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The map label for this gene is hyi [C]

Identifier: 154244976

GI number: 154244976

Start: 1178823

End: 1179626

Strand: Reverse

Name: hyi [C]

Synonym: Xaut_1026

Alternate gene names: 154244976

Gene position: 1179626-1178823 (Counterclockwise)

Preceding gene: 154244977

Following gene: 154244975

Centisome position: 22.22

GC content: 68.03

Gene sequence:

>804_bases
ATGCCGCGCTTTGCCGCCAACCTCACCATGATGTTCACCGAGTGGTCGTTCCTCGACCGCTTCGCCGCCGCCGCCGATGC
CGGCTTCACCGCGGTGGAATTCCTCTTCCCCTACGAGCATGCGCCGGAAGCCGTGGGCGAACGCCTCGCCCGCAACGGCC
TGACCCAGGCCCTGTTCAACCTGCCCCCCGGCGATTGGGCGGCTGGCGAGCGCGGCATCGCTGCCTTCCCCGAGCGCTTC
GACGAACTGAAGGCAGGCGTTGCGACCGGCCTCACCTATGCCGCAGCCACCGGCGTGAAGCGCCTGCATCTCATGTCCGG
CCTTGCCGACCGCGCCGACCCCAAGGCCCGCGCCGCCTATCGCGAGGCGGTGGTCTACGCCGCCGGCGCGCTGGCGGAGA
AGGGTCTCGACCTGGTGCTGGAGCCCATCAACGGCCGCAACATGCCGGGCTATTTCCTCAACGATTTCGCCTTCACCGAG
GCGCTCATCGCCGAGCTGGGCCTGCCCAACCTGAAGCTCCAGTTCGACATCTATCACTGCCAGATCATCCATGGCGACGT
GACCATGCGGCTGCGCCAGCTCATGCCCATCACCGGCCATGTGCAGATCGCCAGCGTGCCGTCCCGCAACGAGCCGGACG
GCGAGGAGCTGAACTATCCGTTCCTGTTCGCGGAGCTGGATCGCCTGGGATATGACGGCTTCGTGGGCTGCGAATACAAC
CCGCGCGCCGGCACGCTGAGCGGCCTGAGCTGGTTCGCGCCCTATGCCGCCGCCCCGGCCCTCCAGGCGGGAGCGAACGG
ATGA

Upstream 100 bases:

>100_bases
AACCCTGCTTGCGTCCCTGCGCGGATTGGTGCGTCCTTCACGCCGCACCGACCGCCGCGACCCGACCTTTCGGAAGATCC
CACCTTTTCGGACAATTCCC

Downstream 100 bases:

>100_bases
CCCTCGCCCTCGGCACCATCGCCGACGACTATACCGGCGCCTCCGACCTCGCCAACACCTTGGCCAAGAACGGCCTGCGC
ACCGTGCAGACCGTGGGCAT

Product: hydroxypyruvate isomerase

Products: 2-hydroxy-3-oxopropanoate

Alternate protein names: ORF4 [H]

Number of amino acids: Translated: 267; Mature: 266

Protein sequence:

>267_residues
MPRFAANLTMMFTEWSFLDRFAAAADAGFTAVEFLFPYEHAPEAVGERLARNGLTQALFNLPPGDWAAGERGIAAFPERF
DELKAGVATGLTYAAATGVKRLHLMSGLADRADPKARAAYREAVVYAAGALAEKGLDLVLEPINGRNMPGYFLNDFAFTE
ALIAELGLPNLKLQFDIYHCQIIHGDVTMRLRQLMPITGHVQIASVPSRNEPDGEELNYPFLFAELDRLGYDGFVGCEYN
PRAGTLSGLSWFAPYAAAPALQAGANG

Sequences:

>Translated_267_residues
MPRFAANLTMMFTEWSFLDRFAAAADAGFTAVEFLFPYEHAPEAVGERLARNGLTQALFNLPPGDWAAGERGIAAFPERF
DELKAGVATGLTYAAATGVKRLHLMSGLADRADPKARAAYREAVVYAAGALAEKGLDLVLEPINGRNMPGYFLNDFAFTE
ALIAELGLPNLKLQFDIYHCQIIHGDVTMRLRQLMPITGHVQIASVPSRNEPDGEELNYPFLFAELDRLGYDGFVGCEYN
PRAGTLSGLSWFAPYAAAPALQAGANG
>Mature_266_residues
PRFAANLTMMFTEWSFLDRFAAAADAGFTAVEFLFPYEHAPEAVGERLARNGLTQALFNLPPGDWAAGERGIAAFPERFD
ELKAGVATGLTYAAATGVKRLHLMSGLADRADPKARAAYREAVVYAAGALAEKGLDLVLEPINGRNMPGYFLNDFAFTEA
LIAELGLPNLKLQFDIYHCQIIHGDVTMRLRQLMPITGHVQIASVPSRNEPDGEELNYPFLFAELDRLGYDGFVGCEYNP
RAGTLSGLSWFAPYAAAPALQAGANG

Specific function: Catalyzes The Reversible Isomerization Between Hydroxypyruvate And 2-Hydroxy-3-Oxopropanoate (Also Termed Tartronate Semialdehyde). [C]

COG id: COG3622

COG function: function code G; Hydroxypyruvate isomerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hyi family [H]

Homologues:

Organism=Homo sapiens, GI300068962, Length=262, Percent_Identity=45.8015267175573, Blast_Score=207, Evalue=1e-53,
Organism=Homo sapiens, GI300068964, Length=243, Percent_Identity=44.8559670781893, Blast_Score=180, Evalue=1e-45,
Organism=Escherichia coli, GI1786718, Length=257, Percent_Identity=43.9688715953307, Blast_Score=220, Evalue=9e-59,
Organism=Escherichia coli, GI1789095, Length=254, Percent_Identity=42.9133858267717, Blast_Score=207, Evalue=6e-55,
Organism=Caenorhabditis elegans, GI17552022, Length=240, Percent_Identity=36.6666666666667, Blast_Score=161, Evalue=4e-40,
Organism=Drosophila melanogaster, GI17530883, Length=257, Percent_Identity=32.6848249027237, Blast_Score=143, Evalue=1e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013022
- InterPro:   IPR012307 [H]

Pfam domain/function: PF01261 AP_endonuc_2 [H]

EC number: 5.3.1.22

Molecular weight: Translated: 28995; Mature: 28864

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRFAANLTMMFTEWSFLDRFAAAADAGFTAVEFLFPYEHAPEAVGERLARNGLTQALFN
CCCHHCCEEEEEHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHC
LPPGDWAAGERGIAAFPERFDELKAGVATGLTYAAATGVKRLHLMSGLADRADPKARAAY
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
REAVVYAAGALAEKGLDLVLEPINGRNMPGYFLNDFAFTEALIAELGLPNLKLQFDIYHC
HHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEEEEE
QIIHGDVTMRLRQLMPITGHVQIASVPSRNEPDGEELNYPFLFAELDRLGYDGFVGCEYN
EEEECHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHCCCCCEECCCCC
PRAGTLSGLSWFAPYAAAPALQAGANG
CCCCCCCCHHHHHHHHHCCHHHCCCCC
>Mature Secondary Structure 
PRFAANLTMMFTEWSFLDRFAAAADAGFTAVEFLFPYEHAPEAVGERLARNGLTQALFN
CCHHCCEEEEEHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHC
LPPGDWAAGERGIAAFPERFDELKAGVATGLTYAAATGVKRLHLMSGLADRADPKARAAY
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
REAVVYAAGALAEKGLDLVLEPINGRNMPGYFLNDFAFTEALIAELGLPNLKLQFDIYHC
HHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEEEEE
QIIHGDVTMRLRQLMPITGHVQIASVPSRNEPDGEELNYPFLFAELDRLGYDGFVGCEYN
EEEECHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHCCCCCEECCCCC
PRAGTLSGLSWFAPYAAAPALQAGANG
CCCCCCCCHHHHHHHHHCCHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 10 {hydroxypyruvate}} [C]

Substrates: hydroxypyruvate

Specific reaction: hydroxypyruvate = 2-hydroxy-3-oxopropanoate; Other Name(s): hydroxypyruvate ketol-isomerase

General reaction: Intramolecular oxidoreduction; Isomerization [C]

Inhibitor: Cu2+; Fe2+; Hydroxy l amine; Iodoacetate; NaCN [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7851418 [H]