Click here to switch to the map view.

The map label for this gene is dapE [H]

Identifier: 153952252

GI number: 153952252

Start: 623997

End: 625094

Strand: Direct

Name: dapE [H]

Synonym: JJD26997_0688

Alternate gene names: 153952252

Gene position: 623997-625094 (Clockwise)

Preceding gene: 153952097

Following gene: 153951579

Centisome position: 33.82

GC content: 35.7

Gene sequence:

>1098_bases
ATGAATGCAAAAGAATTTTTAATCGAACTTTTAAAATTTAAATCTGTTACGCCAAATGATGATGGAGCTTTAAATTTCAT
CGCTATGGAACTGAGTGATTTTGAAGCTTTTTTTATAGAAAAAGAAGGCATTAAAAACCTTTTACTCACTAAAAAATTTA
AAGATGAGGGTGAGCATTTAGCTTTTGGTGGGCATGTAGATGTGGTGCCTGCAGGCGAAGGTTGGAGTAGCGATGCTTTC
GTGCCTATGGAAAAAGAAAGCTTTATCTATGCAAGGGGTGCTCAAGATATGAAAAGCGGAGTAGCAGCCTTTGTAGATGC
TCTAAAGAATGCGGATTTTAAAGGAGCTAGGCTGAGTCTTATTTTAACAAGTGATGAAGAAGGCGAGGCTATATATGGAA
CCAAGGCTGTTTTAGAATGGATGCAAAAAAGAGATATGCTACCTGATTATGCCGTGGTTGCAGAGCCAACCTGTGTTAAA
AAAATCGGCGATAGTATTAAAATAGGGCGTCGTGGTTCTATCAATGGAAAGCTTTTAATCCGTGGAAAACAAGGGCATGT
TGCTTATCCTGAAAAATGTATCAATCCTGTGCATGATTTTGCTCCAGTGTTAAAGCTTTTAGCGGGTTTTGATCTTGATC
CTGGAAGTGCTGAATTTAGTCCTTCAAAAATCGTTGTTACCGATATTCGCGGAGGTATAGAGGTGTGTAATGTTACGCCA
AATGATTTAAAACTTATGTTTAATGTTCGCAATTCTCCTGATACAAGCTTAGAAGATGTAAAAAGCTATGTAGAAAAAAT
TTGTCATGGTTTAAACTATGAGTTAGAGCTAAAACAATCAAGTGAAGCTTTTTTGACAAATATTGACAATAAAATCGTTC
AAAAAATGAATGAAAGCGTTCAAAAAATCACTCATGAAGTACCAGAGCTTAATACCAAAGGTGGCACAAGTGATGCAAGG
TATTTTGCAAAATACGGTGTAAAGGTAGTGGAATTTGGGGTTTGTAATGATAGAATTCACGCTATTGATGAAAGAGTAAG
CATAGAAGAATTTGAAAAACTTTGCCTTGTTTTTAAAGACTTGATAGAAAATTTTTAA

Upstream 100 bases:

>100_bases
TTTTAGTGCAAAAGTTGTTTTTATAATCAATATAATTTCAGCTATAATTATAGAGTATTTTGCTCTTAATTTATTATATA
AAACTTTCTTAGGATAAAAA

Downstream 100 bases:

>100_bases
AGGTTTAGAATGATTGAGGAAATTTTAAAAGATAGTGATTACAAGCTAGATCTATTTAGTAAAAAAGCTATTGCTGAATT
AGAAACTAAAATTATAGTTA

Product: succinyl-diaminopimelate desuccinylase

Products: NA

Alternate protein names: SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase [H]

Number of amino acids: Translated: 365; Mature: 365

Protein sequence:

>365_residues
MNAKEFLIELLKFKSVTPNDDGALNFIAMELSDFEAFFIEKEGIKNLLLTKKFKDEGEHLAFGGHVDVVPAGEGWSSDAF
VPMEKESFIYARGAQDMKSGVAAFVDALKNADFKGARLSLILTSDEEGEAIYGTKAVLEWMQKRDMLPDYAVVAEPTCVK
KIGDSIKIGRRGSINGKLLIRGKQGHVAYPEKCINPVHDFAPVLKLLAGFDLDPGSAEFSPSKIVVTDIRGGIEVCNVTP
NDLKLMFNVRNSPDTSLEDVKSYVEKICHGLNYELELKQSSEAFLTNIDNKIVQKMNESVQKITHEVPELNTKGGTSDAR
YFAKYGVKVVEFGVCNDRIHAIDERVSIEEFEKLCLVFKDLIENF

Sequences:

>Translated_365_residues
MNAKEFLIELLKFKSVTPNDDGALNFIAMELSDFEAFFIEKEGIKNLLLTKKFKDEGEHLAFGGHVDVVPAGEGWSSDAF
VPMEKESFIYARGAQDMKSGVAAFVDALKNADFKGARLSLILTSDEEGEAIYGTKAVLEWMQKRDMLPDYAVVAEPTCVK
KIGDSIKIGRRGSINGKLLIRGKQGHVAYPEKCINPVHDFAPVLKLLAGFDLDPGSAEFSPSKIVVTDIRGGIEVCNVTP
NDLKLMFNVRNSPDTSLEDVKSYVEKICHGLNYELELKQSSEAFLTNIDNKIVQKMNESVQKITHEVPELNTKGGTSDAR
YFAKYGVKVVEFGVCNDRIHAIDERVSIEEFEKLCLVFKDLIENF
>Mature_365_residues
MNAKEFLIELLKFKSVTPNDDGALNFIAMELSDFEAFFIEKEGIKNLLLTKKFKDEGEHLAFGGHVDVVPAGEGWSSDAF
VPMEKESFIYARGAQDMKSGVAAFVDALKNADFKGARLSLILTSDEEGEAIYGTKAVLEWMQKRDMLPDYAVVAEPTCVK
KIGDSIKIGRRGSINGKLLIRGKQGHVAYPEKCINPVHDFAPVLKLLAGFDLDPGSAEFSPSKIVVTDIRGGIEVCNVTP
NDLKLMFNVRNSPDTSLEDVKSYVEKICHGLNYELELKQSSEAFLTNIDNKIVQKMNESVQKITHEVPELNTKGGTSDAR
YFAKYGVKVVEFGVCNDRIHAIDERVSIEEFEKLCLVFKDLIENF

Specific function: Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bact

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788816, Length=370, Percent_Identity=34.5945945945946, Blast_Score=215, Evalue=3e-57,
Organism=Escherichia coli, GI1790395, Length=141, Percent_Identity=30.4964539007092, Blast_Score=69, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001261
- InterPro:   IPR005941
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.18 [H]

Molecular weight: Translated: 40603; Mature: 40603

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: PS00758 ARGE_DAPE_CPG2_1 ; PS00759 ARGE_DAPE_CPG2_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAKEFLIELLKFKSVTPNDDGALNFIAMELSDFEAFFIEKEGIKNLLLTKKFKDEGEHL
CCHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCEEEEEHHHHHHHHHHHHHHCCCCEE
AFGGHVDVVPAGEGWSSDAFVPMEKESFIYARGAQDMKSGVAAFVDALKNADFKGARLSL
EECCEEEEEECCCCCCCCCEEECCCCCEEEECCCHHHHHHHHHHHHHHHCCCCCCCEEEE
ILTSDEEGEAIYGTKAVLEWMQKRDMLPDYAVVAEPTCVKKIGDSIKIGRRGSINGKLLI
EEECCCCCCEEEHHHHHHHHHHHHCCCCCCCEECCCHHHHHCCCCEECCCCCCCCCEEEE
RGKQGHVAYPEKCINPVHDFAPVLKLLAGFDLDPGSAEFSPSKIVVTDIRGGIEVCNVTP
EECCCCCCCCHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCEEEEECCC
NDLKLMFNVRNSPDTSLEDVKSYVEKICHGLNYELELKQSSEAFLTNIDNKIVQKMNESV
CCEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHH
QKITHEVPELNTKGGTSDARYFAKYGVKVVEFGVCNDRIHAIDERVSIEEFEKLCLVFKD
HHHHHHCCCCCCCCCCHHHHHHHHCCCEEEEEECCCCHHHHHHHHCCHHHHHHHHHHHHH
LIENF
HHHCC
>Mature Secondary Structure
MNAKEFLIELLKFKSVTPNDDGALNFIAMELSDFEAFFIEKEGIKNLLLTKKFKDEGEHL
CCHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCEEEEEHHHHHHHHHHHHHHCCCCEE
AFGGHVDVVPAGEGWSSDAFVPMEKESFIYARGAQDMKSGVAAFVDALKNADFKGARLSL
EECCEEEEEECCCCCCCCCEEECCCCCEEEECCCHHHHHHHHHHHHHHHCCCCCCCEEEE
ILTSDEEGEAIYGTKAVLEWMQKRDMLPDYAVVAEPTCVKKIGDSIKIGRRGSINGKLLI
EEECCCCCCEEEHHHHHHHHHHHHCCCCCCCEECCCHHHHHCCCCEECCCCCCCCCEEEE
RGKQGHVAYPEKCINPVHDFAPVLKLLAGFDLDPGSAEFSPSKIVVTDIRGGIEVCNVTP
EECCCCCCCCHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCEEEEECCC
NDLKLMFNVRNSPDTSLEDVKSYVEKICHGLNYELELKQSSEAFLTNIDNKIVQKMNESV
CCEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHH
QKITHEVPELNTKGGTSDARYFAKYGVKVVEFGVCNDRIHAIDERVSIEEFEKLCLVFKD
HHHHHHCCCCCCCCCCHHHHHHHHCCCEEEEEECCCCHHHHHHHHCCHHHHHHHHHHHHH
LIENF
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA