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The map label for this gene is cwlA [H]

Identifier: 152976640

GI number: 152976640

Start: 2989573

End: 2990394

Strand: Reverse

Name: cwlA [H]

Synonym: Bcer98_2931

Alternate gene names: 152976640

Gene position: 2990394-2989573 (Counterclockwise)

Preceding gene: 152976641

Following gene: 152976637

Centisome position: 73.17

GC content: 39.05

Gene sequence:

>822_bases
ATGGGACACATTGTAGATATTTCAAAATGGAATGGTGACATTAACTGGCCTATAGCAAAGCAATACATTGATTTCATCAT
CGCTCGTGTACAAGATGGTTCAAATTATGTAGATCCATTGTATAAAGGATATGTACAAGCCATGAAGCAACATGGTATTC
CTTTTGGTAACTATGCATTCTGTCGTTTCGTTTCTGAAAATGATGCAAGAATAGAAGCGCGTGACTTCTGGAACCGTGGA
GATAAGAGCGCGACAGTATGGGTTGCGGATGTTGAAGTGAAAACAATGAATGATATGAGAGCGGGCACACAAGCATTTAT
TGATGAACTACGCCGATTAGGTGCTCAGAAAGTTGGTTTATACGTTGGTCATCATATGTATGCTCCGTTTGGTATGGCAA
ATGTAAAATCTGACTTTGTTTGGATTCCTCGTTATGGCGGTAACAAACCTGCATATGAGTGCGATATTTGGCAATACACA
GAGACAGGGCACGTGCCTGGTATTGGTAAATGCGATTTAAATAAATTAATCGGAAATAAATCGCTGGCTTGGTTTACAAA
TCAAACTGTTGAAAAACAAGAAGGAGTGGGGATTAAAGTGGATAAATTTAATAAAGTCGTATCGTATGAATTTGGTACAG
CATTAGTGCCGGAAATGTTAGGGATGATGGATGCTCTTGGTTATGAATCTCGTATCATCTCTTATGGAGATAAACAAGGG
TTAGTTAGATTTGAAACAGCATACCGCCAAGGGAATGAACTAGATCGAGCAACAGCGTGGTTAGATGCTAAAGGTATTGA
TTACGTCTATACACAAGAATAG

Upstream 100 bases:

>100_bases
TTCCCCTTCCACAAGCATTAACAAATGCAGTTGAAATTTTAAGTAATAAGAGCAATAAGACAAGCTCTGAATATGATAAT
AAAAAAGGAGATGTTGAGTA

Downstream 100 bases:

>100_bases
TTTGATTAACAAAAATAAGAGCCGTCCTGTTGGGCGGCTTCTTTTTTATGGCTTTTTATTGTCCTAAGCCATTATTACCT
CTTTTTTTTGAGATGTTTCG

Product: glycoside hydrolase family protein

Products: NA

Alternate protein names: Autolysin; Cell wall hydrolase [H]

Number of amino acids: Translated: 273; Mature: 272

Protein sequence:

>273_residues
MGHIVDISKWNGDINWPIAKQYIDFIIARVQDGSNYVDPLYKGYVQAMKQHGIPFGNYAFCRFVSENDARIEARDFWNRG
DKSATVWVADVEVKTMNDMRAGTQAFIDELRRLGAQKVGLYVGHHMYAPFGMANVKSDFVWIPRYGGNKPAYECDIWQYT
ETGHVPGIGKCDLNKLIGNKSLAWFTNQTVEKQEGVGIKVDKFNKVVSYEFGTALVPEMLGMMDALGYESRIISYGDKQG
LVRFETAYRQGNELDRATAWLDAKGIDYVYTQE

Sequences:

>Translated_273_residues
MGHIVDISKWNGDINWPIAKQYIDFIIARVQDGSNYVDPLYKGYVQAMKQHGIPFGNYAFCRFVSENDARIEARDFWNRG
DKSATVWVADVEVKTMNDMRAGTQAFIDELRRLGAQKVGLYVGHHMYAPFGMANVKSDFVWIPRYGGNKPAYECDIWQYT
ETGHVPGIGKCDLNKLIGNKSLAWFTNQTVEKQEGVGIKVDKFNKVVSYEFGTALVPEMLGMMDALGYESRIISYGDKQG
LVRFETAYRQGNELDRATAWLDAKGIDYVYTQE
>Mature_272_residues
GHIVDISKWNGDINWPIAKQYIDFIIARVQDGSNYVDPLYKGYVQAMKQHGIPFGNYAFCRFVSENDARIEARDFWNRGD
KSATVWVADVEVKTMNDMRAGTQAFIDELRRLGAQKVGLYVGHHMYAPFGMANVKSDFVWIPRYGGNKPAYECDIWQYTE
TGHVPGIGKCDLNKLIGNKSLAWFTNQTVEKQEGVGIKVDKFNKVVSYEFGTALVPEMLGMMDALGYESRIISYGDKQGL
VRFETAYRQGNELDRATAWLDAKGIDYVYTQE

Specific function: Autolysins are involved in some important biological processes such as cell separation, cell-wall turnover, competence for genetic transformation, formation of the flagella and sporulation [H]

COG id: COG3757

COG function: function code M; Lyzozyme M1 (1,4-beta-N-acetylmuramidase)

Gene ontology:

Cell location: Secreted (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR021976
- InterPro:   IPR002502 [H]

Pfam domain/function: PF12123 Amidase02_C; PF01510 Amidase_2 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 31038; Mature: 30906

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGHIVDISKWNGDINWPIAKQYIDFIIARVQDGSNYVDPLYKGYVQAMKQHGIPFGNYAF
CCCEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCEEE
CRFVSENDARIEARDFWNRGDKSATVWVADVEVKTMNDMRAGTQAFIDELRRLGAQKVGL
EEEECCCCCEEEHHHHHCCCCCCEEEEEEEEEEEEHHHHHCCHHHHHHHHHHHCHHHHHH
YVGHHMYAPFGMANVKSDFVWIPRYGGNKPAYECDIWQYTETGHVPGIGKCDLNKLIGNK
EECCHHCCCCCCCCCCCCEEEEECCCCCCCCEEEEEEEECCCCCCCCCCCCCHHHHHCCC
SLAWFTNQTVEKQEGVGIKVDKFNKVVSYEFGTALVPEMLGMMDALGYESRIISYGDKQG
CEEEEECCCHHHHCCCCEEECCCCCEEEECCCHHHHHHHHHHHHHCCCCHHEEECCCCCC
LVRFETAYRQGNELDRATAWLDAKGIDYVYTQE
CEEEEHHHHCCCCHHHHHHEECCCCCCEEEECC
>Mature Secondary Structure 
GHIVDISKWNGDINWPIAKQYIDFIIARVQDGSNYVDPLYKGYVQAMKQHGIPFGNYAF
CCEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCEEE
CRFVSENDARIEARDFWNRGDKSATVWVADVEVKTMNDMRAGTQAFIDELRRLGAQKVGL
EEEECCCCCEEEHHHHHCCCCCCEEEEEEEEEEEEHHHHHCCHHHHHHHHHHHCHHHHHH
YVGHHMYAPFGMANVKSDFVWIPRYGGNKPAYECDIWQYTETGHVPGIGKCDLNKLIGNK
EECCHHCCCCCCCCCCCCEEEEECCCCCCCCEEEEEEEECCCCCCCCCCCCCHHHHHCCC
SLAWFTNQTVEKQEGVGIKVDKFNKVVSYEFGTALVPEMLGMMDALGYESRIISYGDKQG
CEEEEECCCHHHHCCCCEEECCCCCEEEECCCHHHHHHHHHHHHHCCCCHHEEECCCCCC
LVRFETAYRQGNELDRATAWLDAKGIDYVYTQE
CEEEEHHHHCCCCHHHHHHEECCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3070348 [H]