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The map label for this gene is ygbJ [H]

Identifier: 154244977

GI number: 154244977

Start: 1179742

End: 1180734

Strand: Reverse

Name: ygbJ [H]

Synonym: Xaut_1027

Alternate gene names: 154244977

Gene position: 1180734-1179742 (Counterclockwise)

Preceding gene: 154244978

Following gene: 154244976

Centisome position: 22.24

GC content: 70.49

Gene sequence:

>993_bases
GTGCCGATGCCGTCGCAGCGCAGCTCCCTTTTCGAGGATCAGGTCATGACAGAACAGTTCTCGCCCGAGGTCGCCATTTC
GGGGGCCACAGGGACCGGGGGCGCCGCGGTCATCGGGCTCGGCTCCATGGGCTATGGCATGGCCCAGTCACTGAAGCGCG
CCGGCTTCGACGTGGCCGGCTTCGACGTGAACGCCGCCGCCGTGGAGCGCTTCGCCGCAGAAGGCGGTCGCGGCGCATCG
AGCCCGGCAGAGGCCGCCCGCGACGCCGACGTGGTGGTGAGCGTGGTGGTGAACGCCGCCCAGACCGAGACCATCCTGTT
CGGCGCCGACGGCGTGGCGCAGACGCTGGCAGACGGCGCAGTGTTCCTCTCCTCCGCCACCATGGACCCGGAGATCGCCC
GCACCCTCGCAGCCCGGCTGGAGGCCACAGGCCGGCTCTATCTCGATGCACCCATCTCCGGCGGTGCCCAGCGCGCGGCG
GAAGGCGCGCTCACCATCCTTGCCTCCGGCAGCCCGGCGGCCTTCGCCAAGGCGCGGCCGGCGCTCGATGCCATGGCGGC
GAAGCTCTATGAGCTGGGCGATGCGCCCGGACAGGGCGCGGCTTTCAAGATGATCAACCAGCTGCTCGCCGGCGTGCACA
TCGCGGCGGCGAGCGAGGCCGTCGCGTTTGCCGCGCGGCAGGGGCTCGACATCGCCAAGGTCTATGAGGTCATCACCGCC
TCGGCCGGCAACAGCTGGATGTTCGAGAACCGCATGCCCCACGTGGTGGACGGCGACTATACGCCGAGGAGCGCGGTGGA
CATCTTCGTGAAGGACCTCGGCATCATCCAGGACATGGCCCGCAGCGCGAAATTCCCCGTGCCGGTCTCCGCCGCCGCGC
TGCAGATGTTCCTGGCCACCTCCGCCGCCGGCATGGGGCGCGACGACGACGCCTCCGTCGCCCGCCTCTATGCCCGCATC
ACCGGAACGAAGCTGCCGGGCGATCCGGCCTGA

Upstream 100 bases:

>100_bases
ATCCTGGTGGCCGGCGCCGCTGCGGTGCTCACCACCTCGCCCAAGCTCGTCAACAAGTGAACCCACAGCCTCCCGCGGCG
CGATCCGCGGGAGGCCCCTC

Downstream 100 bases:

>100_bases
GCCGGTGCCGACCTAAACCCTGCTTGCGTCCCTGCGCGGATTGGTGCGTCCTTCACGCCGCACCGACCGCCGCGACCCGA
CCTTTCGGAAGATCCCACCT

Product: 3-hydroxyisobutyrate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 330; Mature: 329

Protein sequence:

>330_residues
MPMPSQRSSLFEDQVMTEQFSPEVAISGATGTGGAAVIGLGSMGYGMAQSLKRAGFDVAGFDVNAAAVERFAAEGGRGAS
SPAEAARDADVVVSVVVNAAQTETILFGADGVAQTLADGAVFLSSATMDPEIARTLAARLEATGRLYLDAPISGGAQRAA
EGALTILASGSPAAFAKARPALDAMAAKLYELGDAPGQGAAFKMINQLLAGVHIAAASEAVAFAARQGLDIAKVYEVITA
SAGNSWMFENRMPHVVDGDYTPRSAVDIFVKDLGIIQDMARSAKFPVPVSAAALQMFLATSAAGMGRDDDASVARLYARI
TGTKLPGDPA

Sequences:

>Translated_330_residues
MPMPSQRSSLFEDQVMTEQFSPEVAISGATGTGGAAVIGLGSMGYGMAQSLKRAGFDVAGFDVNAAAVERFAAEGGRGAS
SPAEAARDADVVVSVVVNAAQTETILFGADGVAQTLADGAVFLSSATMDPEIARTLAARLEATGRLYLDAPISGGAQRAA
EGALTILASGSPAAFAKARPALDAMAAKLYELGDAPGQGAAFKMINQLLAGVHIAAASEAVAFAARQGLDIAKVYEVITA
SAGNSWMFENRMPHVVDGDYTPRSAVDIFVKDLGIIQDMARSAKFPVPVSAAALQMFLATSAAGMGRDDDASVARLYARI
TGTKLPGDPA
>Mature_329_residues
PMPSQRSSLFEDQVMTEQFSPEVAISGATGTGGAAVIGLGSMGYGMAQSLKRAGFDVAGFDVNAAAVERFAAEGGRGASS
PAEAARDADVVVSVVVNAAQTETILFGADGVAQTLADGAVFLSSATMDPEIARTLAARLEATGRLYLDAPISGGAQRAAE
GALTILASGSPAAFAKARPALDAMAAKLYELGDAPGQGAAFKMINQLLAGVHIAAASEAVAFAARQGLDIAKVYEVITAS
AGNSWMFENRMPHVVDGDYTPRSAVDIFVKDLGIIQDMARSAKFPVPVSAAALQMFLATSAAGMGRDDDASVARLYARIT
GTKLPGDPA

Specific function: Unknown

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI23308751, Length=283, Percent_Identity=31.8021201413428, Blast_Score=148, Evalue=7e-36,
Organism=Homo sapiens, GI40556376, Length=280, Percent_Identity=24.6428571428571, Blast_Score=109, Evalue=4e-24,
Organism=Escherichia coli, GI1789092, Length=292, Percent_Identity=49.3150684931507, Blast_Score=257, Evalue=8e-70,
Organism=Escherichia coli, GI145693186, Length=283, Percent_Identity=30.0353356890459, Blast_Score=145, Evalue=5e-36,
Organism=Escherichia coli, GI1790315, Length=279, Percent_Identity=32.6164874551971, Blast_Score=144, Evalue=7e-36,
Organism=Escherichia coli, GI1786719, Length=288, Percent_Identity=26.0416666666667, Blast_Score=114, Evalue=9e-27,
Organism=Caenorhabditis elegans, GI17557316, Length=272, Percent_Identity=30.8823529411765, Blast_Score=116, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24655230, Length=304, Percent_Identity=29.2763157894737, Blast_Score=142, Evalue=4e-34,
Organism=Drosophila melanogaster, GI19922568, Length=304, Percent_Identity=29.2763157894737, Blast_Score=142, Evalue=4e-34,
Organism=Drosophila melanogaster, GI28574115, Length=278, Percent_Identity=25.5395683453237, Blast_Score=109, Evalue=3e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002204
- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR016040 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: 1.1.-.- [C]

Molecular weight: Translated: 33665; Mature: 33534

Theoretical pI: Translated: 4.52; Mature: 4.52

Prosite motif: PS00895 3_HYDROXYISOBUT_DH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPMPSQRSSLFEDQVMTEQFSPEVAISGATGTGGAAVIGLGSMGYGMAQSLKRAGFDVAG
CCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCEEEEECCCCCHHHHHHHHHCCCCEEC
FDVNAAAVERFAAEGGRGASSPAEAARDADVVVSVVVNAAQTETILFGADGVAQTLADGA
CCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHCCH
VFLSSATMDPEIARTLAARLEATGRLYLDAPISGGAQRAAEGALTILASGSPAAFAKARP
HEEECCCCCHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHCCCEEEEECCCCHHHHHHHH
ALDAMAAKLYELGDAPGQGAAFKMINQLLAGVHIAAASEAVAFAARQGLDIAKVYEVITA
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHC
SAGNSWMFENRMPHVVDGDYTPRSAVDIFVKDLGIIQDMARSAKFPVPVSAAALQMFLAT
CCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
SAAGMGRDDDASVARLYARITGTKLPGDPA
HHCCCCCCCHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
PMPSQRSSLFEDQVMTEQFSPEVAISGATGTGGAAVIGLGSMGYGMAQSLKRAGFDVAG
CCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCEEEEECCCCCHHHHHHHHHCCCCEEC
FDVNAAAVERFAAEGGRGASSPAEAARDADVVVSVVVNAAQTETILFGADGVAQTLADGA
CCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHCCH
VFLSSATMDPEIARTLAARLEATGRLYLDAPISGGAQRAAEGALTILASGSPAAFAKARP
HEEECCCCCHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHCCCEEEEECCCCHHHHHHHH
ALDAMAAKLYELGDAPGQGAAFKMINQLLAGVHIAAASEAVAFAARQGLDIAKVYEVITA
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHC
SAGNSWMFENRMPHVVDGDYTPRSAVDIFVKDLGIIQDMARSAKFPVPVSAAALQMFLAT
CCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
SAAGMGRDDDASVARLYARITGTKLPGDPA
HHCCCCCCCHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]