATSFPTFMPMMRGLGAVIS
>Mature_455_residues
SATRSAAPASGHGDGPAQPATALRSPALTGRVRVPGDKSVSHRALIFGLFAEGITRITGLLEGEDVLNTAKACAALGARV
TRRGPGEWEVEGAGADGLSSPAAPLDFGNSGTGVRLMMGAVAGQDVTATFDGDASLRRRPMKRVLDPLTAMGVEIVDSAE
GGRLPLTLKGSDRTKAITYETPMPSAQVKSAVLLAGLGAAGETVVIEREATRDHTERMLAHFGADVRVEPHGAHGRRIAL
KGRPTLVAAPVDVPADPSSAAFPLVAALIVPGSDVTLTDVMMNPLRTGLITTLLEMGASIEVVAERTEGGERVADLRVRH
SRLKGVEVPPERAPAMIDEYPILAVAAAFAEGVTAMRGLSELRVKESDRLAAVADGLAACGVAHEVVGDDLLVTGATTVK
GGGPVATHMDHRIAMSFLVLGLASEQGVSVDDVAFIATSFPTFMPMMRGLGAVIS

Specific function: Aromatic amino acids biosynthesis; shikimate pathway; sixth step. [C]

COG id: COG0128

COG function: function code E; 5-enolpyruvylshikimate-3-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the EPSP synthase family [H]

Homologues:

Organism=Escherichia coli, GI1787137, Length=429, Percent_Identity=28.6713286713287, Blast_Score=111, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6320332, Length=436, Percent_Identity=29.8165137614679, Blast_Score=114, Evalue=3e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001986
- InterPro:   IPR006264
- InterPro:   IPR023193
- InterPro:   IPR013792 [H]

Pfam domain/function: PF00275 EPSP_synthase [H]

EC number: =2.5.1.19 [H]

Molecular weight: Translated: 47054; Mature: 46922

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: PS00104 EPSP_SYNTHASE_1 ; PS00885 EPSP_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSATRSAAPASGHGDGPAQPATALRSPALTGRVRVPGDKSVSHRALIFGLFAEGITRITG
CCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHH
LLEGEDVLNTAKACAALGARVTRRGPGEWEVEGAGADGLSSPAAPLDFGNSGTGVRLMMG
HCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCEEEEE
AVAGQDVTATFDGDASLRRRPMKRVLDPLTAMGVEIVDSAEGGRLPLTLKGSDRTKAITY
HHCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCEEECCCCCEEEEEEECCCCCEEEEE
ETPMPSAQVKSAVLLAGLGAAGETVVIEREATRDHTERMLAHFGADVRVEPHGAHGRRIA
ECCCCHHHHHHHHHEEECCCCCCEEEEECHHHHHHHHHHHHHCCCCEEECCCCCCCCEEE
LKGRPTLVAAPVDVPADPSSAAFPLVAALIVPGSDVTLTDVMMNPLRTGLITTLLEMGAS
ECCCCEEEECCCCCCCCCCCCHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHCCCC
IEVVAERTEGGERVADLRVRHSRLKGVEVPPERAPAMIDEYPILAVAAAFAEGVTAMRGL
EEEEEECCCCCHHHHHHHHHHHHCCCCCCCHHHCCCHHHCCHHHHHHHHHHHHHHHHHCH
SELRVKESDRLAAVADGLAACGVAHEVVGDDLLVTGATTVKGGGPVATHMDHRIAMSFLV
HHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCEEECCCCCCHHHHHHHHHHHHHH
LGLASEQGVSVDDVAFIATSFPTFMPMMRGLGAVIS
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SATRSAAPASGHGDGPAQPATALRSPALTGRVRVPGDKSVSHRALIFGLFAEGITRITG
CCCCCCCCCCCCCCCCCCCHHHHCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHH
LLEGEDVLNTAKACAALGARVTRRGPGEWEVEGAGADGLSSPAAPLDFGNSGTGVRLMMG
HCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCEEEEE
AVAGQDVTATFDGDASLRRRPMKRVLDPLTAMGVEIVDSAEGGRLPLTLKGSDRTKAITY
HHCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCEEECCCCCEEEEEEECCCCCEEEEE
ETPMPSAQVKSAVLLAGLGAAGETVVIEREATRDHTERMLAHFGADVRVEPHGAHGRRIA
ECCCCHHHHHHHHHEEECCCCCCEEEEECHHHHHHHHHHHHHCCCCEEECCCCCCCCEEE
LKGRPTLVAAPVDVPADPSSAAFPLVAALIVPGSDVTLTDVMMNPLRTGLITTLLEMGAS
ECCCCEEEECCCCCCCCCCCCHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHCCCC
IEVVAERTEGGERVADLRVRHSRLKGVEVPPERAPAMIDEYPILAVAAAFAEGVTAMRGL
EEEEEECCCCCHHHHHHHHHHHHCCCCCCCHHHCCCHHHCCHHHHHHHHHHHHHHHHHCH
SELRVKESDRLAAVADGLAACGVAHEVVGDDLLVTGATTVKGGGPVATHMDHRIAMSFLV
HHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCEEECCCCCCHHHHHHHHHHHHHH
LGLASEQGVSVDDVAFIATSFPTFMPMMRGLGAVIS
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]