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The map label for this gene is comGA [H]

Identifier: 152976637

GI number: 152976637

Start: 2987217

End: 2987801

Strand: Reverse

Name: comGA [H]

Synonym: Bcer98_2928

Alternate gene names: 152976637

Gene position: 2987801-2987217 (Counterclockwise)

Preceding gene: 152976640

Following gene: 152976636

Centisome position: 73.1

GC content: 38.97

Gene sequence:

>585_bases
ATGTATGCGTTATTAGAAGTTGCCAAAAAAGGTCAAACACGCCGGATTGTTACATTGGAAGATCCGGTGGAGAAAAGAAA
GAACGGTTTATTACAAATTCAAATTAATGAAAAAGCAGGGATTACGTATGAGACAGGTTTAAAGGCAATTTTACGGCATG
ACCCAGATATTATTTTAGTGGGGGAAATTCGTGATGAAGAAACAGCAAAAGTGGCTGTACGTGCAAGTTTAACAGGTCAT
TTAGTAATGACAACGCTGCATACAAATGATGCGAAAGGTGCTGTATTGCGCTTGATGGATTTTGGAATTAGCAGACAGGA
AATTGAACAGTCGTTATTAGCTGTAGCCGCTCAGCGCCTTGTAGAGTTGAAATGTCCGTTTTGTAAAGGGAAGTGTTCAC
CATTATGTAAATCGATACGCCAAGTACGGCAAGCAAGCATTTATGAGTTGCTATATGGTCATGAATTGAAGAAAGCCATT
CGCGAAGCGAATGGCGAACGTGTGGTGTATCGCTATGAAACATTACAATCTTCATTAAAGAAAGGCTATGCTTTAGGATT
TTTAGAAGAGGATGTTTATGTTTAA

Upstream 100 bases:

>100_bases
GTTTTCATTGTGTATCCCTCGCTATCCGTGGTTTGATTATCTTTTTATTCGGAGTTGTATTTATGTTACTAAAACAACTG
GATCTGGAAAAACAACAACA

Downstream 100 bases:

>100_bases
TAGAAAAAAATGGCGATTAGATGAACAAGTTATGCTTTTAAAAAGGTTAGGAGATTTATTGGAAAAAGGCTATTCGCTCT
TACAAGCGTTAGAGTTTTTA

Product: type II secretion system protein E

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 194; Mature: 194

Protein sequence:

>194_residues
MYALLEVAKKGQTRRIVTLEDPVEKRKNGLLQIQINEKAGITYETGLKAILRHDPDIILVGEIRDEETAKVAVRASLTGH
LVMTTLHTNDAKGAVLRLMDFGISRQEIEQSLLAVAAQRLVELKCPFCKGKCSPLCKSIRQVRQASIYELLYGHELKKAI
REANGERVVYRYETLQSSLKKGYALGFLEEDVYV

Sequences:

>Translated_194_residues
MYALLEVAKKGQTRRIVTLEDPVEKRKNGLLQIQINEKAGITYETGLKAILRHDPDIILVGEIRDEETAKVAVRASLTGH
LVMTTLHTNDAKGAVLRLMDFGISRQEIEQSLLAVAAQRLVELKCPFCKGKCSPLCKSIRQVRQASIYELLYGHELKKAI
REANGERVVYRYETLQSSLKKGYALGFLEEDVYV
>Mature_194_residues
MYALLEVAKKGQTRRIVTLEDPVEKRKNGLLQIQINEKAGITYETGLKAILRHDPDIILVGEIRDEETAKVAVRASLTGH
LVMTTLHTNDAKGAVLRLMDFGISRQEIEQSLLAVAAQRLVELKCPFCKGKCSPLCKSIRQVRQASIYELLYGHELKKAI
REANGERVVYRYETLQSSLKKGYALGFLEEDVYV

Specific function: Required for uptake of DNA by competent cells [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=129, Percent_Identity=51.937984496124, Blast_Score=138, Evalue=3e-34,
Organism=Escherichia coli, GI1786296, Length=114, Percent_Identity=49.1228070175439, Blast_Score=121, Evalue=4e-29,
Organism=Escherichia coli, GI87082188, Length=114, Percent_Identity=44.7368421052632, Blast_Score=82, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 21859; Mature: 21859

Theoretical pI: Translated: 9.10; Mature: 9.10

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYALLEVAKKGQTRRIVTLEDPVEKRKNGLLQIQINEKAGITYETGLKAILRHDPDIILV
CHHHHHHHHCCCCEEEEEECCHHHHHHCCEEEEEECCCCCCCHHHHHHHHHHCCCCEEEE
GEIRDEETAKVAVRASLTGHLVMTTLHTNDAKGAVLRLMDFGISRQEIEQSLLAVAAQRL
ECCCCCCHHEEEEEEECCCEEEEEEEECCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHH
VELKCPFCKGKCSPLCKSIRQVRQASIYELLYGHELKKAIREANGERVVYRYETLQSSLK
HHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHH
KGYALGFLEEDVYV
CCCEEEECCCCCCC
>Mature Secondary Structure
MYALLEVAKKGQTRRIVTLEDPVEKRKNGLLQIQINEKAGITYETGLKAILRHDPDIILV
CHHHHHHHHCCCCEEEEEECCHHHHHHCCEEEEEECCCCCCCHHHHHHHHHHCCCCEEEE
GEIRDEETAKVAVRASLTGHLVMTTLHTNDAKGAVLRLMDFGISRQEIEQSLLAVAAQRL
ECCCCCCHHEEEEEEECCCEEEEEEEECCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHH
VELKCPFCKGKCSPLCKSIRQVRQASIYELLYGHELKKAIREANGERVVYRYETLQSSLK
HHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHH
KGYALGFLEEDVYV
CCCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2507524; 8969508; 9384377; 9422590; 9723928 [H]